KR960004367A - α1-안티카이모트립신의 제조방법 - Google Patents

α1-안티카이모트립신의 제조방법 Download PDF

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KR960004367A
KR960004367A KR1019950022667A KR19950022667A KR960004367A KR 960004367 A KR960004367 A KR 960004367A KR 1019950022667 A KR1019950022667 A KR 1019950022667A KR 19950022667 A KR19950022667 A KR 19950022667A KR 960004367 A KR960004367 A KR 960004367A
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act
iii
conductivity
antichymotrypsin
contacting
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KR1019950022667A
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KR100433115B1 (ko
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씨. 쎄이 그레이스
케이. 에이치. 청 닐
디. 베튼코오트 제프리
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제임스 에이. 깁린
바이엘 코포레이션
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
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  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Communicable Diseases (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

본 발명은 크로마토그래피 흡착 단계를 사용하여 조심스럽게 조절된 pH와 전도도에서 사람 α1-프로테이나제 억제제(PI) 및 안티트롬빈 Ⅲ-(AT-Ⅲ)을 함유하는 용액으로 부터 사람 α1-안티카이모트립신(ACT)을 정제하는 방법에 관한 것이다. 분리된 ACT는 시험관내 억제능을 가지며 강력한 치료적 용도를 갖는다.

Description

α1-안티카이모트립신의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 사람혈장으로부터 바람직한 출발물질(콜 분획 Ⅳ-1페이스트현탁액)을 어떻게 분획화 시키는지를 나타내는, 일반화시킨 흐름도이다.
제2도는 ACT정제 과정의 바람직한 단계를 나타내는 흐름도이다.

Claims (9)

  1. (A)실질적으로 모든 α1-안티카이모트립신(ACT)을 흡착시키는 pH 및 전도도의 조건하에, α1-안티카이모트트립신, α1-프로테이나제 억제제(PI) 및 안티-트롬빈 Ⅲ-(AT-Ⅲ)의 혼합물의 수성 현탁액을 흡착제와 접촉시키는 단계; (B)실질적으로 모든 PI 및 AT-Ⅲ를 용출시키기 충분한 pH 및 전도도의 조건하에 흡착제를 세척시키는 단계 및 (C)흡착제로부터 ACT를 용출시키는 단계를 포함하여, 상기 α1-안티카이모트립신, α1-프로테이나제 억제제 및 안티-트롬빈 Ⅲ의 혼합물로부터 α1-안티카이모트립신을 분리시키는 방법.
  2. 제1항에 있어서, ACT를 흡착시키기에 충분한 조건하에 단계 (C)의 용출 생성물을 DNA-셀룰로즈와 접촉시키고 DNA-셀룰로즈부터 ACT를 용출시키기에 충분한 조건하에서 용출시킴을 포함하는 방법.
  3. (A)ACT, PI 및 AT-Ⅲ을 포함하는 수용액을 수득하는 단계; (B)이 용액을, ACT, PI 및 AT-Ⅲ을 흡착시키기 충분한 전도도 및 pH에서 이온 교환 수지와 접촉시키는 단계; (C)실질적으로 모든 PI 및 AT-Ⅲ를 용출시키기 충분한 pH 및 전도도의 조건하에서 흡착제를 세척하는 단계; (D)수지로부터 ACT를 용출시켜 용출액을 형성시키는 단계; (E)상기 용출액을, ACT를 흡착시키기 충분한 pH 및 조건하에서 DNA-셀룰로즈와 접촉시키는 단계 및 (F)DNA-셀룰로즈로부터 ACT를 용출시키는 단계를 포함하여, 사람의 ACT 및 사람의 α1-프로테이나제 억제제(PI) 및 사람의 안티-트롬빈 Ⅲ-(AT-Ⅲ)의 혼합물로부터 사람의 α1-안티카이모트립신(ACT)를 제조하는 방법.
  4. 제3항에 있어서, 단계(A)의 용액이 콘 분획 Ⅳ-1 페이스트 현탁액인 방법.
  5. 제3항에 있어서, 단계(B)의 이온 교환 수지가 음이온 교환 수지이고 용액의 pH가 약 6.5이며 전도도가 약 1.0 내지 2.5㎜ho/㎝인 방법.
  6. 제3항에 있어서, 단계(C)의 세척을, pH 약 6.5 및 전도도 7.0 내지 7.8㎜ho/㎝인 수용액으로 수행하는 방법.
  7. 제3항에 있어서, 단계(E)의 접촉을, pH가 약 6.8이고 전도도가 1.0 내지 2.0㎜ho/㎝인 용액을 사용하여 수행하는 방법.
  8. 순도가 90% 이상이고, 델마르 등의 어널리시스 바이오켐[DelMar et al, Anal, Biochem, 99:316(1979)]에 기술된 카텝신 지(Cathepsin G)분석법을 사용한 활성이 1.0㎎/총 단백질 ㎎을 초과하는 고도로 정제된 α1-안티카이모트립신 생성물.
  9. 제 8항에 있어서 약제학적으로 허용되는 담체중에 존재하는 생성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950022667A 1994-07-29 1995-07-28 α1-안티카이모트립신의제조방법 KR100433115B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/282,860 1994-07-29
US08/282860 1994-07-29
US08/282,860 US5561108A (en) 1994-07-29 1994-07-29 Preparation of α1 -antichymotrypsin

