DE69330329D1 - Klonierung und Charakterisierung des GHRH-Rezeptors - Google Patents

Klonierung und Charakterisierung des GHRH-Rezeptors

Info

Publication number
DE69330329D1
DE69330329D1 DE69330329T DE69330329T DE69330329D1 DE 69330329 D1 DE69330329 D1 DE 69330329D1 DE 69330329 T DE69330329 T DE 69330329T DE 69330329 T DE69330329 T DE 69330329T DE 69330329 D1 DE69330329 D1 DE 69330329D1
Authority
DE
Germany
Prior art keywords
ghrh
receptor
cloning
characterization
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69330329T
Other languages
English (en)
Other versions
DE69330329T2 (de
Inventor
Michael Oliver Thorner
Bruce David Gaylinn
John Ronald Zysk
Kevin Lynch
Jeffrey Keith Harrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Virginia Patent Foundation
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/946,232 external-priority patent/US5591641A/en
Priority claimed from US07/947,672 external-priority patent/US5612470A/en
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69330329D1 publication Critical patent/DE69330329D1/de
Publication of DE69330329T2 publication Critical patent/DE69330329T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Pathology (AREA)
DE69330329T 1992-09-15 1993-09-09 Klonierung und Charakterisierung des GHRH-Rezeptors Expired - Lifetime DE69330329T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/946,232 US5591641A (en) 1992-06-23 1992-09-15 Nucleic acid encoding the growth hormone releasing hormone receptor
US07/947,672 US5612470A (en) 1992-06-23 1992-09-18 Nucleic acid encoding the growth hormone releasing hormone receptor

Publications (2)

Publication Number Publication Date
DE69330329D1 true DE69330329D1 (de) 2001-07-19
DE69330329T2 DE69330329T2 (de) 2002-01-03

Family

ID=27130233

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69330329T Expired - Lifetime DE69330329T2 (de) 1992-09-15 1993-09-09 Klonierung und Charakterisierung des GHRH-Rezeptors

Country Status (12)

Country Link
EP (1) EP0591697B8 (de)
JP (1) JP3393159B2 (de)
KR (1) KR100342153B1 (de)
AT (1) ATE202141T1 (de)
AU (1) AU673188B2 (de)
DE (1) DE69330329T2 (de)
DK (1) DK0591697T3 (de)
ES (1) ES2159512T3 (de)
GR (1) GR3036486T3 (de)
IL (1) IL106860A (de)
NZ (2) NZ272688A (de)
PT (1) PT591697E (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2634926B2 (ja) * 1990-04-19 1997-07-30 シャープ株式会社 かな漢字変換装置
US5852187A (en) * 1992-06-23 1998-12-22 The University Of Virginia Patent Foundation Molecular cloning of the ovine pituitary growth hormone releasing hormone receptor
US5973114A (en) * 1992-06-23 1999-10-26 The University Of Virginia Patent Foundation Isolation and characterization of the growth hormone releasing hormone receptor
WO2004000883A1 (en) * 2002-06-24 2003-12-31 Bayer Healthcare Ag Regulation of human growth hormone-releasing hormone receptor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA914983B (en) * 1990-06-29 1992-03-25 Hoffmann La Roche His-grf-analogs
US5288621A (en) * 1990-12-14 1994-02-22 Cornell Research Foundation, Inc. Pituitary TRH receptor
US5225543A (en) * 1991-03-28 1993-07-06 American Cyanamid Company Receptors method for purification of g protein-linked
US5973114A (en) * 1992-06-23 1999-10-26 The University Of Virginia Patent Foundation Isolation and characterization of the growth hormone releasing hormone receptor
WO1994007915A1 (en) * 1992-09-28 1994-04-14 Northwestern University New growth hormone releasing hormone receptor protein

Also Published As

Publication number Publication date
EP0591697B1 (de) 2001-06-13
DE69330329T2 (de) 2002-01-03
KR940007181A (ko) 1994-04-26
ES2159512T3 (es) 2001-10-16
ATE202141T1 (de) 2001-06-15
KR100342153B1 (ko) 2002-11-22
JP3393159B2 (ja) 2003-04-07
AU4734093A (en) 1994-03-24
IL106860A (en) 1999-08-17
DK0591697T3 (da) 2001-08-27
NZ272688A (en) 1997-07-27
PT591697E (pt) 2001-11-30
EP0591697A2 (de) 1994-04-13
JPH0769916A (ja) 1995-03-14
GR3036486T3 (en) 2001-11-30
IL106860A0 (en) 1993-12-08
AU673188B2 (en) 1996-10-31
EP0591697A3 (de) 1994-11-23
EP0591697B8 (de) 2001-11-14
NZ248602A (en) 1996-06-25

Similar Documents

Publication Publication Date Title
EP0192392A3 (en) Insulin receptor, its purification, its production in recombinant cells, dna therefor, antibodies to insulin receptor and their preparation, and pharmaceutical compositions containing them
DE3854952T2 (de) Mykobakterielle rekombinanten und peptide
DE3686646T2 (de) Herstellung eines igg-bindenden proteins zum erleichtern der weiterverarbeitung mittels "protein-engineering".
EP0217919A4 (de) Typenspezifische papillomavirus-dns-sequenzen und peptide.
ES2009238A6 (es) Metodo para producir proteinas de mycobacterium tuberculosis y peptidos antigenicos de las mismas.
FI871919A (fi) Sekretionssignalselektionsvektorer foer extracellulaer proteinsyntes hos bacillus-bakterier.
DK0535216T3 (da) DNA. der koder mammale phosphodiesteraser
ATE91722T1 (de) Pferde-gamma-interferon.
IL88151A (en) Interleukin-7 protein, dna sequences encoding it, its preparation and compositions containing it
EP0192988A3 (en) Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom
ATE338113T1 (de) Transferrin-rezeptor gene von haemophilus
DE69330329T2 (de) Klonierung und Charakterisierung des GHRH-Rezeptors
CA2098972A1 (en) Isolation and characterization of the growth hormone releasing hormone receptor
DK27097A (da) Minactivin og fremgangsmåde til fremstilling deraf
RU2000111744A (ru) Очищенная и выделенная днк, химерный полинуклеотид, вектор экспрессии и клетка-хозяин
Baumbach et al. Nucleic acid molecule encoding the porcine growth hormone receptor
Thorner et al. Nucleic acid encoding the growth hormone releasing hormone receptor
ATE154074T1 (de) Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniae

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: THE UNIVERSITY OF VIRGINIA PATENT FOUNDATION, CHAR

8381 Inventor (new situation)

Free format text: ERFINDER IST ANMELDER

8364 No opposition during term of opposition