AU2002251257B2 - Methods of Using O6-alkylguanine-DNA Alkyltransferases - Google Patents

Methods of Using O6-alkylguanine-DNA Alkyltransferases Download PDF

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Publication number
AU2002251257B2
AU2002251257B2 AU2002251257A AU2002251257A AU2002251257B2 AU 2002251257 B2 AU2002251257 B2 AU 2002251257B2 AU 2002251257 A AU2002251257 A AU 2002251257A AU 2002251257 A AU2002251257 A AU 2002251257A AU 2002251257 B2 AU2002251257 B2 AU 2002251257B2
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AU
Australia
Prior art keywords
agt
fusion protein
label
protein
alkylguanine
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Ceased
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AU2002251257A
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AU2002251257A1 (en
Inventor
Susanne Gendreizig
Kai Johnsson
Antje Keppler
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Ecole Polytechnique Federale de Lausanne EPFL
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Ecole Polytechnique Federale de Lausanne EPFL
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Publication of AU2002251257B2 publication Critical patent/AU2002251257B2/en
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Ceased legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2002251257A 2001-04-10 2002-04-05 Methods of Using O6-alkylguanine-DNA Alkyltransferases Ceased AU2002251257B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
US60/282,766 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Publications (2)

Publication Number Publication Date
AU2002251257A1 AU2002251257A1 (en) 2003-04-17
AU2002251257B2 true AU2002251257B2 (en) 2007-08-30

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Family Applications (1)

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AU2002251257A Ceased AU2002251257B2 (en) 2001-04-10 2002-04-05 Methods of Using O6-alkylguanine-DNA Alkyltransferases

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Country Link
US (2) US7939284B2 (enExample)
EP (4) EP2037271B1 (enExample)
JP (2) JP4195815B2 (enExample)
KR (1) KR20040039194A (enExample)
CN (2) CN1975422B (enExample)
AT (3) ATE419378T1 (enExample)
AU (1) AU2002251257B2 (enExample)
CA (1) CA2443570A1 (enExample)
DE (3) DE60230689D1 (enExample)
DK (1) DK1410023T3 (enExample)
IL (1) IL157880A0 (enExample)
NZ (3) NZ537939A (enExample)
WO (1) WO2002083937A2 (enExample)
ZA (1) ZA200307442B (enExample)

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US7888090B2 (en) 2004-03-02 2011-02-15 Ecole Polytechnique Federale De Lausanne Mutants of O6-alkylguanine-DNA alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
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US8178314B2 (en) 2005-04-27 2012-05-15 Covalys Biosciences Ag Pyrimidines reacting with O6-alkylguanine-DNA alkyltransferase fusion protein and method for detecting protein
FR2890174B1 (fr) 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
EP2405272B1 (en) 2006-06-30 2015-01-14 DiscoveRx Corporation Detectable nucleic acid tag
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
JP5513122B2 (ja) * 2006-12-05 2014-06-04 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
ATE522810T1 (de) * 2007-10-29 2011-09-15 New England Biolabs Inc Verfahren und zusammensetzungen zum nachweis und zur anreicherung kleiner ziel-rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
BRPI0915173B8 (pt) 2008-06-11 2021-05-25 Agilent Technologies Inc compostos de nucleotídeos e nucleosídeos e método de sequenciamento de um ácido nucleico-alvo
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
WO2011091146A1 (en) 2010-01-20 2011-07-28 New England Biolabs, Inc. Compositions, methods and related uses for cleaving modified dna
JP6043288B2 (ja) 2010-09-15 2016-12-14 エンダセア, インコーポレイテッド 時間分解蛍光ベースのアッセイによるリポ多糖の測定のための使用方法およびキット
MX342195B (es) 2011-09-13 2016-09-20 Lasergen Inc Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.
JP6215223B2 (ja) * 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ
US9200260B2 (en) 2012-03-15 2015-12-01 New England Biolabs, Inc. Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
US9567632B2 (en) 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにdna配列決定におけるそれらの使用法
US10080787B2 (en) 2014-07-02 2018-09-25 New England Biolabs, Inc. Universal N-glycan binding reagent
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
KR20190015461A (ko) 2016-06-06 2019-02-13 노쓰웨스턴유니버시티 융합 단백질 작제물
CN113444094B (zh) 2016-07-20 2023-12-22 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
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Citations (1)

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DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

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Patent Citations (1)

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Also Published As

Publication number Publication date
KR20040039194A (ko) 2004-05-10
US20110201514A1 (en) 2011-08-18
ZA200307442B (en) 2004-06-30
EP2211177B1 (en) 2014-07-16
CA2443570A1 (en) 2002-10-24
NZ540410A (en) 2006-06-30
EP2211177A1 (en) 2010-07-28
DE60237792D1 (de) 2010-11-04
NZ537939A (en) 2005-08-26
NZ528594A (en) 2005-07-29
EP2037271A1 (en) 2009-03-18
CN1527941A (zh) 2004-09-08
CN1975422B (zh) 2012-09-05
EP2037271B1 (en) 2010-09-22
ATE419378T1 (de) 2009-01-15
JP4195815B2 (ja) 2008-12-17
DE60230689D1 (de) 2009-02-12
EP1696234B1 (en) 2008-12-31
CN1975422A (zh) 2007-06-06
EP1410023A2 (en) 2004-04-21
JP2004532028A (ja) 2004-10-21
US8367361B2 (en) 2013-02-05
DE60212642T2 (de) 2007-06-14
JP2008154587A (ja) 2008-07-10
ATE331220T1 (de) 2006-07-15
US20040115130A1 (en) 2004-06-17
DE60212642D1 (de) 2006-08-03
IL157880A0 (en) 2004-03-28
WO2002083937A2 (en) 2002-10-24
JP4226053B2 (ja) 2009-02-18
DK1410023T3 (da) 2006-10-09
CN1295510C (zh) 2007-01-17
ATE482395T1 (de) 2010-10-15
EP1696234A1 (en) 2006-08-30
WO2002083937A3 (en) 2004-02-26
HK1068406A1 (en) 2005-04-29
EP1410023B1 (en) 2006-06-21
US7939284B2 (en) 2011-05-10

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: DELETE THE INVENTOR SIMON JOHN KIDDLE

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired