ES2106742T3 - Procedimiento de purificacion de proteinas. - Google Patents

Procedimiento de purificacion de proteinas.

Info

Publication number
ES2106742T3
ES2106742T3 ES91107661T ES91107661T ES2106742T3 ES 2106742 T3 ES2106742 T3 ES 2106742T3 ES 91107661 T ES91107661 T ES 91107661T ES 91107661 T ES91107661 T ES 91107661T ES 2106742 T3 ES2106742 T3 ES 2106742T3
Authority
ES
Spain
Prior art keywords
protein
protein purification
purification procedure
antibodies
ion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91107661T
Other languages
English (en)
Inventor
Michael H Coan
Vivian W Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24106028&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2106742(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of ES2106742T3 publication Critical patent/ES2106742T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

METODO PARA PURIFICAR UNA PROTEINA DE UNA SOLUCION DE SUSTANCIAS QUE COMPRENDE EL PASO DE LA SOLUCION A TRAVES DE UN MATERIAL DE INTERCAMBIO DE IONES A UN PH QUE FACILITA LA UNION DE LA PROTEINA DE INTERES, LAVANDO LA PROTEINA UNIDA A UN PH DIFERENTE AL QUE LA PROTEINA UNIDA NO SE ELUTE PERO AL CUAL LA PROTEINA LIBRE NO SE UNE AL MATERIAL DE INTERCAMBIO DE IONES, Y LUEGO ELUTIENDO LA PROTEINA A UN PH QUE FACILITA LA ELUTION. EL METODO ES DE PARTICULAR UTILIDAD PARA PURIFICAR LOS ANTICUERPOS TALES COMO LOS ANTICUERPOS A UN FACTOR DE NECROSIS DE UN TUMOR.
ES91107661T 1990-05-25 1991-05-11 Procedimiento de purificacion de proteinas. Expired - Lifetime ES2106742T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/528,523 US5110913A (en) 1990-05-25 1990-05-25 Antibody purification method

Publications (1)

Publication Number Publication Date
ES2106742T3 true ES2106742T3 (es) 1997-11-16

Family

ID=24106028

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91107661T Expired - Lifetime ES2106742T3 (es) 1990-05-25 1991-05-11 Procedimiento de purificacion de proteinas.

Country Status (9)

Country Link
US (1) US5110913A (es)
EP (1) EP0460426B1 (es)
JP (1) JP3262128B2 (es)
AT (1) ATE158302T1 (es)
CA (1) CA2042945C (es)
DE (1) DE69127657T2 (es)
DK (1) DK0460426T3 (es)
ES (1) ES2106742T3 (es)
GR (1) GR3025504T3 (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2108460T3 (es) * 1993-06-03 1997-12-16 Therapeutic Antibodies Inc Fragmentos de anticuerpos en terapeutica.
US5759864A (en) * 1995-06-23 1998-06-02 Cedars Sinai Medical Center Methods for reducing background binding in antibody preparations
TW505655B (en) * 1997-10-14 2002-10-11 Tanox Inc Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography
EP1308455B9 (en) * 1998-05-06 2006-06-14 Genentech, Inc. A composition comprising anti-HER2 antibodies
ES2261589T3 (es) * 1998-05-06 2006-11-16 Genentech, Inc. Composicion de anticuerpos anti-her2.
JP2002517406A (ja) 1998-06-01 2002-06-18 ジェネンテック・インコーポレーテッド イオン交換クロマトグラフィーの使用による、凝集体からのタンパク質モノマーの分離
MXPA05005921A (es) * 2002-12-02 2005-10-19 Abgenix Inc Anticuerpos dirigidos a factor de necrosis tumoral y sus usos.
US7662930B2 (en) * 2005-12-06 2010-02-16 Amgen Inc. Polishing steps used in multi-step protein purification processes
EP2738179A1 (en) 2006-04-05 2014-06-04 AbbVie Biotechnology Ltd Antibody purification
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
NZ597334A (en) 2006-09-13 2014-02-28 Abbott Lab Cell culture improvements
TWI442965B (zh) * 2006-11-01 2014-07-01 Biogen Idec Inc 使用低ｐＨ及二價陽離子分離生物巨分子的方法
ES2666170T3 (es) 2007-10-30 2018-05-03 Genentech, Inc. Purificación de anticuerpos mediante cromatografía de intercambio catiónico
BRPI0911249A2 (pt) * 2008-04-16 2017-05-23 Biogen Idec Inc método de isolamento de biomacromoléculas usando polialquileno glicol e metais de transição
RU2551237C2 (ru) 2008-10-20 2015-05-20 Эббви Инк Инактивация вируса при очистке антител
ES2535734T3 (es) 2008-10-20 2015-05-14 Abbvie Inc. Aislamiento y purificación de anticuerpos mediante cromatografía de afinidad con la proteína A
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
SG11201504249XA (en) 2012-09-02 2015-07-30 Abbvie Inc Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014143205A1 (en) 2013-03-12 2014-09-18 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014159554A1 (en) * 2013-03-14 2014-10-02 Abbvie Inc. Low acidic species compositions and methods for producing the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105315369B (zh) * 2014-07-25 2020-03-13 山东博安生物技术有限公司 利用阳离子交换层析纯化蛋白质

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6019733B2 (ja) * 1977-06-10 1985-05-17 科研製薬株式会社 蛋白質性活性物質
DE3526096A1 (de) * 1985-07-22 1987-01-22 Basf Ag Verfahren zur reinigung von htnf
AT399095B (de) * 1986-03-27 1995-03-27 Vukovich Thomas Dr Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens
US4771128A (en) * 1986-10-10 1988-09-13 Cetus Corporation Method of purifying toxin conjugates using hydrophobic interaction chromatography
WO1989003840A1 (en) * 1987-10-23 1989-05-05 Schering Corporation Method of purifying protein
JPH01247096A (ja) * 1988-03-30 1989-10-02 Mitsubishi Petrochem Co Ltd モノクローナル抗体の回収方法

Also Published As

Publication number Publication date
ATE158302T1 (de) 1997-10-15
EP0460426A2 (en) 1991-12-11
EP0460426A3 (en) 1992-07-22
CA2042945A1 (en) 1991-11-26
DE69127657D1 (de) 1997-10-23
DE69127657T2 (de) 1998-01-15
CA2042945C (en) 2004-05-18
EP0460426B1 (en) 1997-09-17
JPH06316593A (ja) 1994-11-15
GR3025504T3 (en) 1998-02-27
US5110913A (en) 1992-05-05
DK0460426T3 (da) 1998-05-04
JP3262128B2 (ja) 2002-03-04

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