ES2070855T3 - Metodo para purificar una proteina. - Google Patents
Metodo para purificar una proteina.Info
- Publication number
- ES2070855T3 ES2070855T3 ES88309824T ES88309824T ES2070855T3 ES 2070855 T3 ES2070855 T3 ES 2070855T3 ES 88309824 T ES88309824 T ES 88309824T ES 88309824 T ES88309824 T ES 88309824T ES 2070855 T3 ES2070855 T3 ES 2070855T3
- Authority
- ES
- Spain
- Prior art keywords
- disclosed
- protein
- purify
- technique
- impurities
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/26—Cation exchangers for chromatographic processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
TECNICA DE CROMATOGRAFIA DE INTERCAMBIO IONICO PARA PURIFICAR PROTEINAS CRUZADAS, QUE CONTIENEN INPUREZAS MUY SIMILARES, EN LA QUE SE DETERMINAN LOS PUNTOS ISOELECTRICOS DE LA PROTEINA DESEADA Y DE LAS INPUREZAS. TAMBIEN SE INCLUYE LA APLICACION DE LA TECNICA A LA PURIFICACION DE GM - CSF USANDO UNA RSINA CAMBIADORA DE CATIONES.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11188687A | 1987-10-23 | 1987-10-23 | |
US111886 | 1987-10-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2070855T3 true ES2070855T3 (es) | 1995-06-16 |
ES2070855T5 ES2070855T5 (es) | 2004-03-01 |
Family
ID=22340970
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88309824T Expired - Lifetime ES2070855T5 (es) | 1987-10-23 | 1988-10-19 | Metodo para purificar una proteina. |
Country Status (21)
Country | Link |
---|---|
EP (2) | EP0313343B2 (es) |
JP (1) | JPH0788395B2 (es) |
KR (1) | KR930008447B1 (es) |
CN (1) | CN1031943C (es) |
AT (1) | ATE121418T1 (es) |
AU (1) | AU626008B2 (es) |
DE (1) | DE3853610T3 (es) |
DK (1) | DK99690D0 (es) |
ES (1) | ES2070855T5 (es) |
FI (1) | FI103974B1 (es) |
HU (1) | HU204537B (es) |
IE (1) | IE72219B1 (es) |
IL (1) | IL88118A (es) |
MY (1) | MY108512A (es) |
NO (1) | NO303451B1 (es) |
NZ (1) | NZ226651A (es) |
OA (1) | OA09787A (es) |
PT (1) | PT88813B (es) |
TN (1) | TNSN88111A1 (es) |
WO (1) | WO1989003840A1 (es) |
ZA (1) | ZA887862B (es) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE68929566D1 (de) * | 1988-05-13 | 2010-01-28 | Amgen Inc | Verfahren zur Isolierung und Reinigung von G-CSF |
US7217689B1 (en) | 1989-10-13 | 2007-05-15 | Amgen Inc. | Glycosylation analogs of erythropoietin |
US5110913A (en) * | 1990-05-25 | 1992-05-05 | Miles Inc. | Antibody purification method |
US5463029A (en) * | 1992-11-23 | 1995-10-31 | Immunex Corporation | Purification of fusion proteins comprising GM-CSF and IL-3 |
JPH09502728A (ja) * | 1993-09-15 | 1997-03-18 | アルファ セラピューティク コーポレイション | α▲下1▼−酸性糖タンパク質の精製方法及び精製物 |
US5808011A (en) * | 1996-07-01 | 1998-09-15 | Biopure Corporation | Method for chromatographic removal of prions |
US7304150B1 (en) | 1998-10-23 | 2007-12-04 | Amgen Inc. | Methods and compositions for the prevention and treatment of anemia |
ES2208305T3 (es) | 1999-04-08 | 2004-06-16 | Genentech, Inc. | Composicion basada en polipeptidos de carga opuesta.. |
ES2626268T3 (es) * | 2002-09-11 | 2017-07-24 | Chugai Seiyaku Kabushiki Kaisha | Método de purificación de proteínas |
