DE69402958T2 - Vorrichtung zur quantitativen abbildung von mehreren fluorophoren - Google Patents
Vorrichtung zur quantitativen abbildung von mehreren fluorophorenInfo
- Publication number
- DE69402958T2 DE69402958T2 DE69402958T DE69402958T DE69402958T2 DE 69402958 T2 DE69402958 T2 DE 69402958T2 DE 69402958 T DE69402958 T DE 69402958T DE 69402958 T DE69402958 T DE 69402958T DE 69402958 T2 DE69402958 T2 DE 69402958T2
- Authority
- DE
- Germany
- Prior art keywords
- fluorophores
- fluorophore
- amplifier
- channel
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6423—Spectral mapping, video display
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/011,881 US5294799A (en) | 1993-02-01 | 1993-02-01 | Apparatus for quantitative imaging of multiple fluorophores |
US08/189,190 US5418371A (en) | 1993-02-01 | 1994-01-31 | Apparatus for quantitative imaging of multiple fluorophores using dual detectors |
PCT/IB1994/000019 WO1994018547A1 (en) | 1993-02-01 | 1994-02-01 | Apparatus for quantitative imaging of multiple flurophores |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69402958D1 DE69402958D1 (de) | 1997-06-05 |
DE69402958T2 true DE69402958T2 (de) | 1997-12-11 |
Family
ID=26682894
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69402958T Expired - Fee Related DE69402958T2 (de) | 1993-02-01 | 1994-02-01 | Vorrichtung zur quantitativen abbildung von mehreren fluorophoren |
Country Status (6)
Country | Link |
---|---|
US (1) | US5418371A (de) |
EP (1) | EP0681695B1 (de) |
JP (1) | JPH08506419A (de) |
AT (1) | ATE152519T1 (de) |
DE (1) | DE69402958T2 (de) |
WO (1) | WO1994018547A1 (de) |
Cited By (8)
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DE10038526A1 (de) * | 2000-08-08 | 2002-02-28 | Zeiss Carl Jena Gmbh | Verfahren und Anordnung zur Erfassung des wellenlängenabhängigen Verhaltens einer beleuchteten Probe |
DE19829981C2 (de) * | 1998-07-04 | 2002-10-17 | Zeiss Carl Jena Gmbh | Verfahren und Anordnung zur konfokalen Mikroskopie |
DE10001954B4 (de) * | 1999-11-22 | 2004-07-22 | Leica Microsystems Heidelberg Gmbh | Laserscanmikroskop |
DE102005022880A1 (de) * | 2005-05-18 | 2006-11-30 | Olympus Soft Imaging Solutions Gmbh | Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern |
DE102010019095A1 (de) * | 2010-04-30 | 2011-11-03 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verfahren und Vorrichtung zur Fluoreszenzlebensdauermessung |
CN108801987A (zh) * | 2018-03-17 | 2018-11-13 | 杨佳苗 | 共焦分立荧光光谱及荧光寿命探测方法与装置 |
DE10339784B4 (de) | 2002-08-28 | 2021-09-16 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Mikroskopieverfahren |
DE102022122092A1 (de) | 2022-08-31 | 2024-02-29 | Pyroscience Gmbh | Referenz-Messung |
Families Citing this family (79)
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CH697814B1 (de) * | 2001-01-26 | 2009-02-27 | Tecan Trading Ag | Optisches System und Verfahren zum Anregen und Messen von Fluoreszenz an oder in mit Fluoreszenzfarbstoffen behandelten Proben. |
JP3645907B2 (ja) * | 1994-05-27 | 2005-05-11 | ノバルティス アクチエンゲゼルシャフト | 漸減励起された発光を検出する方法 |
US5735276A (en) * | 1995-03-21 | 1998-04-07 | Lemelson; Jerome | Method and apparatus for scanning and evaluating matter |
US5675155A (en) * | 1995-04-26 | 1997-10-07 | Beckman Instruments, Inc. | Multicapillary fluorescent detection system |
US5793049A (en) * | 1995-07-06 | 1998-08-11 | Yale University | Optical filtering and spectroscopic imaging |
DE19536296B4 (de) * | 1995-09-29 | 2004-10-14 | Daimlerchrysler Ag | Signalmarken und Verfahren zu deren Identifizierung |
US5784157A (en) * | 1995-11-21 | 1998-07-21 | The Research Foundation Of State University Of New York | Method and apparatus for identifying fluorophores |
ATE270437T1 (de) * | 1996-01-11 | 2004-07-15 | Univ Princeton | Organische luminiszenzbeschichtung fur lichtdetektoren |
AT403745B (de) * | 1996-02-29 | 1998-05-25 | Avl Verbrennungskraft Messtech | Messanordnung mit einem für anregungs- und messstrahlung transparentem trägerelement |
US5851488A (en) * | 1996-02-29 | 1998-12-22 | Biocircuits Corporation | Apparatus for automatic electro-optical chemical assay determination |
EP0814594B1 (de) * | 1996-06-18 | 2004-09-08 | Fuji Photo Film Co., Ltd. | Bildlesegerät |
DE19634873A1 (de) * | 1996-08-29 | 1998-03-12 | Boehringer Mannheim Gmbh | System zur Unterscheidung fluoreszierender Molekülgruppen durch zeitaufgelöste Fluoreszenzmessung |
DE19722630C2 (de) * | 1997-05-30 | 1999-09-16 | Stiftung Fuer Lasertechnologie | Verfahren zur in situ Messung mitochondrialer Stoffwechselprodukte |
US5986271A (en) * | 1997-07-03 | 1999-11-16 | Lazarev; Victor | Fluorescence imaging system |
US6469311B1 (en) | 1997-07-16 | 2002-10-22 | Molecular Devices Corporation | Detection device for light transmitted from a sensed volume |
US6097025A (en) * | 1997-10-31 | 2000-08-01 | Ljl Biosystems, Inc. | Light detection device having an optical-path switching mechanism |
US6071748A (en) * | 1997-07-16 | 2000-06-06 | Ljl Biosystems, Inc. | Light detection device |
GB9717021D0 (en) * | 1997-08-12 | 1997-10-15 | Kalibrant Limited | A detector |
US6576476B1 (en) | 1998-09-02 | 2003-06-10 | Ljl Biosystems, Inc. | Chemiluminescence detection method and device |
WO2000006990A2 (en) | 1998-07-27 | 2000-02-10 | Ljl Biosystems, Inc. | Apparatus and methods for time-resolved spectroscopic measurements |
WO2000006991A2 (en) | 1998-07-27 | 2000-02-10 | Ljl Biosystems, Inc. | Apparatus and methods for spectroscopic measurements |
US6992761B2 (en) * | 1997-09-20 | 2006-01-31 | Molecular Devices Corporation | Broad range light detection system |
US6297018B1 (en) | 1998-04-17 | 2001-10-02 | Ljl Biosystems, Inc. | Methods and apparatus for detecting nucleic acid polymorphisms |
US6326605B1 (en) | 1998-02-20 | 2001-12-04 | Ljl Biosystems, Inc. | Broad range light detection system |
WO2000050877A1 (en) | 1999-02-23 | 2000-08-31 | Ljl Biosystems, Inc. | Frequency-domain light detection device |
US6825921B1 (en) | 1999-11-10 | 2004-11-30 | Molecular Devices Corporation | Multi-mode light detection system |
US6091563A (en) * | 1997-09-26 | 2000-07-18 | Iomega Corporation | Latent illuminance discrimination marker system for data storage cartridges |
JP4435977B2 (ja) * | 1998-02-19 | 2010-03-24 | ライカ ミクロジュステムス ツェーエムエス ゲーエムベーハー | スペクトル選択素子を有する光学装置 |
US6528801B1 (en) * | 1998-11-04 | 2003-03-04 | The Research Foundation Of State University Of New York | Method and apparatus for detecting radiation |
US6455861B1 (en) * | 1998-11-24 | 2002-09-24 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
US6157037A (en) * | 1998-12-04 | 2000-12-05 | Photosense, Llc | Sensing device and method for measuring emission time delay during irradiation of targeted samples |
DE19936573A1 (de) * | 1998-12-22 | 2001-02-08 | Zeiss Carl Jena Gmbh | Anordnung zur Separierung von Anregungs- und Emissionslicht in einem Mikroskop |
US6320196B1 (en) | 1999-01-28 | 2001-11-20 | Agilent Technologies, Inc. | Multichannel high dynamic range scanner |
DE19915137C2 (de) * | 1999-03-26 | 2001-10-18 | Michael Schaefer | Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens |
EP1203218A1 (de) | 1999-07-30 | 2002-05-08 | California Institute of Technology | System und verfahren zur überwachung von zellulären aktivität |
US6329661B1 (en) * | 2000-02-29 | 2001-12-11 | The University Of Chicago | Biochip scanner device |
DE10038080A1 (de) * | 2000-08-04 | 2002-02-21 | Giesing Michael | Verfahren und Vorrichtung zum ortsaufgelösten fluoreszenzoptischen Nachweis von auf einer Oberfläche eines planaren Trägers immobilisierten Substanzen |
DE10054426B4 (de) * | 2000-10-27 | 2006-03-09 | Iom Innovative Optische Messtechnik Gmbh | Verfahren zur Multi-Fluoreszenz-Detektion |
DE10056384C2 (de) * | 2000-11-14 | 2003-06-05 | Leica Microsystems | Vorrichtung zur Messung der Lebensdauer eines angeregten Zustandes in einer Probe und Verwendung der Vorrichtung |
JP3999662B2 (ja) * | 2000-12-14 | 2007-10-31 | オリンパス株式会社 | 蛍光分析装置および蛍光分析方法 |
JP2002310894A (ja) * | 2001-04-11 | 2002-10-23 | Japan Science & Technology Corp | 発光観測装置、発光体選別装置、及び発光体の選別方法 |
DE10144435B4 (de) * | 2001-09-06 | 2005-03-24 | EuroPhoton GmbH Gesellschaft für optische Sensorik | Verfahren zur Charakterisierung der Eigenschaften von fluoreszierenden Proben, insbesondere lebenden Zellen und Geweben, in multi-well, in in-vitro Fluoreszenz-Assays, in DNA-Chips, Vorrichtungen zur Durchführung des Verfahrens und deren Verwendung |
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US20070096039A1 (en) * | 2002-05-03 | 2007-05-03 | Rakesh Kapoor | Evaluation Of Multicomponent Mixtures Using Modulated Light Beams |
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US7006547B2 (en) * | 2004-03-31 | 2006-02-28 | Cymer, Inc. | Very high repetition rate narrow band gas discharge laser system |
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Family Cites Families (6)
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---|---|---|---|---|
US4628026A (en) * | 1983-11-15 | 1986-12-09 | Dietlind Gardell | Method and apparatus for automated double fluorochromization analysis in lymphocytotoxicity testing |
US4937457A (en) * | 1989-02-10 | 1990-06-26 | Slm Instruments, Inc. | Picosecond multi-harmonic fourier fluorometer |
WO1992013265A1 (en) * | 1991-01-24 | 1992-08-06 | The University Of Maryland | Method and apparatus for multi-dimensional phase fluorescence lifetime imaging |
US5208651A (en) * | 1991-07-16 | 1993-05-04 | The Regents Of The University Of California | Apparatus and method for measuring fluorescence intensities at a plurality of wavelengths and lifetimes |
US5212386A (en) * | 1991-12-13 | 1993-05-18 | I.S.S. (U.S.A.) Inc. | High speed cross-correlation frequency domain fluorometry-phosphorimetry |
US5294799A (en) * | 1993-02-01 | 1994-03-15 | Aslund Nils R D | Apparatus for quantitative imaging of multiple fluorophores |
-
1994
- 1994-01-31 US US08/189,190 patent/US5418371A/en not_active Expired - Fee Related
- 1994-02-01 DE DE69402958T patent/DE69402958T2/de not_active Expired - Fee Related
- 1994-02-01 WO PCT/IB1994/000019 patent/WO1994018547A1/en active IP Right Grant
- 1994-02-01 EP EP94906355A patent/EP0681695B1/de not_active Expired - Lifetime
- 1994-02-01 JP JP6517853A patent/JPH08506419A/ja active Pending
- 1994-02-01 AT AT94906355T patent/ATE152519T1/de not_active IP Right Cessation
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0977069B2 (de) † | 1998-07-04 | 2017-03-15 | Carl Zeiss Microscopy GmbH | Verfahren und Anordnung zur konfokalen Mikroskopie |
DE19829981C2 (de) * | 1998-07-04 | 2002-10-17 | Zeiss Carl Jena Gmbh | Verfahren und Anordnung zur konfokalen Mikroskopie |
USRE41666E1 (en) | 1998-07-04 | 2010-09-14 | Carl Zeiss Jena Gmbh | Process and arrangement for confocal microscopy |
DE10001954B4 (de) * | 1999-11-22 | 2004-07-22 | Leica Microsystems Heidelberg Gmbh | Laserscanmikroskop |
DE10038526B4 (de) * | 2000-08-08 | 2004-09-02 | Carl Zeiss Jena Gmbh | Verfahren und Anordnung zur Erfassung des wellenlängenabhängigen Verhaltens einer beleuchteten Probe |
DE10038526A1 (de) * | 2000-08-08 | 2002-02-28 | Zeiss Carl Jena Gmbh | Verfahren und Anordnung zur Erfassung des wellenlängenabhängigen Verhaltens einer beleuchteten Probe |
DE10339784B4 (de) | 2002-08-28 | 2021-09-16 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Mikroskopieverfahren |
DE102005022880B4 (de) * | 2005-05-18 | 2010-12-30 | Olympus Soft Imaging Solutions Gmbh | Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern |
DE102005022880A1 (de) * | 2005-05-18 | 2006-11-30 | Olympus Soft Imaging Solutions Gmbh | Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern |
US8487275B2 (en) | 2010-04-30 | 2013-07-16 | Deutsches Zentrum fur-Luft-und Raumfahrt e.V. | Method and a device for measuring fluorescence lifetime |
DE102010019095B4 (de) * | 2010-04-30 | 2016-12-08 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verfahren und Vorrichtung zur Fluoreszenzlebensdauermessung |
DE102010019095A1 (de) * | 2010-04-30 | 2011-11-03 | Deutsches Zentrum für Luft- und Raumfahrt e.V. | Verfahren und Vorrichtung zur Fluoreszenzlebensdauermessung |
CN108801987A (zh) * | 2018-03-17 | 2018-11-13 | 杨佳苗 | 共焦分立荧光光谱及荧光寿命探测方法与装置 |
CN108801987B (zh) * | 2018-03-17 | 2021-03-23 | 杨佳苗 | 共焦分立荧光光谱及荧光寿命探测方法与装置 |
DE102022122092A1 (de) | 2022-08-31 | 2024-02-29 | Pyroscience Gmbh | Referenz-Messung |
Also Published As
Publication number | Publication date |
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EP0681695A1 (de) | 1995-11-15 |
EP0681695B1 (de) | 1997-05-02 |
WO1994018547A1 (en) | 1994-08-18 |
DE69402958D1 (de) | 1997-06-05 |
US5418371A (en) | 1995-05-23 |
ATE152519T1 (de) | 1997-05-15 |
JPH08506419A (ja) | 1996-07-09 |
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