DE60335637D1 - Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren - Google Patents
Vektoren auf transposonbasis und verfahren zur integration von nukleinsäurenInfo
- Publication number
- DE60335637D1 DE60335637D1 DE60335637T DE60335637T DE60335637D1 DE 60335637 D1 DE60335637 D1 DE 60335637D1 DE 60335637 T DE60335637 T DE 60335637T DE 60335637 T DE60335637 T DE 60335637T DE 60335637 D1 DE60335637 D1 DE 60335637D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acids
- transposone
- base vectors
- integrating nucleic
- integrating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/75—Vector systems having a special element relevant for transcription from invertebrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39862802P | 2002-07-24 | 2002-07-24 | |
PCT/US2003/023090 WO2004009792A2 (en) | 2002-07-24 | 2003-07-24 | Transposon-based vectors and methods of nucleic acid integration |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60335637D1 true DE60335637D1 (de) | 2011-02-17 |
Family
ID=30771227
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60335637T Expired - Lifetime DE60335637D1 (de) | 2002-07-24 | 2003-07-24 | Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren |
Country Status (9)
Country | Link |
---|---|
US (1) | US20060210977A1 (de) |
EP (1) | EP1546322B1 (de) |
AT (1) | ATE494370T1 (de) |
AU (1) | AU2003268022B2 (de) |
CA (1) | CA2533708C (de) |
DE (1) | DE60335637D1 (de) |
ES (1) | ES2358994T3 (de) |
SI (1) | SI1546322T1 (de) |
WO (1) | WO2004009792A2 (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7527966B2 (en) * | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
US20040172667A1 (en) | 2002-06-26 | 2004-09-02 | Cooper Richard K. | Administration of transposon-based vectors to reproductive organs |
DK1594972T3 (da) * | 2003-02-10 | 2011-04-18 | Max Delbrueck Centrum | Transposon-baseret målretningssystem |
DE602004031284D1 (de) * | 2003-02-10 | 2011-03-17 | Max Delbrueck Centrum | Transposon-system zur gezielten integration |
ATE536419T1 (de) * | 2003-02-10 | 2011-12-15 | Max Delbrueck Centrum | Transposon basiertes targeting- system |
WO2005062881A2 (en) | 2003-12-24 | 2005-07-14 | Transgenrx, Inc. | Gene therapy using transposon-based vectors |
WO2005089123A2 (en) * | 2004-03-04 | 2005-09-29 | Massachusetts Institute Of Technology | Therapeutic anti-cancer dna |
EP1755640A4 (de) * | 2004-04-30 | 2009-10-28 | Poetic Genetics Llc | Hybridrekombinasen für die genom-manipulation |
US7470777B2 (en) | 2004-12-22 | 2008-12-30 | Iowa State University Research Foundation, Inc. | Compositions and methods related to modified retroviral vectors for restricted, site specific integration |
US7919583B2 (en) | 2005-08-08 | 2011-04-05 | Discovery Genomics, Inc. | Integration-site directed vector systems |
WO2008100424A2 (en) * | 2007-02-09 | 2008-08-21 | University Of Hawaii | Animals and cells with genomic target sites for transposase-mediated transgenesis |
WO2008137114A1 (en) * | 2007-05-04 | 2008-11-13 | University Of Hawai'i | Methods and compositions for targeted delivery of gene therapeutic vectors |
US20090042297A1 (en) * | 2007-06-01 | 2009-02-12 | George Jr Alfred L | Piggybac transposon-based vectors and methods of nucleic acid integration |
EP2167519A4 (de) * | 2007-06-16 | 2011-04-13 | Scarab Genomics Llc | Nukleinsäureverpackungssystem |
CN101503670B (zh) * | 2008-02-04 | 2011-12-14 | 复旦大学附属华山医院 | 一种含有整合子的工程菌株 |
WO2010008562A2 (en) * | 2008-07-16 | 2010-01-21 | Recombinetics | Methods and materials for producing transgenic animals |
WO2010036978A2 (en) | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of growth hormone |
WO2010036976A2 (en) | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of antibodies |
WO2010085699A2 (en) * | 2009-01-23 | 2010-07-29 | The Johns Hopkins University | Mammalian piggybac transposon and methods of use |
GB2467167B (en) | 2009-01-26 | 2013-09-11 | Algentech Sas | Gene targeting in plants |
EP2417263B1 (de) | 2009-04-09 | 2015-09-23 | ProteoVec Holding L.L.C. | Herstellung von proteinen unter verwendung von vektoren auf transposonbasis |
JP6087148B2 (ja) * | 2010-12-15 | 2017-03-01 | 大学共同利用機関法人情報・システム研究機構 | タンパク質の生産方法 |
CA2919503C (en) | 2013-07-12 | 2019-06-25 | University Of South Alabama | Minimal piggybac vectors for genome integration |
EP3352798A1 (de) * | 2015-09-22 | 2018-08-01 | Julius-Maximilians-Universität Würzburg | Verfahren zum hochgradigen und stabilen gentransfer in lymphozyten |
CN105154473B (zh) * | 2015-09-30 | 2019-03-01 | 上海细胞治疗研究院 | 一种高效安全的转座子整合系统及其用途 |
CN105481984B (zh) * | 2015-12-03 | 2020-09-22 | 上海细胞治疗研究院 | 一种高效介导外源基因整合的转座酶及其用途 |
AU2019245243A1 (en) | 2018-03-29 | 2020-09-03 | Genentech, Inc | Modulating lactogenic activity in mammalian cells |
US20220090142A1 (en) | 2019-02-13 | 2022-03-24 | Probiogen Ag | Transposase with enhanced insertion site selection properties |
CA3141422A1 (en) * | 2019-06-11 | 2020-12-17 | Avencia Sanchez-mejias Garcia | Targeted gene editing constructs and methods of using the same |
EP3835421A1 (de) * | 2019-12-11 | 2021-06-16 | Consejo Superior de Investigaciones Científicas (CSIC) | Vektoren für gewebespezifische transkriptomik |
CA3169908A1 (en) | 2020-03-26 | 2021-09-30 | Genentech, Inc. | Modified mammalian cells having reduced host cell proteins |
WO2021226141A1 (en) | 2020-05-04 | 2021-11-11 | Saliogen Therapeutics, Inc. | Transposition-based therapies |
KR20230026491A (ko) | 2020-06-24 | 2023-02-24 | 제넨테크, 인크. | 아폽토시스 내성 세포주 |
CN117015600A (zh) | 2020-07-17 | 2023-11-07 | 普罗拜奥根股份公司 | 高活性转座子和转座酶 |
CA3215965A1 (en) | 2021-04-19 | 2022-10-27 | Amy Shen | Modified mammalian cells |
CN117396599A (zh) | 2021-05-21 | 2024-01-12 | 基因泰克公司 | 用于生产目的重组产物的经修饰的细胞 |
WO2023039434A1 (en) * | 2021-09-08 | 2023-03-16 | Metagenomi, Inc. | Systems and methods for transposing cargo nucleotide sequences |
TW202342755A (zh) | 2021-12-22 | 2023-11-01 | 美商建南德克公司 | 多載體重組酶介導的匣式交換 |
WO2024068845A1 (en) * | 2022-09-29 | 2024-04-04 | Vib Vzw | New loxpsym sites for large-scale orthogonal cre-mediated recombination |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6225121B1 (en) * | 1992-09-14 | 2001-05-01 | Institute Of Molecular Biology And Biotechnology/Forth | Eukaryotic transposable element |
US6150511A (en) * | 1995-05-09 | 2000-11-21 | Fox Chase Cancer Center | Chimeric enzyme for promoting targeted integration of foreign DNA into a host genome |
JPH11505128A (ja) * | 1995-05-22 | 1999-05-18 | カイロン コーポレイション | キメラインテグラーゼタンパク質に媒介される真核生物ゲノム中へのベクター構築物の位置特異的組み込み |
EP0871711A1 (de) * | 1995-12-01 | 1998-10-21 | The Regents Of The University Of California | Zusammensetzungen und methoden für die ortsspezifische integration in dns |
EP1071471B1 (de) * | 1998-04-15 | 2008-12-31 | Fred Hutchinson Cancer Research Center | Methoden und vektorenkonstrukte zur erzeugung von transgene nagetieren, welche ein heterologes gen ubiquitär exprimieren |
WO2001030965A2 (en) * | 1999-10-28 | 2001-05-03 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of in vivo gene transfer using a sleeping beauty transposon system |
EP1303608A2 (de) * | 2000-07-21 | 2003-04-23 | Syngenta Participations AG | Code zur erkennung von zinkfingerdomänen und dessen verwendungen |
US20020132350A1 (en) * | 2000-09-14 | 2002-09-19 | Pioneer Hi-Bred International, Inc. | Targeted genetic manipulation using Mu bacteriophage cleaved donor complex |
EP1308516A1 (de) * | 2001-10-24 | 2003-05-07 | ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire | Zielgerichtete Rekombinase-Fusionsproteine und entsprechende Polynukleotide, Vektoren und Kits, und deren Verwendungen zur zielgerichteten DNS-Rekombination |
-
2003
- 2003-07-24 EP EP03748973A patent/EP1546322B1/de not_active Expired - Lifetime
- 2003-07-24 US US10/521,936 patent/US20060210977A1/en not_active Abandoned
- 2003-07-24 DE DE60335637T patent/DE60335637D1/de not_active Expired - Lifetime
- 2003-07-24 SI SI200331963T patent/SI1546322T1/sl unknown
- 2003-07-24 ES ES03748973T patent/ES2358994T3/es not_active Expired - Lifetime
- 2003-07-24 WO PCT/US2003/023090 patent/WO2004009792A2/en not_active Application Discontinuation
- 2003-07-24 AU AU2003268022A patent/AU2003268022B2/en not_active Ceased
- 2003-07-24 AT AT03748973T patent/ATE494370T1/de not_active IP Right Cessation
- 2003-07-24 CA CA2533708A patent/CA2533708C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA2533708C (en) | 2013-05-14 |
ES2358994T3 (es) | 2011-05-17 |
EP1546322B1 (de) | 2011-01-05 |
ATE494370T1 (de) | 2011-01-15 |
EP1546322A2 (de) | 2005-06-29 |
AU2003268022B2 (en) | 2009-02-05 |
EP1546322A4 (de) | 2006-07-19 |
SI1546322T1 (sl) | 2011-05-31 |
US20060210977A1 (en) | 2006-09-21 |
WO2004009792A2 (en) | 2004-01-29 |
AU2003268022A1 (en) | 2004-02-09 |
WO2004009792A3 (en) | 2004-05-13 |
CA2533708A1 (en) | 2004-01-29 |
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