DE10360841A1 (de) * | 2003-12-20 | 2005-07-14 | Henkel Kgaa | Helle, stabile, staub- und geruchsarme Enzymgranulate |
KR101660575B1 (ko) * | 2005-03-11 | 2016-09-27 | 와이어쓰 엘엘씨 | 약한 분배성 크로마토그래피법 |
SE529259C2 (sv) * | 2005-08-31 | 2007-06-12 | Ge Healthcare Bio Sciences Ab | Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi |
AU2007317200B8 (en) * | 2006-11-10 | 2012-02-02 | Agriculture Victoria Services Pty Ltd. | Process for the preparation of angiogenin |
CN101556261B (zh) * | 2009-05-25 | 2012-01-11 | 北京理工大学 | 微管内等电聚焦和剪断吹出培养测定微生物等电点的方法 |
CN101556260B (zh) * | 2009-05-25 | 2012-01-11 | 北京理工大学 | 固定化pH梯度毛细管等电聚焦测定微生物等电点的方法 |
WO2013075740A1 (en) * | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
TR201815709T4 (tr) * | 2011-12-22 | 2018-11-21 | Hoffmann La Roche | İyon değişim membranı kromatografisi. |
EP2994485B1 (en) | 2013-05-06 | 2018-01-10 | Sanofi | Continuous multistep process for purifying antibodies |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4658018A (en) * | 1984-03-13 | 1987-04-14 | Immunex Corporation | Process for producing homogeneous colony stimulating factor |
IL76360A0 (en) * | 1984-09-26 | 1986-01-31 | Takeda Chemical Industries Ltd | Mutual separation of proteins |
ZA872781B (en) * | 1986-05-19 | 1987-10-05 | Immunology Ventures | B-cell stimulating factor |
-
1988
- 1988-10-19 AT AT88309824T patent/ATE121418T1/de not_active IP Right Cessation
- 1988-10-19 HU HU886264A patent/HU204537B/hu not_active IP Right Cessation
- 1988-10-19 JP JP63508950A patent/JPH0788395B2/ja not_active Expired - Lifetime
- 1988-10-19 EP EP88309824A patent/EP0313343B2/en not_active Expired - Lifetime
- 1988-10-19 DE DE3853610T patent/DE3853610T3/de not_active Expired - Fee Related
- 1988-10-19 KR KR1019890701131A patent/KR930008447B1/ko not_active IP Right Cessation
- 1988-10-19 ES ES88309824T patent/ES2070855T5/es not_active Expired - Lifetime
- 1988-10-19 AU AU26247/88A patent/AU626008B2/en not_active Ceased
- 1988-10-19 WO PCT/US1988/003589 patent/WO1989003840A1/en active IP Right Grant
- 1988-10-19 EP EP88909648A patent/EP0391934A1/en active Pending
- 1988-10-20 TN TNTNSN88111A patent/TNSN88111A1/fr unknown
- 1988-10-20 NZ NZ226651A patent/NZ226651A/xx unknown
- 1988-10-20 MY MYPI88001190A patent/MY108512A/en unknown
- 1988-10-20 ZA ZA887862A patent/ZA887862B/xx unknown
- 1988-10-20 PT PT88813A patent/PT88813B/pt not_active IP Right Cessation
- 1988-10-20 IE IE316888A patent/IE72219B1/en not_active IP Right Cessation
- 1988-10-21 IL IL88118A patent/IL88118A/xx not_active IP Right Cessation
- 1988-10-21 CN CN88107266A patent/CN1031943C/zh not_active Expired - Fee Related
-
1990
- 1990-04-17 OA OA59777A patent/OA09787A/en unknown
- 1990-04-20 NO NO901769A patent/NO303451B1/no not_active IP Right Cessation
- 1990-04-23 DK DK099690A patent/DK99690D0/da unknown
- 1990-04-23 FI FI902017A patent/FI103974B1/fi not_active IP Right Cessation
Also Published As
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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