JP4198468B2 - 生細胞および有機分子をカプセル封入するための方法およびゲル組成物 - Google Patents

生細胞および有機分子をカプセル封入するための方法およびゲル組成物 Download PDF

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JP4198468B2
JP4198468B2 JP2002580026A JP2002580026A JP4198468B2 JP 4198468 B2 JP4198468 B2 JP 4198468B2 JP 2002580026 A JP2002580026 A JP 2002580026A JP 2002580026 A JP2002580026 A JP 2002580026A JP 4198468 B2 JP4198468 B2 JP 4198468B2
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hydrogel
biological material
isocyanate
prepolymer
thiol
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JP2004533500A (ja
JP2004533500A5 (enExample
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ハーン スーンカップ
ファグナニ ロベルト
ドン シャオファン
エフ．エドマン カール
ツィンベルグ パヴェル
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バイオセプト インコーポレイテッド
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605—Excipients; Inactive ingredients
    • A61K9/1629—Organic macromolecular compounds
    • A61K9/1641—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poloxamers
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G18/00—Polymeric products of isocyanates or isothiocyanates
    • C08G18/06—Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen
    • C08G18/08—Processes
    • C08G18/10—Prepolymer processes involving reaction of isocyanates or isothiocyanates with compounds having active hydrogen in a first reaction step
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
    • C08G65/32—Polymers modified by chemical after-treatment
    • C08G65/329—Polymers modified by chemical after-treatment with organic compounds
    • C08G65/333—Polymers modified by chemical after-treatment with organic compounds containing nitrogen
    • C08G65/33348—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing isocyanate group
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    • C08G65/334—Polymers modified by chemical after-treatment with organic compounds containing sulfur
    • C08G65/3342—Polymers modified by chemical after-treatment with organic compounds containing sulfur having sulfur bound to carbon and hydrogen
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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JP2002580026A 2001-04-03 2002-04-02 生細胞および有機分子をカプセル封入するための方法およびゲル組成物 Expired - Fee Related JP4198468B2 (ja)

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US28126801P 2001-04-03 2001-04-03
PCT/US2002/010411 WO2002081662A1 (en) 2001-04-03 2002-04-02 Methods and gel compositions for encapsulating living cells and organic molecules

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JP2004533500A JP2004533500A (ja) 2004-11-04
JP2004533500A5 JP2004533500A5 (enExample) 2005-12-22
JP4198468B2 true JP4198468B2 (ja) 2008-12-17

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US (2) US7172866B2 (enExample)
EP (1) EP1373472B1 (enExample)
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AT (1) ATE365792T1 (enExample)
CA (1) CA2443060A1 (enExample)
DE (1) DE60220893T2 (enExample)
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Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002094900A1 (en) * 2001-05-21 2002-11-28 Dow Global Technologies Inc. Transparent polyurethane-hydrogel composition, method of making transparent polyurethane-hydrogel composition, and air-freshener application
AU2002314790A1 (en) * 2001-12-05 2003-06-23 Dow Global Technologies Inc. Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications
CA2488429C (en) 2002-06-05 2013-05-07 Benjamin Eithan Reubinoff Generation of neural stem cells from undifferentiated human embryonic stem cells
EP2127736A1 (en) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Formation and control of fluidic species
US20060240548A1 (en) * 2003-06-26 2006-10-26 Mordechai Deutsch Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
JP4630870B2 (ja) 2003-08-27 2011-02-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ 流体種の電子的制御
US20050112650A1 (en) * 2003-10-20 2005-05-26 Ciphergen Biosystems, Inc. Reactive polyurethane-based polymers
US20060040377A1 (en) * 2004-08-17 2006-02-23 Biocept, Inc. Protein microarrays
WO2006042132A2 (en) * 2004-10-08 2006-04-20 Georgia Tech Research Corporation Microencapsulation of cells in hydrogels using electrostatic potentials
US7439062B2 (en) 2004-12-23 2008-10-21 Biocept, Inc. Beads for capturing target cells from bodily fluid
US8158410B2 (en) 2005-01-18 2012-04-17 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
ES2437845T3 (es) 2005-01-18 2014-01-14 Biocept, Inc. Separación de células usando un microcanal que tiene pilares con una configuración
US20090136982A1 (en) 2005-01-18 2009-05-28 Biocept, Inc. Cell separation using microchannel having patterned posts
EP1945675A1 (en) * 2005-09-09 2008-07-23 Avery Dennison Corporation Hydrogel including modified cyclodextrin crosslinked with polyurethane prepolymer
ES2377479T3 (es) * 2005-09-27 2012-03-28 Universite Joseph Fourier - Grenoble 1 Hidrogel funcionalizado con una porción polimerizable y sus utilizaciones como biosensores o biorreactores.
WO2007061058A1 (ja) * 2005-11-24 2007-05-31 Osaka University 刺激応答性分解ゲル
US7695956B2 (en) 2006-01-12 2010-04-13 Biocept, Inc. Device for cell separation and analysis and method of using
KR100849185B1 (ko) * 2006-01-19 2008-07-30 서울산업대학교 산학협력단 키토산 또는 히알루론산-폴리에틸렌옥사이드 및키토산-히알루론산-폴리에틸렌옥사이드를 기저로 하는하이드로젤과　 이의 제조방법
WO2007121276A2 (en) * 2006-04-12 2007-10-25 Biocept, Inc. Enrichment of circulating fetal dna
KR100721431B1 (ko) 2006-04-19 2007-05-25 학교법인 포항공과대학교 고분자 캡슐 및 그 제조방법
US8529835B2 (en) * 2006-11-03 2013-09-10 Tufts University Biopolymer sensor and method of manufacturing the same
WO2008118211A2 (en) 2006-11-03 2008-10-02 Trustees Of Tufts College Biopolymer photonic crystals and method of manufacturing the same
EP2650112B1 (en) 2006-11-03 2016-08-24 Trustees Of Tufts College Nanopatterned biopolymer optical device and method of manufacturing the same
US8195021B2 (en) 2006-11-03 2012-06-05 Tufts University/Trustees Of Tufts College Biopolymer optical waveguide and method of manufacturing the same
US20080176768A1 (en) * 2007-01-23 2008-07-24 Honeywell Honeywell International Hydrogel microarray with embedded metal nanoparticles
JP2011504421A (ja) 2007-11-05 2011-02-10 トラスティーズ オブ タフツ カレッジ ナノコンタクトインプリンティングによる絹フィブロインフォトニック構造の作製
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US9975118B2 (en) 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
AU2008334945C1 (en) * 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
US20110135697A1 (en) * 2008-06-18 2011-06-09 Trustees Of Tufts College Edible holographic silk products
JP5188314B2 (ja) * 2008-08-04 2013-04-24 キヤノン株式会社 生体高分子検査装置及びその方法
US20100240054A1 (en) * 2008-09-22 2010-09-23 Biocept, Inc. Identification and isolation of fetal cells and nucleic acid
WO2010126640A2 (en) 2009-02-12 2010-11-04 Trustees Of Tufts College Nanoimprinting of silk fibroin structures for biomedical and biophotonic applications
US9016875B2 (en) 2009-07-20 2015-04-28 Tufts University/Trustees Of Tufts College All-protein implantable, resorbable reflectors
EP2474054A4 (en) 2009-08-31 2013-03-13 Tufts University Trustees Of Tufts College SILK TRANSISTOR DEVICES
AU2010314994B2 (en) 2009-11-09 2016-10-06 Spotlight Technology Partners Llc Fragmented hydrogels
US9700650B2 (en) 2009-11-09 2017-07-11 Spotlight Technology Partners Llc Polysaccharide based hydrogels
ES2797448T3 (es) 2011-08-01 2020-12-02 Bio Rad Laboratories Sistema de captura de células
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9174216B2 (en) 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
EP2796873A1 (en) * 2013-04-25 2014-10-29 QGel SA Method for a cell-based drug screening assay and the use thereof
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
WO2015147147A1 (ja) * 2014-03-26 2015-10-01 国立大学法人北海道大学 生物学的試料をハイドロゲルで高速固定する方法及びそのための装置
US9694338B2 (en) * 2014-12-18 2017-07-04 Wisconsin Alumni Research Foundation Covalently-immobilized hydrogel arrays in multi-well plates
EP3403098B1 (en) 2016-01-12 2021-09-22 BioAtla, Inc. Diagnostics using conditionally active antibodies
WO2019046307A1 (en) 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
SG11202112151UA (en) 2019-05-07 2021-12-30 Bio Rad Laboratories System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
CN114302643B (zh) 2019-06-14 2024-02-27 伯乐实验室有限公司 用于自动化单细胞处理和分析的系统和方法
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
AU2022257089A1 (en) 2021-04-16 2023-10-26 Capra Biosciences, Inc. Methods of isoprenoid synthesis using a genetically engineered hydrocarbonoclastic organism in a biofilm bioreactor
IL312462A (en) * 2021-10-29 2024-06-01 Sigilon Therapeutics Inc Compositions for cell-based therapies and related methods
WO2023107539A1 (en) 2021-12-10 2023-06-15 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
JP2025526687A (ja) 2022-08-10 2025-08-15 カプラ バイオサイエンシーズ インコーポレイテッド 循環型バイオフィルムバイオリアクター

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098645A (en) * 1976-02-24 1978-07-04 W. R. Grace & Co. Immobilization of proteins with polyurethane polymers
US4436813A (en) * 1982-03-16 1984-03-13 Purification Engineering, Inc. Immobilized microbial cell composition for making L-aspartic acid
US4794090A (en) * 1986-09-26 1988-12-27 W. R. Grace & Co.-Conn. Immobilization support for biologicals
US5175229A (en) * 1986-11-18 1992-12-29 W. R. Grace & Co.-Conn. Biocompatible polyurea-urethane hydrated polymers
US5403750A (en) * 1991-03-06 1995-04-04 W. R. Grace & Co.-Conn. Biocompatible, low protein adsorption affinity matrix
US5573934A (en) * 1992-04-20 1996-11-12 Board Of Regents, The University Of Texas System Gels for encapsulation of biological materials
MX9705922A (es) * 1995-02-01 1997-10-31 Schneider Usa Inc Procedimientos para hidrofilizar polimeros hidrofobos.
EP0859051B1 (en) * 1997-02-14 2002-01-16 Nippon Shokubai Co., Ltd. Immobilized biocatalyst
KR100694914B1 (ko) * 1998-07-09 2007-03-14 바이오셉트 인코포레이티드 디엔에이 서열 하이브리드화를 최적화하기 위한 개선된펩티드 핵산 유니버셜 라이브러리의 사용방법
US6818018B1 (en) * 1998-08-14 2004-11-16 Incept Llc In situ polymerizable hydrogels
DE50009177D1 (de) * 1999-01-29 2005-02-17 Beiersdorf Ag Proteinhaltige Hydrogele
US6174683B1 (en) * 1999-04-26 2001-01-16 Biocept, Inc. Method of making biochips and the biochips resulting therefrom
US6642046B1 (en) * 1999-12-09 2003-11-04 Motorola, Inc. Method and apparatus for performing biological reactions on a substrate surface
DE60137523D1 (de) * 2000-10-26 2009-03-12 Biocept Inc Dreidimensionales format aufweisender biochip
AU2002314790A1 (en) * 2001-12-05 2003-06-23 Dow Global Technologies Inc. Method for immobilizing a biologic in a polyurethane-hydrogel composition, a composition prepared from the method, and biomedical applications

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IL158128A (en) 2009-02-11
US7172866B2 (en) 2007-02-06
IL158128A0 (en) 2004-03-28
EP1373472B1 (en) 2007-06-27
US20040029241A1 (en) 2004-02-12
EP1373472A4 (en) 2005-11-09
JP2004533500A (ja) 2004-11-04
WO2002081662A1 (en) 2002-10-17
ATE365792T1 (de) 2007-07-15
CA2443060A1 (en) 2002-10-17
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