DE69009068D1 - Methoden und materialien zur expression von varianten von menschlichem plasminogen. - Google Patents

Methoden und materialien zur expression von varianten von menschlichem plasminogen.

Info

Publication number
DE69009068D1
DE69009068D1 DE69009068T DE69009068T DE69009068D1 DE 69009068 D1 DE69009068 D1 DE 69009068D1 DE 69009068 T DE69009068 T DE 69009068T DE 69009068 T DE69009068 T DE 69009068T DE 69009068 D1 DE69009068 D1 DE 69009068D1
Authority
DE
Germany
Prior art keywords
plasminogen
acylated
materials
methods
human plasminogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69009068T
Other languages
English (en)
Other versions
DE69009068T2 (de
Inventor
Francis Castellino
Deborah Higgins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DE69009068D1 publication Critical patent/DE69009068D1/de
Publication of DE69009068T2 publication Critical patent/DE69009068T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69009068T 1989-12-01 1990-10-31 Methoden und materialien zur expression von varianten von menschlichem plasminogen. Expired - Lifetime DE69009068T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/444,584 US5087572A (en) 1989-12-01 1989-12-01 Dna encoding human plasminogen modified at the cleavage site
PCT/US1990/006345 WO1991008297A1 (en) 1989-12-01 1990-10-31 Methods and materials for expression of human plasminogen variant

Publications (2)

Publication Number Publication Date
DE69009068D1 true DE69009068D1 (de) 1994-06-23
DE69009068T2 DE69009068T2 (de) 1994-10-27

Family

ID=23765514

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69009068T Expired - Lifetime DE69009068T2 (de) 1989-12-01 1990-10-31 Methoden und materialien zur expression von varianten von menschlichem plasminogen.

Country Status (14)

Country Link
US (1) US5087572A (de)
EP (1) EP0502872B1 (de)
JP (1) JP3043403B2 (de)
AT (1) ATE105862T1 (de)
AU (1) AU641449B2 (de)
CA (1) CA2072649C (de)
DE (1) DE69009068T2 (de)
DK (1) DK0502872T3 (de)
FI (1) FI102617B1 (de)
IE (1) IE62832B1 (de)
NO (1) NO307305B1 (de)
PL (1) PL288034A1 (de)
PT (1) PT96041A (de)
WO (1) WO1991008297A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
WO1992009893A1 (en) * 1990-11-29 1992-06-11 Carbaugh John E DIAGNOSTIC AND THERAPEUTIC COMPOSITIONS AND METHODS FOR LIPOPROTEIN(a)
JP3559559B2 (ja) 1992-06-03 2004-09-02 ジェネンテク，インコーポレイテッド 向上した治療特性を有する組織プラスミノーゲン活性化因子グリコシル化変異体
WO1994001128A1 (en) * 1992-07-01 1994-01-20 Beth Israel Hospital Boston Enhancement of thrombolytic therapy with deglycosylated plasminogen
US6048850A (en) * 1992-09-22 2000-04-11 Young; Donald A. Method of inhibiting prostaglandin synthesis in a human host
US5837479A (en) * 1992-09-22 1998-11-17 Young; Donald A. Screening assays for inhibitors of mammalian prostaglandin H synthase-2
GB9412131D0 (en) * 1994-06-17 1994-08-10 British Bio Technology Thrombolytic composition
US5919652A (en) * 1994-11-09 1999-07-06 The Regents Of The University Of California Nucleic acid molecules comprising the prostate specific antigen (PSA) promoter and uses thereof
WO2011004011A1 (en) 2009-07-10 2011-01-13 Thrombogenics Nv Variants of plasminogen and plasmin
KR20140015289A (ko) 2011-01-05 2014-02-06 쓰롬보제닉스 엔.브이. 플라스미노겐 및 플라스민 변이체
RU2604810C2 (ru) 2011-08-12 2016-12-10 Тромбодженикс Н.В. Варианты плазминогена и плазмина
JP2022540865A (ja) * 2019-07-12 2022-09-20 モナッシュ ユニバーシティ 組換えタンパク質を作製するための方法
AU2022209867A1 (en) * 2021-01-20 2023-08-17 Monash University Fusion proteins

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3943245A (en) * 1974-02-14 1976-03-09 Armour Pharmaceutical Company Purification of plasminogen
DE3065190D1 (en) * 1979-11-05 1983-11-10 Beecham Group Plc Enzyme derivatives, and their preparation
WO1986004351A1 (en) * 1985-01-25 1986-07-31 Sagami Chemical Research Center Stabilized human prourokinase
EP0201153A3 (de) * 1985-02-09 1987-10-07 Beecham Group Plc Modifiziertes Enzym und Verfahren zu dessen Herstellung
DE3682104D1 (de) * 1985-04-22 1991-11-28 Genentech Inc Plasminogenaktivator-mutante des menschlichen gewebes, verfahren und zwischenprodukte dafuer und diese mutante verwendende zusammensetzungen.
US5219569A (en) * 1985-04-22 1993-06-15 Genentech, Inc. Protease resistant urokinase
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
FI882407A (fi) * 1987-05-22 1988-11-23 Zymogenetics Inc Fibrinolytiska proteiner.
ATE118815T1 (de) * 1987-06-04 1995-03-15 Zymogenetics Inc Gewebs-plasminogen-aktivator-analoge mit modifizierten wachstumsfaktordomänen.
EP0293934B1 (de) * 1987-06-04 1994-08-31 Zymogenetics, Inc. Mutierter t-PA mit Kringle-Ersatz
WO1988010119A1 (en) * 1987-06-22 1988-12-29 Genetics Institute, Inc. Novel thrombolytic proteins

Also Published As

Publication number Publication date
JPH05502373A (ja) 1993-04-28
FI905919A0 (fi) 1990-11-30
DE69009068T2 (de) 1994-10-27
AU641449B2 (en) 1993-09-23
CA2072649A1 (en) 1991-06-02
FI102617B (fi) 1999-01-15
EP0502872A1 (de) 1992-09-16
PT96041A (pt) 1991-09-30
PL288034A1 (en) 1991-08-12
NO905211D0 (no) 1990-11-30
DK0502872T3 (da) 1994-09-12
FI905919A (fi) 1991-06-02
IE62832B1 (en) 1995-03-08
IE904332A1 (en) 1991-06-05
ATE105862T1 (de) 1994-06-15
CA2072649C (en) 2003-10-21
AU6721290A (en) 1991-06-26
JP3043403B2 (ja) 2000-05-22
EP0502872B1 (de) 1994-05-18
NO905211L (no) 1991-06-03
FI102617B1 (fi) 1999-01-15
WO1991008297A1 (en) 1991-06-13
US5087572A (en) 1992-02-11
NO307305B1 (no) 2000-03-13

Similar Documents

Publication Publication Date Title
ATE105862T1 (de) Methoden und materialien zur expression von varianten von menschlichem plasminogen.
ATE124452T1 (de) Gewebe-plasminogenaktivator mit zymogenen oder fibrinspezifischen eigenschaften.
PT87721A (pt) Novel proteins with factor viii activity process for their preparation using generically engineered cells and pharmaceutical compositions containing them
DE3682891D1 (de) Gewebeplasminogen-aktivator-mutant, dafuer kodierende rekombinante genetische information und verfahren zur herstellung dieser mutanten, deren verwendung und pharmazeutische zusammensetzungen.
ES8800347A1 (es) Metodo para preparar uroquinasa de una sola cadena resistente a proteasas
AU2036188A (en) New tissue plasminogen activator
HUT42524A (en) Process for the enhancement of the production of t-pa and scu-pa
ES2013500A6 (es) Metodo para producir una variante de secuencioa de aminoacidos de un activador de plasmionogeno.
FI915149A0 (fi) Foerfarande och material foer expression av humant plasminogen i ett eukaryotiskt cellsystem som saknar en laegesspecifik plasminogenaktivator.
ES2043610T3 (es) Procedimiento para la produccion de un polipeptido hibrido del tipo activador de plasminogeno.
PT83721B (pt) Processo de preparacao de composicoes farmaceuticas a base de activadores de plasminogenio com actividade trombolitica
HU886304D0 (en) Process for the production of new polypeptides
DE3852118D1 (de) Protease-ähnliche zytotoxische lymphozyten-spezifische moleküle und verfahren.
ATE114170T1 (de) Substitutionsvariante des gewebeplasminogenaktivators.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition