AU2002251257B2 - Methods of Using O6-alkylguanine-DNA Alkyltransferases - Google Patents

Methods of Using O6-alkylguanine-DNA Alkyltransferases Download PDF

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Publication number
AU2002251257B2
AU2002251257B2 AU2002251257A AU2002251257A AU2002251257B2 AU 2002251257 B2 AU2002251257 B2 AU 2002251257B2 AU 2002251257 A AU2002251257 A AU 2002251257A AU 2002251257 A AU2002251257 A AU 2002251257A AU 2002251257 B2 AU2002251257 B2 AU 2002251257B2
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AU
Australia
Prior art keywords
agt
fusion protein
label
protein
alkylguanine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2002251257A
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English (en)
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AU2002251257A1 (en
Inventor
Susanne Gendreizig
Kai Johnsson
Antje Keppler
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Ecole Polytechnique Federale de Lausanne EPFL
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Ecole Polytechnique Federale de Lausanne EPFL
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Publication of AU2002251257A1 publication Critical patent/AU2002251257A1/en
Application granted granted Critical
Publication of AU2002251257B2 publication Critical patent/AU2002251257B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2002251257A 2001-04-10 2002-04-05 Methods of Using O6-alkylguanine-DNA Alkyltransferases Ceased AU2002251257B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28276601P 2001-04-10 2001-04-10
US60/282,766 2001-04-10
PCT/GB2002/001636 WO2002083937A2 (en) 2001-04-10 2002-04-05 Methods using o6-alkylguanine-dna alkyltransferases

Publications (2)

Publication Number Publication Date
AU2002251257A1 AU2002251257A1 (en) 2003-04-17
AU2002251257B2 true AU2002251257B2 (en) 2007-08-30

Family

ID=23083030

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002251257A Ceased AU2002251257B2 (en) 2001-04-10 2002-04-05 Methods of Using O6-alkylguanine-DNA Alkyltransferases

Country Status (14)

Country Link
US (2) US7939284B2 (enExample)
EP (4) EP2211177B1 (enExample)
JP (2) JP4195815B2 (enExample)
KR (1) KR20040039194A (enExample)
CN (2) CN1975422B (enExample)
AT (3) ATE331220T1 (enExample)
AU (1) AU2002251257B2 (enExample)
CA (1) CA2443570A1 (enExample)
DE (3) DE60237792D1 (enExample)
DK (1) DK1410023T3 (enExample)
IL (1) IL157880A0 (enExample)
NZ (3) NZ540410A (enExample)
WO (1) WO2002083937A2 (enExample)
ZA (1) ZA200307442B (enExample)

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EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
AU2004242249A1 (en) * 2003-05-23 2004-12-02 Epfl-Ecole Polytechnique Federale De Lausanne Methods for protein labeling based on acyl carrier protein
WO2005085431A2 (en) * 2004-03-02 2005-09-15 Epfl Ecole Polytechnique Federale De Lausanne Mutants of o6-alkylguanine-dna alkyltransferase
WO2005085470A1 (en) * 2004-03-02 2005-09-15 Epfl Ecole Polytechnique Federale De Lausanne Specific substrates for o6- alkylguanine-dna alkyltransferase
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7825096B2 (en) 2004-09-08 2010-11-02 The United States Of America As Represented By The Department Of Health And Human Services O6-alkylguanine-DNA alkyltransferase inactivators and beta-glucuronidase cleavable prodrugs
US7273714B2 (en) * 2005-03-11 2007-09-25 Vion Pharmaceuticals, Inc. Alkylguanyltransferase assays
WO2006114409A1 (en) * 2005-04-27 2006-11-02 Covalys Biosciences Ag Pyrimidines reacting with o6-alkylguanine-dna alkyltransferase
FR2890174B1 (fr) 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
FR2890446B1 (fr) 2005-09-05 2008-04-18 Cis Bio Internat Sa Methode de detection d'interaction intracellulaire entre bio-molecules
EP2044219B1 (en) 2006-06-30 2013-05-22 DiscoveRx Corporation Detectable nucleic acid tag
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
CN102443030B (zh) * 2006-12-05 2015-12-16 激光基因公司 光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EP1978093A1 (en) * 2007-04-04 2008-10-08 Ecole Polytechnique Fédérale de Lausanne (EPFL) Method of crosslinking two objects of interest
GB0711560D0 (en) * 2007-06-14 2007-07-25 Innova Biosciences Ltd Improvements in or relating to production of conjugates
WO2009058814A1 (en) * 2007-10-29 2009-05-07 New England Biolabs, Inc. Methods and compositions for detection and enrichment of target small rnas
EP2093564A1 (en) 2008-02-25 2009-08-26 Technische Universität Dresden Medizinische Fakultät Carl Gustav Carus Method for distinguishing secretory granules of different ages
US8148503B2 (en) 2008-06-11 2012-04-03 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in DNA sequencing
FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
DE112011100297B4 (de) 2010-01-20 2017-08-24 New England Biolabs, Inc. Zusammensetzungen, Verfahren und damit in Zusammenhang stehende Anwendungen zum Spalten modifizierter DNA
WO2012037361A2 (en) 2010-09-15 2012-03-22 Endacea, Inc. Methods of use and kit for measurement of lipopolysaccharide with a time resolved fluorescence based assay
CN104024269B (zh) 2011-09-13 2017-05-24 激光基因公司 5‑甲氧基，3’‑oh未阻断，可快速光切割的终止核苷酸以及用于核酸测序的方法
JP6215223B2 (ja) 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ
US9200260B2 (en) 2012-03-15 2015-12-01 New England Biolabs, Inc. Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
JP5781589B2 (ja) * 2013-12-20 2015-09-24 レーザーゲン インコーポレイテッド 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
US10080787B2 (en) 2014-07-02 2018-09-25 New England Biolabs, Inc. Universal N-glycan binding reagent
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
CN104844710B (zh) * 2015-04-13 2018-02-02 西北大学 定向固定化pega复合型树酯的制备方法及应用
WO2017214151A1 (en) 2016-06-06 2017-12-14 Northwestern University Fusion protein construct
CN113444046B (zh) 2016-07-20 2023-10-27 纳莹(上海)生物科技有限公司 一种荧光探针及其制备方法和用途
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN111253417B (zh) * 2019-09-04 2022-03-25 大连民族大学 一种嘌呤衍生物的合成及应用
EP4069731A4 (en) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
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CN112010983A (zh) * 2020-08-11 2020-12-01 西北大学 止咳平喘中药活性成分靶向筛选方法及试剂盒
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EP4347804A1 (en) 2021-05-27 2024-04-10 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
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WO2023197015A1 (en) 2022-04-08 2023-10-12 New England Biolabs, Inc. Compositions and analysis of dephosphorylated oligoribonucleotides
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US20250115953A1 (en) 2023-10-05 2025-04-10 New England Biolabs, Inc. Methylcytosine-Selective Deaminases and Uses Thereof
WO2025129193A1 (en) 2023-12-15 2025-06-19 New England Biolabs, Inc. Glycosyltransferases and related methods
WO2025137453A1 (en) 2023-12-20 2025-06-26 New England Biolabs, Inc. Compositions, methods, kits, and instruments for analyzing rna structure
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WO2025227034A1 (en) 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods

Citations (1)

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Publication number Priority date Publication date Assignee Title
DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

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ATE250060T1 (de) * 1993-06-08 2003-10-15 Cancer Rec Tech Ltd O6-substituierte guaninederivate, verfahren zu ihre herstellung und ihre anwendung für behandlung von tumorzellen

Patent Citations (1)

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DE19903895A1 (de) * 1999-02-01 2000-08-03 Kai Johnsson Selektive Immobilisierung und Nachweis der O·6·-Alkylguanin-DNA-Alkyltransferase in der aktiven Form

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Damoiseaux R et al, Chembiochem (2001), vol 2 (4): 285-287 *
Vaidyanathan G et al; Bioconjugate Chem (2000); vol 11: 868-875 *

Also Published As

Publication number Publication date
NZ540410A (en) 2006-06-30
DK1410023T3 (da) 2006-10-09
CN1527941A (zh) 2004-09-08
EP1410023A2 (en) 2004-04-21
EP2211177A1 (en) 2010-07-28
DE60212642T2 (de) 2007-06-14
US7939284B2 (en) 2011-05-10
ATE482395T1 (de) 2010-10-15
JP4226053B2 (ja) 2009-02-18
WO2002083937A2 (en) 2002-10-24
CA2443570A1 (en) 2002-10-24
CN1975422B (zh) 2012-09-05
JP2004532028A (ja) 2004-10-21
DE60230689D1 (de) 2009-02-12
HK1068406A1 (en) 2005-04-29
JP4195815B2 (ja) 2008-12-17
EP1696234A1 (en) 2006-08-30
US20040115130A1 (en) 2004-06-17
ATE331220T1 (de) 2006-07-15
NZ537939A (en) 2005-08-26
KR20040039194A (ko) 2004-05-10
ATE419378T1 (de) 2009-01-15
EP1410023B1 (en) 2006-06-21
EP2211177B1 (en) 2014-07-16
CN1295510C (zh) 2007-01-17
EP2037271A1 (en) 2009-03-18
IL157880A0 (en) 2004-03-28
US20110201514A1 (en) 2011-08-18
NZ528594A (en) 2005-07-29
WO2002083937A3 (en) 2004-02-26
JP2008154587A (ja) 2008-07-10
US8367361B2 (en) 2013-02-05
CN1975422A (zh) 2007-06-06
DE60237792D1 (de) 2010-11-04
ZA200307442B (en) 2004-06-30
DE60212642D1 (de) 2006-08-03
EP1696234B1 (en) 2008-12-31
EP2037271B1 (en) 2010-09-22

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: DELETE THE INVENTOR SIMON JOHN KIDDLE

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired