JP4195815B2 - O6−アルキルグアニン−dnaアルキルトランスフェラーゼ(agt)の使用方法 - Google Patents

O6−アルキルグアニン−dnaアルキルトランスフェラーゼ(agt)の使用方法 Download PDF

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Publication number
JP4195815B2
JP4195815B2 JP2002582274A JP2002582274A JP4195815B2 JP 4195815 B2 JP4195815 B2 JP 4195815B2 JP 2002582274 A JP2002582274 A JP 2002582274A JP 2002582274 A JP2002582274 A JP 2002582274A JP 4195815 B2 JP4195815 B2 JP 4195815B2
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fusion protein
agt
protein
label
substrate
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Expired - Lifetime
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Japanese (ja)
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JP2004532028A5 (enExample
JP2004532028A (ja
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ジョンソン,カイ
ジャンドライツィヒ,スザンヌ
ケップラー,アントイェ
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Ecole Polytechnique Federale de Lausanne EPFL
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Ecole Polytechnique Federale de Lausanne EPFL
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2002582274A 2001-04-10 2002-04-05 O6−アルキルグアニン−dnaアルキルトランスフェラーゼ(agt)の使用方法 Expired - Lifetime JP4195815B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

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JP2007328757A Division JP4226053B2 (ja) 2001-04-10 2007-12-20 O6−アルキルグアニン−dnaアルキルトランスフェラーゼ(agt)の使用方法

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JP2004532028A JP2004532028A (ja) 2004-10-21
JP2004532028A5 JP2004532028A5 (enExample) 2008-10-02
JP4195815B2 true JP4195815B2 (ja) 2008-12-17

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JP2007328757A Expired - Fee Related JP4226053B2 (ja) 2001-04-10 2007-12-20 O6−アルキルグアニン−dnaアルキルトランスフェラーゼ(agt)の使用方法

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US (2) US7939284B2 (enExample)
EP (4) EP2211177B1 (enExample)
JP (2) JP4195815B2 (enExample)
KR (1) KR20040039194A (enExample)
CN (2) CN1975422B (enExample)
AT (3) ATE331220T1 (enExample)
AU (1) AU2002251257B2 (enExample)
CA (1) CA2443570A1 (enExample)
DE (3) DE60230689D1 (enExample)
DK (1) DK1410023T3 (enExample)
IL (1) IL157880A0 (enExample)
NZ (3) NZ540410A (enExample)
WO (1) WO2002083937A2 (enExample)
ZA (1) ZA200307442B (enExample)

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JP2006501286A (ja) * 2002-10-03 2006-01-12 エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ(エーペーエフエル) O6−アルキルグアニン−dnaアルキルトランスフェラーゼ

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US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
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JP2007526282A (ja) * 2004-03-02 2007-09-13 ウペエフエル・エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ O6−アルキルグアニンdnaアルキルトランスフェラーゼ特異的基質
WO2005085431A2 (en) * 2004-03-02 2005-09-15 Epfl Ecole Polytechnique Federale De Lausanne Mutants of o6-alkylguanine-dna alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
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US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
US20100209933A1 (en) * 2007-10-29 2010-08-19 Mcreynolds Larry A Methods and Compositions for Detection and Enrichment of Target Small RNAs
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
KR101677772B1 (ko) 2008-06-11 2016-11-18 레이저젠, 인코퍼레이티드 뉴클레오타이드 및 뉴클레오사이드 그리고 시퀀싱에서의 이들의 이용 방법
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
US20130183696A1 (en) 2010-09-15 2013-07-18 Constance Neely Wilson Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
CA2848049C (en) 2011-09-13 2021-01-26 Lasergen, Inc. 5-methoxy, 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
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JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
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CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
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CN113444094B (zh) 2016-07-20 2023-12-22 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
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CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
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JP2006501286A (ja) * 2002-10-03 2006-01-12 エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ(エーペーエフエル) O6−アルキルグアニン−dnaアルキルトランスフェラーゼ

Also Published As

Publication number Publication date
EP2037271B1 (en) 2010-09-22
CN1975422A (zh) 2007-06-06
US20110201514A1 (en) 2011-08-18
CN1527941A (zh) 2004-09-08
WO2002083937A3 (en) 2004-02-26
HK1068406A1 (en) 2005-04-29
EP2037271A1 (en) 2009-03-18
EP1410023B1 (en) 2006-06-21
DE60237792D1 (de) 2010-11-04
EP1696234B1 (en) 2008-12-31
DE60212642D1 (de) 2006-08-03
AU2002251257B2 (en) 2007-08-30
KR20040039194A (ko) 2004-05-10
DE60230689D1 (de) 2009-02-12
CA2443570A1 (en) 2002-10-24
ZA200307442B (en) 2004-06-30
US20040115130A1 (en) 2004-06-17
EP2211177A1 (en) 2010-07-28
US8367361B2 (en) 2013-02-05
NZ540410A (en) 2006-06-30
JP2008154587A (ja) 2008-07-10
DK1410023T3 (da) 2006-10-09
EP1410023A2 (en) 2004-04-21
WO2002083937A2 (en) 2002-10-24
ATE419378T1 (de) 2009-01-15
CN1295510C (zh) 2007-01-17
EP1696234A1 (en) 2006-08-30
EP2211177B1 (en) 2014-07-16
JP2004532028A (ja) 2004-10-21
NZ537939A (en) 2005-08-26
ATE482395T1 (de) 2010-10-15
US7939284B2 (en) 2011-05-10
IL157880A0 (en) 2004-03-28
DE60212642T2 (de) 2007-06-14
ATE331220T1 (de) 2006-07-15
CN1975422B (zh) 2012-09-05
JP4226053B2 (ja) 2009-02-18
NZ528594A (en) 2005-07-29

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