DE60123251T2 - Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation - Google Patents

Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation Download PDF

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Publication number
DE60123251T2
DE60123251T2 DE60123251T DE60123251T DE60123251T2 DE 60123251 T2 DE60123251 T2 DE 60123251T2 DE 60123251 T DE60123251 T DE 60123251T DE 60123251 T DE60123251 T DE 60123251T DE 60123251 T2 DE60123251 T2 DE 60123251T2
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Prior art keywords
dna
endonuclease
bstnbi
gene
buffer
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English (en)
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DE60123251D1 (de
Inventor
Huimin Wenham Kong
Sears Lauren Rockport HIGGINS
A. Michael Manchester DALTON
B. Rebecca Hamilton KUCERA
Ira Cerrillos SCHILDKRAUT
G. Geoffrey Hamilton WILSON
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New England Biolabs Inc
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New England Biolabs Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60123251T 2000-06-02 2001-06-01 Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation Expired - Lifetime DE60123251T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/586,935 US6191267B1 (en) 2000-06-02 2000-06-02 Cloning and producing the N.BstNBI nicking endonuclease
US586935 2000-06-02
PCT/US2001/017804 WO2001094544A2 (en) 2000-06-02 2001-06-01 N.bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification

Publications (2)

Publication Number Publication Date
DE60123251D1 DE60123251D1 (de) 2006-11-02
DE60123251T2 true DE60123251T2 (de) 2007-05-10

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DE60123251T Expired - Lifetime DE60123251T2 (de) 2000-06-02 2001-06-01 Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation

Country Status (5)

Country Link
US (1) US6191267B1 (https=)
EP (2) EP1303530B1 (https=)
JP (1) JP4817587B2 (https=)
DE (1) DE60123251T2 (https=)
WO (1) WO2001094544A2 (https=)

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US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB0018120D0 (en) * 2000-07-24 2000-09-13 Fermentas Ab Nuclease
WO2002059357A2 (en) * 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US6391608B1 (en) * 2001-03-21 2002-05-21 New England Biolabs, Inc. Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli
US6395531B1 (en) * 2001-03-21 2002-05-28 New England Biolabs, Inc. Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli
US6395523B1 (en) 2001-06-01 2002-05-28 New England Biolabs, Inc. Engineering nicking endonucleases from type IIs restriction endonucleases
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
WO2003008642A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Amplification of nucleic acid fragments using nicking agents
US20030138800A1 (en) * 2001-07-15 2003-07-24 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
WO2003008624A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
JP4663988B2 (ja) * 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
EP1567669B1 (en) * 2002-12-02 2010-03-24 Illumina Cambridge Limited Determination of methylation of nucleic acid sequences
WO2004067726A2 (en) * 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
US20050136462A1 (en) 2003-12-19 2005-06-23 New England Biolabs, Inc. Method for engineering nicking enzymes
WO2006071770A2 (en) 2004-12-23 2006-07-06 I-Stat Corporation Molecular diagnostics system and methods
WO2007056173A1 (en) * 2005-11-09 2007-05-18 New England Biolabs, Inc. Genome amplification
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US20080050725A1 (en) * 2006-08-28 2008-02-28 Battelle Energy Alliance, Llc Methods of detecting DNA N-glycosylases, methods of determining N-glycosylase activity, and N-glycosylase assay kits
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2009000281A1 (en) * 2007-06-22 2008-12-31 Canvax Biotech, S.L. Methods for directional cloning of amplified nucleic acids and uses thereof
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2009140497A1 (en) * 2008-05-16 2009-11-19 New England Biolabs, Inc. Enzyme reagents for amplification of polynucleotides in the presence of inhibitors
EP2401388A4 (en) 2009-02-23 2012-12-05 Univ Georgetown SPECIFIC SEQUENCE SEQUENCE DETECTION OF NUCLEOTIDE SEQUENCES
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
CN102373284B (zh) * 2011-11-17 2012-12-26 山东出入境检验检疫局检验检疫技术中心 沙门氏菌的切刻内切酶核酸恒温扩增快速检测试剂盒
RU2601129C2 (ru) 2012-04-09 2016-10-27 Инвайролоджикс Инк. Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
US11198910B2 (en) 2016-09-02 2021-12-14 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
WO2019099081A1 (en) 2017-11-16 2019-05-23 New England Biolabs, Inc. Mapping the location, type and strand of damaged and/or mismatched nucleotides in double-stranded dna
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201915346D0 (en) 2019-06-21 2019-12-04 Lumiradx Tech Ltd Improvements in or relating to nicking enzymes
US11492667B2 (en) 2020-06-12 2022-11-08 New England Biolabs, Inc. Compositions and methods for detecting molecular targets on chromosomal DNA
US11667968B2 (en) 2021-05-27 2023-06-06 New England Biolabs, Inc. Fragmentation of DNA
WO2022261281A1 (en) 2021-06-09 2022-12-15 New England Biolabs, Inc. Target initiation and amplification of long dna with nuclease and replisome enzymes
WO2025104680A1 (en) 2023-11-17 2025-05-22 Friedrich Miescher Institute For Biomedical Research Dna accessibility for the assessment of male fertility

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US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
EP0618295B1 (en) 1986-06-06 1996-03-06 New England Biolabs, Inc. Method for cloning the DdeI restriction modification system
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5492823A (en) 1994-05-24 1996-02-20 New England Biolabs, Inc. Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
US6383770B1 (en) 1997-09-02 2002-05-07 New England Biolabs, Inc. Method for screening restriction endonucleases
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB2445030B (en) 2006-12-22 2009-06-17 Duncan Richard Louttit Improvements in graphic recognition devices

Also Published As

Publication number Publication date
JP2003535584A (ja) 2003-12-02
EP1303530B1 (en) 2006-09-20
US6191267B1 (en) 2001-02-20
WO2001094544A2 (en) 2001-12-13
WO2001094544A3 (en) 2002-04-04
DE60123251D1 (de) 2006-11-02
JP4817587B2 (ja) 2011-11-16
EP1757683A1 (en) 2007-02-28
EP1757683B1 (en) 2012-03-28
EP1303530A4 (en) 2004-10-20
EP1303530A2 (en) 2003-04-23

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