KR900004933A - 조직 플라스미노겐 액티베이터의 정제방법 - Google Patents
조직 플라스미노겐 액티베이터의 정제방법 Download PDFInfo
- Publication number
- KR900004933A KR900004933A KR1019890012820A KR890012820A KR900004933A KR 900004933 A KR900004933 A KR 900004933A KR 1019890012820 A KR1019890012820 A KR 1019890012820A KR 890012820 A KR890012820 A KR 890012820A KR 900004933 A KR900004933 A KR 900004933A
- Authority
- KR
- South Korea
- Prior art keywords
- tpa
- cation
- cation exchanger
- exchanger
- purification method
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/815—Enzyme separation or purification by sorption
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (2)
- (a)tPA 및 불필요한 단백질을 함유한 조 tPA제제를 양이온 교환기와 접촉시켜 tPA 및 불필요한 단백질을 양이온 교환기에 흡착시키고, (b)상기 양이온 교환기를 pH5.2∼6.2의 용리액으로 처리하여 tPA와 같거나 그 이하의 pI 값을 갖는 불필요한 단백질을 용출시키고, (c) (b) 단계를 거친 양이온 교환기를 pH2.8∼3.5의 용리액으로 처리하여 tPA와 같거나 그 이상의 pI 값을 갖는 불필요한 단백질을 용출시키고 (d) (b) 및 (c) 단계를 거친 양이온 교환기로부터 tPA를 용출시키는 단계를 특징으로 하는 tPA의 정제방법.
- 제1항에 있어서 양이온 교환기(cation-exchanger)가 양이온 교환기(cation-exchange group)로서 카르복시메틸기를 갖는 tPA의 정제방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP63221843A JPH0824578B2 (ja) | 1988-09-05 | 1988-09-05 | tPAの精製方法 |
JP221843 | 1988-09-05 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR900004933A true KR900004933A (ko) | 1990-04-13 |
KR910006600B1 KR910006600B1 (ko) | 1991-08-29 |
Family
ID=16773062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890012820A KR910006600B1 (ko) | 1988-09-05 | 1989-09-05 | 조직 플라스미노겐 액티베이터의 정제방법 |
Country Status (6)
Country | Link |
---|---|
US (1) | US5158882A (ko) |
EP (1) | EP0358274B1 (ko) |
JP (1) | JPH0824578B2 (ko) |
KR (1) | KR910006600B1 (ko) |
CA (1) | CA1335186C (ko) |
DE (1) | DE68915230T2 (ko) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090130714A1 (en) * | 2007-09-24 | 2009-05-21 | Reliance Life Sciences Pvt.Ltd. | Process for purifying recombinanat tissue plasminogen activator (TPA) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS53107479A (en) * | 1977-02-28 | 1978-09-19 | Green Cross Corp:The | Preparation of human lysozyme |
US4264738A (en) * | 1979-08-01 | 1981-04-28 | Stepanov Valentin M | Process for purification of proteolytic enzymes |
US4374926A (en) * | 1981-05-13 | 1983-02-22 | Smith Laboratories, Inc. | Method for the production of improved chymopapain |
JPS60174729A (ja) * | 1984-02-21 | 1985-09-09 | Nippon Shinyaku Co Ltd | 抗癌医薬組成物 |
DE3439980A1 (de) * | 1984-11-02 | 1986-05-07 | Behringwerke Ag, 3550 Marburg | Verfahren zur reinigung sowie pasteurisierung von urokinase |
JPS61246131A (ja) * | 1985-04-22 | 1986-11-01 | 雪印乳業株式会社 | 新しいタイプのプラスミノ−ゲン活性化因子及びその調製方法 |
JPS6379591A (ja) * | 1986-09-22 | 1988-04-09 | Mitsui Toatsu Chem Inc | tPAの精製方法 |
-
1988
- 1988-09-05 JP JP63221843A patent/JPH0824578B2/ja not_active Expired - Lifetime
-
1989
- 1989-08-31 CA CA000609971A patent/CA1335186C/en not_active Expired - Fee Related
- 1989-09-05 EP EP89202239A patent/EP0358274B1/en not_active Expired - Lifetime
- 1989-09-05 DE DE68915230T patent/DE68915230T2/de not_active Expired - Lifetime
- 1989-09-05 KR KR1019890012820A patent/KR910006600B1/ko not_active IP Right Cessation
-
1992
- 1992-02-28 US US07/842,759 patent/US5158882A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA1335186C (en) | 1995-04-11 |
JPH0824578B2 (ja) | 1996-03-13 |
KR910006600B1 (ko) | 1991-08-29 |
DE68915230D1 (de) | 1994-06-16 |
US5158882A (en) | 1992-10-27 |
EP0358274B1 (en) | 1994-05-11 |
EP0358274A2 (en) | 1990-03-14 |
EP0358274A3 (en) | 1990-09-26 |
JPH0269184A (ja) | 1990-03-08 |
DE68915230T2 (de) | 1994-08-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR900701823A (ko) | 혈장단백질의 크로마토그래피 분리법 | |
HK1072372A1 (en) | Alpha-1 proteinase inhibitor preparation | |
SUEYOSHI et al. | Purification and characterization of four isolectins of mushroom (Agaricus bisporus) | |
FI943368A (fi) | Mentelmä laktoferriinin ja laktoperoksidaasin eristämiseksi maidosta ja maitotuotteista ja menetelmällä saadut tuotteet | |
MX9707909A (es) | Purificacion de anticuerpos. | |
ES2106742T3 (es) | Procedimiento de purificacion de proteinas. | |
DE3672973D1 (de) | Verfahren zur spezifischen abtrennung von lactose aus milch. | |
US4168261A (en) | Method for the purification of interferon using porous glass beads | |
EP0385118A3 (en) | Use of compounds containing or binding sialic acid to neutralize bacterial toxins | |
Baecker et al. | Purification of serine acetyltransferase, a component of a multienzyme complex, by immunoadsorption and selective dissociation of the complex | |
KR900004933A (ko) | 조직 플라스미노겐 액티베이터의 정제방법 | |
KR960705921A (ko) | 사람 활성화 단백질 C제제 및 이의 제조 방법(Human activated protein C preparation and process for producing the same) | |
RU95112503A (ru) | Способ выделения предварительно отобранного гемоглобина и очищенный препарат гемоглобина | |
KR900015748A (ko) | 안티트롬빈-ⅲ의 정제 방법 및 이 안티트롭빈-ⅲ를 함유한 안티트롬빈-ⅲ 제제 | |
Cole et al. | Phosphocellulose, an affinity chromatographic system for chorismate synthase and the aromatic complex of Neurospora crassa | |
US4677194A (en) | Isolation of an endotoxin inactivator from human plasma | |
KR910004208A (ko) | 안정성인자 viii의 제조방법 | |
Comis et al. | Stabilization of lethal and hemolytic activities of box jellyfish (Chironex fleckeri) venom | |
KR910015699A (ko) | 리포코틴의 정제방법 | |
TAKAHASHI | STUDIES ON SULFATASES OF THE LIVER OF CHARONIA LAMPAS II. PURIFICATION AND PROPERTIES OF SULFATASES | |
KR820002452A (ko) | 항생물질 네오스라마이신(neothramycin)의 정제방법 | |
JPS57135356A (en) | Analysis of hemoglobin | |
Shpanchenko et al. | Isolation, purification and properties of cellobiohydrolase from Trichoderma longibrachiatum. | |
RU1839190C (ru) | Способ очистки микробной эстеразы | |
KR920019945A (ko) | 란티바이오틱의 분리 및 정제방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
G160 | Decision to publish patent application | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 19970819 Year of fee payment: 7 |
|
LAPS | Lapse due to unpaid annual fee |