JPS57135356A - Analysis of hemoglobin - Google Patents

Analysis of hemoglobin

Info

Publication number
JPS57135356A
JPS57135356A JP56020751A JP2075181A JPS57135356A JP S57135356 A JPS57135356 A JP S57135356A JP 56020751 A JP56020751 A JP 56020751A JP 2075181 A JP2075181 A JP 2075181A JP S57135356 A JPS57135356 A JP S57135356A
Authority
JP
Japan
Prior art keywords
soln
prepd
nacl
silica gel
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56020751A
Other languages
Japanese (ja)
Other versions
JPH0151781B2 (en
Inventor
Tadao Hoshino
Makoto Ueki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Yuka Medical Science Co Ltd
Original Assignee
Mitsubishi Yuka Medical Science Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Yuka Medical Science Co Ltd filed Critical Mitsubishi Yuka Medical Science Co Ltd
Priority to JP56020751A priority Critical patent/JPS57135356A/en
Publication of JPS57135356A publication Critical patent/JPS57135356A/en
Publication of JPH0151781B2 publication Critical patent/JPH0151781B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To determine the Hb saccharified (GHb) to grape sugar out of hemoglobin (Hb) directly by adsorbing a sample contg. the Hb in a silica gel packed column, then eluting the same with a phosphoric acid buffer soln. contg. urea or guanidine and NaCl. CONSTITUTION:A sample contg. Hb is adsorbed to the top end of a column packed with silica gel, more particularly, silica gel having carboxy alkyl groups such as carboxy methyl groups and having >=30Angstrom fine pores. On the other hand, an elutate A prepd. by dissolving NaH2OP4 and urea or guanidine in desalted water and controlling pH to 6.50 and a soln. B prepd. by dissolving NaH2PO4 and NaCl and controlling the pH to 6.50 are prepd. and A soln. A discharge valve and a soln. B discharge valve are programed to flow these solns. to the column in such a way that the soln. A and the soln. B have inverse gradient concns. and that the urine is contained from 1mol/l to 0 and NaCl from 0 to 0.2mol/l, whereby the respective fractions of the Hb are eluted. Thereby, the GHb in the Hb is separated and determined accurately and the results are used for diagnosis and treatment of diabetes.
JP56020751A 1981-02-14 1981-02-14 Analysis of hemoglobin Granted JPS57135356A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56020751A JPS57135356A (en) 1981-02-14 1981-02-14 Analysis of hemoglobin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56020751A JPS57135356A (en) 1981-02-14 1981-02-14 Analysis of hemoglobin

Publications (2)

Publication Number Publication Date
JPS57135356A true JPS57135356A (en) 1982-08-20
JPH0151781B2 JPH0151781B2 (en) 1989-11-06

Family

ID=12035886

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56020751A Granted JPS57135356A (en) 1981-02-14 1981-02-14 Analysis of hemoglobin

Country Status (1)

Country Link
JP (1) JPS57135356A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427562A (en) * 1987-07-24 1989-01-30 Nippon Medical Supply Hemoglobin adsorbent
JPH01148268A (en) * 1987-12-07 1989-06-09 Nippon Medical Supply Corp Hemoglobin adsorber
JPH01297554A (en) * 1988-05-25 1989-11-30 Nippon Zoki Pharmaceut Co Ltd Method of measuring glycohemoglobin
JPH03102259A (en) * 1989-09-18 1991-04-26 Hitachi Ltd Method, apparatus, system and separation column of liquid chromatograph

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5257200A (en) * 1975-08-28 1977-05-11 Rhone Poulenc Ind Separation of protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5257200A (en) * 1975-08-28 1977-05-11 Rhone Poulenc Ind Separation of protein

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427562A (en) * 1987-07-24 1989-01-30 Nippon Medical Supply Hemoglobin adsorbent
JPH01148268A (en) * 1987-12-07 1989-06-09 Nippon Medical Supply Corp Hemoglobin adsorber
JPH01297554A (en) * 1988-05-25 1989-11-30 Nippon Zoki Pharmaceut Co Ltd Method of measuring glycohemoglobin
JPH03102259A (en) * 1989-09-18 1991-04-26 Hitachi Ltd Method, apparatus, system and separation column of liquid chromatograph

Also Published As

Publication number Publication date
JPH0151781B2 (en) 1989-11-06

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