DE69515028D1 - Die hocheffiziente herstellung und isolierung von viruspartikeln - Google Patents

Die hocheffiziente herstellung und isolierung von viruspartikeln

Info

Publication number
DE69515028D1
DE69515028D1 DE69515028T DE69515028T DE69515028D1 DE 69515028 D1 DE69515028 D1 DE 69515028D1 DE 69515028 T DE69515028 T DE 69515028T DE 69515028 T DE69515028 T DE 69515028T DE 69515028 D1 DE69515028 D1 DE 69515028D1
Authority
DE
Germany
Prior art keywords
exchange resin
insulation
cells
highly efficient
virus particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69515028T
Other languages
English (en)
Other versions
DE69515028T2 (de
Inventor
Christopher P Prior
David M Weber
Richard S Gore
James J Harter
Michael E Hrinda
Jonathan J Mitschelen
Thomas W Irish
Pierre M Bay
George C Tarr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immune Response Corp
Original Assignee
Immune Response Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immune Response Corp filed Critical Immune Response Corp
Publication of DE69515028D1 publication Critical patent/DE69515028D1/de
Application granted granted Critical
Publication of DE69515028T2 publication Critical patent/DE69515028T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16051Methods of production or purification of viral material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16111Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69515028T 1994-03-22 1995-03-22 Die hocheffiziente herstellung und isolierung von viruspartikeln Expired - Fee Related DE69515028T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21583394A 1994-03-22 1994-03-22
PCT/US1995/003668 WO1995025789A1 (en) 1994-03-22 1995-03-22 Highly efficient production and isolation of viral particles

Publications (2)

Publication Number Publication Date
DE69515028D1 true DE69515028D1 (de) 2000-03-16
DE69515028T2 DE69515028T2 (de) 2000-06-29

Family

ID=22804586

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69515028T Expired - Fee Related DE69515028T2 (de) 1994-03-22 1995-03-22 Die hocheffiziente herstellung und isolierung von viruspartikeln

Country Status (12)

Country Link
US (2) US5661023A (de)
EP (1) EP0751988B1 (de)
JP (1) JP3313117B2 (de)
AT (1) ATE189697T1 (de)
AU (1) AU2127395A (de)
CA (1) CA2186034C (de)
DE (1) DE69515028T2 (de)
DK (1) DK0751988T3 (de)
ES (1) ES2141929T3 (de)
GR (1) GR3033268T3 (de)
PT (1) PT751988E (de)
WO (1) WO1995025789A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2186034C (en) * 1994-03-22 2002-11-26 Michael E. Hrinda Highly efficient production and isolation of viral particles
FR2750433B1 (fr) * 1996-07-01 1998-08-14 Rhone Poulenc Rorer Sa Procede de production d'adenovirus recombinants
US6485958B2 (en) * 1996-07-01 2002-11-26 Gencell Sa Method for producing recombinant adenovirus
ATE348155T1 (de) 1996-11-20 2007-01-15 Introgen Therapeutics Inc Ein verbessertes verfahren zur produktion und reinigung von adenoviralen vektoren
US7732129B1 (en) 1998-12-01 2010-06-08 Crucell Holland B.V. Method for the production and purification of adenoviral vectors
JP2001514845A (ja) * 1997-09-05 2001-09-18 ターゲティッド ジェネティクス コーポレイション 組換えaavベクターの高力価ヘルパーなし調製物を生成するための方法
AU1956299A (en) * 1998-01-12 1999-07-26 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada A process for the isolation, recovery and purification of non-polar extractives
US6689600B1 (en) 1998-11-16 2004-02-10 Introgen Therapeutics, Inc. Formulation of adenovirus for gene therapy
DE10010342A1 (de) * 2000-03-06 2001-09-20 Merck Patent Gmbh Verfahren zur Abreicherung von Endotoxinen
SE0001132D0 (sv) * 2000-03-29 2000-03-29 Cavidi Tech Ab Method of concentrating and recovering a viral enzyme activity from biological samples
US20040101964A1 (en) * 2000-05-10 2004-05-27 Shigemitsu Takashima Method of preparing virus vector
US20020177571A1 (en) 2000-11-29 2002-11-28 Gordon Erlinda M. Targeted vectors for cancer immunotherapy
ATE425743T1 (de) * 2001-02-11 2009-04-15 Aquanova Ag Verfahren zur herstellung eines isoflavon- konzentrats
US20030031626A1 (en) * 2001-07-26 2003-02-13 Thomas Rheinlander Process for the production of pharmaceutical preparations
DE10137515A1 (de) * 2001-07-26 2003-02-13 Diagnostikforschung Inst Verfahren zur Herstellung pharmazeutischer Zubereitungen
US20070178066A1 (en) * 2003-04-21 2007-08-02 Hall Frederick L Pathotropic targeted gene delivery system for cancer and other disorders
US20090123428A1 (en) * 2003-04-21 2009-05-14 Hall Frederick L Pathotropic targeted gene delivery system for cancer and other disorders
EP2353389B1 (de) * 2003-04-21 2015-11-04 Epeius Biotechnologies Corporation Zusammensetzungen zur Behandlung von Erkrankungen
AU2005305347A1 (en) 2004-11-03 2006-05-18 Introgen Therapeutics Inc. Method of producing and purifying of adenoviral vectors
CN103993040B (zh) 2008-06-18 2018-02-13 牛津生物医学(英国)有限公司 病毒纯化
SG184833A1 (en) 2010-04-14 2012-11-29 Emd Millipore Corp Methods of producing high titer, high purity virus stocks and methods of use thereof
DE102010046817A1 (de) * 2010-09-28 2012-03-29 Sartorius Stedim Biotech Gmbh Verfahren zur Abtrennung von Viren aus einem Kontaminanten enthaltenden flüssigen Medium
CN103476399A (zh) 2011-04-18 2013-12-25 独立行政法人国立精神·神经医疗研究中心 药剂递送粒子及其制造方法
ES2868093T3 (es) 2012-09-17 2021-10-21 Grace W R & Co Medio y dispositivos de cromatografía
CN104768536B (zh) 2012-09-17 2019-03-29 格雷斯公司 官能化微粒状载体材料和其制备和使用方法
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
ES2896897T3 (es) 2015-06-05 2022-02-28 Grace W R & Co Agentes de clarificación para el bioprocesamiento de adsorbentes y métodos para producir y usar los mismos
WO2019104071A1 (en) 2017-11-22 2019-05-31 Corning Incorporated Perfusion bioreactor system and perfusion cell culture method
US11028124B2 (en) * 2018-05-07 2021-06-08 Repligen Corporation Methods, devices and systems for 3-stage filtration
WO2020214676A1 (en) * 2019-04-15 2020-10-22 Children's Hospital Medical Center Viral vector manufacturing methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3509070A (en) * 1968-06-13 1970-04-28 American Home Prod Adsorbent for chromatographic purification of virus
US3655509A (en) * 1969-05-29 1972-04-11 Monsanto Co Process for the separation of virus from non-viral proteins
US3925152A (en) * 1971-06-18 1975-12-09 Exploaterings Ab Tbf Virus separation
US4647773A (en) * 1984-04-23 1987-03-03 The United States Of America As Represented By The Department Of Health And Human Services Method of continuous production of retroviruses (HTLV-III) from patients with AIDS and pre-AIDS
JPS6147185A (ja) * 1984-08-09 1986-03-07 Chemo Sero Therapeut Res Inst 日本脳炎ウイルスの精製方法
JPS6151571A (ja) * 1984-08-21 1986-03-14 Nippon Seibutsu Kagaku Kenkyusho オ−エスキ−病診断用抗原の単離・精製法
ATE132374T1 (de) * 1987-06-10 1996-01-15 Immune Response Corp Inc Verhütung und behandlung einer retrovirenkrankheit
NZ225583A (en) * 1987-08-06 1991-07-26 Merck & Co Inc Process for purifying hepatitis a virions (hav)
EP0519001B2 (de) * 1990-03-09 2007-12-19 Novartis Vaccines and Diagnostics, Inc. Gereinigtes gp120, in dem seine natürliche konformation erhalten bleibt
FR2661834B1 (fr) * 1990-05-09 1995-01-27 Merieux Inst Procede de fabrication d'immunogenes de retrovirus et de vaccins contre les infections retrovirales, notamment hiv, et immunogenes et vaccins obtenus.
AU650711B2 (en) * 1990-12-13 1994-06-30 Cellco, Inc. Sustained and continuous production of high titers of recombinant viral vectors and transduced target cells for use in gene therapy
CA2186034C (en) * 1994-03-22 2002-11-26 Michael E. Hrinda Highly efficient production and isolation of viral particles
JPH08195877A (ja) * 1995-01-18 1996-07-30 Canon Inc イメージセンサと画像処理回路

Also Published As

Publication number Publication date
EP0751988B1 (de) 2000-02-09
CA2186034A1 (en) 1995-09-28
US5661023A (en) 1997-08-26
EP0751988A4 (de) 1997-11-12
PT751988E (pt) 2000-05-31
CA2186034C (en) 2002-11-26
DK0751988T3 (da) 2000-06-26
JPH10500286A (ja) 1998-01-13
AU2127395A (en) 1995-10-09
US6080571A (en) 2000-06-27
JP3313117B2 (ja) 2002-08-12
ES2141929T3 (es) 2000-04-01
ATE189697T1 (de) 2000-02-15
GR3033268T3 (en) 2000-09-29
DE69515028T2 (de) 2000-06-29
WO1995025789A1 (en) 1995-09-28
EP0751988A1 (de) 1997-01-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee