DE60237792D1 - Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen - Google Patents
Verfahren zur Verwendung von O6-Alkylguanin-DNS AlkyltransferasenInfo
- Publication number
- DE60237792D1 DE60237792D1 DE60237792T DE60237792T DE60237792D1 DE 60237792 D1 DE60237792 D1 DE 60237792D1 DE 60237792 T DE60237792 T DE 60237792T DE 60237792 T DE60237792 T DE 60237792T DE 60237792 D1 DE60237792 D1 DE 60237792D1
- Authority
- DE
- Germany
- Prior art keywords
- cell
- agt
- protein
- alkylguanine
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000002226 Alkyl and Aryl Transferases Human genes 0.000 title abstract 2
- 108010014722 Alkyl and Aryl Transferases Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 4
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 210000001723 extracellular space Anatomy 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28276601P | 2001-04-10 | 2001-04-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60237792D1 true DE60237792D1 (de) | 2010-11-04 |
Family
ID=23083030
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60230689T Expired - Lifetime DE60230689D1 (de) | 2001-04-10 | 2002-04-05 | Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen |
| DE60212642T Expired - Lifetime DE60212642T2 (de) | 2001-04-10 | 2002-04-05 | Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen |
| DE60237792T Expired - Lifetime DE60237792D1 (de) | 2001-04-10 | 2002-04-05 | Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60230689T Expired - Lifetime DE60230689D1 (de) | 2001-04-10 | 2002-04-05 | Verfahren zur Verwendung von O6-Alkylguanin-DNS Alkyltransferasen |
| DE60212642T Expired - Lifetime DE60212642T2 (de) | 2001-04-10 | 2002-04-05 | Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7939284B2 (enExample) |
| EP (4) | EP2211177B1 (enExample) |
| JP (2) | JP4195815B2 (enExample) |
| KR (1) | KR20040039194A (enExample) |
| CN (2) | CN1975422B (enExample) |
| AT (3) | ATE331220T1 (enExample) |
| AU (1) | AU2002251257B2 (enExample) |
| CA (1) | CA2443570A1 (enExample) |
| DE (3) | DE60230689D1 (enExample) |
| DK (1) | DK1410023T3 (enExample) |
| IL (1) | IL157880A0 (enExample) |
| NZ (3) | NZ540410A (enExample) |
| WO (1) | WO2002083937A2 (enExample) |
| ZA (1) | ZA200307442B (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1546371A1 (en) * | 2002-10-03 | 2005-06-29 | Ecole Polytechnique Federale De Lausanne (Epfl) | Substrates for o6-alkylguanine-dna alkyltransferase |
| WO2004031404A1 (en) | 2002-10-03 | 2004-04-15 | Ecole Polytechnique Federale De Lausanne (Epfl) | Protein labelling with o6-alkylguanine-dna alkyltransferase |
| AU2004211584B2 (en) | 2003-01-31 | 2009-11-12 | Promega Corporation | Covalent tethering of functional groups to proteins |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| US7666612B2 (en) * | 2003-05-23 | 2010-02-23 | Epfl-Ecole Polytechnique Federale De Lausanne | Methods for protein labeling based on acyl carrier protein |
| JP2007526282A (ja) * | 2004-03-02 | 2007-09-13 | ウペエフエル・エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ | Ｏ６−アルキルグアニンｄｎａアルキルトランスフェラーゼ特異的基質 |
| WO2005085431A2 (en) * | 2004-03-02 | 2005-09-15 | Epfl Ecole Polytechnique Federale De Lausanne | Mutants of o6-alkylguanine-dna alkyltransferase |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| US7825096B2 (en) | 2004-09-08 | 2010-11-02 | The United States Of America As Represented By The Department Of Health And Human Services | O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs |
| US7273714B2 (en) * | 2005-03-11 | 2007-09-25 | Vion Pharmaceuticals, Inc. | Alkylguanyltransferase assays |
| JP2008539198A (ja) * | 2005-04-27 | 2008-11-13 | コヴァリス・バイオサイエンシス・アーゲー | Ｏ６−アルキルグアニン−ｄｎａアルキルトランスフェラーゼと反応するピリミジン |
| FR2890174B1 (fr) | 2005-08-30 | 2009-04-24 | Cis Bio Internat Sa | Procede pour la mise en evidence d'un processus biologique par mesure d'un fret |
| FR2890446B1 (fr) | 2005-09-05 | 2008-04-18 | Cis Bio Internat Sa | Methode de detection d'interaction intracellulaire entre bio-molecules |
| EP2044219B1 (en) | 2006-06-30 | 2013-05-22 | DiscoveRx Corporation | Detectable nucleic acid tag |
| WO2008054821A2 (en) | 2006-10-30 | 2008-05-08 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
| JP5513122B2 (ja) * | 2006-12-05 | 2014-06-04 | レーザーゲン インコーポレイテッド | 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法 |
| US7897737B2 (en) | 2006-12-05 | 2011-03-01 | Lasergen, Inc. | 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing |
| EP1978093A1 (en) * | 2007-04-04 | 2008-10-08 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Method of crosslinking two objects of interest |
| GB0711560D0 (en) * | 2007-06-14 | 2007-07-25 | Innova Biosciences Ltd | Improvements in or relating to production of conjugates |
| US20100209933A1 (en) * | 2007-10-29 | 2010-08-19 | Mcreynolds Larry A | Methods and Compositions for Detection and Enrichment of Target Small RNAs |
| EP2093564A1 (en) | 2008-02-25 | 2009-08-26 | Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus | Method for distinguishing secretory granules of different ages |
| KR101677772B1 (ko) | 2008-06-11 | 2016-11-18 | 레이저젠, 인코퍼레이티드 | 뉴클레오타이드 및 뉴클레오사이드 그리고 시퀀싱에서의 이들의 이용 방법 |
| FR2936245B1 (fr) * | 2008-09-23 | 2012-07-06 | Cis Bio Int | Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants. |
| DE112011100297B4 (de) | 2010-01-20 | 2017-08-24 | New England Biolabs, Inc. | Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA |
| US20130183696A1 (en) | 2010-09-15 | 2013-07-18 | Constance Neely Wilson | Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay |
| CA2848049C (en) | 2011-09-13 | 2021-01-26 | Lasergen, Inc. | 5-methoxy, 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing |
| EP2788478B1 (en) | 2011-12-09 | 2017-08-23 | Institut Pasteur | Multiplex immuno screening assay |
| US9267117B2 (en) | 2012-03-15 | 2016-02-23 | New England Biolabs, Inc. | Mapping cytosine modifications |
| US9567632B2 (en) | 2013-03-19 | 2017-02-14 | New England Biolabs, Inc. | Enrichment of target sequences |
| JP5781589B2 (ja) * | 2013-12-20 | 2015-09-24 | レーザーゲン インコーポレイテッド | 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法 |
| WO2016003795A1 (en) | 2014-07-02 | 2016-01-07 | New England Biolabs, Inc. | Universal n-glycan binding reagent |
| JP2017537657A (ja) | 2014-12-11 | 2017-12-21 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | 標的配列の濃縮 |
| CN104844710B (zh) * | 2015-04-13 | 2018-02-02 | 西北大学 | 定向固定化pega复合型树酯的制备方法及应用 |
| CN109661407A (zh) | 2016-06-06 | 2019-04-19 | 西北大学 | 融合蛋白构建体 |
| CN113444094B (zh) | 2016-07-20 | 2023-12-22 | 纳莹(上海)生物科技有限公司 | 一种荧光探针及其制备方法和用途 |
| US10934533B1 (en) | 2018-10-10 | 2021-03-02 | New England Biolabs, Inc. | Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA |
| CN111253417B (zh) * | 2019-09-04 | 2022-03-25 | 大连民族大学 | 一种嘌呤衍生物的合成及应用 |
| WO2021113290A1 (en) | 2019-12-03 | 2021-06-10 | Alamar Biosciences, Inc. | Nucleic acid linked immune-sandwich assay (nulisa) |
| EP4103701A1 (en) | 2020-02-13 | 2022-12-21 | New England Biolabs, Inc. | Variant family d dna polymerases |
| WO2021163559A1 (en) | 2020-02-13 | 2021-08-19 | New England Biolabs, Inc. | Variant family d dna polymerases |
| WO2021183921A1 (en) | 2020-03-12 | 2021-09-16 | New England Biolabs, Inc. | A rapid diagnostic test for lamp |
| CN112010983A (zh) * | 2020-08-11 | 2020-12-01 | 西北大学 | 止咳平喘中药活性成分靶向筛选方法及试剂盒 |
| EP4110945A2 (en) | 2020-08-21 | 2023-01-04 | New England Biolabs, Inc. | A rapid diagnostic test for lamp |
| WO2022250676A1 (en) | 2021-05-27 | 2022-12-01 | New England Biolabs, Inc. | Dnase i variants, compositions, methods, and kits |
| US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
| AU2022396419A1 (en) | 2021-11-24 | 2024-05-23 | New England Biolabs, Inc. | Double-stranded dna deaminases |
| WO2023197015A1 (en) | 2022-04-08 | 2023-10-12 | New England Biolabs, Inc. | Compositions and analysis of dephosphorylated oligoribonucleotides |
| EP4658673A1 (en) | 2023-02-01 | 2025-12-10 | New England Biolabs, Inc. | Duplex-specific dnases |
| WO2025076551A1 (en) | 2023-10-05 | 2025-04-10 | New England Biolabs, Inc. | Methyl cytosine-selective deaminases and uses thereof |
| WO2025129193A1 (en) | 2023-12-15 | 2025-06-19 | New England Biolabs, Inc. | Glycosyltransferases and related methods |
| WO2025137453A1 (en) | 2023-12-20 | 2025-06-26 | New England Biolabs, Inc. | Compositions, methods, kits, and instruments for analyzing rna structure |
| GB202400129D0 (en) | 2024-01-04 | 2024-02-21 | Cambridge Entpr Ltd | Nucleic acid barcodes |
| WO2025224010A1 (en) | 2024-04-24 | 2025-10-30 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. | Improved o6-alkylguanine-dna alkyltransferase substrates |
| WO2025227034A1 (en) | 2024-04-26 | 2025-10-30 | New England Biolabs, Inc. | Salt active nuclease compositions and methods |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9224888D0 (en) * | 1992-11-27 | 1993-01-13 | Univ Singapore | Monoclonal antibody |
| JP4054057B2 (ja) * | 1993-06-08 | 2008-02-27 | キャンサー・リサーチ・テクノロジー・リミテッド | Ｏ▲上６▼−置換グアニン誘導体、それらの製造方法および腫瘍細胞の処置におけるそれらの使用 |
| DE19903895A1 (de) * | 1999-02-01 | 2000-08-03 | Kai Johnsson | Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form |
-
2002
- 2002-04-05 NZ NZ540410A patent/NZ540410A/en unknown
- 2002-04-05 CN CN2006101603317A patent/CN1975422B/zh not_active Expired - Lifetime
- 2002-04-05 EP EP10075132.0A patent/EP2211177B1/en not_active Expired - Lifetime
- 2002-04-05 CN CNB028081137A patent/CN1295510C/zh not_active Expired - Lifetime
- 2002-04-05 KR KR10-2003-7012899A patent/KR20040039194A/ko not_active Abandoned
- 2002-04-05 AT AT02720187T patent/ATE331220T1/de not_active IP Right Cessation
- 2002-04-05 DK DK02720187T patent/DK1410023T3/da active
- 2002-04-05 WO PCT/GB2002/001636 patent/WO2002083937A2/en not_active Ceased
- 2002-04-05 EP EP08168182A patent/EP2037271B1/en not_active Expired - Lifetime
- 2002-04-05 DE DE60230689T patent/DE60230689D1/de not_active Expired - Lifetime
- 2002-04-05 EP EP06111733A patent/EP1696234B1/en not_active Expired - Lifetime
- 2002-04-05 DE DE60212642T patent/DE60212642T2/de not_active Expired - Lifetime
- 2002-04-05 EP EP02720187A patent/EP1410023B1/en not_active Expired - Lifetime
- 2002-04-05 JP JP2002582274A patent/JP4195815B2/ja not_active Expired - Lifetime
- 2002-04-05 US US10/474,796 patent/US7939284B2/en not_active Expired - Lifetime
- 2002-04-05 DE DE60237792T patent/DE60237792D1/de not_active Expired - Lifetime
- 2002-04-05 NZ NZ537939A patent/NZ537939A/en unknown
- 2002-04-05 IL IL15788002A patent/IL157880A0/xx not_active IP Right Cessation
- 2002-04-05 NZ NZ528594A patent/NZ528594A/en unknown
- 2002-04-05 AT AT06111733T patent/ATE419378T1/de not_active IP Right Cessation
- 2002-04-05 AT AT08168182T patent/ATE482395T1/de not_active IP Right Cessation
- 2002-04-05 AU AU2002251257A patent/AU2002251257B2/en not_active Ceased
- 2002-04-05 CA CA002443570A patent/CA2443570A1/en not_active Abandoned
-
2003
- 2003-09-25 ZA ZA200307442A patent/ZA200307442B/xx unknown
-
2007
- 2007-12-20 JP JP2007328757A patent/JP4226053B2/ja not_active Expired - Fee Related
-
2011
- 2011-01-24 US US13/012,234 patent/US8367361B2/en not_active Expired - Fee Related
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