DE69633343D1 - Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar - Google Patents
Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbarInfo
- Publication number
- DE69633343D1 DE69633343D1 DE69633343T DE69633343T DE69633343D1 DE 69633343 D1 DE69633343 D1 DE 69633343D1 DE 69633343 T DE69633343 T DE 69633343T DE 69633343 T DE69633343 T DE 69633343T DE 69633343 D1 DE69633343 D1 DE 69633343D1
- Authority
- DE
- Germany
- Prior art keywords
- seqidno
- activity
- phaffia
- enzyme
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
- C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP95203620 | 1995-12-22 | ||
EP95203620 | 1995-12-22 | ||
EP96200943 | 1996-04-11 | ||
EP96200943 | 1996-04-11 | ||
PCT/EP1996/005887 WO1997023633A1 (en) | 1995-12-22 | 1996-12-23 | Improved methods for transforming phaffia strains, transformed phaffia strains so obtained and recombinant dna in said methods |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69633343D1 true DE69633343D1 (de) | 2004-10-14 |
DE69633343T2 DE69633343T2 (de) | 2005-09-22 |
Family
ID=26139955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69633343T Expired - Lifetime DE69633343T2 (de) | 1995-12-22 | 1996-12-23 | Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar |
Country Status (9)
Country | Link |
---|---|
US (2) | US6329141B1 (de) |
EP (2) | EP0870042B1 (de) |
JP (1) | JP2000507087A (de) |
AT (1) | ATE275632T1 (de) |
AU (1) | AU725340B2 (de) |
CA (1) | CA2241267C (de) |
DE (1) | DE69633343T2 (de) |
ES (1) | ES2229288T3 (de) |
WO (1) | WO1997023633A1 (de) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3151371B2 (ja) | 1995-03-10 | 2001-04-03 | 麒麟麦酒株式会社 | カロチノイド生産量の増量に有用なdna鎖 |
CA2241267C (en) * | 1995-12-22 | 2012-12-04 | Gist-Brocades B.V. | Improved methods for transforming phaffia strains, transformed phaffia strains so obtained and recombinant dna in said methods |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
EP0955363A3 (de) * | 1998-05-06 | 2004-01-28 | F. Hoffmann-La Roche Ag | DNS Sequenzen kodierend für Enzyme für die Herstellung von Isoprenoiden |
TWI250210B (en) | 1998-05-06 | 2006-03-01 | Dsm Ip Assets Bv | An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate |
EP2305825B1 (de) | 1998-07-06 | 2015-01-14 | DCV Inc. doing business as Bio-Technical Resourses | Verfahren zur Vitaminherstellung |
MX219130B (es) * | 1998-07-06 | 2004-02-10 | Dcv Inc Haciendo Negocios Como | Metodo para producir vitamina. |
KR100316741B1 (ko) | 1998-10-31 | 2002-09-04 | 한국생명공학연구원 | 파피아로도지마형질전환용벡터및그형질전환방법 |
DK1035206T3 (da) * | 1999-03-09 | 2004-02-16 | Hoffmann La Roche | Astaxanthinsynthetase |
AUPQ262599A0 (en) * | 1999-09-02 | 1999-09-23 | University Of Queensland, The | Gene expression |
KR100371264B1 (ko) * | 2000-01-25 | 2003-02-05 | 양문식 | 무라인 그래뉼로사이트-매크로파지 콜로니 자극 인자를포함하는 재조합 벡터와 그것을 발현하는 재조합아스퍼질러스 나이거 |
AU784237B2 (en) * | 2000-05-24 | 2006-02-23 | Dsm Ip Assets B.V. | Process for astaxanthin |
US6818424B2 (en) | 2000-09-01 | 2004-11-16 | E. I. Du Pont De Nemours And Company | Production of cyclic terpenoids |
US6660507B2 (en) | 2000-09-01 | 2003-12-09 | E. I. Du Pont De Nemours And Company | Genes involved in isoprenoid compound production |
US7081344B2 (en) | 2000-11-30 | 2006-07-25 | Millennium Pharmaceuticals, Inc. | Methods for screening compounds that bind human COE-2 |
CN101006174B (zh) * | 2004-06-21 | 2010-05-26 | 诺维信公司 | 以相同取向稳定保持的至少两个orf的多拷贝 |
