ATE381623T1 - Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure - Google Patents

Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure

Info

Publication number
ATE381623T1
ATE381623T1 AT03809875T AT03809875T ATE381623T1 AT E381623 T1 ATE381623 T1 AT E381623T1 AT 03809875 T AT03809875 T AT 03809875T AT 03809875 T AT03809875 T AT 03809875T AT E381623 T1 ATE381623 T1 AT E381623T1
Authority
AT
Austria
Prior art keywords
amino acid
trna synthetase
unnatural amino
tyrosyl
tyrosine
Prior art date
Application number
AT03809875T
Other languages
English (en)
Inventor
Shigeyuki Yokoyama
Mikako Shirouzu
Ayako Sakamoto
Kensaku Sakamoto
Original Assignee
Riken
Japan Science & Tech Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken, Japan Science & Tech Agency filed Critical Riken
Application granted granted Critical
Publication of ATE381623T1 publication Critical patent/ATE381623T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT03809875T 2002-10-31 2003-10-31 Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure ATE381623T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002318846 2002-10-31

Publications (1)

Publication Number Publication Date
ATE381623T1 true ATE381623T1 (de) 2008-01-15

Family

ID=32211789

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03809875T ATE381623T1 (de) 2002-10-31 2003-10-31 Verfahren zur expression von protein mit darin integrierter unnatürlicher aminosäure

Country Status (7)

Country Link
US (2) US7566555B2 (de)
EP (1) EP1557469B1 (de)
JP (1) JP4467435B2 (de)
AT (1) ATE381623T1 (de)
CA (1) CA2503749A1 (de)
DE (1) DE60318240T2 (de)
WO (1) WO2004039989A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4263598B2 (ja) * 2001-08-01 2009-05-13 独立行政法人科学技術振興機構 チロシルｔＲＮＡ合成酵素変異体
JP2008035702A (ja) * 2004-11-26 2008-02-21 Institute Of Physical & Chemical Research 蛋白質間相互作用の検出方法
EP1961817B1 (de) 2005-11-24 2013-03-13 Riken Verfahren zur herstellung eines proteins mit darin integrierter nichtnatürlicher aminosäure
WO2007099854A1 (ja) * 2006-02-22 2007-09-07 Riken サプレッサーｔＲＮＡの合成方法、ＤＮＡ構築物及びそれを用いた非天然型アミノ酸組み込みタンパク質の製造
JP5305440B2 (ja) * 2006-06-28 2013-10-02 独立行政法人理化学研究所 変異体ＳｅｐＲＳ及びこれを用いるタンパク質への部位特異的ホスホセリン導入法
ATE542888T1 (de) * 2006-09-08 2012-02-15 Ambrx Inc Standortspezifische inkorporation nicht natürlicher aminosäuren in wirbeltierzellen
CN101511856B (zh) * 2006-09-08 2016-01-20 Ambrx公司 脊椎动物细胞中抑制因子trna的转录
US9133495B2 (en) * 2006-09-08 2015-09-15 Ambrx, Inc. Hybrid suppressor tRNA for vertebrate cells
CN101535338A (zh) * 2006-10-18 2009-09-16 斯克利普斯研究院 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质
JP5322195B2 (ja) * 2007-03-30 2013-10-23 独立行政法人理化学研究所 光反応性官能基を有する非天然型アミノ酸組み込みタンパク質の合成方法
CN101965397B (zh) 2008-01-03 2016-09-28 巴斯夫酶有限责任公司 转移酶和氧化还原酶、编码它们的核酸以及其制备和应用方法
JP5679147B2 (ja) * 2008-02-07 2015-03-04 独立行政法人理化学研究所 非天然塩基を含む改変ｔＲＮＡ及びその用途
US20090286968A1 (en) 2008-04-25 2009-11-19 Auburn University 2-Quinoxalinol Salen Compounds and Uses Thereof
EP3576764A4 (de) 2017-02-03 2020-12-16 The Medical College of Wisconsin, Inc. Kras-peptid-impfstoffzusammensetzungen und verfahren zur verwendung
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
KR102557569B1 (ko) * 2021-03-04 2023-07-20 전남대학교산학협력단 메타노사에타 콘실리에서 유래한 티로실-tRNA 합성효소 및 이를 이용한 단백질 생산방법

Also Published As

Publication number Publication date
EP1557469B1 (de) 2007-12-19
JPWO2004039989A1 (ja) 2006-03-02
DE60318240T2 (de) 2008-12-04
EP1557469A1 (de) 2005-07-27
DE60318240D1 (de) 2008-01-31
US7666640B2 (en) 2010-02-23
US20090286311A1 (en) 2009-11-19
CA2503749A1 (en) 2004-05-13
US7566555B2 (en) 2009-07-28
JP4467435B2 (ja) 2010-05-26
EP1557469A4 (de) 2005-12-14
US20060234339A1 (en) 2006-10-19
WO2004039989A1 (ja) 2004-05-13

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