GB1411421A - Urokinaseheparin complex - Google Patents
Urokinaseheparin complexInfo
- Publication number
- GB1411421A GB1411421A GB4400372A GB4400372A GB1411421A GB 1411421 A GB1411421 A GB 1411421A GB 4400372 A GB4400372 A GB 4400372A GB 4400372 A GB4400372 A GB 4400372A GB 1411421 A GB1411421 A GB 1411421A
- Authority
- GB
- United Kingdom
- Prior art keywords
- urokinase
- complex
- heparin
- conductivity
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
1411421 Urokinase-heparin complex CHOAY SA 22 Sept 1972 [24 Sept 1971 30 June 1972 (2)] 44003/72 Heading C3H [Also in Division A5] A complex of urokinase and heparin is prepared by admixing a solution of urokinase and a solution of heparin, adjusting the pH of the mixed solutions, e.g. with phosphoric acid, to from 1 to 5, preferably about 4.2, for formation and precipitation of the complex and, if desired, freeze-drying the complex. The complex may have typical relative activities of 30,000-100,000 CTA units of -urokinase per 100 IU of heparin. A solution for perfusion comprises the complex dissolved in a pharmaceutically acceptable, isotonic solution, e.g. 5% glucose serum, which is compatible with urokinase, does not form a precipitate with heparin and is free of mineral salts. The solutions may have urokinase concentrations of the order 300,000-900,000 CTA units/250 ml. Methods for obtaining a pyrogenic urokinase as starting material for the complex formation are described in detail. They may involve extracting crude urokinase from human urine by absorption on diatomaceous earth and elution of urokinase therefrom, elimination of viral contamination as by heating to 60C. under slightly acid conditions and cooling and separating off the solid residue, and two steps of fractionation using diethylaminoethyl cellulose employing in the first step suspensions of pH 4-6 and conductivity 15,000-25,000 micromhos/cm. with 15-40 g. of resin per 14 million CTA units of urokinase, and in the second step suspensions of pH 6-7 and conductivity 15,000-18,000 micromhos/cm. with 30-60 g. of resin per 10 million CTA units of the enzyme. The resulting urokinase-containing medium is diluted with quantities of water sufficient to lower the conductivity by about 1000 microinhos/cm. at each successive dilution until a suspension having a conductivity of about 9,000 micromhos/cm. is obtained.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7134435A FR2153200A1 (en) | 1971-09-24 | 1971-09-24 | Stable,pyrogen-free urokinase prepns - from human urine |
| FR7223868A FR2190413A2 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
| FR7223867A FR2190412A1 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1411421A true GB1411421A (en) | 1975-10-22 |
Family
ID=27249660
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4400372A Expired GB1411421A (en) | 1971-09-24 | 1972-09-22 | Urokinaseheparin complex |
| GB1550275A Expired GB1411422A (en) | 1971-09-24 | 1972-09-22 | Methods for purifying urokinase preparations |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1550275A Expired GB1411422A (en) | 1971-09-24 | 1972-09-22 | Methods for purifying urokinase preparations |
Country Status (11)
| Country | Link |
|---|---|
| JP (1) | JPS5718465B2 (en) |
| BE (1) | BE789189A (en) |
| CA (1) | CA986013A (en) |
| CH (2) | CH604726A5 (en) |
| DE (1) | DE2246969C2 (en) |
| DK (1) | DK133130C (en) |
| ES (3) | ES406980A1 (en) |
| GB (2) | GB1411421A (en) |
| IN (1) | IN141112B (en) |
| NL (1) | NL7212891A (en) |
| SE (2) | SE405806B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2428248B2 (en) * | 1973-07-24 | 1978-01-12 | Serna Ag, Glarus (Schweiz) | PROCESS FOR PRODUCING PYROGEN-FREE UROKINASE |
| US3957582A (en) * | 1974-11-20 | 1976-05-18 | Abbott Laboratories | Purification of urokinase |
| JPS59143694U (en) * | 1983-03-14 | 1984-09-26 | 東洋電機製造株式会社 | Automatic drawing cutting machine press ruling device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3256158A (en) * | 1963-03-22 | 1966-06-14 | Abbott Lab | Purification of urokinase |
| US3355361A (en) * | 1964-10-15 | 1967-11-28 | Sterling Drug Inc | Recovery and purification of urokinase |
| US3477912A (en) * | 1967-07-07 | 1969-11-11 | Century Lab Inc | Method of production of urokinase |
| US3477913A (en) * | 1967-10-31 | 1969-11-11 | Century Lab Inc | Method of production of urokinase |
-
0
- BE BE789189D patent/BE789189A/en not_active IP Right Cessation
-
1972
- 1972-09-21 SE SE7212220A patent/SE405806B/en unknown
- 1972-09-22 CH CH1715974A patent/CH604726A5/xx not_active IP Right Cessation
- 1972-09-22 GB GB4400372A patent/GB1411421A/en not_active Expired
- 1972-09-22 GB GB1550275A patent/GB1411422A/en not_active Expired
- 1972-09-22 DK DK470672A patent/DK133130C/en not_active IP Right Cessation
- 1972-09-22 NL NL7212891A patent/NL7212891A/xx active Search and Examination
- 1972-09-22 JP JP9561172A patent/JPS5718465B2/ja not_active Expired
- 1972-09-22 CH CH1384072A patent/CH560050A5/xx not_active IP Right Cessation
- 1972-09-22 CA CA152,328A patent/CA986013A/en not_active Expired
- 1972-09-23 ES ES406980A patent/ES406980A1/en not_active Expired
- 1972-09-25 DE DE2246969A patent/DE2246969C2/en not_active Expired
-
1973
- 1973-09-17 IN IN2122/CAL/1973A patent/IN141112B/en unknown
-
1975
- 1975-08-14 ES ES440244A patent/ES440244A1/en not_active Expired
- 1975-12-30 SE SE7514792A patent/SE7514792L/en unknown
-
1977
- 1977-02-16 ES ES455975A patent/ES455975A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CA986013A (en) | 1976-03-23 |
| ES440244A1 (en) | 1977-08-01 |
| SE405806B (en) | 1979-01-08 |
| ES455975A1 (en) | 1978-03-01 |
| JPS5718465B2 (en) | 1982-04-16 |
| DK133130C (en) | 1976-09-27 |
| BE789189A (en) | 1973-03-22 |
| JPS4856888A (en) | 1973-08-09 |
| SE7514792L (en) | 1975-12-30 |
| ES406980A1 (en) | 1976-06-16 |
| NL7212891A (en) | 1973-03-27 |
| DE2246969A1 (en) | 1973-04-05 |
| DK133130B (en) | 1976-03-29 |
| IN141112B (en) | 1977-01-22 |
| CH560050A5 (en) | 1975-03-27 |
| CH604726A5 (en) | 1978-09-15 |
| GB1411422A (en) | 1975-10-22 |
| DE2246969C2 (en) | 1986-12-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |