IE910410A1 - A process for purifying lipocortins - Google Patents
A process for purifying lipocortinsInfo
- Publication number
- IE910410A1 IE910410A1 IE041091A IE41091A IE910410A1 IE 910410 A1 IE910410 A1 IE 910410A1 IE 041091 A IE041091 A IE 041091A IE 41091 A IE41091 A IE 41091A IE 910410 A1 IE910410 A1 IE 910410A1
- Authority
- IE
- Ireland
- Prior art keywords
- lipocortin
- rpp4
- solution
- proteins
- exchanger
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4721—Lipocortins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Pain & Pain Management (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A process for the purification of lipocortins, especially of the proteins, prepared by genetic manipulation, rPP4, rPP4-X and rPP4-delta, by treatment of a solution of a lipocortin with an anion exchanger in the presence of a chelating agent and of a detergent is described.
Description
The invention relates to a process for purifying lipocortins, especially the proteins rPP4, rPP4-X and rPP4delta prepared by gene manipulation. These proteins have anticoagulant and antiinflammatory effects.
The natural protein PP4 is described in EP-A 0 123 307.
The preparation of PP4, of a protein PP4-X which also has anticoagulant activity, and of a deletion variant with increased activity (PP4-delta) by gene manipulation is described in DE-A 39 10 240.
These proteins can be regarded as belonging to a group of proteins which are called lipocortins (glucocorticoidinduced phospholipase-inhibiting proteins) or calpactins (calcium-dependent phospholipid- and actin-binding proteins).
The object of the invention is to provide a process for obtaining and purifying lipocortins, especially the proteins PP4, PP4-X and PP4-delta prepared by gene manipulation (rPP4, rPP4-X and rPP4-delta).
Although processes for purifying PP4 are known, they are unsatisfactory for obtaining lipocortins prepared by gene manipulation because the composition of the initial solution differs.
The invention relates to a process for purifying a lipocortin by treating a solution thereof with an anion exchanger, which comprises adding to this solution a chelating agent and a detergent and contacting this solution with the exchanger and separating off the liquid.
It is then possible to wash the exchanger with a deter5 gent-free buffer solution and elute the lipocortin.
However, the lipocortin is obtained from the liquid where appropriate.
The described process is particularly suitable for lipocortins prepared by gene manipulation. A lipocortin of this type is, in particular, rPP4, rPP4-X or rPP4delta. If addition of a chelating agent to a lipocortin solution results in a precipitate, the latter is preferably separated off before adding a detergent.
A lipocortin-containing solution is, in particular, an
E.coli lysate as are produced in gene manipulation processes using E.coli. The chelating reagent is used in a concentration of 0.001-0.05 mol/1, preferably 0.0020.02 mol/1. The pH is adjusted to 6.5-9.5, preferably pH
6.8-7.5. Detergents which can be used are ionic or nonionic or zwitterionic, preferably derivatives of cholic acid and salts thereof and Triton X-100 in a concentration of 0.1-2.0% (generally).
are basic ion DEAE-RSepharose, (a copolymer of
Ion exchangers which can be used exchangers, preferably Q-, QAE- or 25 -cellulose, -RSephadex or -RFractogel pentaerythritol, methacrylic acid derivatives and polyethylene glycol which carries amino groups and is crosslinked with divinylbenzene) or hydroxyapatite. It is possible to carry out adsorption from a buffer solution 30 of 0.01-0.1 mol/1 tris, HEPES or glycine at a pH of 6.59.5.
Whereas rPP4 and rPP4-delta are bound to the anion exchanger under the described conditions, rPP4-X is not bound at pH 6.5-8.0. However, enrichment of this protein is achieved because a large part of the contaminating proteins is adsorbed onto the exchanger.
It has also been found, surprisingly, that rPP4-X and 5 rPP4-delta also, besides rPP4, bind to immobilized polysulfuric acid esters of saccharides or carrier-bound sulfated sugars, but only in the presence of calcium, at a conductivity of 0-10 mS and a pH of 6.5-9.5; the proteins can be eluted by increasing the ionic strength.
These proteins do not bind to these adsorbents in the absence of calcium or in the presence of chelating reagents .
rPP4, rPP4-X or rPP4-delta can be further purified by adsorption onto kaolin or RAerosil in the presence of calcium. Elution is effected by increasing the ionic strength and/or using chelating reagents.
It is also possible to use binding to calcium phosphate for further purification of these proteins. These proteins can be eluted by increasing the ionic strength or using chelating reagents such as EDTA or salts of citric acid or by a mixture containing these reagents.
The lipocortins can be subjected to affinity chromatography for further purification. For this purpose, the lipocortin-containing solutions can be dialyzed, adsorbed in a buffer containing 0.01-0.05 mol/1, preferably 0.02 mol/1, tris-HCl, pH 8.5, after addition of calcium ions in a batch process onto carrier-bound heparin, the adsorbent can be washed, and the proteins can be eluted using a stepped gradient and subsequently dialyzed. The dialysates are mixed with 0.01-20 mmol/l, preferably mmol/l, EDTA, preferably contacted once more with carrier-bound heparin, and the lipocortins dialyzed.
This affinity chromatography can generally be carried out on carrier-bound polysulfuric acid esters of saccharides
or sulfated sugars, for example carrier-bound heparin, dextran sulfate, heparan sulfate, chondroitin sulfate, keratan sulfate or dermatan sulfate, preferably heparinRSepharose or -RFractogel or dextran sulfate-RSepharose or
-RFractogel, from a buffer solution which contains 0.010.1 mol/1 tris, HEPES or glycine, at a pH of 6.5-9.5, preferably pH 6.8-8.5, which preferably contains 5 mmol/1 calcium, and by elution of adsorbed proteins by increasing the ionic strength as for the ion exchanger chromato10 graphy. In the absence of calcium or presence of chelating reagents, rPP4, rPP4-X and rPP4-delta do not bind to these adsorbents.
Further purification can be carried out by adsorption onto RAerosil or kaolin, especially onto RAerosil 200 (from Serva) or kaolin (from Serva) and from E.coli fermentation media (see above) or from a buffer solution with a pH of 6.5-9.5 which contains 0.01-0.1 mol/1 tris, HEPES or glycine and 1-20 mmol/1 calcium. Adsorbed proteins can be eluted by increasing the ionic strength as for the ion exchanger chromatography and/or using chelating reagents, preferably 1-20 mmol/1 EDTA or 0.010.2 mol/1 of a salt of citric acid or a mixture.
In a preferred procedure, lipocortin-containing solutions from E.coli fermentation mixtures or buffer solutions composed of 0.01-0.1 mol/1 tris/HCl, HEPES or glycine and a pH of 6.5-9.5 are mixed with calcium to a final concentration of 1-10 mmol/1, contacted with kaolin, RAerosil 200 or calcium phosphate while shaking, the liquids are separated off, the adsorbent is washed and adsorbed proteins are eluted using a chelating reagent, preferably
EDTA or salts of citric acid or a mixture. After dialysis of the eluates, further purification of the proteins can be continued by adsorption onto a heparin-resin as described above.
Adsorption and elution of the proteins on calcium phosphate is carried out as described for adsorption onto RAerosil and kaolin, but the presence of calcium is not necessary for binding.
A particularly preferred procedure is the combination (EDTA/^riton) DEAE exchanger, then heparin chromatography with Ca2+ and then heparin chromatography with EDTA as described in Example 1.
All the process steps can be carried out at room temperature. Exceptions are indicated.
The following abbreviations are used for the description:
RAerosil 200: silicon dioxide DEAE: diethylaminoethyl EDTA: ethylenediaminetetraacetic acid
HEPES: N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
Kaolin: hydrated aluminum silicate
PAGE: polyacrylamide gel electrophoresis PP4: placental protein 4
Q: quaternary amino RRivanol: 6,9-diamino-2-ethoxyacridine lactate
SDS: sodium dodecyl sulfate
Tris : tris (hydroxymethyl) aminomethane VAC: vascular anticoagulant
The invention is illustrated by the examples which follow:
Example 1
a) Solubilization of phospholipid-associated proteins
1.0 1 of centrifuged E.coli lysates which contained
1.0 mg/ml rPP4 or rPP4-delta in 0.05 mol/1 tris/HCl, pH 7.5, 5 mmol/1 EDTA were mixed with Triton X-100 to a final concentration of 1.0%, incubated at room temperature while shaking for 10 min and then centrifuged.
b) Ion exchanger chromatography
The supernatant after centrifugation was contacted with 300 ml of DEAE-RSepharose (resin material) equilibrated with 0.02 mol/1 tris/HCl, pH 7.5 (column buffer 1), while stirring for one hour, the liquid was removed by filtration, the adsorbent was washed with column buffer 1, and the resin material was packed into a column. Adsorbed proteins were eluted with column buffer 1 which contained 0.25 mol/1 NaCI.
c) Adsorption onto carrier-bound heparin (+calcium)
After dialysis, the ion exchanger eluate (300 ml) was, if necessary, adjusted to a protein concentration of 0.23.0 mg/ml of rPP4 or rPP4-delta after addition of CaCl2 to a final concentration of 5 mmol/l, contacted with
70 ml of heparin-RFractogel equilibrated with 0.02 mol/1 tris/HCl, pH 8.5, 5 mmol/l CaCl2 (column buffer 2), while stirring for one hour, the supernatant liquid was filtered off, the resin was washed with column buffer 2, and adsorbed proteins were eluted by increasing the ionic strength using a 0.3 molar NaCI buffer solution.
d) Heparin chromatography (+EDTA)
After dialysis, the heparin eluate (100 ml) was mixed with EDTA to a final concentration of 1 mmol/l, contacted with 50 ml of heparin-resin, equilibrated with 0.02 M tris/HCl, pH 8.5, 1 mM EDTA (column buffer 3) in a column, and the flow-through containing rPP4 or rPP4delta was dialyzed against a solution of 0.02 M tris/HCl, pH 6.8-8.5, and then lyophilized. After the lyophilisates had been dissolved in appropriate volumes of distilled water or buffer solutions, the recovered biological activities were 95-100% of those of the materials before freeze-drying.
The purity of the rPP4-heparin flow-throughs (d) was
- Ί >95%. The yield was about 55% based on the starting materials and about 40% for rPP4-delta.
Example 2
a) Solubilization of phospholipid-associated proteins
1.0 1 of centrifuged E.coli lysates in 0.02 M tris/HCl, pH 6.8, which contained 0.8 mg/1 rPP4-X at a total protein concentration of about 20 mg/ml was mixed with Triton X-100 to a final concentration of 1.0%, incubated with shaking for 10 min and then centrifuged.
b) Ion exchanger chromatography
The supernatant after centrifugation was contacted with 300 ml of DEAE-Sepharose, equilibrated with 0.02 M tris/HCl, pH 6.8, with stirring, the liquid was removed by filtration, the adsorbent was washed and the resin material was packed into a column. Adsorbed proteins were eluted with a buffer solution which contained 1.5 M NaCl.
The solution containing rPP4-X which was filtered off after the incubation with the ion exchanger resin was adjusted to a pH of 8.5, mixed with 5 mmol/1 CaCl2, incubated with carrier-bound heparin, the adsorbent was washed with a solution of 0.02 M tris/HCl, pH 8.5, adsorbed proteins were eluted, the eluate was dialyzed and, after addition of 1 mmol/1 EDTA, again incubated with heparin-resin as described in Example 1, c. and d.
It was surprisingly found that rPP4-X did not bind to the anion exchanger under these conditions. Nevertheless, a considerable purifying effect was achieved thereby because part of the E.coli proteins were adsorbed onto the resin. The yield after the heparin chromatographies was about 60% based on the starting material, i.e. about
480 mg of rPP4-X with a purity of >95%.
Example 3
Adsorption onto kaolin, RAerosil 200 or calcium phosphate
Centrifuged E.coli lysate solutions from 0.05 M tris/HCl, pH 7.5, which contained 1.0 mg/ml rPP4, rPP4-X or rPP45 delta, with a total protein content of about 20 mg/ml, were mixed with 5 mM CaCl2 and, in separate mixtures, incubated in each case with 25 mg of kaolin or RAerosil 200 per ml of protein solution while stirring for an hour, and the adsorbent was washed with a solution of
0.02 mol/1 tris/HCl, pH 8.0. Adsorption onto calcium phosphate took place under the same conditions but the presence of calcium was unnecessary for binding. Adsorbed proteins were eluted with a solution of 0.02 M tris/HCl, pH 8.0, which contained 0.02 mol/1 EDTA or a mixture of
0.01 M EDTA and 0.1 M sodium citrate.
After the eluates had been dialyzed against a buffer solution of 0.02 M tris/HCl, pH 8.5, the purification of the proteins was continued by adsorption onto carrierbound heparin as described in Example 1, c. and d. The yields of the >95% pure proteins rPP4 and rPP4-X after the heparin chromatographies were between 50 and 60% based on the starting materials.
The proteins rPP4 and rPP4-X purified as described in the Examples were tested for their physicochemical and biological properties . All their properties were identical to those of the corresponding proteins isolated from placenta.
Claims (11)
1. A process for purifying a lipocortin by treating a solution thereof with an anion exchanger, which comprises adding to this solution a chelating agent 5 and a detergent and contacting this solution with the exchanger and separating off the liquid.
2. The process as claimed in claim 1, wherein the exchanger is washed with a detergent-free buffer solution and the lipocortin is eluted.
3. The process as claimed in claim 1, wherein the lipocortin is obtained from the liquid.
4. The process as claimed in claim 1, wherein the lipocortin is a lipocortin prepared by gene manipulation.
5. The process as claimed in claim 1, wherein the lipo15 cortin is rPP4, rPP4-X or rPP4-delta.
6. The process as claimed in claim 1, wherein the lipocortin-containing solution derives from an E.coli, yeast or animal cell culture.
7. The process as claimed in claim 1, wherein the lipo20 cortin is further purified by chromatography on an immobilized polysulfuric acid ester of a saccharide or on a carrier-bound sulfated sugar.
8. The process as claimed in claim 1, wherein the lipocortin is further purified by chromatography on an 25 immobilized polysulfuric acid ester of a saccharide or on a carrier-bound sulfated sugar in the presence of calcium ions and subsequently in the presence of EDTA.
9. The process as claimed in claim 1, wherein the lipocortin is further purified by adsorption onto kaolin, 30 R Aerosil or calcium phosphate.
10. A process according to claim 1 for purifying a lipocortin, substantially as hereinbefore described and exemplified.
11. A lipocortin whenever purified by a process claimed in a preceding claim.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4003773A DE4003773A1 (en) | 1990-02-08 | 1990-02-08 | METHOD FOR PURIFYING LIPOCORTINES |
Publications (1)
Publication Number | Publication Date |
---|---|
IE910410A1 true IE910410A1 (en) | 1991-08-14 |
Family
ID=6399691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE041091A IE910410A1 (en) | 1990-02-08 | 1991-02-07 | A process for purifying lipocortins |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0441274A3 (en) |
JP (1) | JPH0795894A (en) |
KR (1) | KR910015699A (en) |
AU (1) | AU7083191A (en) |
CA (1) | CA2035927A1 (en) |
DE (1) | DE4003773A1 (en) |
IE (1) | IE910410A1 (en) |
PT (1) | PT96693A (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3910240A1 (en) * | 1989-03-30 | 1990-10-04 | Behringwerke Ag | PP4-DELTA, ITS PRODUCTION AND USE |
DE3911629A1 (en) * | 1989-04-10 | 1990-10-11 | Behringwerke Ag | METHOD FOR SEPARATING TOXINES FROM PROTEIN SOLUTIONS |
GB2542391A (en) | 2015-09-17 | 2017-03-22 | Annexin Pharmaceuticals Ab | Process of manufacture |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3315000A1 (en) * | 1983-04-26 | 1984-10-31 | Behringwerke Ag, 3550 Marburg | TISSUE PROTEIN PP (DOWN ARROW) 4 (DOWN ARROW), METHOD FOR ITS RECOVERY AND USE |
DE3643182A1 (en) * | 1986-12-18 | 1988-06-30 | Behringwerke Ag | MEDICINAL PRODUCTS CONTAINING THE TISSUE PROTEIN PP4, METHOD FOR THE PRODUCTION OF PP4 AND ITS PASTEURIZATION AND THE USE OF PP4 |
JP2660514B2 (en) * | 1987-02-20 | 1997-10-08 | 興和株式会社 | Polypeptide having anticoagulant action |
EP0286830A3 (en) * | 1987-03-13 | 1990-01-24 | BEHRINGWERKE Aktiengesellschaft | Process for the extraction of protein pp4 from tissues, and the use of citric acid therefor |
DE3724726A1 (en) * | 1987-07-25 | 1989-02-02 | Behringwerke Ag | METHOD FOR PURIFYING THE PLACENTARY TISSUE PROTEIN PP4 |
DE3911629A1 (en) * | 1989-04-10 | 1990-10-11 | Behringwerke Ag | METHOD FOR SEPARATING TOXINES FROM PROTEIN SOLUTIONS |
-
1990
- 1990-02-08 DE DE4003773A patent/DE4003773A1/en not_active Withdrawn
-
1991
- 1991-02-02 EP EP19910101409 patent/EP0441274A3/en not_active Withdrawn
- 1991-02-06 KR KR1019910001997A patent/KR910015699A/en not_active Application Discontinuation
- 1991-02-07 IE IE041091A patent/IE910410A1/en unknown
- 1991-02-07 JP JP3036501A patent/JPH0795894A/en active Pending
- 1991-02-07 AU AU70831/91A patent/AU7083191A/en not_active Abandoned
- 1991-02-07 PT PT96693A patent/PT96693A/en unknown
- 1991-02-07 CA CA002035927A patent/CA2035927A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JPH0795894A (en) | 1995-04-11 |
EP0441274A2 (en) | 1991-08-14 |
EP0441274A3 (en) | 1991-12-18 |
KR910015699A (en) | 1991-09-30 |
AU7083191A (en) | 1991-08-15 |
PT96693A (en) | 1991-10-31 |
DE4003773A1 (en) | 1991-08-14 |
CA2035927A1 (en) | 1991-08-09 |
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