NZ540410A - Methods using O6-alkylguanine-DNA alkyltransferases - Google Patents
Methods using O6-alkylguanine-DNA alkyltransferasesInfo
- Publication number
- NZ540410A NZ540410A NZ540410A NZ54041002A NZ540410A NZ 540410 A NZ540410 A NZ 540410A NZ 540410 A NZ540410 A NZ 540410A NZ 54041002 A NZ54041002 A NZ 54041002A NZ 540410 A NZ540410 A NZ 540410A
- Authority
- NZ
- New Zealand
- Prior art keywords
- fusion protein
- label
- agt
- protein
- substrate
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 86
- 102000002226 Alkyl and Aryl Transferases Human genes 0.000 title claims abstract description 30
- 108010014722 Alkyl and Aryl Transferases Proteins 0.000 title claims abstract description 30
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 138
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 138
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 18
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- XWNJMSJGJFSGRY-UHFFFAOYSA-N 2-(benzylamino)-3,7-dihydropurin-6-one Chemical compound N1C=2N=CNC=2C(=O)N=C1NCC1=CC=CC=C1 XWNJMSJGJFSGRY-UHFFFAOYSA-N 0.000 claims description 8
- 238000007792 addition Methods 0.000 claims description 8
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- OALHHIHQOFIMEF-UHFFFAOYSA-N 3',6'-dihydroxy-2',4',5',7'-tetraiodo-3h-spiro[2-benzofuran-1,9'-xanthene]-3-one Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 OALHHIHQOFIMEF-UHFFFAOYSA-N 0.000 description 2
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- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 2
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- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- KRWMERLEINMZFT-UHFFFAOYSA-N O6-benzylguanine Chemical class C=12NC=NC2=NC(N)=NC=1OCC1=CC=CC=C1 KRWMERLEINMZFT-UHFFFAOYSA-N 0.000 description 1
- 241000907663 Siproeta stelenes Species 0.000 description 1
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- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
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- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical class O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
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- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
- CPTBDICYNRMXFX-UHFFFAOYSA-N procarbazine Chemical compound CNNCC1=CC=C(C(=O)NC(C)C)C=C1 CPTBDICYNRMXFX-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28276601P | 2001-04-10 | 2001-04-10 | |
| NZ537939A NZ537939A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGT |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ540410A true NZ540410A (en) | 2006-06-30 |
Family
ID=23083030
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ540410A NZ540410A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
| NZ537939A NZ537939A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGT |
| NZ528594A NZ528594A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ537939A NZ537939A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases for labelling a protein of interest which is fused to the AGT |
| NZ528594A NZ528594A (en) | 2001-04-10 | 2002-04-05 | Methods using O6-alkylguanine-DNA alkyltransferases |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7939284B2 (enExample) |
| EP (4) | EP2211177B1 (enExample) |
| JP (2) | JP4195815B2 (enExample) |
| KR (1) | KR20040039194A (enExample) |
| CN (2) | CN1975422B (enExample) |
| AT (3) | ATE331220T1 (enExample) |
| AU (1) | AU2002251257B2 (enExample) |
| CA (1) | CA2443570A1 (enExample) |
| DE (3) | DE60237792D1 (enExample) |
| DK (1) | DK1410023T3 (enExample) |
| IL (1) | IL157880A0 (enExample) |
| NZ (3) | NZ540410A (enExample) |
| WO (1) | WO2002083937A2 (enExample) |
| ZA (1) | ZA200307442B (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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