DE60213256T2 - Verfahren zur bestimmung von mehrere analyten - Google Patents
Verfahren zur bestimmung von mehrere analyten Download PDFInfo
- Publication number
- DE60213256T2 DE60213256T2 DE60213256T DE60213256T DE60213256T2 DE 60213256 T2 DE60213256 T2 DE 60213256T2 DE 60213256 T DE60213256 T DE 60213256T DE 60213256 T DE60213256 T DE 60213256T DE 60213256 T2 DE60213256 T2 DE 60213256T2
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- nucleic acid
- acid sequence
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- specific
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01120466A EP1288308A1 (en) | 2001-08-28 | 2001-08-28 | A method for the determination of multiple analytes |
| EP01120466 | 2001-08-28 | ||
| PCT/EP2002/009496 WO2003020967A1 (en) | 2001-08-28 | 2002-08-24 | A method for the determination of multiple analytes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60213256D1 DE60213256D1 (de) | 2006-08-31 |
| DE60213256T2 true DE60213256T2 (de) | 2007-06-14 |
Family
ID=8178429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60213256T Expired - Lifetime DE60213256T2 (de) | 2001-08-28 | 2002-08-24 | Verfahren zur bestimmung von mehrere analyten |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20040191794A1 (enExample) |
| EP (2) | EP1288308A1 (enExample) |
| JP (1) | JP4559074B2 (enExample) |
| KR (1) | KR100653156B1 (enExample) |
| CN (1) | CN1575342A (enExample) |
| AT (1) | ATE333522T1 (enExample) |
| AU (1) | AU2002361779B2 (enExample) |
| BR (1) | BR0212265B1 (enExample) |
| DE (1) | DE60213256T2 (enExample) |
| DK (1) | DK1423541T3 (enExample) |
| ES (1) | ES2269804T3 (enExample) |
| MX (1) | MXPA04001730A (enExample) |
| PL (1) | PL210867B1 (enExample) |
| WO (1) | WO2003020967A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7385043B1 (en) * | 2003-04-30 | 2008-06-10 | The Public Health Research Institute Of The City Of New York, Inc. | Homogeneous multiplex screening assays and kits |
| JP4951505B2 (ja) * | 2004-05-07 | 2012-06-13 | セファイド | 生物学的因子の複合検出 |
| US7544792B2 (en) | 2004-07-13 | 2009-06-09 | Gen-Probe Incorporated | Compositions and methods for detection of hepatitis A virus nucleic acid |
| EP1930716A1 (de) * | 2006-12-08 | 2008-06-11 | Micronas GmbH | Verfahren und Vorrichtung zur Detektion eines Liganden in einem Fluid |
| US20110024043A1 (en) | 2009-07-02 | 2011-02-03 | Dexcom, Inc. | Continuous analyte sensors and methods of making same |
| BR112014011535A2 (pt) * | 2011-12-19 | 2017-05-09 | Hoffmann La Roche | método para a determinação in vitro e imunológica de presença da presença e/ou da quantidade de um parceiro de ligação, método in vitro e utilização de um anticorpo |
| EP4361607A3 (en) | 2012-02-03 | 2024-07-17 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| EP2880184B1 (en) | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| US10829816B2 (en) | 2012-11-19 | 2020-11-10 | Apton Biosystems, Inc. | Methods of analyte detection |
| WO2014170537A1 (en) | 2013-04-15 | 2014-10-23 | Wallac Oy | A method and a device for cross-talk correction of measured intensities |
| CN106574925B (zh) * | 2014-05-23 | 2019-07-26 | 萤火虫生物股份有限公司 | 用于生物测定的底物介导的反应器 |
| CN114921537A (zh) | 2017-03-17 | 2022-08-19 | 雅普顿生物系统公司 | 测序和高分辨率成像 |
| KR101871985B1 (ko) * | 2017-08-14 | 2018-06-27 | 고려대학교 산학협력단 | 3차원 단백질 나노입자 프로브 기반 복수 질환의 동시검출 방법 및 키트 |
| EP3781710A4 (en) | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS |
| CN108841939B (zh) * | 2018-06-21 | 2020-09-22 | 北京致雨生物科技有限公司 | 多重数字pcr浓度测量方法及微滴式数字pcr系统 |
| US12203129B2 (en) * | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| WO2020014388A1 (en) * | 2018-07-11 | 2020-01-16 | ChromaCode, Inc. | Methods for quantitation of analytes in multiplexed biochemical reactions |
| WO2020061237A1 (en) | 2018-09-19 | 2020-03-26 | Apton Biosystems, Inc. | Densely-packed analyte layers and detection methods |
| EP3899035B1 (en) * | 2018-12-20 | 2025-04-16 | F. Hoffmann-La Roche AG | Detection of target nucleic acid by solid-phase molography |
| US20220049303A1 (en) * | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| CN112485439B (zh) * | 2020-11-10 | 2023-07-18 | 深圳市科曼医疗设备有限公司 | 特定蛋白质反应检测方法、蛋白质检测装置和定标方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7224591A (en) * | 1990-01-24 | 1991-08-21 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | A rapid and sensitive test for detecting hepatitis a virus |
| US5968770A (en) * | 1995-02-10 | 1999-10-19 | Millennium Pharmaceuticals, Inc. | Compositions and methods for the treatment and diagnosis of cardiovascular disease using rchd523 as a target |
| US5759781A (en) * | 1995-12-22 | 1998-06-02 | Yale University | Multiparametric fluorescence in situ hybridization |
| US6007994A (en) * | 1995-12-22 | 1999-12-28 | Yale University | Multiparametric fluorescence in situ hybridization |
| JP2000507809A (ja) * | 1996-05-01 | 2000-06-27 | エール・ユニバーシティ | マルチパラメーター蛍光ｉｎ ｓｉｔｕハイブリダイゼーション |
| DE19723463A1 (de) * | 1997-06-04 | 1998-12-10 | Dade Behring Marburg Gmbh | Immunchemische Bestimmung von multivalenten Analyten |
| EP0953837A1 (en) * | 1998-05-01 | 1999-11-03 | F. Hoffmann-La Roche Ag | Fluorescence light measuring device and an apparatus wherein such a device is used |
| CA2329253A1 (en) * | 1998-06-02 | 1999-12-09 | Yale University | Multiparametric fluorescence in situ hybridization |
-
2001
- 2001-08-28 EP EP01120466A patent/EP1288308A1/en not_active Withdrawn
-
2002
- 2002-08-24 BR BRPI0212265-0B1A patent/BR0212265B1/pt not_active IP Right Cessation
- 2002-08-24 CN CNA028212398A patent/CN1575342A/zh active Pending
- 2002-08-24 ES ES02797620T patent/ES2269804T3/es not_active Expired - Lifetime
- 2002-08-24 AT AT02797620T patent/ATE333522T1/de active
- 2002-08-24 DE DE60213256T patent/DE60213256T2/de not_active Expired - Lifetime
- 2002-08-24 DK DK02797620T patent/DK1423541T3/da active
- 2002-08-24 EP EP02797620A patent/EP1423541B1/en not_active Expired - Lifetime
- 2002-08-24 PL PL369239A patent/PL210867B1/pl not_active IP Right Cessation
- 2002-08-24 JP JP2003525668A patent/JP4559074B2/ja not_active Expired - Fee Related
- 2002-08-24 WO PCT/EP2002/009496 patent/WO2003020967A1/en not_active Ceased
- 2002-08-24 AU AU2002361779A patent/AU2002361779B2/en not_active Ceased
- 2002-08-24 US US10/487,967 patent/US20040191794A1/en not_active Abandoned
- 2002-08-24 MX MXPA04001730A patent/MXPA04001730A/es active IP Right Grant
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| BR0212265B1 (pt) | 2013-11-19 |
| EP1423541A1 (en) | 2004-06-02 |
| JP2005501564A (ja) | 2005-01-20 |
| EP1288308A1 (en) | 2003-03-05 |
| WO2003020967A1 (en) | 2003-03-13 |
| ATE333522T1 (de) | 2006-08-15 |
| KR20040029076A (ko) | 2004-04-03 |
| US20040191794A1 (en) | 2004-09-30 |
| EP1423541B1 (en) | 2006-07-19 |
| MXPA04001730A (es) | 2004-05-31 |
| KR100653156B1 (ko) | 2006-12-01 |
| CN1575342A (zh) | 2005-02-02 |
| AU2002361779B2 (en) | 2007-01-25 |
| DE60213256D1 (de) | 2006-08-31 |
| BR0212265A (pt) | 2004-10-19 |
| DK1423541T3 (da) | 2006-10-30 |
| PL210867B1 (pl) | 2012-03-30 |
| JP4559074B2 (ja) | 2010-10-06 |
| ES2269804T3 (es) | 2007-04-01 |
| PL369239A1 (en) | 2005-04-18 |
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