KR910015699A - 리포코틴의 정제방법 - Google Patents

리포코틴의 정제방법 Download PDF

Info

Publication number
KR910015699A
KR910015699A KR1019910001997A KR910001997A KR910015699A KR 910015699 A KR910015699 A KR 910015699A KR 1019910001997 A KR1019910001997 A KR 1019910001997A KR 910001997 A KR910001997 A KR 910001997A KR 910015699 A KR910015699 A KR 910015699A
Authority
KR
South Korea
Prior art keywords
lipocotin
solution
rpp4
exchanger
purifying
Prior art date
Application number
KR1019910001997A
Other languages
English (en)
Inventor
뢰미쉬 위르겐
아만 에곤
그로테 마티아스
Original Assignee
스타인, 부크
베링베르케 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 스타인, 부크, 베링베르케 아크티엔게젤샤프트 filed Critical 스타인, 부크
Publication of KR910015699A publication Critical patent/KR910015699A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Rheumatology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Pain & Pain Management (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

리포코틴의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (9)

  1. 리포코틴 용액에 킬레이트제 및 세제를 가하고 이 용액을 교환기와 접촉시킨 다음 액체를 분리해냄을 특징으로 하여, 리포코틴 용액을 음이온 교환기로 처리함으로써 리포코틴을 정제하는 방법.
  2. 제1항에 있어서, 교환기를 세제-비함유 완충용액으로 세척하고 리포코틴을 용출시키는 방법.
  3. 제1항에 있어서, 리포코틴이 액체로부터 수득되는 방법.
  4. 제1항에 있어서, 리포코틴이 유전자 조작으로 제조한 리포코틴인 방법.
  5. 제1항에 있어서, 리포코틴이 rPP4, rPP4-X 또는 rPP4-델타인 방법.
  6. 제1항에 있어서, 리포코틴-함유 용액이 이. 클라이, 효모 또는 동물세포 배양액으로부터 유도되는 방법.
  7. 제1항에 있어서, 리포코틴을 삭카라이드의 고정된 폴리황산 에스테르 또는 담체-결합된 황산화당 위에서 크로마토그래피로 추가 정제하는 방법.
  8. 제1항에 있어서, 리포코틴을 칼슘 이온 및 계속해서 EDTA의 존재하에 삭카라이드의 고정된 폴리황산에스테르 또는 담체-결합된 황산화당 위에서 크로마토 그래피로 추가 정제하는 방법.
  9. 제1항에 있어서, 리포코틴을 카올린.RAerisil 또는 인산칼슘상에서 흡착에 의하여 추가 정제하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910001997A 1990-02-08 1991-02-06 리포코틴의 정제방법 KR910015699A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4003773A DE4003773A1 (de) 1990-02-08 1990-02-08 Verfahren zur reinigung von lipocortinen
DEP4003773.8 1990-02-08

Publications (1)

Publication Number Publication Date
KR910015699A true KR910015699A (ko) 1991-09-30

Family

ID=6399691

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019910001997A KR910015699A (ko) 1990-02-08 1991-02-06 리포코틴의 정제방법

Country Status (8)

Country Link
EP (1) EP0441274A3 (ko)
JP (1) JPH0795894A (ko)
KR (1) KR910015699A (ko)
AU (1) AU7083191A (ko)
CA (1) CA2035927A1 (ko)
DE (1) DE4003773A1 (ko)
IE (1) IE910410A1 (ko)
PT (1) PT96693A (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3910240A1 (de) * 1989-03-30 1990-10-04 Behringwerke Ag Pp4-delta, seine herstellung und verwendung
DE3911629A1 (de) * 1989-04-10 1990-10-11 Behringwerke Ag Verfahren zur abtrennung von toxinen aus proteinloesungen
GB2542391A (en) 2015-09-17 2017-03-22 Annexin Pharmaceuticals Ab Process of manufacture

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3315000A1 (de) * 1983-04-26 1984-10-31 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
DE3643182A1 (de) * 1986-12-18 1988-06-30 Behringwerke Ag Arzneimittel enthaltend das gewebeprotein pp4, verfahren zur herstellung von pp4 und zu seiner pasteurisierung sowie die verwendung von pp4
JP2660514B2 (ja) * 1987-02-20 1997-10-08 興和株式会社 抗血液凝固作用を有するポリペプチド
EP0286830A3 (de) * 1987-03-13 1990-01-24 BEHRINGWERKE Aktiengesellschaft Verfahren zur Extraktion von Protein PP4 aus Geweben und die Verwendung von Citronensäure für diesen Zweck
DE3724726A1 (de) * 1987-07-25 1989-02-02 Behringwerke Ag Verfahren zur reinigung des plazentaren gewebeproteins pp4
DE3911629A1 (de) * 1989-04-10 1990-10-11 Behringwerke Ag Verfahren zur abtrennung von toxinen aus proteinloesungen

Also Published As

Publication number Publication date
IE910410A1 (en) 1991-08-14
CA2035927A1 (en) 1991-08-09
DE4003773A1 (de) 1991-08-14
PT96693A (pt) 1991-10-31
EP0441274A2 (de) 1991-08-14
EP0441274A3 (en) 1991-12-18
AU7083191A (en) 1991-08-15
JPH0795894A (ja) 1995-04-11

Similar Documents

Publication Publication Date Title
ES8100502A1 (es) Un procedimiento para purificar proteinas con pesos moleculares superiores a 12.000,en particular interferon
SE9500778D0 (sv) Process for producing a protein
DK0775150T3 (da) Fremgangsmåde til reduktion eller fjernelse af endotoksiner
KR870001834A (ko) 일본뇌염 바이러스의 정제방법
CA2060314A1 (en) Low electroendosmosis agarose
KR870001836A (ko) 광견병 바이러스의 정제방법
FR2570081B1 (fr) Polysaccharides membranaires utiles notamment comme medicament et procede pour leur preparation
KR910015699A (ko) 리포코틴의 정제방법
KR830006324A (ko) 루틴 데카 또는 노나(수소-황산염)나트륨염의 제조방법
NL7017514A (en) Purification of proteins
RU2006123543A (ru) Способ очистки интерферона бета
Dorai et al. Fractionation of sialoglycoproteins on an immobilized sialic acid-binding lectin
ES553804A0 (es) Procedimiento para purificar un activador de plasminogeno
ES456196A1 (es) Un metodo para la preparacion de heparina de elevada activi-dad especifica.
KR940005284A (ko) 면역친화성 크로마토그래피에 의한 플라스미노겐 활성화제 억제제 2의 정제방법
KR900004933A (ko) 조직 플라스미노겐 액티베이터의 정제방법
KR830009779A (ko) 항종양 생리학적 활성물질의 정제방법
KR930000681A (ko) 한국형 c형 감염 바이러스의 특이항원인 khcv mbp-코아(core) 17 단백질의 정제 방법
Shpanchenko et al. Isolation, purification and properties of cellobiohydrolase from Trichoderma longibrachiatum.
KR910009727A (ko) 리포코르틴의 정제방법
ATE55253T1 (de) Glycopeptide und glycoproteine, jede dieselben enthaltende zusammensetzung, verfahren zu ihrer herstellung und ihre antikoagulierenden verwendungen.
KR860000868A (ko) Lpf-ha의 정제방법
RU1839190C (ru) Способ очистки микробной эстеразы
GB1425918A (en) Process for enzyme purification
JPS57140726A (en) Purification of physiologically active substance having carcinostatic action

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid