KR910015699A - 리포코틴의 정제방법 - Google Patents
리포코틴의 정제방법 Download PDFInfo
- Publication number
- KR910015699A KR910015699A KR1019910001997A KR910001997A KR910015699A KR 910015699 A KR910015699 A KR 910015699A KR 1019910001997 A KR1019910001997 A KR 1019910001997A KR 910001997 A KR910001997 A KR 910001997A KR 910015699 A KR910015699 A KR 910015699A
- Authority
- KR
- South Korea
- Prior art keywords
- lipocotin
- solution
- rpp4
- exchanger
- purifying
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4721—Lipocortins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Pain & Pain Management (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (9)
- 리포코틴 용액에 킬레이트제 및 세제를 가하고 이 용액을 교환기와 접촉시킨 다음 액체를 분리해냄을 특징으로 하여, 리포코틴 용액을 음이온 교환기로 처리함으로써 리포코틴을 정제하는 방법.
- 제1항에 있어서, 교환기를 세제-비함유 완충용액으로 세척하고 리포코틴을 용출시키는 방법.
- 제1항에 있어서, 리포코틴이 액체로부터 수득되는 방법.
- 제1항에 있어서, 리포코틴이 유전자 조작으로 제조한 리포코틴인 방법.
- 제1항에 있어서, 리포코틴이 rPP4, rPP4-X 또는 rPP4-델타인 방법.
- 제1항에 있어서, 리포코틴-함유 용액이 이. 클라이, 효모 또는 동물세포 배양액으로부터 유도되는 방법.
- 제1항에 있어서, 리포코틴을 삭카라이드의 고정된 폴리황산 에스테르 또는 담체-결합된 황산화당 위에서 크로마토그래피로 추가 정제하는 방법.
- 제1항에 있어서, 리포코틴을 칼슘 이온 및 계속해서 EDTA의 존재하에 삭카라이드의 고정된 폴리황산에스테르 또는 담체-결합된 황산화당 위에서 크로마토 그래피로 추가 정제하는 방법.
- 제1항에 있어서, 리포코틴을 카올린.RAerisil 또는 인산칼슘상에서 흡착에 의하여 추가 정제하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4003773A DE4003773A1 (de) | 1990-02-08 | 1990-02-08 | Verfahren zur reinigung von lipocortinen |
DEP4003773.8 | 1990-02-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR910015699A true KR910015699A (ko) | 1991-09-30 |
Family
ID=6399691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019910001997A KR910015699A (ko) | 1990-02-08 | 1991-02-06 | 리포코틴의 정제방법 |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0441274A3 (ko) |
JP (1) | JPH0795894A (ko) |
KR (1) | KR910015699A (ko) |
AU (1) | AU7083191A (ko) |
CA (1) | CA2035927A1 (ko) |
DE (1) | DE4003773A1 (ko) |
IE (1) | IE910410A1 (ko) |
PT (1) | PT96693A (ko) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3910240A1 (de) * | 1989-03-30 | 1990-10-04 | Behringwerke Ag | Pp4-delta, seine herstellung und verwendung |
DE3911629A1 (de) * | 1989-04-10 | 1990-10-11 | Behringwerke Ag | Verfahren zur abtrennung von toxinen aus proteinloesungen |
GB2542391A (en) | 2015-09-17 | 2017-03-22 | Annexin Pharmaceuticals Ab | Process of manufacture |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3315000A1 (de) * | 1983-04-26 | 1984-10-31 | Behringwerke Ag, 3550 Marburg | Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung |
DE3643182A1 (de) * | 1986-12-18 | 1988-06-30 | Behringwerke Ag | Arzneimittel enthaltend das gewebeprotein pp4, verfahren zur herstellung von pp4 und zu seiner pasteurisierung sowie die verwendung von pp4 |
JP2660514B2 (ja) * | 1987-02-20 | 1997-10-08 | 興和株式会社 | 抗血液凝固作用を有するポリペプチド |
EP0286830A3 (de) * | 1987-03-13 | 1990-01-24 | BEHRINGWERKE Aktiengesellschaft | Verfahren zur Extraktion von Protein PP4 aus Geweben und die Verwendung von Citronensäure für diesen Zweck |
DE3724726A1 (de) * | 1987-07-25 | 1989-02-02 | Behringwerke Ag | Verfahren zur reinigung des plazentaren gewebeproteins pp4 |
DE3911629A1 (de) * | 1989-04-10 | 1990-10-11 | Behringwerke Ag | Verfahren zur abtrennung von toxinen aus proteinloesungen |
-
1990
- 1990-02-08 DE DE4003773A patent/DE4003773A1/de not_active Withdrawn
-
1991
- 1991-02-02 EP EP19910101409 patent/EP0441274A3/de not_active Withdrawn
- 1991-02-06 KR KR1019910001997A patent/KR910015699A/ko not_active Application Discontinuation
- 1991-02-07 JP JP3036501A patent/JPH0795894A/ja active Pending
- 1991-02-07 AU AU70831/91A patent/AU7083191A/en not_active Abandoned
- 1991-02-07 CA CA002035927A patent/CA2035927A1/en not_active Abandoned
- 1991-02-07 PT PT96693A patent/PT96693A/pt unknown
- 1991-02-07 IE IE041091A patent/IE910410A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
IE910410A1 (en) | 1991-08-14 |
CA2035927A1 (en) | 1991-08-09 |
DE4003773A1 (de) | 1991-08-14 |
PT96693A (pt) | 1991-10-31 |
EP0441274A2 (de) | 1991-08-14 |
EP0441274A3 (en) | 1991-12-18 |
AU7083191A (en) | 1991-08-15 |
JPH0795894A (ja) | 1995-04-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |