GB1425918A - Process for enzyme purification - Google Patents

Process for enzyme purification

Info

Publication number
GB1425918A
GB1425918A GB4153872A GB4153872A GB1425918A GB 1425918 A GB1425918 A GB 1425918A GB 4153872 A GB4153872 A GB 4153872A GB 4153872 A GB4153872 A GB 4153872A GB 1425918 A GB1425918 A GB 1425918A
Authority
GB
United Kingdom
Prior art keywords
lyase
groups
cross
dextran gel
active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4153872A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biorex Laboratories Ltd
Original Assignee
Biorex Laboratories Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biorex Laboratories Ltd filed Critical Biorex Laboratories Ltd
Priority to GB4153872A priority Critical patent/GB1425918A/en
Publication of GB1425918A publication Critical patent/GB1425918A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1425918 Hyaluronidases BIOREX LABORATORIES Ltd 22 Oct 1973 [7 Sept 1972] 41538/72 Heading C3H Glucuronoglycosaminoglycan hyaluronate lyase is obtained in very pure and highly active form by first contacting the crude lyase with a cross-linked polyacrylic divinyl-benzene resin containing carboxyl groups as active groups, eluting the adsorbed lyase from the resin, dialysing the eluate fractions rich in the lyase against a saturated solution of ammonium sulphate and further purifying the resulting enzyme concentrate by adsorption of the enzyme on, and elution from, a cross-linked dextran gel. A dextran gel containing active carboxymethyl groups is preferably employed, particularly in the final stage of purification.
GB4153872A 1972-09-07 1972-09-07 Process for enzyme purification Expired GB1425918A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB4153872A GB1425918A (en) 1972-09-07 1972-09-07 Process for enzyme purification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4153872A GB1425918A (en) 1972-09-07 1972-09-07 Process for enzyme purification

Publications (1)

Publication Number Publication Date
GB1425918A true GB1425918A (en) 1976-02-25

Family

ID=10420160

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4153872A Expired GB1425918A (en) 1972-09-07 1972-09-07 Process for enzyme purification

Country Status (1)

Country Link
GB (1) GB1425918A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4152212A (en) * 1976-02-25 1979-05-01 Biorex Laboratories Limited Process for the purification of glucuronoglycosaminoglycan hyaluronate lyase
FR2495634A1 (en) * 1980-12-08 1982-06-11 Biorex Laboratories Ltd ENZYME NEW AND USEFUL IN THERAPEUTICS

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4152212A (en) * 1976-02-25 1979-05-01 Biorex Laboratories Limited Process for the purification of glucuronoglycosaminoglycan hyaluronate lyase
FR2495634A1 (en) * 1980-12-08 1982-06-11 Biorex Laboratories Ltd ENZYME NEW AND USEFUL IN THERAPEUTICS

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee