CA2443570A1 - Methods using o6-alkylguanine-dna alkyltransferases - Google Patents

Methods using o6-alkylguanine-dna alkyltransferases Download PDF

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Publication number
CA2443570A1
CA2443570A1 CA002443570A CA2443570A CA2443570A1 CA 2443570 A1 CA2443570 A1 CA 2443570A1 CA 002443570 A CA002443570 A CA 002443570A CA 2443570 A CA2443570 A CA 2443570A CA 2443570 A1 CA2443570 A1 CA 2443570A1
Authority
CA
Canada
Prior art keywords
fusion protein
protein
label
agt
fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002443570A
Other languages
English (en)
French (fr)
Inventor
Kai Johnsson
Susanne Gendreizig
Antje Keppler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecole Polytechnique Federale de Lausanne EPFL
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2443570A1 publication Critical patent/CA2443570A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA002443570A 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases Abandoned CA2443570A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
US60/282,766 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Publications (1)

Publication Number Publication Date
CA2443570A1 true CA2443570A1 (en) 2002-10-24

Family

ID=23083030

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002443570A Abandoned CA2443570A1 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Country Status (14)

Country Link
US (2) US7939284B2 (enExample)
EP (4) EP2211177B1 (enExample)
JP (2) JP4195815B2 (enExample)
KR (1) KR20040039194A (enExample)
CN (2) CN1975422B (enExample)
AT (3) ATE331220T1 (enExample)
AU (1) AU2002251257B2 (enExample)
CA (1) CA2443570A1 (enExample)
DE (3) DE60237792D1 (enExample)
DK (1) DK1410023T3 (enExample)
IL (1) IL157880A0 (enExample)
NZ (3) NZ540410A (enExample)
WO (1) WO2002083937A2 (enExample)
ZA (1) ZA200307442B (enExample)

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US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
AU2004242249A1 (en) * 2003-05-23 2004-12-02 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
WO2005085431A2 (en) * 2004-03-02 2005-09-15 Epfl Ecole Polytechnique Federale De Lausanne Mutants of o6-alkylguanine-dna alkyltransferase
WO2005085470A1 (en) * 2004-03-02 2005-09-15 Epfl Ecole Polytechnique Federale De Lausanne Specific substrates for o6- alkylguanine-dna alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
US7273714B2 (en) * 2005-03-11 2007-09-25 Vion Pharmaceuticals, Inc. Alkylguanyltransferase assays
WO2006114409A1 (en) * 2005-04-27 2006-11-02 Covalys Biosciences Ag Pyrimidines reacting with o6-alkylguanine-dna alkyltransferase
FR2890174B1 (fr) 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
EP2044219B1 (en) 2006-06-30 2013-05-22 DiscoveRx Corporation Detectable nucleic acid tag
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
CN102443030B (zh) * 2006-12-05 2015-12-16 激光基因公司 光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
WO2009058814A1 (en) * 2007-10-29 2009-05-07 New England Biolabs, Inc. Methods and compositions for detection and enrichment of target small rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
US8148503B2 (en) 2008-06-11 2012-04-03 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in DNA sequencing
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
WO2012037361A2 (en) 2010-09-15 2012-03-22 Endacea, Inc. Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
CN104024269B (zh) 2011-09-13 2017-05-24 激光基因公司 5‑甲氧基,3’‑oh未阻断,可快速光切割的终止核苷酸以及用于核酸测序的方法
JP6215223B2 (ja) 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ
US9200260B2 (en) 2012-03-15 2015-12-01 New England Biolabs, Inc. Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
US10080787B2 (en) 2014-07-02 2018-09-25 New England Biolabs, Inc. Universal N-glycan binding reagent
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
WO2017214151A1 (en) 2016-06-06 2017-12-14 Northwestern University Fusion protein construct
CN113444046B (zh) 2016-07-20 2023-10-27 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
EP4069731A4 (en) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
WO2021163559A1 (en) 2020-02-13 2021-08-19 New England Biolabs, Inc. Variant family d dna polymerases
EP4103701A1 (en) 2020-02-13 2022-12-21 New England Biolabs, Inc. Variant family d dna polymerases
EP4118233A1 (en) 2020-03-12 2023-01-18 New England Biolabs, Inc. A rapid diagnostic test for lamp
CN112010983A (zh) * 2020-08-11 2020-12-01 西北大学 止咳平喘中药活性成分靶向筛选方法及试剂盒
EP4110945A2 (en) 2020-08-21 2023-01-04 New England Biolabs, Inc. A rapid diagnostic test for lamp
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
EP4347804A1 (en) 2021-05-27 2024-04-10 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
US20230257730A1 (en) 2021-11-24 2023-08-17 New England Biolabs, Inc. Double-Stranded DNA Deaminases
WO2023197015A1 (en) 2022-04-08 2023-10-12 New England Biolabs, Inc. Compositions and analysis of dephosphorylated oligoribonucleotides
US20240263158A1 (en) 2023-02-01 2024-08-08 New England Biolabs, Inc. Duplex-specific dnases
US20250115953A1 (en) 2023-10-05 2025-04-10 New England Biolabs, Inc. Methylcytosine-Selective Deaminases and Uses Thereof
WO2025129193A1 (en) 2023-12-15 2025-06-19 New England Biolabs, Inc. Glycosyltransferases and related methods
WO2025137453A1 (en) 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025224010A1 (en) 2024-04-24 2025-10-30 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Improved o6-alkylguanine-dna alkyltransferase substrates
WO2025227034A1 (en) 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods

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GB9224888D0 (en) * 1992-11-27 1993-01-13 Univ Singapore Monoclonal antibody
ATE250060T1 (de) * 1993-06-08 2003-10-15 Cancer Rec Tech Ltd O6-substituierte guaninederivate, verfahren zu ihre herstellung und ihre anwendung für behandlung von tumorzellen
DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

Also Published As

Publication number Publication date
NZ540410A (en) 2006-06-30
DK1410023T3 (da) 2006-10-09
CN1527941A (zh) 2004-09-08
EP1410023A2 (en) 2004-04-21
EP2211177A1 (en) 2010-07-28
DE60212642T2 (de) 2007-06-14
US7939284B2 (en) 2011-05-10
ATE482395T1 (de) 2010-10-15
JP4226053B2 (ja) 2009-02-18
WO2002083937A2 (en) 2002-10-24
CN1975422B (zh) 2012-09-05
JP2004532028A (ja) 2004-10-21
DE60230689D1 (de) 2009-02-12
HK1068406A1 (en) 2005-04-29
JP4195815B2 (ja) 2008-12-17
EP1696234A1 (en) 2006-08-30
AU2002251257B2 (en) 2007-08-30
US20040115130A1 (en) 2004-06-17
ATE331220T1 (de) 2006-07-15
NZ537939A (en) 2005-08-26
KR20040039194A (ko) 2004-05-10
ATE419378T1 (de) 2009-01-15
EP1410023B1 (en) 2006-06-21
EP2211177B1 (en) 2014-07-16
CN1295510C (zh) 2007-01-17
EP2037271A1 (en) 2009-03-18
IL157880A0 (en) 2004-03-28
US20110201514A1 (en) 2011-08-18
NZ528594A (en) 2005-07-29
WO2002083937A3 (en) 2004-02-26
JP2008154587A (ja) 2008-07-10
US8367361B2 (en) 2013-02-05
CN1975422A (zh) 2007-06-06
DE60237792D1 (de) 2010-11-04
ZA200307442B (en) 2004-06-30
DE60212642D1 (de) 2006-08-03
EP1696234B1 (en) 2008-12-31
EP2037271B1 (en) 2010-09-22

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued