DE69513751D1 - Reinigung von Alpha-l-proteinaseinhibitor mittels neuen chromatographischen Trennungsbedingungen - Google Patents

Reinigung von Alpha-l-proteinaseinhibitor mittels neuen chromatographischen Trennungsbedingungen

Info

Publication number
DE69513751D1
DE69513751D1 DE69513751T DE69513751T DE69513751D1 DE 69513751 D1 DE69513751 D1 DE 69513751D1 DE 69513751 T DE69513751 T DE 69513751T DE 69513751 T DE69513751 T DE 69513751T DE 69513751 D1 DE69513751 D1 DE 69513751D1
Authority
DE
Germany
Prior art keywords
alpha
proteinase inhibitor
purification
chromatographic separation
cation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69513751T
Other languages
English (en)
Other versions
DE69513751T2 (de
Inventor
Wytold R Lebing
Sharon X Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of DE69513751D1 publication Critical patent/DE69513751D1/de
Publication of DE69513751T2 publication Critical patent/DE69513751T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Analytical Chemistry (AREA)
  • Pulmonology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69513751T 1994-08-24 1995-08-11 Reinigung von Alpha-l-proteinaseinhibitor mittels neuen chromatographischen Trennungsbedingungen Expired - Lifetime DE69513751T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/295,119 US5610285A (en) 1994-08-24 1994-08-24 Purification of α-1 proteinase inhibitor using novel chromatographic separation conditions

Publications (2)

Publication Number Publication Date
DE69513751D1 true DE69513751D1 (de) 2000-01-13
DE69513751T2 DE69513751T2 (de) 2000-04-27

Family

ID=23136291

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69513751T Expired - Lifetime DE69513751T2 (de) 1994-08-24 1995-08-11 Reinigung von Alpha-l-proteinaseinhibitor mittels neuen chromatographischen Trennungsbedingungen

Country Status (13)

Country Link
US (1) US5610285A (de)
EP (1) EP0698615B1 (de)
JP (1) JP3770414B2 (de)
KR (1) KR100451266B1 (de)
CN (2) CN1161371C (de)
AT (1) ATE187462T1 (de)
CA (2) CA2156007C (de)
DE (1) DE69513751T2 (de)
DK (1) DK0698615T3 (de)
ES (1) ES2139794T3 (de)
GR (1) GR3032728T3 (de)
HK (1) HK1072372A1 (de)
PT (1) PT698615E (de)

Families Citing this family (48)

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AU6884396A (en) * 1995-09-07 1997-03-27 Ppl Therapeutics (Scotland) Ltd Purification of alpha-1 proteinase inhibitor
SE9600796D0 (sv) * 1996-02-29 1996-02-29 Pharmacia Biotech Ab A method of preparing a liquid mixture
US5616693A (en) * 1996-07-01 1997-04-01 Alpha Therapeutic Corporation Process for seperating alpha-1-proteinase inhibitor from COHN IV1 +1V4 paste
GB2318732A (en) 1996-11-01 1998-05-06 Johnson & Johnson Medical Wound healing compositions containing alpha-1-antitrypsin
AT407114B (de) * 1997-06-10 2000-12-27 Immuno Ag Alpha 1-antitrypsin-präparation sowie verfahren zu deren herstellung
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
US6093804A (en) * 1998-09-24 2000-07-25 American National Red Cross Method for purification of alpha-1 proteinase inhibitor
AUPP790698A0 (en) * 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
US6489308B1 (en) 1999-03-05 2002-12-03 Trustees Of University Of Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of nitric-oxide-induced clinical conditions
WO2000051625A1 (en) * 1999-03-05 2000-09-08 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of herpes viruses
US6849605B1 (en) * 1999-03-05 2005-02-01 The Trustees Of University Technology Corporation Inhibitors of serine protease activity, methods and compositions for treatment of viral infections
AU3731400A (en) 1999-03-05 2000-09-21 Trustees Of University Technology Corporation, The Methods and compositions useful in inhibiting apoptosis
AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6462180B1 (en) * 1999-11-24 2002-10-08 Bayer Corporation Method of preparing α-1 proteinase inhibitor
US7077942B1 (en) 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
AUPQ691400A0 (en) * 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
ATE449200T1 (de) 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
CN1235667C (zh) * 2000-10-06 2006-01-11 格拉迪普有限公司 多口分离装置及方法
NZ526940A (en) 2000-12-14 2005-06-24 Bayer Healthcare Llc Method of preparing alpha-1 proteinase inhibitor
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
US7777006B2 (en) 2002-12-31 2010-08-17 Csl Behring L.L.C. Method for purification of alpha-1-antitrypsin
US20040220242A1 (en) * 2003-05-02 2004-11-04 Leland Shapiro Inhibitors of serine protease activity, methods and compositions for treatment of nitric oxide induced clinical conditions
PL2520654T3 (pl) 2003-08-26 2017-08-31 The Regents Of The University Of Colorado, A Body Corporate Inhibitory aktywności proteazy serynowej i ich zastosowanie w sposobach i kompozycjach do leczenia zakażeń bakteryjnych
SI1664123T2 (sl) * 2003-09-22 2012-03-30 Kamada Ltd Priprava inhibitorja alfa proteinaze v velikem merilu in njegova uporaba
CN1314447C (zh) * 2004-04-12 2007-05-09 爱华生物科技(香港)有限公司 一种具有抑制蛋白酶作用的药物的制备方法
CA2654446C (en) * 2005-06-07 2014-02-25 The Regents Of The University Of Colorado Inhibitors of serine protease activity and their use in methods and compositions for treatment of graft rejection and promotion of graft survival
US7807435B2 (en) * 2005-08-11 2010-10-05 Baxter International Inc. Method for the purification of alpha-1 proteinase inhibitor (a1PI)
US20080124303A1 (en) * 2005-12-12 2008-05-29 Cavit Sciences, Inc Methods and compositions for treatment of viral infections
US8623830B2 (en) 2006-09-12 2014-01-07 Beth Israel Deaconess Medical Center, Inc. Compositions containing α-1-antitrypsin and methods for use
MX2009012786A (es) * 2007-06-01 2009-12-10 Hoffmann La Roche Purificacion de inmunoglobulina.
PL2183268T3 (pl) 2007-08-17 2013-03-29 Csl Behring Gmbh Sposoby oczyszczania alfa-1-antytrypsyny i apolipoproteiny A-I
CN102099369A (zh) * 2008-07-18 2011-06-15 泰勒克里斯生物治疗学公司 制备α-1蛋白酶抑制剂的方法
KR101104637B1 (ko) * 2010-02-26 2012-01-12 한국철도기술연구원 무개화차의 컨테이너 고정장치
WO2011150284A2 (en) 2010-05-26 2011-12-01 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
AU2010202125B1 (en) 2010-05-26 2010-09-02 Takeda Pharmaceutical Company Limited A method to produce an immunoglobulin preparation with improved yield
CA2839917A1 (en) 2011-06-24 2012-12-27 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and uses for alpha-1 antitrypsin fusion molecules
US9421248B2 (en) 2011-12-30 2016-08-23 Grifols, S.A. Alpha 1-proteinase inhibitor for delaying the onset or progression of pulmonary exacerbations
KR20140137347A (ko) 2012-01-10 2014-12-02 더 리젠츠 오브 더 유니버시티 오브 콜로라도, 어 바디 코포레이트 알파-1 안티트립신 융합 분자용 조성물, 방법 및 용도
SG11201506775VA (en) 2013-03-08 2015-09-29 Genzyme Corp Continuous purification of therapeutic proteins
EP3708182A1 (de) 2013-03-29 2020-09-16 The Regents Of The University Of Colorado Zusammensetzungen und verfahren zur vorbereitung eines patienten auf ein organisches oder nichtorganisches implantat
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
LT3215528T (lt) 2014-11-06 2019-10-25 Hoffmann La Roche Fc srities variantai su modifikuota fcrn jungtimi ir naudojimo būdai
AU2019332764A1 (en) 2018-08-31 2021-05-06 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1189674B (de) * 1963-06-12 1965-03-25 Behringwerke Ag Verfahren zur Isolierung von alpha-Antitrypsin
US4379087A (en) * 1982-06-17 1983-04-05 Cutter Laboratories, Inc. Method of preparing alpha-1-proteinase inhibitor
US4752576A (en) * 1984-06-14 1988-06-21 Chiron Corporation Expression of α-1 antitrypsin in yeast
US4656254A (en) * 1985-12-02 1987-04-07 Miles Laboratories, Inc. Method of preparing alpha-1-proteinase inhibitor and antithrombin III
US4629567A (en) * 1986-03-07 1986-12-16 Smithkline-Rit Alpha-1-antiprotease purification
US4749783A (en) 1986-07-11 1988-06-07 Miles Laboratories, Inc. Viral inactivation and purification of active proteins
FR2610633B1 (fr) * 1987-02-05 1992-09-18 Lille Transfusion Sanguine Procede d'obtention d'un concentre d'a 1-antitrypsine a partir de plasma humain et son utilisation a titre de medicament
US5319072A (en) * 1992-01-10 1994-06-07 Alpha Therapeutic Corporation Human antithrombin-III preparation

Also Published As

Publication number Publication date
AU700638B2 (en) 1999-01-07
HK1072372A1 (en) 2005-08-26
ES2139794T3 (es) 2000-02-16
CA2156007A1 (en) 1996-02-25
US5610285A (en) 1997-03-11
DE69513751T2 (de) 2000-04-27
KR100451266B1 (ko) 2004-12-23
ATE187462T1 (de) 1999-12-15
PT698615E (pt) 2000-05-31
KR960007617A (ko) 1996-03-22
JPH08176198A (ja) 1996-07-09
GR3032728T3 (en) 2000-06-30
AU3011195A (en) 1996-03-07
CN1125231A (zh) 1996-06-26
CA2692337A1 (en) 1996-02-25
EP0698615B1 (de) 1999-12-08
JP3770414B2 (ja) 2006-04-26
EP0698615A1 (de) 1996-02-28
DK0698615T3 (da) 2000-05-15
CA2156007C (en) 2010-03-09
CN1572321A (zh) 2005-02-02
CN100548372C (zh) 2009-10-14
CN1161371C (zh) 2004-08-11

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