DE69330329T2 - Klonierung und Charakterisierung des GHRH-Rezeptors - Google Patents

Klonierung und Charakterisierung des GHRH-Rezeptors

Info

Publication number
DE69330329T2
DE69330329T2 DE69330329T DE69330329T DE69330329T2 DE 69330329 T2 DE69330329 T2 DE 69330329T2 DE 69330329 T DE69330329 T DE 69330329T DE 69330329 T DE69330329 T DE 69330329T DE 69330329 T2 DE69330329 T2 DE 69330329T2
Authority
DE
Germany
Prior art keywords
ghrh
receptor
binding
seq
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69330329T
Other languages
English (en)
Other versions
DE69330329D1 (de
Inventor
Wayne American Cyanamid Co.
Charlottesville The University Of Virginia Patent Foundation
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UVA Licensing and Ventures Group
Original Assignee
University of Virginia UVA
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/946,232 external-priority patent/US5591641A/en
Priority claimed from US07/947,672 external-priority patent/US5612470A/en
Application filed by University of Virginia UVA, American Cyanamid Co filed Critical University of Virginia UVA
Publication of DE69330329D1 publication Critical patent/DE69330329D1/de
Application granted granted Critical
Publication of DE69330329T2 publication Critical patent/DE69330329T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Pathology (AREA)

Claims (12)

1. Isoliertes und gereinigtes Nukleinsäuremolekül, das für die Aminosäuresequenz SEQ ID NR: 8 codiert.
2. Isoliertes, gereinigtes Nukleinsäuremolekül der Nukleinsäuresequenz von SEQ ID NR: 7, das für einen Freisetzungshormonrezeptor für Wachstumshormon codiert.
3. Vektor umfassend ein Nukleinsäuremolekül der Sequenz von Anspruch 2.
4. Wirtszelle umfassend den Vektor von Anspruch 3.
5. Wirtszelle umfassend ein Nukleinsäuremolekül der Sequenz von Anspruch 2.
6. Isoliertes und gereinigtes Nukleinsäuremolektil, das für ein Polypeptid mit der biologischen Aktivität eines Freisetzungshormonrezeptors für Wachstumshormon der Aminosäuresequenz von SEQ ID NO: 8 codiert, wobei die Aktivität im Binden von Frei etzungshormon für Wachstumshormon besteht.
7. Expressionsvektor umfassend das Nukleinsäuremolekül von Anspruch 6, wobei das Nukleinsäuremolekül in einem Vektor vorliegt, der sich in einer prokaryotischen oder eukaryotischen Zelle vervielfältigen kann.
8. Escherichia coli umfassend den Expressionsvektor von Anspruch 7.
9. Affenzelte transformiert mit SV-40 umfassend den Expressionsvektor von Anspruch 7.
10. Verfahren zur rekombinanten Herstellung eines Freisetzungshormonrezeptors für Wachstumshormon der Aminosäuresequenz von SEQ ID NR: 8, umfassend die Transfektion einer Wirtzelle; mit dem Expressionsvektor von Anspruch 7, die Kultur der transfizierten Wirtszelle bei Bedingungen, die die Expression des Rezeptors ermöglichen sollten, und Gewinnung des Rezeptors.
11. Transfizierte DH5α Zellen hinterlegt bei der American Type Culture Collection unter der Zugriffsnummer ATCC 69058.
12. Isolierte und gereinigte DNA Sonde ausgewählt aus der Gruppe bestehend aus SEQ ID NR: 5 und SEQ ID NR: 6 zur Verwendung beim Screening von Nukleinsäuremolekülen, die; für Polypeptide codieren, welche Bindeaktivität für Freisetzungshormon für Wachstumshormon des Freisetzungshormonrezeptors für Wachstumshormon aufweisen.
DE69330329T 1992-09-15 1993-09-09 Klonierung und Charakterisierung des GHRH-Rezeptors Expired - Lifetime DE69330329T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/946,232 US5591641A (en) 1992-06-23 1992-09-15 Nucleic acid encoding the growth hormone releasing hormone receptor
US07/947,672 US5612470A (en) 1992-06-23 1992-09-18 Nucleic acid encoding the growth hormone releasing hormone receptor

Publications (2)

Publication Number Publication Date
DE69330329D1 DE69330329D1 (de) 2001-07-19
DE69330329T2 true DE69330329T2 (de) 2002-01-03

Family

ID=27130233

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69330329T Expired - Lifetime DE69330329T2 (de) 1992-09-15 1993-09-09 Klonierung und Charakterisierung des GHRH-Rezeptors

Country Status (12)

Country Link
EP (1) EP0591697B8 (de)
JP (1) JP3393159B2 (de)
KR (1) KR100342153B1 (de)
AT (1) ATE202141T1 (de)
AU (1) AU673188B2 (de)
DE (1) DE69330329T2 (de)
DK (1) DK0591697T3 (de)
ES (1) ES2159512T3 (de)
GR (1) GR3036486T3 (de)
IL (1) IL106860A (de)
NZ (2) NZ272688A (de)
PT (1) PT591697E (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2634926B2 (ja) * 1990-04-19 1997-07-30 シャープ株式会社 かな漢字変換装置
US5852187A (en) * 1992-06-23 1998-12-22 The University Of Virginia Patent Foundation Molecular cloning of the ovine pituitary growth hormone releasing hormone receptor
US5973114A (en) * 1992-06-23 1999-10-26 The University Of Virginia Patent Foundation Isolation and characterization of the growth hormone releasing hormone receptor
AU2003238032A1 (en) * 2002-06-24 2004-01-06 Bayer Healthcare Ag Regulation of human growth hormone-releasing hormone receptor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ238748A (en) * 1990-06-29 1993-09-27 Hoffmann La Roche Growth hormone releasing factor (grf) analogues
US5288621A (en) * 1990-12-14 1994-02-22 Cornell Research Foundation, Inc. Pituitary TRH receptor
US5225543A (en) * 1991-03-28 1993-07-06 American Cyanamid Company Receptors method for purification of g protein-linked
US5973114A (en) * 1992-06-23 1999-10-26 The University Of Virginia Patent Foundation Isolation and characterization of the growth hormone releasing hormone receptor
WO1994007915A1 (en) * 1992-09-28 1994-04-14 Northwestern University New growth hormone releasing hormone receptor protein

Also Published As

Publication number Publication date
EP0591697B8 (de) 2001-11-14
KR940007181A (ko) 1994-04-26
NZ248602A (en) 1996-06-25
DE69330329D1 (de) 2001-07-19
EP0591697B1 (de) 2001-06-13
DK0591697T3 (da) 2001-08-27
KR100342153B1 (ko) 2002-11-22
PT591697E (pt) 2001-11-30
IL106860A (en) 1999-08-17
JP3393159B2 (ja) 2003-04-07
EP0591697A3 (de) 1994-11-23
NZ272688A (en) 1997-07-27
ES2159512T3 (es) 2001-10-16
JPH0769916A (ja) 1995-03-14
AU4734093A (en) 1994-03-24
GR3036486T3 (en) 2001-11-30
IL106860A0 (en) 1993-12-08
AU673188B2 (en) 1996-10-31
ATE202141T1 (de) 2001-06-15
EP0591697A2 (de) 1994-04-13

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Owner name: THE UNIVERSITY OF VIRGINIA PATENT FOUNDATION, CHAR

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