DE60123251D1 - Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation - Google Patents

Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation

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Publication number
DE60123251D1
DE60123251D1 DE60123251T DE60123251T DE60123251D1 DE 60123251 D1 DE60123251 D1 DE 60123251D1 DE 60123251 T DE60123251 T DE 60123251T DE 60123251 T DE60123251 T DE 60123251T DE 60123251 D1 DE60123251 D1 DE 60123251D1
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DE
Germany
Prior art keywords
nicking
nbi
bst
cloning
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60123251T
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English (en)
Other versions
DE60123251T2 (de
Inventor
Huimin Kong
Sears Higgins
A Dalton
B Kucera
Ira Schildkraut
G Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE60123251D1 publication Critical patent/DE60123251D1/de
Publication of DE60123251T2 publication Critical patent/DE60123251T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60123251T 2000-06-02 2001-06-01 Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation Expired - Lifetime DE60123251T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US586935 2000-06-02
US09/586,935 US6191267B1 (en) 2000-06-02 2000-06-02 Cloning and producing the N.BstNBI nicking endonuclease
PCT/US2001/017804 WO2001094544A2 (en) 2000-06-02 2001-06-01 N.bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification

Publications (2)

Publication Number Publication Date
DE60123251D1 true DE60123251D1 (de) 2006-11-02
DE60123251T2 DE60123251T2 (de) 2007-05-10

Family

ID=24347688

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60123251T Expired - Lifetime DE60123251T2 (de) 2000-06-02 2001-06-01 Klonierung und herstellung von n. i bst /i nbi nicking endonuclease und verwandte methoden zur verwendung von nicking endonucleasen in der einzelstrang-verdrängungsamplifikation

Country Status (5)

Country Link
US (1) US6191267B1 (de)
EP (2) EP1303530B1 (de)
JP (1) JP4817587B2 (de)
DE (1) DE60123251T2 (de)
WO (1) WO2001094544A2 (de)

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US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB0018120D0 (en) * 2000-07-24 2000-09-13 Fermentas Ab Nuclease
WO2002059357A2 (en) * 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
US6395531B1 (en) * 2001-03-21 2002-05-28 New England Biolabs, Inc. Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli
US6391608B1 (en) * 2001-03-21 2002-05-21 New England Biolabs, Inc. Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli
US6395523B1 (en) 2001-06-01 2002-05-28 New England Biolabs, Inc. Engineering nicking endonucleases from type IIs restriction endonucleases
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
CA2492220C (en) * 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
CA2492032A1 (en) * 2001-07-15 2003-08-14 Keck Graduate Institute Gene expression analysis using nicking agents
CA2492007A1 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Amplification of nucleic acid fragments using nicking agents
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
JP4663988B2 (ja) * 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
WO2004050915A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Determination of methylation of nucleic acid sequences
WO2004067726A2 (en) * 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1636337A4 (de) 2003-06-20 2007-07-04 Illumina Inc Verfahren und zusammensetzungen zur ganzgenomischen amplifikation und genotypisierung
US20050136462A1 (en) 2003-12-19 2005-06-23 New England Biolabs, Inc. Method for engineering nicking enzymes
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
JP5165383B2 (ja) 2004-12-23 2013-03-21 アイ−スタツト・コーポレイシヨン 分子診断システム及び方法
WO2010096202A2 (en) 2009-02-23 2010-08-26 Georgetown University Sequence-specific detection of nucleotide sequences
WO2007056173A1 (en) * 2005-11-09 2007-05-18 New England Biolabs, Inc. Genome amplification
US20090092967A1 (en) * 2006-06-26 2009-04-09 Epoch Biosciences, Inc. Method for generating target nucleic acid sequences
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US20080050725A1 (en) * 2006-08-28 2008-02-28 Battelle Energy Alliance, Llc Methods of detecting DNA N-glycosylases, methods of determining N-glycosylase activity, and N-glycosylase assay kits
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2009000281A1 (en) * 2007-06-22 2008-12-31 Canvax Biotech, S.L. Methods for directional cloning of amplified nucleic acids and uses thereof
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2009140497A1 (en) * 2008-05-16 2009-11-19 New England Biolabs, Inc. Enzyme reagents for amplification of polynucleotides in the presence of inhibitors
WO2013036929A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior Methods for obtaining a sequence
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
CN102373284B (zh) * 2011-11-17 2012-12-26 山东出入境检验检疫局检验检疫技术中心 沙门氏菌的切刻内切酶核酸恒温扩增快速检测试剂盒
CN114134207A (zh) 2012-04-09 2022-03-04 一龙公司 用于量化样品中核酸序列的组合物和方法
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
EP4219746A3 (de) 2016-09-02 2023-10-18 New England Biolabs, Inc. Analyse von chromatin unter verwendung eines nicking-enzyms
EP4276197A3 (de) 2017-11-16 2024-01-17 New England Biolabs, Inc. Abbildung von ort, typ und strang beschädigter nukleotide in doppelsträngiger dna
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
GB201915346D0 (en) 2019-06-21 2019-12-04 Lumiradx Tech Ltd Improvements in or relating to nicking enzymes
WO2021252498A1 (en) 2020-06-12 2021-12-16 New England Biolabs, Inc. Compositions and methods for detecting molecular targets on chromosomal dna
US11667968B2 (en) 2021-05-27 2023-06-06 New England Biolabs, Inc. Fragmentation of DNA
WO2022261281A1 (en) 2021-06-09 2022-12-15 New England Biolabs, Inc. Target initiation and amplification of long dna with nuclease and replisome enzymes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200333A (en) 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
JP2647847B2 (ja) 1986-06-06 1997-08-27 ニユ−・イングランド・バイオレイブス・インコ−ポレイテツド ＢａｍＨＩ制限修飾系のクローニング
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5492823A (en) 1994-05-24 1996-02-20 New England Biolabs, Inc. Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
WO1999011821A1 (en) 1997-09-02 1999-03-11 New England Biolabs, Inc. Method for screening restriction endonucleases
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB2445030B (en) 2006-12-22 2009-06-17 Duncan Richard Louttit Improvements in graphic recognition devices

Also Published As

Publication number Publication date
DE60123251T2 (de) 2007-05-10
EP1303530A2 (de) 2003-04-23
EP1757683B1 (de) 2012-03-28
JP4817587B2 (ja) 2011-11-16
EP1757683A1 (de) 2007-02-28
US6191267B1 (en) 2001-02-20
JP2003535584A (ja) 2003-12-02
WO2001094544A2 (en) 2001-12-13
WO2001094544A3 (en) 2002-04-04
EP1303530B1 (de) 2006-09-20
EP1303530A4 (de) 2004-10-20

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