DE60318240D1 - Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure - Google Patents

Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure

Info

Publication number
DE60318240D1
DE60318240D1 DE60318240T DE60318240T DE60318240D1 DE 60318240 D1 DE60318240 D1 DE 60318240D1 DE 60318240 T DE60318240 T DE 60318240T DE 60318240 T DE60318240 T DE 60318240T DE 60318240 D1 DE60318240 D1 DE 60318240D1
Authority
DE
Germany
Prior art keywords
amino acid
trna synthetase
unnatural amino
tyrosyl
tyrosine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60318240T
Other languages
English (en)
Other versions
DE60318240T2 (de
Inventor
Shigeyuki Yokoyama
Mikako Shirouzu
Ayako Sakamoto
Kensaku Sakamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Riken Wako Saitama Jp
Original Assignee
Japan Science and Technology Agency
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency, RIKEN Institute of Physical and Chemical Research filed Critical Japan Science and Technology Agency
Application granted granted Critical
Publication of DE60318240D1 publication Critical patent/DE60318240D1/de
Publication of DE60318240T2 publication Critical patent/DE60318240T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60318240T 2002-10-31 2003-10-31 Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure Expired - Lifetime DE60318240T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2002318846 2002-10-31
JP2002318846 2002-10-31
PCT/JP2003/014028 WO2004039989A1 (ja) 2002-10-31 2003-10-31 非天然型アミノ酸組み込みタンパク質の発現方法

Publications (2)

Publication Number Publication Date
DE60318240D1 true DE60318240D1 (de) 2008-01-31
DE60318240T2 DE60318240T2 (de) 2008-12-04

Family

ID=32211789

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60318240T Expired - Lifetime DE60318240T2 (de) 2002-10-31 2003-10-31 Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure

Country Status (7)

Country Link
US (2) US7566555B2 (de)
EP (1) EP1557469B1 (de)
JP (1) JP4467435B2 (de)
AT (1) ATE381623T1 (de)
CA (1) CA2503749A1 (de)
DE (1) DE60318240T2 (de)
WO (1) WO2004039989A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003014354A1 (en) * 2001-08-01 2003-02-20 Japan Science And Technology Agency Tyrosyl-trna synthase variants
JP2008035702A (ja) * 2004-11-26 2008-02-21 Institute Of Physical & Chemical Research 蛋白質間相互作用の検出方法
WO2007061136A1 (ja) 2005-11-24 2007-05-31 Riken 非天然型アミノ酸を組み込んだタンパク質の製造方法
JP5196378B2 (ja) * 2006-02-22 2013-05-15 独立行政法人理化学研究所 サプレッサーｔＲＮＡの合成方法、ＤＮＡ構築物及びそれを用いた非天然型アミノ酸組み込みタンパク質の製造
EP2048233B8 (de) * 2006-06-28 2012-05-02 Riken Seprs-mutante und verfahren zur stellengerichteten einführung von phosphoserin in protein unter verwendung davon
US8420792B2 (en) * 2006-09-08 2013-04-16 Ambrx, Inc. Suppressor tRNA transcription in vertebrate cells
CA2662753C (en) * 2006-09-08 2016-02-23 Ambrx, Inc. Hybrid suppressor trna for vertebrate cells
KR20090057072A (ko) * 2006-09-08 2009-06-03 암브룩스, 인코포레이티드 척추동물 세포에 의한 비천연 아미노산의 부위 특이적 도입
JP2010506591A (ja) * 2006-10-18 2010-03-04 ザ スクリップス リサーチ インスティテュート 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み
JP5322195B2 (ja) * 2007-03-30 2013-10-23 独立行政法人理化学研究所 光反応性官能基を有する非天然型アミノ酸組み込みタンパク質の合成方法
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
JP5679147B2 (ja) * 2008-02-07 2015-03-04 独立行政法人理化学研究所 非天然塩基を含む改変ｔＲＮＡ及びその用途
US20090286968A1 (en) 2008-04-25 2009-11-19 Auburn University 2-Quinoxalinol Salen Compounds and Uses Thereof
EP3576764A4 (de) 2017-02-03 2020-12-16 The Medical College of Wisconsin, Inc. Kras-peptid-impfstoffzusammensetzungen und verfahren zur verwendung
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
KR102557569B1 (ko) * 2021-03-04 2023-07-20 전남대학교산학협력단 메타노사에타 콘실리에서 유래한 티로실-tRNA 합성효소 및 이를 이용한 단백질 생산방법

Also Published As

Publication number Publication date
EP1557469B1 (de) 2007-12-19
US7666640B2 (en) 2010-02-23
DE60318240T2 (de) 2008-12-04
JPWO2004039989A1 (ja) 2006-03-02
JP4467435B2 (ja) 2010-05-26
US20060234339A1 (en) 2006-10-19
CA2503749A1 (en) 2004-05-13
US7566555B2 (en) 2009-07-28
EP1557469A1 (de) 2005-07-27
ATE381623T1 (de) 2008-01-15
EP1557469A4 (de) 2005-12-14
WO2004039989A1 (ja) 2004-05-13
US20090286311A1 (en) 2009-11-19

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: RIKEN, WAKO, SAITAMA, JP