DE656950T1 - Tat-derivate transport polypeptide. - Google Patents

Tat-derivate transport polypeptide.

Info

Publication number
DE656950T1
DE656950T1 DE0656950T DE93920231T DE656950T1 DE 656950 T1 DE656950 T1 DE 656950T1 DE 0656950 T DE0656950 T DE 0656950T DE 93920231 T DE93920231 T DE 93920231T DE 656950 T1 DE656950 T1 DE 656950T1
Authority
DE
Germany
Prior art keywords
seq
amino acids
protein
fusion protein
cargo molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE0656950T
Other languages
English (en)
Inventor
James Barsoum
Stephen Fawell
R Pepinsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogen Inc
Original Assignee
Biogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Inc filed Critical Biogen Inc
Publication of DE656950T1 publication Critical patent/DE656950T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01007—Peroxidase (1.11.1.7), i.e. horseradish-peroxidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Claims (34)

EP 93920231.3-2105 BIOGEN, INC. u.Z.: H 713 EP g Patentansprüche Übersetzung nach Art. 67 EPÜ
1. Fusionsprotein, bestehend aus einer carboxyterminalen Frachtmoleküleinheit und einer aminoterminalen Trans-
2Q porteinheit, wobei
(a) die Transporteinheit gekennzeichnet ist durch:
(i) die Gegenwart der Aminosäuren 49-57 des HIV-
tat-Proteins,
(ii) die Abwesenheit der Aminosäuren 22-36 des HIV-tat-Proteins und
(iii) die Abwesenheit der Aminosäuren 73-86 des HIV-
tat-Proteins; und
(b) die Frachtmoleküleinheitt nach der Transporteinheitabhängigen intrazellulären Bereitstellung eine signifikante biologische Aktivität behält.
2. Fusionsprotein nach Anspruch 1, wobei die Frachtmoleküleinheit ausgewählt ist aus therapeutischen Molekülen, prophylaktischen Molekülen und diagnostischen Molekülen.
3. Fusionsprotein, bestehend aus einer carboxyterminalen
Frachtmoleküleinheit und einer aminoterminalen Transporteinheit, wobei die Frachtmoleküleinheit aus einem menschlichen Papillomavirus E2-Repressor besteht, der
„Φ seine biologische Aktivität nach der Bereitstellung in der Zielzelle behält, und die Transporteinheit ausgewählt ist aus:
(a) Aminosäuren 47-58 des HIV-tat-Proteins
(SEQ ID NO:47),
__ (b) Aminosäuren 47-72 des HIV-tat-Proteins
(SEQ ID N0:48),
(c) Aminosäuren 38-72 des HIV-tat-Proteins
(SEQ ID NO:49), und
s (d) Aminosäuren 38-58 des HIV-tat-Proteins (SEQ ID NO:50).
4. Fusionsprotein nach Anspruch 3, wobei der Transporteinheit ein aminoterminales Methionin vorausgeht.
5. Fusionsprotein nach einem der Ansprüche 1 bis 4, wobei die Frachtmoleküleinheit aus den Aminosäuren 245-365 des menschlichen Papillomavirus E2-Proteins (SEQ ID NO:51) besteht.
6. Fusionsprotein JB106 (SEQ ID NO:38).
7. Fusionsprotein JB117 (SEQ ID NO:59). 15
8. Fusionsprotein JB118 (SEQ ID NO:60).
9. Fusionsprotein JB122 (SEQ ID NO:63).
10. Fusxonsprotein, bestehend aus einer carboxyterminalen
Frachtmoleküleinheit und einer aminoterminalen Transporteinheit, wobei die Frachtmoleküleinheit aus einem Rinder-Papillomavirus E2-Repressor besteht, der seine biologische Aktivität nach Bereitstellung in einer Zielzelle behält, und die Transporteinheit ausgewählt ist aus:
(a) Aminosäuren 47-62 des HIV-tat-Proteins
(SEQ ID NO:52) und
(b) Aminosäuren 38-62 des HIV-tat-Proteins (SEQ ID NO:53).
11. Fusionsprotein nach Anspruch 10, wobei der Transporteinheit ein aminoterminales Methionin vorausgeht.
;
12. Fusionsprotein nach einem der Ansprüche 1, 2, 10 oder 11, wobei die Frachtmoleküleinheit ein E2-Repressor ist, der aus den Aminosäuren 250-410 des Rinder-Papillomavi-
rus E2-Proteins (SEQ ID NO:56) besteht. 5
13. Fusionsprotein JB119 (SEQ ID NO:61).
14. Fusionsprotein JB120 (SEQ ID NO:62).
15. Kovalent verknüpftes chemisches Konjugat, bestehend aus einer Transportpolypeptid-Einheit und einer Frachtmoleküleinheit, wobei:
(a) die Transportpolypeptid-Einheit des Konjugats gekennzeichnet ist durch:
(i) die Gegenwart der Aminosäuren 49-57 des HIV-
tat-Proteins,
(ii) die Abwesenheit der Aminosäuren 22-36 des HIV-
tat-Proteins und
(iii) die Abwesenheit der Aminosäuren 73-86 des HIV-tat-Proteins, und
(b) die Frachtmoleküleinheit des Konjugats nach der Transporteinheit-abhängigen intrazellulären Bereitstellung eine signifikante biologische Aktivität behält.
16. Kovalent verknüpftes chemisches Konjugat nach Anspruch
15, wobei die Transportpolypeptid-Einheit aus den Aminosäuren 37-72 des HIV-tat-Proteins (SEQ ID NO:2) besteht.
17. Kovalent verknüpftes chemisches Konjugat nach Anspruch
16, wobei die Frachtmoleküleinheit ausgewählt ist aus:
(a) Aminosäuren 245-365 des menschlichen Papillomavirus E2-Proteins (SEQ ID-NO:51); und
(b) Aminosäuren 245-3 65 des menschlichen Papillomavirus E2-Proteins, wobei die Aminosäuren 300 und 309 zu Cystein geändert wurden (SEQ ID NO:55).
18. Kovalent verknüpftes chemisches Konjugat, bestehend aus einer Transporteinheit und einer Frachtmoleküleinheit, wobei das Transportpolypeptid aus den Aminosäuren 37-72 des HIV-tat-Proteins (SEQ ID NO:2) besteht und die Frachtmoleküleinheit ausgewählt ist aus:
(a) Aminosäuren 245-365 des menschlichen Papillomavirus E2-Proteins (SEQ ID NO:51) und
(b) Aminosäuren 245-365 des menschlichen Papillomavirus E2-Proteins, wobei die Aminosäuren 3 00 und 309 zu Cystein geändert wurden (SEQ ID NO:55).
19. Fusionsprotein, bestehend aus einer carboxyterminalen Frachtmoleküleinheit und einer aminoterminalen Transporteinheit, wobei die Frachtmoleküleinheit aus den Ami-
nosäuren 43-412 des HSV-VP16-Proteins besteht und die Transporteinheit aus den Aminosäuren 47-58 des HIV-tat-Proteins besteht.
20. Fusionsprotein nach Anspruch 19, wobei der Transporteinheit ein aminoterminales Methionin vorausgeht.
21. Kovalent verknüpftes chemisches Konjugat, bestehend aus einer Transportpolypeptid-Einheit und einer Frachtmoleküleinheit, wobei die Transportpolypeptid-Einheit aus
den Aminosäuren 37-72 des HIV-tat-Proteins (SEQ ID N0:2) besteht und die Frachtmoleküleinheit eine doppelsträngige DNA ist, ausgewählt aus:
(a) Oligonucleotid NFl (SEQ ID NO:43) angelagert an Oli-
gonucleotid NF2 (SEQ ID N0:44), und
(b) Oligonucleotid NF3 (SEQ ID NO:45) angelagert an Oligonucleotid NF4 (SEQ ID NO:46).
22. Verwendung eines Fusionsproteins nach einem der Ansprüche 1 bis 14, 19 oder 20 für die intrazelluläre Bereit-Stellung eines Frachtmoleküls.
23. Verwendung eines !covalent verknüpften chemischen Konjugats nach einem der Ansprüche 15 bis 17 oder 21 für die intrazelluläre Bereitstellung eines Frachtmoleküls.
24. Arzneimittel, umfassend eine pharmazeutisch wirksame Menge eines Fusionsproteins nach einem der Ansprüche 1 bis 14.
25. Arzneimittel, umfassend eine pharmazeutisch wirksame Menge eines Fusionsproteins nach Anspruch 19 oder 20.
26. Arzneimittel, umfassend eine pharmazeutisch wirksame Menge eines kovalent verknüpften chemischen Konjugats
nach einem der Ansprüche 15 bis 18 oder 21.
15
27. DNA-Molekül, umfassend eine Nucleotidsequenz, die ein Fusionsprotein codiert, ausgewählt aus:
(a) JB106 (SEQ ID NO:38),
(b) JB117 (SEQ ID NO:59),
(c) JB118 (SEQ ID NO:60),
(d) JB119 (SEQ ID NO:61),
(e) JB120 (SEQ ID NO:62), und
(f) JB122 (SEQ ID NO:63).
28. DNA-Molekül, umfassend eine Nucleotidsequenz, die das Fusionsprotein tat-VP16R.GF (SEQ ID NO:58) codiert.
29. DNA-Molekül nach Anspruch 27, wobei die das Fusionsprotein codierende Nucleotidsequenz funktionell mit Expres-
° sionskontrollsequenzen verknüpft ist.
30. DNA-Molekül nach Anspruch 28, wobei die das Fusionsprotein codierende Nucleotidsequenz funktionell mit Expressionskontroll Sequenzen verknüpft ist.
31. Einzelliger Wirt, transformiert mit einem DNA-Molekül nach Anspruch 29.
32. Einzelliger Wirt, transformiert mit einem DNA-Molekül nach Anspruch. 30.
33. Verfahren zur Herstellung eines Fusionsproteins, ausgewählt aus:
(a) JB106 (SEQ IDNO.-38),
(b) JB117 (SEQ ID NO:59), (C) JB118 (SEQ IDNO:60),
(d) JB119 (SEQ ID NO:61),
(e) JB120 (SEQ ID NO:62), und
(f) JB122 (SEQ IDNO:63),
wobei das Verfahren folgende Schritte umfaßt:
(a) Züchtung eines transformierten einzelligen Wirts
nach Anspruch 31 und
(b) Gewinnung des Fusionsproteins aus der Kultur.
34. Verfahren zur Herstellung eines Fusionsproteins, das aus den Aminosäuren 47-58 des HIV-tat-Proteins gefolgt von den Aminosäuren 43-412 des HSV-VP16-Proteins besteht, wobei das Verfahren folgende Schritte umfaßt:
(a) Züchtung eines transformierten einzelligen Wirts nach Anspruch 32 und
(b) Gewinnung des Fusionsproteins aus der Kultur. 25
DE0656950T 1992-08-21 1993-08-19 Tat-derivate transport polypeptide. Pending DE656950T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93437592A 1992-08-21 1992-08-21
PCT/US1993/007833 WO1994004686A1 (en) 1992-08-21 1993-08-19 Tat-derived transport polypeptides

Publications (1)

Publication Number Publication Date
DE656950T1 true DE656950T1 (de) 1996-03-14

Family

ID=25465458

Family Applications (2)

Application Number Title Priority Date Filing Date
DE0656950T Pending DE656950T1 (de) 1992-08-21 1993-08-19 Tat-derivate transport polypeptide.
DE69321962T Expired - Lifetime DE69321962T2 (de) 1992-08-21 1993-08-19 Von tat abgeleitete transportpolypeptide

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69321962T Expired - Lifetime DE69321962T2 (de) 1992-08-21 1993-08-19 Von tat abgeleitete transportpolypeptide

Country Status (14)

Country Link
EP (3) EP2000536A3 (de)
JP (2) JP2702285B2 (de)
KR (1) KR0153027B1 (de)
AT (1) ATE173016T1 (de)
AU (1) AU667244B2 (de)
CA (1) CA2135642C (de)
DE (2) DE656950T1 (de)
DK (1) DK0656950T3 (de)
ES (1) ES2123062T3 (de)
FI (1) FI120495B (de)
HK (1) HK1012678A1 (de)
NO (1) NO316761B1 (de)
NZ (1) NZ255831A (de)
WO (1) WO1994004686A1 (de)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219990A (en) * 1991-01-28 1993-06-15 Biogen, Inc. Papillomavirus e2 trans-activation repressors
US5861161A (en) 1994-09-07 1999-01-19 Universite De Montreal Chimeric proteins comprising a Vpr/Vpx virion incorporation domain for targeting into HIV-1 or HIV-2 virions
US5955593A (en) * 1996-09-09 1999-09-21 Washington University BH3 interacting domain death agonist
US6623938B2 (en) * 1997-01-21 2003-09-23 Human Genome Sciences, Inc. I-flice, a novel inhibitor of tumor necrosis factor receptor-1 and CD-95 induced apoptosis
ES2210761T3 (es) 1997-05-21 2004-07-01 The Board Of Trustees Of The Leland Stanford Junior University Composicion y procedimiento para mejorar el transporte a traves de las membranas biologicas.
DE69819150T3 (de) 1997-06-06 2007-12-20 Dynavax Technologies Corp., San Diego Immunstimulierende oligonucleotide, zusammensetzungen davon, und verfahren zur verwendung davon
GB9720585D0 (en) * 1997-09-26 1997-11-26 Smithkline Beecham Biolog Vaccine
AU755564B2 (en) * 1998-06-20 2002-12-12 Washington University Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy
US8038984B2 (en) 1998-06-20 2011-10-18 Washington University Membrane-permeant peptide complexes for treatment of sepsis
US6589503B1 (en) 1998-06-20 2003-07-08 Washington University Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy
US7306784B2 (en) 1998-06-20 2007-12-11 Washington University Membrane-permeant peptide complexes for medical imaging, diagnostics, and pharmaceutical therapy
AU762006B2 (en) * 1998-12-24 2003-06-19 Ucb Peptidic product, process and composition
DE19916224C1 (de) 1999-04-10 2000-06-21 November Ag Molekulare Medizin Synthetisches biologisch aktives Molekül
WO2001013957A2 (en) 1999-08-24 2001-03-01 Cellgate, Inc. Enhancing drug delivery across and into epithelial tissues using oligo arginine moieties
US7229961B2 (en) 1999-08-24 2007-06-12 Cellgate, Inc. Compositions and methods for enhancing drug delivery across and into ocular tissues
US6669951B2 (en) 1999-08-24 2003-12-30 Cellgate, Inc. Compositions and methods for enhancing drug delivery across and into epithelial tissues
US6730293B1 (en) 1999-08-24 2004-05-04 Cellgate, Inc. Compositions and methods for treating inflammatory diseases of the skin
WO2001015511A2 (en) * 1999-09-01 2001-03-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, dna and viruses
US20030108539A1 (en) * 2000-02-14 2003-06-12 Christophe Bonny Cell-permeable peptide inhibitors of the JNK signal transduction pathway
US6610820B1 (en) * 1999-10-12 2003-08-26 University Of Lausanne Cell-permeable peptide inhibitors of the JNK signal transduction pathway
US8183339B1 (en) 1999-10-12 2012-05-22 Xigen S.A. Cell-permeable peptide inhibitors of the JNK signal transduction pathway
US20040082509A1 (en) 1999-10-12 2004-04-29 Christophe Bonny Cell-permeable peptide inhibitors of the JNK signal transduction pathway
CA2396149A1 (en) * 2000-01-07 2001-07-12 Artemis Pharmaceuticals Gmbh Transduction of recombinases for inducible gene targeting
DK2364734T3 (en) * 2000-07-21 2017-10-30 Revance Therapeutics Inc Biological multicomponent transport systems
KR100379577B1 (ko) * 2000-07-26 2003-04-08 최수영 세포침투성 티에이티 - 인간 글루탐산 탈수소효소 융합단백질, 이 융합단백질을 코딩하는 재조합폴리뉴클레오타이드, 이 융합단백질의 발현벡터 및 이융합단백질을 세포 내로 도입하는 방법
KR100379578B1 (ko) * 2000-07-26 2003-04-08 최수영 세포침투성 티에이티-씨3 트랜스페라제 융합단백질, 이융합단백질의 발현벡터 및 티에이티-씨3 트랜스페라제를이용한 로 단백질의 생리적 기능 분석방법
EP2149604A1 (de) 2000-09-08 2010-02-03 Universität Zürich Entnahme von Repeat-Proteinen mit Repeat-Modulen
US20050059031A1 (en) 2000-10-06 2005-03-17 Quantum Dot Corporation Method for enhancing transport of semiconductor nanocrystals across biological membranes
CA2437983C (en) 2001-02-16 2011-10-25 Cellgate, Inc. Transporters comprising spaced arginine moieties
JP4130955B2 (ja) * 2001-06-05 2008-08-13 真寛 平岡 有酸素条件下の細胞内でタンパク質を不安定化させるポリペプチドとそれをコードするｄｎａ
KR100495138B1 (ko) * 2001-07-31 2005-06-14 학교법인 한림대학교 식물세포 투과 도메인-화물분자 복합체
WO2003025220A2 (en) 2001-09-18 2003-03-27 Carnegie Institution Of Washington Fusion proteins useful for detecting analytes
AU2002356824A1 (en) * 2001-10-19 2003-05-06 The Scripps Research Institute Novel methods for introducing molecules into cells and vectors and compositions for use in such methods
JP2005525096A (ja) 2002-01-09 2005-08-25 ユニバーシティ オブ ローザンヌ Ｊｎｋシグナル伝達経路の細胞浸透性ペプチドインヒビター
US7026166B2 (en) 2002-01-22 2006-04-11 Chiron Corporation Fluorogenic dyes
WO2003086470A2 (en) * 2002-04-17 2003-10-23 Deutsches Krebsforschungszentrum Smac-peptides as therapeutics against cancer and autoimmune diseases
EP1354953A1 (de) * 2002-04-17 2003-10-22 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Smac-Peptide zur Verwendung als therapeutische Mittel gegen Krebs und Autoimmunerkrankungen
US7459424B2 (en) 2002-04-22 2008-12-02 The Borad Of Trustees Of The Leland Stanford Junior University Peptide inhibitors of protein kinase C γ for pain management
KR100472939B1 (ko) * 2002-05-24 2005-03-18 학교법인 한림대학교 세포침투성 티에이티-피리독살 카이네이즈 융합단백질 및그 용도
AU2003254066B2 (en) 2002-07-19 2009-08-27 The Regents Of The University Of California Dendrimers as molecular translocators
KR100472938B1 (ko) * 2002-10-31 2005-03-11 학교법인 한림대학교 세포침투 효율을 향상시킨 수송도메인-목표단백질-수송도메인 융합단백질 및 그 용도
US7704756B2 (en) 2003-01-21 2010-04-27 Novartis Vaccines And Diagnostics, Inc. Fluorogenic dyes
US7468418B2 (en) 2003-04-29 2008-12-23 Avi Biopharma., Inc. Compositions for enhancing transport of molecules into cells
JPWO2005035562A1 (ja) * 2003-10-07 2007-11-22 伸弥 山中 新規な細胞増殖促進剤
US7700737B2 (en) 2003-12-05 2010-04-20 Multimmune Gmbh Therapeutic and diagnostic anti-Hsp70 antibodies
US8974774B2 (en) 2004-03-03 2015-03-10 Revance Therapeutics, Inc. Compositions and methods for topical diagnostic and therapeutic transport
US9211248B2 (en) 2004-03-03 2015-12-15 Revance Therapeutics, Inc. Compositions and methods for topical application and transdermal delivery of botulinum toxins
US20050288246A1 (en) 2004-05-24 2005-12-29 Iversen Patrick L Peptide conjugated, inosine-substituted antisense oligomer compound and method
JP4474264B2 (ja) * 2004-08-20 2010-06-02 生寶生物科技股▲ふん▼有限公司 子宮頸癌抑制の融合蛋白
WO2006032436A2 (en) 2004-09-21 2006-03-30 Nascacell Technologies Ag. Use of microproteins as tryptase inhibitors
JP4596391B2 (ja) 2005-02-10 2010-12-08 国立大学法人大阪大学 細胞透過性ペプチド
EP1988167B1 (de) * 2005-02-17 2020-04-01 Sorbonne Universite Intrazellular hemmende Peptide
NZ560799A (en) 2005-03-03 2009-10-30 Revance Therapeutics Inc Compositions and methods for topical application and transdermal delivery of an oligopeptide
ZA200707352B (en) 2005-03-03 2009-04-29 Revance Therapeutics Inc Compositions and methods for topical application and transdermal delivery of botulinum toxins
EP1861417B1 (de) 2005-03-10 2013-05-15 BioNTech AG Dimere oder multimere mikroproteine
US7973084B2 (en) 2005-04-28 2011-07-05 Postech Academy-Industrial Foundation Molecular transporters based on alditol or inositol and processes for the preparation thereof
KR100699279B1 (ko) * 2005-04-28 2007-03-23 학교법인 포항공과대학교 당 또는 당 유사체를 골격으로 하는 분자 수송체 및 그의제조방법
US8067571B2 (en) 2005-07-13 2011-11-29 Avi Biopharma, Inc. Antibacterial antisense oligonucleotide and method
US8080517B2 (en) 2005-09-12 2011-12-20 Xigen Sa Cell-permeable peptide inhibitors of the JNK signal transduction pathway
WO2007031098A1 (en) 2005-09-12 2007-03-22 Xigen S.A. Cell-permeable peptide inhibitors of the jnk signal transduction pathway
IN2014DN06624A (de) 2005-10-18 2015-07-10 Univ Colorado
US7517959B2 (en) 2005-11-11 2009-04-14 Walter And Eliza Hall Institute Of Medical Research SOCS3 proteins
PT2099826E (pt) 2007-01-05 2014-01-09 Univ Zuerich Anticorpo anti-beta-amilóide e suas utilizações
CA2680914C (en) 2007-03-13 2019-06-18 University Of Zurich Monoclonal human tumor-specific antibody
CN101688228B (zh) 2007-03-13 2014-12-24 国家犾太健康中心 抗体产生的方法
JP2010531666A (ja) 2007-06-26 2010-09-30 ユニバーシティ オブ マイアミ 抗体−エンドスタチン融合タンパク質及びそのバリアント
US20100016215A1 (en) 2007-06-29 2010-01-21 Avi Biopharma, Inc. Compound and method for treating myotonic dystrophy
KR101722038B1 (ko) 2007-07-26 2017-04-03 레반스 테라퓨틱스, 아이엔씨. 항미생물 펩티드, 조성물, 및 이용 방법
EP2527369A3 (de) 2007-09-13 2012-12-19 University Of Zurich Prorektorat Forschung Monoklonaler amyloid-beta (abeta)-spezifischer Antikörper und seine Verwendung
US7935816B2 (en) 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
WO2009107766A1 (ja) 2008-02-28 2009-09-03 東レ株式会社 経鼻投与用医薬組成物
CA2723114C (en) 2008-05-16 2018-02-27 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
WO2009143864A1 (en) 2008-05-30 2009-12-03 Xigen S.A. Use of cell-permeable peptide inhibitors of the jnk signal transduction pathway for the treatment of chronic or non-chronic inflammatory digestive diseases
WO2009143865A1 (en) 2008-05-30 2009-12-03 Xigen S.A. Use of cell-permeable peptide inhibitors of the jnk signal transduction pathway for the treatment of various diseases
CN102083970B (zh) 2008-07-21 2014-10-22 泰加生物工艺学公司 分化无核细胞及其制备方法
CA2735710A1 (en) 2008-08-04 2010-02-11 Glen N. Barber Sting (stimulator of interferon genes), a regulator of innate immune responses
DK2966084T3 (en) 2008-08-28 2018-08-06 Taiga Biotechnologies Inc MODULATORS OF MYC, PROCEDURES FOR USING SAME AND PROCEDURES FOR IDENTIFYING SUBSTANCES MODULATING MYC
WO2010072228A1 (en) 2008-12-22 2010-07-01 Xigen S.A. Novel transporter constructs and transporter cargo conjugate molecules
ES2546304T3 (es) 2009-05-20 2015-09-22 Toray Industries, Inc. Péptidos permeables para la membrana celular
EP2488210A4 (de) 2009-10-12 2014-04-30 Smith Holdings Llc Verfahren und zusammensetzungen zur modulation der genexpression mit in-vivo oder in-vitro verabreichten arzneistoffen auf oligonukleotidbasis
WO2011160653A1 (en) 2010-06-21 2011-12-29 Xigen S.A. Novel jnk inhibitor molecules
CN105457016B (zh) 2010-09-22 2022-06-24 科罗拉多大学董事会 Smad7的治疗应用
CA2807036C (en) 2010-10-14 2018-01-16 Xigen S.A. Use of cell-permeable peptide inhibitors of the jnk signal transduction pathway for the treatment of chronic or non-chronic inflammatory eye diseases
JP5828909B2 (ja) 2011-01-10 2015-12-09 シーティー アトランティック リミテッド 腫瘍関連抗原結合抗体を含む併用療法
CA3092114A1 (en) 2011-05-05 2012-11-08 Sarepta Therapeutics, Inc. Peptide oligonucleotide conjugates
US9161948B2 (en) 2011-05-05 2015-10-20 Sarepta Therapeutics, Inc. Peptide oligonucleotide conjugates
EP2714742A1 (de) 2011-06-03 2014-04-09 CT Atlantic Ltd. Magea3-bindende antikörper
EP2714743A1 (de) 2011-06-03 2014-04-09 CT Atlantic Ltd. Magea3-bindende antikörper
DE202011103324U1 (de) 2011-07-12 2012-01-02 Nekonal S.A.R.L. Therapeutische anti-TIRC7 Antikörper für die Verwendung in Immun und anderen Krankheiten
WO2013091670A1 (en) 2011-12-21 2013-06-27 Xigen S.A. Novel jnk inhibitor molecules for treatment of various diseases
US20150030602A1 (en) 2011-12-23 2015-01-29 Phenoquest Ag Antibodies for the treatment and diagnosis of affective and anxiety disorders
SI2797952T1 (sl) 2011-12-28 2019-07-31 Immunoqure Ag Postopek za pripravo monoklonskih avto-protiteles z želeno specifičnostjo
US9789135B2 (en) 2012-07-20 2017-10-17 Taiga Biotechnologies, Inc. Enhanced reconstitution and autoreconstitution of the hematopoietic compartment
US10456448B2 (en) 2013-03-08 2019-10-29 The Regents Of The University Of Colorado, A Body Corporate PTD-SMAD7 therapeutics
US10272115B2 (en) 2013-03-11 2019-04-30 Taiga Biotechnologies, Inc. Production and use of red blood cells
US9365825B2 (en) 2013-03-11 2016-06-14 Taiga Biotechnologies, Inc. Expansion of adult stem cells in vitro
US10981961B2 (en) 2013-03-11 2021-04-20 University Of Florida Research Foundation, Incorporated Delivery of card protein as therapy for occular inflammation
PL3013353T3 (pl) * 2013-06-26 2021-09-20 Xigen Inflammation Ltd. Przenikające do komórki inhibitory peptydowe szlaku przekazywania sygnału jnk do leczenia zapalenia pęcherza
WO2014206427A1 (en) 2013-06-26 2014-12-31 Xigen Inflammation Ltd. New use of cell-permeable peptide inhibitors of the jnk signal transduction pathway for the treatment of various diseases
WO2015197097A1 (en) 2014-06-26 2015-12-30 Xigen Inflammation Ltd. New use for jnk inhibitor molecules for treatment of various diseases
WO2015001013A2 (en) 2013-07-03 2015-01-08 Immunoqure Ag Human anti-ifn-alpha antibodies
US11053291B2 (en) 2014-02-19 2021-07-06 University Of Florida Research Foundation, Incorporated Delivery of Nrf2 as therapy for protection against reactive oxygen species
EP3116898B1 (de) 2014-03-11 2022-01-26 University of Florida Research Foundation, Inc. Aav-exprimiertes m013-protein als entzündungshemmendes therapeutikum in einer methode zur behandlung von augenkrankheiten
JP6468722B2 (ja) * 2014-04-30 2019-02-13 日本信号株式会社 物体検出装置
US11292838B2 (en) 2015-06-01 2022-04-05 Medigene Immunotherapies Gmbh Method for generating antibodies against T cell receptor
WO2016193299A1 (en) 2015-06-01 2016-12-08 Medigene Immunotherapies Gmbh T cell receptor library
KR20180020202A (ko) 2015-06-01 2018-02-27 메디진 이뮤노테라피스 게엠바하 T 세포 수용체 특이적 항체
WO2016196897A1 (en) 2015-06-04 2016-12-08 Sarepta Therapeutics, Inc. Methods and compounds for treatment of lymphocyte-related diseases and conditions
EP3309178A4 (de) 2015-06-11 2019-05-08 Keio University Fusionsprotein oder konjugiertes protein, träger zur intrazellulären abgabe, teilpeptid, zellmembran-permeationsverstärker, dna und vektor
KR20200100858A (ko) 2015-12-23 2020-08-26 메디진 이뮤노테라피스 게엠바하 수지상 세포 조성물
KR20190092472A (ko) 2016-12-02 2019-08-07 타이가 바이오테크놀로지스, 인코포레이티드 나노입자 제제
PT3554553T (pt) 2016-12-19 2022-08-04 Sarepta Therapeutics Inc Conjugados oligoméricos de salto de exão para a distrofia muscular
US10149898B2 (en) 2017-08-03 2018-12-11 Taiga Biotechnologies, Inc. Methods and compositions for the treatment of melanoma
JP7489319B2 (ja) 2018-09-26 2024-05-23 株式会社カネカ 細胞膜透過性ペプチド
WO2022154079A1 (ja) * 2021-01-18 2022-07-21 国立研究開発法人産業技術総合研究所 タンパク質を動物細胞に導入する方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05505102A (ja) * 1989-12-21 1993-08-05 ホワイトヘツド・インスチチユート・フオー・バイオメデイカル・リサーチ 真核細胞の中に分子を配達する方法
US5219990A (en) 1991-01-28 1993-06-15 Biogen, Inc. Papillomavirus e2 trans-activation repressors

Also Published As

Publication number Publication date
EP2000536A3 (de) 2010-06-30
CA2135642A1 (en) 1994-03-03
AU5083293A (en) 1994-03-15
DE69321962D1 (de) 1998-12-10
CA2135642C (en) 1999-12-14
NZ255831A (en) 1997-04-24
JP2702285B2 (ja) 1998-01-21
EP0903408A2 (de) 1999-03-24
AU667244B2 (en) 1996-03-14
NO316761B1 (no) 2004-05-03
DK0656950T3 (da) 1999-07-19
EP2000536A2 (de) 2008-12-10
FI120495B (fi) 2009-11-13
ATE173016T1 (de) 1998-11-15
ES2123062T3 (es) 1999-01-01
KR0153027B1 (en) 1998-10-15
JP2869396B2 (ja) 1999-03-10
DE69321962T2 (de) 1999-07-01
NO944273L (no) 1995-02-17
NO944273D0 (no) 1994-11-09
EP0656950A1 (de) 1995-06-14
FI945248A0 (fi) 1994-11-08
WO1994004686A1 (en) 1994-03-03
EP0903408A3 (de) 2005-11-02
FI945248A (fi) 1995-01-05
JPH1033186A (ja) 1998-02-10
JPH07503617A (ja) 1995-04-20
HK1012678A1 (en) 1999-08-06
EP0656950B1 (de) 1998-11-04

Similar Documents

Publication Publication Date Title
DE656950T1 (de) Tat-derivate transport polypeptide.
JP2766999B2 (ja) プロペプチド
DE3382742T2 (de) Ein Consensus-Leukozyten-Interferon und ein dafür kodierendes Gen.
DE68928532T2 (de) Funktionelles, durch ein rekombinantes verfahren hergestelltes synthetisches proteinpolymer
Eckert et al. Topology of the transposon Tn10-encoded tetracycline resistance protein within the inner membrane of Escherichia coli
CN112552393B (zh) 一种重组人源iii型胶原蛋白及其毕赤酵母重组表达系统
DE3882593T2 (de) Polypeptid und dessen Herstellung.
KR890012000A (ko) 인간 b세포 자극인자-2에 대한 수용체 단백질
DE68914664T2 (de) Gereinigte ubiquitin-hydrolase, diese kodierende dns-sequenzen und ihre verwendung zur gewinnung von polypeptiden.
CN114805551B (zh) 一种重组iii型胶原蛋白及其制备方法
DE69125810T2 (de) Verfahren zur enzymatischen herstellung von basischen fibroblasten-wachstumsfaktors
IE61050B1 (en) Polypeptides
DD218118A5 (de) Verfahren zur herstellung eines hefe- oder nichthefepolypeptids
DE69314574T2 (de) Megakaryozyten-Differenzierungsfaktor
CN116574172B (zh) 重组人源化i型胶原蛋白及其制备方法
KR920702417A (ko) Tcf-ii의 아미노산 배열을 코드하는 dna를 함유한 플라스미드, 형질전환세포, 및 이것을 사용하여 생리 활성물질을 생산하는 방법
EP0875567B1 (de) Myc-bindende Zinkfinger-Proteine, ihre Herstellung und ihre Verwendung
KR900014428A (ko) 신규폴리펩타이드 및 그 제조방법
KR910006483A (ko) 재조합 아프로티닌 변이체, 균질하게 프로세싱된 아프로티닌 변이체의 미생물을 이용한 유전공학적 제조 방법 및 그의 치료 용도
CA1305439C (en) Production of human lysozyme
KR870010193A (ko) 인체 췌장 엘라스타제 i
DE69310540T2 (de) Verhindern von spaltung von n-terminalen aminosäuren durch endogene aminopeptidasen während die expression von fremden genen in bakterien
ATE253639T1 (de) Verfahren zur herstellung von peptiden
EP0571743A1 (de) Gen-Expression regulierender Faktor
CA2002210C (en) Expression and processing of authentic fgf's in yeast