YU73494A - Postupak za dobijanje proteina - Google Patents

Postupak za dobijanje proteina

Info

Publication number
YU73494A
YU73494A YU73494A YU73494A YU73494A YU 73494 A YU73494 A YU 73494A YU 73494 A YU73494 A YU 73494A YU 73494 A YU73494 A YU 73494A YU 73494 A YU73494 A YU 73494A
Authority
YU
Yugoslavia
Prior art keywords
procedure
cell lines
degrees centigrade
recombinant proteins
obtaining protein
Prior art date
Application number
YU73494A
Other languages
English (en)
Inventor
William Grinnell Brian
Original Assignee
Eli Lilly And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly And Company filed Critical Eli Lilly And Company
Publication of YU73494A publication Critical patent/YU73494A/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Abstract

Opisan je postupak za povećanje proizvodnje proteina C u adenovirus-transformisanoj rekombinantnoj ćeliji domaćina sisara, koji obuhvata gajenje adenovirus-transformisane rekombinantne ćelije domaćina sisara, ćelije 293, na temperaturi izmedju oko 38В°C i oko 39В°C. Takođe je opisan postupak za povećanje nivoa gama-karboksilacije rekombinantno proizvedenog molekula proteina C, koji obuhvata gajenje rekombinantno proizvedenog molekula proteina C u rekombinantnoj domaćoj AV12 ćeliji na temperaturi između oko 38В°C i oko 39В°C.
YU73494A 1993-12-15 1994-12-14 Postupak za dobijanje proteina YU73494A (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/168,035 US5618714A (en) 1993-12-15 1993-12-15 Methods for producing protein C

Publications (1)

Publication Number Publication Date
YU73494A true YU73494A (sh) 1997-03-07

Family

ID=22609820

Family Applications (1)

Application Number Title Priority Date Filing Date
YU73494A YU73494A (sh) 1993-12-15 1994-12-14 Postupak za dobijanje proteina

Country Status (28)

Country Link
US (1) US5618714A (sh)
EP (1) EP0658626B1 (sh)
JP (1) JP3662614B2 (sh)
KR (1) KR950018454A (sh)
CN (1) CN1107887A (sh)
AT (1) ATE196165T1 (sh)
AU (1) AU687914B2 (sh)
BR (1) BR9404991A (sh)
CA (1) CA2138101A1 (sh)
CO (1) CO4340654A1 (sh)
CZ (2) CZ315794A3 (sh)
DE (1) DE69425809T2 (sh)
DK (1) DK0658626T3 (sh)
ES (1) ES2149244T3 (sh)
FI (1) FI106385B (sh)
GR (1) GR3034909T3 (sh)
HU (1) HU219508B (sh)
IL (1) IL111983A0 (sh)
NO (1) NO944846L (sh)
NZ (1) NZ270140A (sh)
PE (1) PE42695A1 (sh)
PL (1) PL176381B1 (sh)
PT (1) PT658626E (sh)
RU (1) RU2110574C1 (sh)
SI (1) SI0658626T1 (sh)
UA (1) UA26870C2 (sh)
YU (1) YU73494A (sh)
ZA (1) ZA949981B (sh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19724543A1 (de) * 1997-06-11 1998-12-17 Philips Patentverwaltung Wechsler-Gerät für Informationsplatten
EP1998266A3 (en) 2001-02-19 2009-02-11 Merck Patent GmbH Method for identification of T-cell epitopes and use for preparing molecules with reduced immunogenicity
EP1492891B1 (en) * 2002-03-29 2008-02-20 Merck & Co., Inc. Methods of virus production
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
JP2008507561A (ja) * 2004-07-23 2008-03-13 ザ ユニバーシティ オブ ロチェスター 活性化プロテインｃによる、脳内のプラスミノゲン活性化因子の不都合な作用の阻害
CA2612859A1 (en) * 2005-06-23 2006-12-28 The University Of British Columbia Coagulation factor iii polymorphisms associated with prediction of subject outcome and response to therapy
EP2041338A4 (en) * 2006-06-09 2009-09-30 Univ British Columbia INTERFERON GAMMA POLYMORPHISMS AS INDICATORS OF PATIENT RESULTS IN FATIGUE PATIENTS
WO2009089620A1 (en) * 2008-01-15 2009-07-23 The Universityof British Columbia Protein c rs2069915 as a response predictor to survival and administration of activated protein c or protein c-like compound
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
US4968626A (en) * 1985-08-15 1990-11-06 Board Of Reagents Of The University Of Washington DNA sequence coding for protein C
US4959318A (en) * 1985-06-27 1990-09-25 Zymogenetics, Inc. Expression of protein C
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
JPH01502080A (ja) * 1986-11-17 1989-07-27 ニュー・イングランド・メディカル・センター 組み換えビタミンｋ依存性蛋白質のガンマカルボキシル化の増強
US4992373A (en) * 1987-12-04 1991-02-12 Eli Lilly And Company Vectors and compounds for direct expression of activated human protein C
ZA889497B (en) * 1987-12-28 1990-08-29 Lilly Co Eli Vectors and compounds for expression of zymogen forms of human protein c
JPH0246296A (ja) * 1988-08-09 1990-02-15 Hoechst Japan Ltd 雑種プロテインｃ及びその製造方法
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA1332049C (en) * 1988-10-07 1994-09-20 Eli Lilly And Company Eukaryotic expression
WO1991002065A1 (en) * 1989-08-11 1991-02-21 Zymogenetics, Inc. Cell culture methods for producing activated protein c
IL97312A (en) * 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
CA2078667A1 (en) * 1991-01-29 1992-07-30 Masahiko Suzuki Human protein c expression vector

Also Published As

Publication number Publication date
ATE196165T1 (de) 2000-09-15
KR950018454A (ko) 1995-07-22
HU9403600D0 (en) 1995-02-28
NZ270140A (en) 1996-05-28
US5618714A (en) 1997-04-08
PT658626E (pt) 2001-01-31
ZA949981B (en) 1996-06-14
AU8045294A (en) 1995-06-22
FI106385B (fi) 2001-01-31
EP0658626A1 (en) 1995-06-21
CN1107887A (zh) 1995-09-06
CZ315794A3 (en) 1995-10-18
PL176381B1 (pl) 1999-05-31
CZ290724B6 (cs) 2002-10-16
JP3662614B2 (ja) 2005-06-22
IL111983A0 (en) 1995-03-15
AU687914B2 (en) 1998-03-05
EP0658626B1 (en) 2000-09-06
BR9404991A (pt) 1995-08-08
NO944846D0 (no) 1994-12-14
PL306291A1 (en) 1995-06-26
HU219508B (hu) 2001-04-28
CA2138101A1 (en) 1995-06-16
JPH07222589A (ja) 1995-08-22
UA26870C2 (uk) 1999-12-29
FI945877A0 (fi) 1994-12-14
DE69425809D1 (de) 2000-10-12
RU2110574C1 (ru) 1998-05-10
FI945877A (fi) 1995-06-16
HUT69979A (en) 1995-09-28
SI0658626T1 (en) 2001-02-28
RU94043769A (ru) 1997-03-27
ES2149244T3 (es) 2000-11-01
CO4340654A1 (es) 1996-07-30
PE42695A1 (es) 1995-12-12
DE69425809T2 (de) 2001-02-01
NO944846L (no) 1995-06-16
DK0658626T3 (da) 2000-10-16
GR3034909T3 (en) 2001-02-28

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