CN103589650A (zh) | 2005-03-18 | 2014-02-19 | 米克罗比亚公司 | 产油酵母和真菌中类胡萝卜素的产生 |
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
TW201329234A (zh) * | 2011-11-28 | 2013-07-16 | Daiichi Sankyo Co Ltd | 源自人類基因的啟動子 |
JP2015226469A (ja) * | 2012-09-24 | 2015-12-17 | 株式会社カネカ | 新規dna、およびそれを利用したカロテノイドの製造方法 |
CA2949381A1 (en) * | 2014-05-16 | 2015-11-19 | Academia Sinica | Recombinant polynucleotide sequence for producing astaxanthin and uses thereof |
WO2020162959A1 (en) | 2019-02-07 | 2020-08-13 | The General Hospital Corporation | Carotenoids for treating or preventing nausea |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1341302C (en) | 1983-02-22 | 2001-10-09 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein |
EP0480480A3 (en) | 1984-05-11 | 1992-06-10 | Chiron Corporation | Enhanced yeast transcription employing hybrid promoter region constructs |
CA2047000A1 (en) * | 1990-07-20 | 1992-01-21 | John W. Chapman | Astaxanthin-generating yeast cells |
GB2254536B (en) | 1991-04-08 | 1994-10-05 | Petrella Ltd | A food product |
DK115191D0 (da) * | 1991-06-14 | 1991-06-14 | Ingrid Stampe Villadsen | Fremgangsmaade til frembringelse af carotenoidproducerende, isaer astaxanthinproducerende, mikroorganismer, mikroorganismer opnaaet ved fremgangsmaaden og fremgangsmaade til fremstilling af carotenoidholdige mikroorganismeceller eller -celledele eller oprenset carotenoid |
JPH05168465A (ja) * | 1991-12-25 | 1993-07-02 | Lion Corp | ファフィア・ロドチーマの突然変異株およびその培養によるカロチノイド類の製造方法 |
NZ248628A (en) * | 1992-09-11 | 1996-02-27 | Gist Brocades Nv | Transformed phaffia (yeast) strains and methods and vectors used |
JP3151371B2 (ja) * | 1995-03-10 | 2001-04-03 | 麒麟麦酒株式会社 | カロチノイド生産量の増量に有用なdna鎖 |
CA2241267C (en) * | 1995-12-22 | 2012-12-04 | Gist-Brocades B.V. | Improved methods for transforming phaffia strains, transformed phaffia strains so obtained and recombinant dna in said methods |
-
1996
- 1996-12-23 CA CA2241267A patent/CA2241267C/en not_active Expired - Lifetime
- 1996-12-23 AT AT96944694T patent/ATE275632T1/de not_active IP Right Cessation
- 1996-12-23 JP JP09523340A patent/JP2000507087A/ja active Pending
- 1996-12-23 EP EP96944694A patent/EP0870042B1/de not_active Expired - Lifetime
- 1996-12-23 AU AU13087/97A patent/AU725340B2/en not_active Ceased
- 1996-12-23 WO PCT/EP1996/005887 patent/WO1997023633A1/en active IP Right Grant
- 1996-12-23 EP EP04075855A patent/EP1640456A3/de not_active Withdrawn
- 1996-12-23 DE DE69633343T patent/DE69633343T2/de not_active Expired - Lifetime
- 1996-12-23 ES ES96944694T patent/ES2229288T3/es not_active Expired - Lifetime
- 1996-12-23 US US09/091,725 patent/US6329141B1/en not_active Expired - Lifetime
-
2001
- 2001-10-30 US US10/001,192 patent/US7205123B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP1640456A3 (de) | 2008-10-01 |
US7205123B2 (en) | 2007-04-17 |
US20040091958A1 (en) | 2004-05-13 |
EP0870042B1 (de) | 2004-09-08 |
ATE275632T1 (de) | 2004-09-15 |
ES2229288T3 (es) | 2005-04-16 |
AU1308797A (en) | 1997-07-17 |
US6329141B1 (en) | 2001-12-11 |
AU725340B2 (en) | 2000-10-12 |
CA2241267C (en) | 2012-12-04 |
EP1640456A2 (de) | 2006-03-29 |
CA2241267A1 (en) | 1997-07-03 |
EP0870042A1 (de) | 1998-10-14 |
WO1997023633A1 (en) | 1997-07-03 |
DE69633343T2 (de) | 2005-09-22 |
JP2000507087A (ja) | 2000-06-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |