DE60220893T2 - Verfahren und gelzusammensetzungen zum einkapseln lebender zellen und organischer moleküle - Google Patents
Verfahren und gelzusammensetzungen zum einkapseln lebender zellen und organischer moleküle Download PDFInfo
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- DE60220893T2 DE60220893T2 DE60220893T DE60220893T DE60220893T2 DE 60220893 T2 DE60220893 T2 DE 60220893T2 DE 60220893 T DE60220893 T DE 60220893T DE 60220893 T DE60220893 T DE 60220893T DE 60220893 T2 DE60220893 T2 DE 60220893T2
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- isocyanate
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| US28126801P | 2001-04-03 | 2001-04-03 | |
| US281268P | 2001-04-03 | ||
| PCT/US2002/010411 WO2002081662A1 (en) | 2001-04-03 | 2002-04-02 | Methods and gel compositions for encapsulating living cells and organic molecules |
Publications (2)
| Publication Number | Publication Date |
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| DE60220893D1 DE60220893D1 (de) | 2007-08-09 |
| DE60220893T2 true DE60220893T2 (de) | 2008-03-06 |
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| DE60220893T Expired - Fee Related DE60220893T2 (de) | 2001-04-03 | 2002-04-02 | Verfahren und gelzusammensetzungen zum einkapseln lebender zellen und organischer moleküle |
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| DE (1) | DE60220893T2 (enExample) |
| IL (2) | IL158128A0 (enExample) |
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| WO2002094900A1 (en) * | 2001-05-21 | 2002-11-28 | Dow Global Technologies Inc. | Transparent polyurethane-hydrogel composition, method of making transparent polyurethane-hydrogel composition, and air-freshener application |
| WO2003050276A1 (en) * | 2001-12-05 | 2003-06-19 | Dow Global Technologies Inc. | Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications |
| CA2488429C (en) | 2002-06-05 | 2013-05-07 | Benjamin Eithan Reubinoff | Generation of neural stem cells from undifferentiated human embryonic stem cells |
| EP2127736A1 (en) * | 2003-04-10 | 2009-12-02 | The President and Fellows of Harvard College | Formation and control of fluidic species |
| US20060240548A1 (en) * | 2003-06-26 | 2006-10-26 | Mordechai Deutsch | Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof |
| US9200245B2 (en) | 2003-06-26 | 2015-12-01 | Seng Enterprises Ltd. | Multiwell plate |
| EP2662136A3 (en) | 2003-08-27 | 2013-12-25 | President and Fellows of Harvard College | Method for handling and mixing droplets |
| US20050112650A1 (en) * | 2003-10-20 | 2005-05-26 | Ciphergen Biosystems, Inc. | Reactive polyurethane-based polymers |
| US20060040377A1 (en) * | 2004-08-17 | 2006-02-23 | Biocept, Inc. | Protein microarrays |
| WO2006042132A2 (en) * | 2004-10-08 | 2006-04-20 | Georgia Tech Research Corporation | Microencapsulation of cells in hydrogels using electrostatic potentials |
| US7439062B2 (en) | 2004-12-23 | 2008-10-21 | Biocept, Inc. | Beads for capturing target cells from bodily fluid |
| US20090136982A1 (en) | 2005-01-18 | 2009-05-28 | Biocept, Inc. | Cell separation using microchannel having patterned posts |
| US8158410B2 (en) | 2005-01-18 | 2012-04-17 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
| CN103382434B (zh) | 2005-01-18 | 2016-05-25 | 生物概念股份有限公司 | 利用含排列成图案的立柱的微通道分离细胞 |
| WO2007030661A1 (en) * | 2005-09-09 | 2007-03-15 | Avery Dennison Corporation | Hydrogel including modified cyclodextrin crosslinked with polyurethane prepolymer |
| WO2007038982A1 (en) * | 2005-09-27 | 2007-04-12 | Universite Joseph Fourier - Grenoble 1 | Hydrogel functionalized with a polymerizable moiety and their uses as biosensors or bioreactors |
| US8263405B2 (en) | 2005-11-24 | 2012-09-11 | Mitsuru Akashi | Controllably degradable hydrogel for culturing cells to produce three-dimensionally organized cells |
| US7695956B2 (en) | 2006-01-12 | 2010-04-13 | Biocept, Inc. | Device for cell separation and analysis and method of using |
| KR100849185B1 (ko) * | 2006-01-19 | 2008-07-30 | 서울산업대학교 산학협력단 | 키토산 또는 히알루론산-폴리에틸렌옥사이드 및키토산-히알루론산-폴리에틸렌옥사이드를 기저로 하는하이드로젤과 이의 제조방법 |
| US20070243549A1 (en) * | 2006-04-12 | 2007-10-18 | Biocept, Inc. | Enrichment of circulating fetal dna |
| KR100721431B1 (ko) | 2006-04-19 | 2007-05-25 | 학교법인 포항공과대학교 | 고분자 캡슐 및 그 제조방법 |
| EP2086749B1 (en) | 2006-11-03 | 2013-05-08 | Trustees Of Tufts College | Nanopatterned biopolymer optical device and method of manufacturing the same |
| CA2704768A1 (en) | 2006-11-03 | 2008-10-23 | Trustees Of Tufts College | Biopolymer sensor and method of manufacturing the same |
| WO2008118211A2 (en) * | 2006-11-03 | 2008-10-02 | Trustees Of Tufts College | Biopolymer photonic crystals and method of manufacturing the same |
| WO2008127403A2 (en) * | 2006-11-03 | 2008-10-23 | Trustees Of Tufts College | Biopolymer optofluidic device and method of manufacturing the same |
| US20080176768A1 (en) * | 2007-01-23 | 2008-07-24 | Honeywell Honeywell International | Hydrogel microarray with embedded metal nanoparticles |
| CA2742844C (en) | 2007-11-05 | 2017-02-28 | Trustees Of Tufts College | Fabrication of silk fibroin photonic structures by nanocontact imprinting |
| WO2009081409A2 (en) | 2007-12-26 | 2009-07-02 | Seng Enterprises Ltd. | Device for the study of living cells |
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| CA2709107A1 (en) * | 2007-12-13 | 2009-06-18 | Danisco Us Inc. | Compositions and methods for producing isoprene |
| JP2011525254A (ja) * | 2008-06-18 | 2011-09-15 | トラスティーズ オブ タフツ カレッジ | 食用のホログラフィック絹製品 |
| JP5188314B2 (ja) * | 2008-08-04 | 2013-04-24 | キヤノン株式会社 | 生体高分子検査装置及びその方法 |
| US20100240054A1 (en) * | 2008-09-22 | 2010-09-23 | Biocept, Inc. | Identification and isolation of fetal cells and nucleic acid |
| CA2789009C (en) | 2009-02-12 | 2017-03-21 | Trustees Of Tufts College | Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications |
| JP2012533780A (ja) | 2009-07-20 | 2012-12-27 | タフツ ユニバーシティー／トラスティーズ オブ タフツ カレッジ | タンパク質のみからなる移植可能な吸収性反射体 |
| WO2011026101A2 (en) | 2009-08-31 | 2011-03-03 | Trustees Of Tufts College | Silk transistor devices |
| CN107033368A (zh) | 2009-11-09 | 2017-08-11 | 聚光灯技术合伙有限责任公司 | 碎裂水凝胶 |
| CA2780294C (en) | 2009-11-09 | 2018-01-16 | Spotlight Technology Partners Llc | Polysaccharide based hydrogels |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US9174216B2 (en) | 2013-03-13 | 2015-11-03 | DeNovo Science, Inc. | System for capturing and analyzing cells |
| CA2842359A1 (en) | 2011-08-01 | 2013-02-07 | Denovo Sciences | Cell capture system and method of use |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| EP2796873A1 (en) * | 2013-04-25 | 2014-10-29 | QGel SA | Method for a cell-based drug screening assay and the use thereof |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| JPWO2015147147A1 (ja) * | 2014-03-26 | 2017-04-13 | 国立大学法人北海道大学 | 生物学的試料をハイドロゲルで高速固定する方法及びそのための装置 |
| US9694338B2 (en) * | 2014-12-18 | 2017-07-04 | Wisconsin Alumni Research Foundation | Covalently-immobilized hydrogel arrays in multi-well plates |
| EP3403098B1 (en) | 2016-01-12 | 2021-09-22 | BioAtla, Inc. | Diagnostics using conditionally active antibodies |
| WO2019046307A1 (en) | 2017-08-29 | 2019-03-07 | Celsee Diagnostics, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11578322B2 (en) | 2019-05-07 | 2023-02-14 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| AU2020290981B2 (en) | 2019-06-14 | 2024-03-07 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| US20220340949A1 (en) | 2021-04-16 | 2022-10-27 | Capra Biosciences, Inc. | Methods of Isoprenoid Synthesis Using a Genetically Engineered Hydrocarbonoclastic Organism in a Biofilm Bioreactor |
| JP2024542006A (ja) * | 2021-10-29 | 2024-11-13 | シギロン セラピューティクス， インコーポレイテッド | 細胞ベースの療法のための組成物及び関連する方法 |
| EP4444910A4 (en) | 2021-12-10 | 2025-11-05 | Bio Rad Laboratories Inc | Compositions, processes and systems for the treatment of samples with functionalized particles of adjustable morphology |
| JP2025526687A (ja) | 2022-08-10 | 2025-08-15 | カプラ バイオサイエンシーズ インコーポレイテッド | 循環型バイオフィルムバイオリアクター |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4098645A (en) * | 1976-02-24 | 1978-07-04 | W. R. Grace & Co. | Immobilization of proteins with polyurethane polymers |
| US4436813A (en) * | 1982-03-16 | 1984-03-13 | Purification Engineering, Inc. | Immobilized microbial cell composition for making L-aspartic acid |
| US4794090A (en) * | 1986-09-26 | 1988-12-27 | W. R. Grace & Co.-Conn. | Immobilization support for biologicals |
| US5175229A (en) * | 1986-11-18 | 1992-12-29 | W. R. Grace & Co.-Conn. | Biocompatible polyurea-urethane hydrated polymers |
| US5403750A (en) * | 1991-03-06 | 1995-04-04 | W. R. Grace & Co.-Conn. | Biocompatible, low protein adsorption affinity matrix |
| US5573934A (en) * | 1992-04-20 | 1996-11-12 | Board Of Regents, The University Of Texas System | Gels for encapsulation of biological materials |
| JP3359640B2 (ja) * | 1995-02-01 | 2002-12-24 | シュナイダー（ユーエスエー）インク | 疎水性ポリマー類の親水性化方法 |
| EP0859051B1 (en) * | 1997-02-14 | 2002-01-16 | Nippon Shokubai Co., Ltd. | Immobilized biocatalyst |
| KR100694914B1 (ko) * | 1998-07-09 | 2007-03-14 | 바이오셉트 인코포레이티드 | 디엔에이 서열 하이브리드화를 최적화하기 위한 개선된펩티드 핵산 유니버셜 라이브러리의 사용방법 |
| US6818018B1 (en) * | 1998-08-14 | 2004-11-16 | Incept Llc | In situ polymerizable hydrogels |
| EP1025860B1 (de) * | 1999-01-29 | 2005-01-12 | Beiersdorf Aktiengesellschaft | Proteinhaltige Hydrogele |
| US6174683B1 (en) * | 1999-04-26 | 2001-01-16 | Biocept, Inc. | Method of making biochips and the biochips resulting therefrom |
| US6642046B1 (en) * | 1999-12-09 | 2003-11-04 | Motorola, Inc. | Method and apparatus for performing biological reactions on a substrate surface |
| JP2004518138A (ja) * | 2000-10-26 | 2004-06-17 | バイオセプト インコーポレイテッド | ３次元型バイオチップ |
| WO2003050276A1 (en) * | 2001-12-05 | 2003-06-19 | Dow Global Technologies Inc. | Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications |
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2002
- 2002-04-02 US US10/398,725 patent/US7172866B2/en not_active Expired - Lifetime
- 2002-04-02 EP EP02763911A patent/EP1373472B1/en not_active Expired - Lifetime
- 2002-04-02 CA CA002443060A patent/CA2443060A1/en not_active Abandoned
- 2002-04-02 JP JP2002580026A patent/JP4198468B2/ja not_active Expired - Fee Related
- 2002-04-02 WO PCT/US2002/010411 patent/WO2002081662A1/en not_active Ceased
- 2002-04-02 IL IL15812802A patent/IL158128A0/xx active IP Right Grant
- 2002-04-02 AT AT02763911T patent/ATE365792T1/de not_active IP Right Cessation
- 2002-04-02 DE DE60220893T patent/DE60220893T2/de not_active Expired - Fee Related
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2003
- 2003-09-25 IL IL158128A patent/IL158128A/en not_active IP Right Cessation
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2007
- 2007-02-01 US US11/670,061 patent/US20070141164A1/en not_active Abandoned
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|---|---|
| US7172866B2 (en) | 2007-02-06 |
| IL158128A (en) | 2009-02-11 |
| EP1373472A4 (en) | 2005-11-09 |
| ATE365792T1 (de) | 2007-07-15 |
| WO2002081662A1 (en) | 2002-10-17 |
| DE60220893D1 (de) | 2007-08-09 |
| JP4198468B2 (ja) | 2008-12-17 |
| US20070141164A1 (en) | 2007-06-21 |
| EP1373472A1 (en) | 2004-01-02 |
| JP2004533500A (ja) | 2004-11-04 |
| IL158128A0 (en) | 2004-03-28 |
| CA2443060A1 (en) | 2002-10-17 |
| EP1373472B1 (en) | 2007-06-27 |
| US20040029241A1 (en) | 2004-02-12 |
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