DE60220018T2 - Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung - Google Patents

Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Download PDF

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Publication number
DE60220018T2
DE60220018T2 DE60220018T DE60220018T DE60220018T2 DE 60220018 T2 DE60220018 T2 DE 60220018T2 DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T DE60220018 T DE 60220018T DE 60220018 T2 DE60220018 T2 DE 60220018T2
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DE
Germany
Prior art keywords
dextran sulfate
hybridization
molecular weight
probe
tissue section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60220018T
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German (de)
English (en)
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DE60220018D1 (de
Inventor
Joseph Tucson UTERMOHLEN
Catherine Wolf
Kimberly Tucson CHRISTENSEN
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Publication date
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Publication of DE60220018D1 publication Critical patent/DE60220018D1/de
Publication of DE60220018T2 publication Critical patent/DE60220018T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Saccharide Compounds (AREA)
DE60220018T 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung Expired - Fee Related DE60220018T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US772123 2001-01-29
US09/772,123 US6656685B2 (en) 2001-01-29 2001-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use
PCT/US2002/002642 WO2002061139A2 (en) 2001-01-29 2002-01-29 Hybridization buffers using low molecular weight dextran sulfate and methods for their use

Publications (2)

Publication Number Publication Date
DE60220018D1 DE60220018D1 (de) 2007-06-21
DE60220018T2 true DE60220018T2 (de) 2008-01-10

Family

ID=25093987

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60220018T Expired - Fee Related DE60220018T2 (de) 2001-01-29 2002-01-29 Hybridisierungspuffer die niedermolekulares dextransulfat verwenden und methoden zu deren verwendung

Country Status (8)

Country Link
US (2) US6656685B2 (https=)
EP (1) EP1356116B1 (https=)
JP (1) JP2005503762A (https=)
AT (1) ATE361995T1 (https=)
AU (1) AU2002240180B2 (https=)
CA (1) CA2434854A1 (https=)
DE (1) DE60220018T2 (https=)
WO (1) WO2002061139A2 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002305300B2 (en) * 2001-04-30 2006-06-01 Ventana Medical Systems, Inc. Reagents and methods for automated in situ or microarray hybridization
WO2005033333A2 (en) 2003-10-07 2005-04-14 Dako Denmark A/S Methods and compositions for the diagnosis of cancer
DE102004028303A1 (de) * 2004-06-11 2005-12-29 Roche Diagnostics Gmbh Verfahren und Vorrichtung zum Nachweis von Analyten
EP2057289B1 (en) * 2006-08-09 2016-08-10 Univ Singapore PROCESS FOR MOLECULAR BIOLOGICAL APPLICATIONS
DK2444807T3 (da) 2006-11-01 2014-08-11 Ventana Med Syst Inc Mono- og dinitropyrazolhaptenkonjugater
EP2167963B1 (en) 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
JP5588970B2 (ja) 2008-05-27 2014-09-10 ダコ・デンマーク・エー／エス ハイブリダイゼーション組成物及び方法
WO2009149013A2 (en) 2008-06-05 2009-12-10 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
US9303287B2 (en) * 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
WO2011057017A1 (en) * 2009-11-06 2011-05-12 Bend Research, Inc. Aqueous nanoparticle suspensions for use in drug discovery
SG10201601853XA (en) 2011-03-11 2016-04-28 Univ Singapore Pericyte progenitors from peripheral blood
US10662465B2 (en) * 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
DK3133166T4 (da) * 2015-08-21 2023-03-20 42 Life Sciences Gmbh & Co Kg Sammensætning og fremgangsmåde til hybridisering
EP3351263A1 (en) 2017-01-20 2018-07-25 Universitätsklinikum Hamburg-Eppendorf Pharmaceutical preparation for treating or preventing tissue adhesion
DK3668972T3 (da) 2017-08-18 2025-01-13 Neutrolis Inc Konstruerede dnase-enzymer og anvendelse i terapi
SG11202100765UA (en) 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
US10988746B2 (en) 2018-10-08 2021-04-27 Neutrolis, Inc. Manufacturing and engineering of DNASE enzymes for therapy
IL325846A (en) 2018-10-08 2026-03-01 Neutrolis Inc Engineering deoxyribonuclease enzymes for manufacturing and medicine
US11058724B2 (en) 2018-10-08 2021-07-13 Neutrolis, Inc. Methods of using DNASE1-like 3 in therapy
US11352613B2 (en) 2019-02-04 2022-06-07 Neutrolis, Inc. Engineered human extracellular DNASE enzymes

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4689294A (en) 1984-11-19 1987-08-25 Miles Laboratories, Inc. Enhancement of hybridization of nucleic acids by anionic polymers
US5985549A (en) 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5273905A (en) * 1991-02-22 1993-12-28 Amoco Corporation Processing of slide mounted material
US5750340A (en) 1995-04-07 1998-05-12 University Of New Mexico In situ hybridization solution and process
US6127188A (en) * 1995-10-06 2000-10-03 Mj Research, Inc. Method and apparatus for controlling evaporation in histological procedures
US5763185A (en) * 1996-12-30 1998-06-09 Becton Dickinson And Company Method for reducing inhibitors of nucleic acid hybridization
JP3847559B2 (ja) * 1998-02-27 2006-11-22 ベンタナ・メデイカル・システムズ・インコーポレーテツド 独立スライド加熱器を有する自動化分子病理学装置
US6855552B2 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations

Also Published As

Publication number Publication date
ATE361995T1 (de) 2007-06-15
US6656685B2 (en) 2003-12-02
JP2005503762A (ja) 2005-02-10
CA2434854A1 (en) 2002-08-08
AU2002240180B2 (en) 2006-02-09
US20040253603A1 (en) 2004-12-16
DE60220018D1 (de) 2007-06-21
EP1356116B1 (en) 2007-05-09
US20020102554A1 (en) 2002-08-01
WO2002061139A2 (en) 2002-08-08
WO2002061139A3 (en) 2003-04-03
EP1356116A2 (en) 2003-10-29

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