FR2153200A1 - Stable,pyrogen-free urokinase prepns - from human urine - Google Patents

Stable,pyrogen-free urokinase prepns - from human urine

Info

Publication number
FR2153200A1
FR2153200A1 FR7134435A FR7134435A FR2153200A1 FR 2153200 A1 FR2153200 A1 FR 2153200A1 FR 7134435 A FR7134435 A FR 7134435A FR 7134435 A FR7134435 A FR 7134435A FR 2153200 A1 FR2153200 A1 FR 2153200A1
Authority
FR
France
Prior art keywords
hos
urokinase
conductivity
prepns
pyrogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7134435A
Other languages
French (fr)
Other versions
FR2153200B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Choay SA
Original Assignee
Choay SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BE789189D priority Critical patent/BE789189A/en
Application filed by Choay SA filed Critical Choay SA
Priority to FR7134435A priority patent/FR2153200A1/en
Priority to SE7212220A priority patent/SE405806B/en
Priority to ZA726490A priority patent/ZA726490B/en
Priority to DK470672A priority patent/DK133130C/en
Priority to JP9561172A priority patent/JPS5718465B2/ja
Priority to CH1715974A priority patent/CH604726A5/xx
Priority to GB1550275A priority patent/GB1411422A/en
Priority to GB4400372A priority patent/GB1411421A/en
Priority to CA152,328A priority patent/CA986013A/en
Priority to NL7212891A priority patent/NL7212891A/xx
Priority to CH1384072A priority patent/CH560050A5/xx
Priority to ES406980A priority patent/ES406980A1/en
Priority to DE2246969A priority patent/DE2246969C2/en
Priority to AU47051/72A priority patent/AU488913B2/en
Publication of FR2153200A1 publication Critical patent/FR2153200A1/en
Priority to IN2122/CAL/1973A priority patent/IN141112B/en
Application granted granted Critical
Publication of FR2153200B1 publication Critical patent/FR2153200B1/fr
Priority to ES440244A priority patent/ES440244A1/en
Priority to US05/629,076 priority patent/US4029767A/en
Priority to SE7514792A priority patent/SE7514792L/en
Priority to ES455975A priority patent/ES455975A1/en
Priority to NLAANVRAGE8300232,A priority patent/NL183495C/en
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Crude urokinase extracts from human and urine, are purified by (a) two stages of exclusion chromatography on DEAE cellulose, the first at pH 4-6 and conductivity 15000-25000 mu m hos/cm, and the second at pH 6-7 and conductivity 15000-18000 mu m hos/cm initially, lowered in stages to 9000 mu m hos/cm before elution. The quantity of adsorbent must be proportional to the urokinase, at 70-60g. dry resin/107 CTA units, (b) further chromatographic purificn. (opt.) (c) fractional pptn. at pH 3-4.5, conductivity 15000-25000 mu m hos/cm and concn. 1.5-3 mg/ml, using a 25-35% satn. of (NH4)2SO4, and to remove pyrogens, (d) complex formation with heparin to stabilize the product.
FR7134435A 1971-09-24 1971-09-24 Stable,pyrogen-free urokinase prepns - from human urine Granted FR2153200A1 (en)

Priority Applications (21)

Application Number Priority Date Filing Date Title
BE789189D BE789189A (en) 1971-09-24 IMPROVEMENTS IN THE PROCESSES FOR PREPARATION, DEPYROGENATION AND STABILIZATION OF UROKINASE AND COMPOSITIONS OBTAINED
FR7134435A FR2153200A1 (en) 1971-09-24 1971-09-24 Stable,pyrogen-free urokinase prepns - from human urine
SE7212220A SE405806B (en) 1971-09-24 1972-09-21 PROCEDURE FOR PREPARING UROKINAS HEPARIN COMPLEX
NL7212891A NL7212891A (en) 1971-09-24 1972-09-22
DK470672A DK133130C (en) 1971-09-24 1972-09-22 PROCEDURE FOR PREPARING UROKINASE HEPARINATE
JP9561172A JPS5718465B2 (en) 1971-09-24 1972-09-22
CH1715974A CH604726A5 (en) 1971-09-24 1972-09-22
GB1550275A GB1411422A (en) 1971-09-24 1972-09-22 Methods for purifying urokinase preparations
GB4400372A GB1411421A (en) 1971-09-24 1972-09-22 Urokinaseheparin complex
CA152,328A CA986013A (en) 1971-09-24 1972-09-22 Methods for preparing depyrogenising and stabilising urokinase and compositions obtained thereby
ZA726490A ZA726490B (en) 1971-09-24 1972-09-22 Improvements in methods for preparing depyrogenising and stabilising urokinase and compositions obtained thereby
CH1384072A CH560050A5 (en) 1971-09-24 1972-09-22
ES406980A ES406980A1 (en) 1971-09-24 1972-09-23 Urokinaseheparin complex
DE2246969A DE2246969C2 (en) 1971-09-24 1972-09-25 Urokinase heparinate and medicinal products containing it
AU47051/72A AU488913B2 (en) 1972-09-25 Improvements in methods for preparing depyrogenising and stabilising urokinase and compositions obtained thereby
IN2122/CAL/1973A IN141112B (en) 1971-09-24 1973-09-17
ES440244A ES440244A1 (en) 1971-09-24 1975-08-14 Urokinaseheparin complex
US05/629,076 US4029767A (en) 1971-09-24 1975-11-05 Pharmaceutical compositions of stable urokinase-heparin complexes and methods for use thereof
SE7514792A SE7514792L (en) 1971-09-24 1975-12-30 PROCEDURE FOR THE PRODUCTION OF UROKINAS
ES455975A ES455975A1 (en) 1971-09-24 1977-02-16 Urokinaseheparin complex
NLAANVRAGE8300232,A NL183495C (en) 1971-09-24 1983-01-21 METHOD FOR PREPARING AN UROKINASE PERFUSION SOLUTION AND METHOD FOR PREPARING AN UROKINASE HEPARINATE SUITABLE FOR SUCH USE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7134435A FR2153200A1 (en) 1971-09-24 1971-09-24 Stable,pyrogen-free urokinase prepns - from human urine

Publications (2)

Publication Number Publication Date
FR2153200A1 true FR2153200A1 (en) 1973-05-04
FR2153200B1 FR2153200B1 (en) 1974-10-18

Family

ID=9083444

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7134435A Granted FR2153200A1 (en) 1971-09-24 1971-09-24 Stable,pyrogen-free urokinase prepns - from human urine

Country Status (2)

Country Link
FR (1) FR2153200A1 (en)
ZA (1) ZA726490B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2310133A2 (en) * 1975-05-05 1976-12-03 Fabre Sa Pierre NEW MEDICINAL PRODUCT CONTAINING AN IMPROVED PLASMINOGEN ACTIVATOR

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2310133A2 (en) * 1975-05-05 1976-12-03 Fabre Sa Pierre NEW MEDICINAL PRODUCT CONTAINING AN IMPROVED PLASMINOGEN ACTIVATOR

Also Published As

Publication number Publication date
ZA726490B (en) 1973-06-27
AU4705172A (en) 1974-04-04
FR2153200B1 (en) 1974-10-18

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Legal Events

Date Code Title Description
JD Different final judgements
ST Notification of lapse