FR2190413A2 - Stable,pyrogen-free urokinase prepns - from human urine - Google Patents
Stable,pyrogen-free urokinase prepns - from human urineInfo
- Publication number
- FR2190413A2 FR2190413A2 FR7223868A FR7223868A FR2190413A2 FR 2190413 A2 FR2190413 A2 FR 2190413A2 FR 7223868 A FR7223868 A FR 7223868A FR 7223868 A FR7223868 A FR 7223868A FR 2190413 A2 FR2190413 A2 FR 2190413A2
- Authority
- FR
- France
- Prior art keywords
- hos
- urokinase
- conductivity
- prepns
- pyrogen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Crude urokinase extracts from human and urine, are purified by (a) two stages of exclusion chromatography on DEAE cellulose, the first at pH 4-6 and conductivity 15000-25000 mu m hos/cm, and the second at pH 6-7 and conductivity 15000-18000 mu m hos/cm initially, lowered in stages to 9000 mu m hos/cm before elution. The quantity of adsorbent must be proportional to the urokinase, at 70-60g. dry resin/107 CTA units, (b) further chromatographic purificn. (opt.) (c) fractional pptn. at pH 3-4.5, conductivity 15000-25000 mu m hos/cm and concn. 1.5-3 mg/ml, using a 25-35% satn. of (NH4)2SO4, and to remove pyrogens, (d) complex formation with heparin to stabilize the product.
Priority Applications (21)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE789189D BE789189A (en) | 1971-09-24 | IMPROVEMENTS IN THE PROCESSES FOR PREPARATION, DEPYROGENATION AND STABILIZATION OF UROKINASE AND COMPOSITIONS OBTAINED | |
FR7223868A FR2190413A2 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
SE7212220A SE405806B (en) | 1971-09-24 | 1972-09-21 | PROCEDURE FOR PREPARING UROKINAS HEPARIN COMPLEX |
CH1384072A CH560050A5 (en) | 1971-09-24 | 1972-09-22 | |
DK470672A DK133130C (en) | 1971-09-24 | 1972-09-22 | PROCEDURE FOR PREPARING UROKINASE HEPARINATE |
JP9561172A JPS5718465B2 (en) | 1971-09-24 | 1972-09-22 | |
GB1550275A GB1411422A (en) | 1971-09-24 | 1972-09-22 | Methods for purifying urokinase preparations |
CH1715974A CH604726A5 (en) | 1971-09-24 | 1972-09-22 | |
NL7212891A NL7212891A (en) | 1971-09-24 | 1972-09-22 | |
GB4400372A GB1411421A (en) | 1971-09-24 | 1972-09-22 | Urokinaseheparin complex |
CA152,328A CA986013A (en) | 1971-09-24 | 1972-09-22 | Methods for preparing depyrogenising and stabilising urokinase and compositions obtained thereby |
ES406980A ES406980A1 (en) | 1971-09-24 | 1972-09-23 | Urokinaseheparin complex |
US292139A US3926727A (en) | 1971-09-24 | 1972-09-25 | Urokinase preparations |
DE2246969A DE2246969C2 (en) | 1971-09-24 | 1972-09-25 | Urokinase heparinate and medicinal products containing it |
IN2122/CAL/1973A IN141112B (en) | 1971-09-24 | 1973-09-17 | |
ES440244A ES440244A1 (en) | 1971-09-24 | 1975-08-14 | Urokinaseheparin complex |
US05/629,076 US4029767A (en) | 1971-09-24 | 1975-11-05 | Pharmaceutical compositions of stable urokinase-heparin complexes and methods for use thereof |
US05/628,968 US4106992A (en) | 1971-09-24 | 1975-11-05 | Purification of urokinase |
SE7514792A SE7514792L (en) | 1971-09-24 | 1975-12-30 | PROCEDURE FOR THE PRODUCTION OF UROKINAS |
ES455975A ES455975A1 (en) | 1971-09-24 | 1977-02-16 | Urokinaseheparin complex |
NLAANVRAGE8300232,A NL183495C (en) | 1971-09-24 | 1983-01-21 | METHOD FOR PREPARING AN UROKINASE PERFUSION SOLUTION AND METHOD FOR PREPARING AN UROKINASE HEPARINATE SUITABLE FOR SUCH USE |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7223868A FR2190413A2 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2190413A2 true FR2190413A2 (en) | 1974-02-01 |
FR2190413B2 FR2190413B2 (en) | 1975-06-20 |
Family
ID=9101199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7223868A Granted FR2190413A2 (en) | 1971-09-24 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR2190413A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2291983A1 (en) * | 1974-11-20 | 1976-06-18 | Abbott Lab | PURIFICATION OF UROKINASE |
FR2395275A1 (en) * | 1977-03-10 | 1979-01-19 | Sumitomo Chemical Co | UROKINASE PURIFICATION PROCESS |
-
1972
- 1972-06-30 FR FR7223868A patent/FR2190413A2/en active Granted
Non-Patent Citations (1)
Title |
---|
NEANT * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2291983A1 (en) * | 1974-11-20 | 1976-06-18 | Abbott Lab | PURIFICATION OF UROKINASE |
FR2395275A1 (en) * | 1977-03-10 | 1979-01-19 | Sumitomo Chemical Co | UROKINASE PURIFICATION PROCESS |
Also Published As
Publication number | Publication date |
---|---|
FR2190413B2 (en) | 1975-06-20 |
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