ATE275632T1 - Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar - Google Patents

Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar

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Publication number
ATE275632T1
ATE275632T1 AT96944694T AT96944694T ATE275632T1 AT E275632 T1 ATE275632 T1 AT E275632T1 AT 96944694 T AT96944694 T AT 96944694T AT 96944694 T AT96944694 T AT 96944694T AT E275632 T1 ATE275632 T1 AT E275632T1
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Austria
Prior art keywords
seqidno
activity
phaffifa
strains
phaffia
Prior art date
Application number
AT96944694T
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English (en)
Inventor
Ooijen Albert Johannes Jos Van
Jan Cornelis Verdoes
Jan Wery
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Dsm Ip Assets Bv
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Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
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Publication of ATE275632T1 publication Critical patent/ATE275632T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
AT96944694T 1995-12-22 1996-12-23 Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar ATE275632T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP95203620 1995-12-22
EP96200943 1996-04-11
PCT/EP1996/005887 WO1997023633A1 (en) 1995-12-22 1996-12-23 Improved methods for transforming phaffia strains, transformed phaffia strains so obtained and recombinant dna in said methods

Publications (1)

Publication Number Publication Date
ATE275632T1 true ATE275632T1 (de) 2004-09-15

Family

ID=26139955

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96944694T ATE275632T1 (de) 1995-12-22 1996-12-23 Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar

Country Status (9)

Country Link
US (2) US6329141B1 (de)
EP (2) EP0870042B1 (de)
JP (1) JP2000507087A (de)
AT (1) ATE275632T1 (de)
AU (1) AU725340B2 (de)
CA (1) CA2241267C (de)
DE (1) DE69633343T2 (de)
ES (1) ES2229288T3 (de)
WO (1) WO1997023633A1 (de)

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JP3151371B2 (ja) * 1995-03-10 2001-04-03 麒麟麦酒株式会社 カロチノイド生産量の増量に有用なｄｎａ鎖
EP0870042B1 (de) * 1995-12-22 2004-09-08 DSM IP Assets B.V. Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
TWI250210B (en) 1998-05-06 2006-03-01 Dsm Ip Assets Bv An isolated DNA sequence coding for an enzyme involved in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate
EP0955363A3 (de) * 1998-05-06 2004-01-28 F. Hoffmann-La Roche Ag DNS Sequenzen kodierend für Enzyme für die Herstellung von Isoprenoiden
EP2305825B1 (de) 1998-07-06 2015-01-14 DCV Inc. doing business as Bio-Technical Resourses Verfahren zur Vitaminherstellung
CA2331343C (en) * 1998-07-06 2014-01-14 Dcv, Inc., Doing Business As Bio-Technical Resources Production of isoprenoid in recombinant yeast
KR100316741B1 (ko) * 1998-10-31 2002-09-04 한국생명공학연구원 파피아로도지마형질전환용벡터및그형질전환방법
US6365386B1 (en) * 1999-03-09 2002-04-02 Roche Vitamins Inc. Astaxanthin synthase
AUPQ262599A0 (en) * 1999-09-02 1999-09-23 University Of Queensland, The Gene expression
KR100371264B1 (ko) * 2000-01-25 2003-02-05 양문식 무라인 그래뉼로사이트-매크로파지 콜로니 자극 인자를포함하는 재조합 벡터와 그것을 발현하는 재조합아스퍼질러스 나이거
ES2254285T3 (es) * 2000-05-24 2006-06-16 Dsm Ip Assets B.V. Proceso para la produccion de astaxantina.
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US6818424B2 (en) 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
WO2002044357A2 (en) 2000-11-30 2002-06-06 Millennium Pharmaceuticals, Inc. Methods of using 18903 to treat pain and pain-related disorders
WO2005123915A1 (en) * 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
EA016258B1 (ru) 2005-03-18 2012-03-30 Микробиа, Инк. Рекомбинантные грибы, продуцирующие каротиноиды, и способы их применения
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
TW201632624A (zh) * 2011-11-28 2016-09-16 第一三共股份有限公司 源自人類基因的啟動子
JP2015226469A (ja) * 2012-09-24 2015-12-17 株式会社カネカ 新規ｄｎａ、およびそれを利用したカロテノイドの製造方法
CA3109156C (en) * 2014-05-16 2023-08-15 Academia Sinica Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
WO2020162959A1 (en) 2019-02-07 2020-08-13 The General Hospital Corporation Carotenoids for treating or preventing nausea

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
EP0164556B1 (de) 1984-05-11 1994-03-02 Chiron Corporation Erhöhte Hefetranskription unter Verwendung einer Hybridkonstruktion der Promotorregion
CA2047000A1 (en) * 1990-07-20 1992-01-21 John W. Chapman Astaxanthin-generating yeast cells
EP0509707A1 (de) 1991-04-08 1992-10-21 Petrella Limited Brotaufstrich mit einem Fettgehalt von 0 bis 40%
DK115191D0 (da) * 1991-06-14 1991-06-14 Ingrid Stampe Villadsen Fremgangsmaade til frembringelse af carotenoidproducerende, isaer astaxanthinproducerende, mikroorganismer, mikroorganismer opnaaet ved fremgangsmaaden og fremgangsmaade til fremstilling af carotenoidholdige mikroorganismeceller eller -celledele eller oprenset carotenoid
JPH05168465A (ja) * 1991-12-25 1993-07-02 Lion Corp ファフィア・ロドチーマの突然変異株およびその培養によるカロチノイド類の製造方法
NZ248628A (en) * 1992-09-11 1996-02-27 Gist Brocades Nv Transformed phaffia (yeast) strains and methods and vectors used
JP3151371B2 (ja) * 1995-03-10 2001-04-03 麒麟麦酒株式会社 カロチノイド生産量の増量に有用なｄｎａ鎖
EP0870042B1 (de) * 1995-12-22 2004-09-08 DSM IP Assets B.V. Verbesserte verfahren zur transformation von phaffiastämmen,transformierte phaffiastämme auf diese weise erhaltend, und rekominante dns in besagtem verfahren verwendbar

Also Published As

Publication number Publication date
EP1640456A2 (de) 2006-03-29
EP0870042A1 (de) 1998-10-14
JP2000507087A (ja) 2000-06-13
US6329141B1 (en) 2001-12-11
US20040091958A1 (en) 2004-05-13
WO1997023633A1 (en) 1997-07-03
CA2241267C (en) 2012-12-04
US7205123B2 (en) 2007-04-17
EP0870042B1 (de) 2004-09-08
CA2241267A1 (en) 1997-07-03
AU725340B2 (en) 2000-10-12
AU1308797A (en) 1997-07-17
ES2229288T3 (es) 2005-04-16
DE69633343T2 (de) 2005-09-22
EP1640456A3 (de) 2008-10-01
DE69633343D1 (de) 2004-10-14

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