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KR100433115B1 KR100433115B1 (ko) 2004-08-30

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US (1) US5561108A (ko)
EP (1) EP0694562B1 (ko)
JP (1) JP3693391B2 (ko)
KR (1) KR100433115B1 (ko)
CN (1) CN1175102C (ko)
AT (1) ATE197461T1 (ko)
CA (1) CA2154982C (ko)
DE (1) DE69519338T2 (ko)
DK (1) DK0694562T3 (ko)
ES (1) ES2151570T3 (ko)
GR (1) GR3035340T3 (ko)
PT (1) PT694562E (ko)

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AUPP751198A0 (en) * 1998-12-04 1999-01-07 Csl Limited Purification of A1-proteinase inhibitor
US20050265977A1 (en) * 1999-04-30 2005-12-01 Elliott Robert B Xenotransplant for CNS therapy
US20090047325A1 (en) * 1999-04-30 2009-02-19 Neurotrophincell Pty. Limited Xenotransplant for cns therapy
AU4440000A (en) * 1999-04-30 2000-11-17 Diatranz Limited Xenotransplant for cns therapy
EP1248640B1 (en) * 2000-01-20 2006-10-04 Diabcell Pty Limited Preparation and xenotransplantation of porcine islets
AU1112202A (en) * 2000-10-17 2002-04-29 Diatranz Ltd Preparation and xenotransplantation or porcine islets
NZ515310A (en) * 2001-11-07 2004-08-27 Diabcell Pty Ltd Methods of treatment and delivery modes
EP1636346A4 (en) * 2003-06-24 2007-03-28 Diabcell Pty Ltd FROM PIG CULTIVATED PIG ISLE CELLS FOR XENOTRANSPLANTATION
NZ540597A (en) * 2005-06-08 2007-02-23 Neurotrophincell Pty Ltd A method for preventing the onset of type I diabetes comprising administering an implantable composition comprising living choroid plexus cells
CN103160486A (zh) * 2013-04-02 2013-06-19 黑龙江迪龙制药有限公司 一种猪凝血酶的制备方法
CN110964707A (zh) * 2019-12-31 2020-04-07 江西浩然生物制药有限公司 一种糜蛋白酶的提取纯化方法
CN112062833A (zh) * 2020-10-09 2020-12-11 国药集团武汉血液制品有限公司 一种从血浆组分ⅳ沉淀中提取人血白蛋白的方法

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DE3271827D1 (en) * 1981-05-01 1986-07-31 Medical Research Inst Of San F Method for isolating alpha-1-antitrypsin
US4379087A (en) * 1982-06-17 1983-04-05 Cutter Laboratories, Inc. Method of preparing alpha-1-proteinase inhibitor
US5079336A (en) * 1989-06-23 1992-01-07 The Trustees Of The University Of Pennsylvania α-1-antichymotrypsin, analogues and methods of production

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AU2714395A (en) 1996-02-08
JP3693391B2 (ja) 2005-09-07
EP0694562B1 (en) 2000-11-08
AU700921B2 (en) 1999-01-14
KR100433115B1 (ko) 2004-08-30
PT694562E (pt) 2001-04-30
CA2154982C (en) 2008-03-18
DE69519338D1 (de) 2000-12-14
US5561108A (en) 1996-10-01
DK0694562T3 (da) 2001-01-02
CN1175102C (zh) 2004-11-10
CA2154982A1 (en) 1996-01-30
DE69519338T2 (de) 2001-05-31
ATE197461T1 (de) 2000-11-11
CN1133885A (zh) 1996-10-23
GR3035340T3 (en) 2001-05-31
JPH08176197A (ja) 1996-07-09
EP0694562A1 (en) 1996-01-31
ES2151570T3 (es) 2001-01-01

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