JP4817587B2 - Ｎ．ＢｓｔＮＢＩ切断エンドヌクレアーゼのクローニング及び産生並びに一本鎖置換増幅における切断エンドヌクレアーゼ使用に関する方法 - Google Patents
Ｎ．ＢｓｔＮＢＩ切断エンドヌクレアーゼのクローニング及び産生並びに一本鎖置換増幅における切断エンドヌクレアーゼ使用に関する方法 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/586,935 US6191267B1 (en) | 2000-06-02 | 2000-06-02 | Cloning and producing the N.BstNBI nicking endonuclease |
| US09/586,935 | 2000-06-02 | ||
| PCT/US2001/017804 WO2001094544A2 (en) | 2000-06-02 | 2001-06-01 | N.bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003535584A JP2003535584A (ja) | 2003-12-02 |
| JP2003535584A5 JP2003535584A5 (https=) | 2008-05-22 |
| JP4817587B2 true JP4817587B2 (ja) | 2011-11-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002502087A Expired - Lifetime JP4817587B2 (ja) | 2000-06-02 | 2001-06-01 | Ｎ．ＢｓｔＮＢＩ切断エンドヌクレアーゼのクローニング及び産生並びに一本鎖置換増幅における切断エンドヌクレアーゼ使用に関する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6191267B1 (https=) |
| EP (2) | EP1303530B1 (https=) |
| JP (1) | JP4817587B2 (https=) |
| DE (1) | DE60123251T2 (https=) |
| WO (1) | WO2001094544A2 (https=) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6191267B1 (en) | 2000-06-02 | 2001-02-20 | New England Biolabs, Inc. | Cloning and producing the N.BstNBI nicking endonuclease |
| GB0018120D0 (en) * | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
| EP1423529B1 (en) * | 2001-01-24 | 2013-11-20 | Genomic Expression APS | Assay for analyzing gene expression |
| US6958217B2 (en) * | 2001-01-24 | 2005-10-25 | Genomic Expression Aps | Single-stranded polynucleotide tags |
| US6395531B1 (en) * | 2001-03-21 | 2002-05-28 | New England Biolabs, Inc. | Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli |
| US6391608B1 (en) * | 2001-03-21 | 2002-05-21 | New England Biolabs, Inc. | Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli |
| US6395523B1 (en) | 2001-06-01 | 2002-05-28 | New England Biolabs, Inc. | Engineering nicking endonucleases from type IIs restriction endonucleases |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| CN1656233A (zh) * | 2001-07-15 | 2005-08-17 | 凯克研究生院 | 利用切割剂扩增核酸片段 |
| CA2492423A1 (en) * | 2001-07-15 | 2004-03-18 | Keck Graduate Institute | Exponential amplification of nucleic acids using nicking agents |
| WO2003008624A2 (en) * | 2001-07-15 | 2003-01-30 | Keck Graduate Institute | Nucleic acid amplification using nicking agents |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| US7435572B2 (en) | 2002-04-12 | 2008-10-14 | New England Biolabs, Inc. | Methods and compositions for DNA manipulation |
| WO2004050915A1 (en) * | 2002-12-02 | 2004-06-17 | Solexa Limited | Determination of methylation of nucleic acid sequences |
| WO2004067726A2 (en) * | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Isothermal reactions for the amplification of oligonucleotides |
| US20050181394A1 (en) * | 2003-06-20 | 2005-08-18 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| US20040259100A1 (en) * | 2003-06-20 | 2004-12-23 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| CA2528577C (en) | 2003-06-20 | 2012-08-07 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| US7314714B2 (en) * | 2003-12-19 | 2008-01-01 | Affymetrix, Inc. | Method of oligonucleotide synthesis |
| US20050136462A1 (en) | 2003-12-19 | 2005-06-23 | New England Biolabs, Inc. | Method for engineering nicking enzymes |
| US8883487B2 (en) | 2004-12-23 | 2014-11-11 | Abbott Point Of Care Inc. | Molecular diagnostics system and methods |
| WO2007056173A1 (en) * | 2005-11-09 | 2007-05-18 | New England Biolabs, Inc. | Genome amplification |
| US20090092967A1 (en) * | 2006-06-26 | 2009-04-09 | Epoch Biosciences, Inc. | Method for generating target nucleic acid sequences |
| JP4918409B2 (ja) | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| US20080050725A1 (en) * | 2006-08-28 | 2008-02-28 | Battelle Energy Alliance, Llc | Methods of detecting DNA N-glycosylases, methods of determining N-glycosylase activity, and N-glycosylase assay kits |
| WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| WO2009000281A1 (en) * | 2007-06-22 | 2008-12-31 | Canvax Biotech, S.L. | Methods for directional cloning of amplified nucleic acids and uses thereof |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| WO2009140497A1 (en) * | 2008-05-16 | 2009-11-19 | New England Biolabs, Inc. | Enzyme reagents for amplification of polynucleotides in the presence of inhibitors |
| EP2401388A4 (en) | 2009-02-23 | 2012-12-05 | Univ Georgetown | SPECIFIC SEQUENCE SEQUENCE DETECTION OF NUCLEOTIDE SEQUENCES |
| GB2496016B (en) | 2011-09-09 | 2016-03-16 | Univ Leland Stanford Junior | Methods for obtaining a sequence |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| CN102373284B (zh) * | 2011-11-17 | 2012-12-26 | 山东出入境检验检疫局检验检疫技术中心 | 沙门氏菌的切刻内切酶核酸恒温扩增快速检测试剂盒 |
| UA116205C2 (uk) | 2012-04-09 | 2018-02-26 | Енвіролоджикс Інк. | Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку |
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| US11198910B2 (en) | 2016-09-02 | 2021-12-14 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| EP3710596B1 (en) | 2017-11-16 | 2023-08-02 | New England Biolabs, Inc. | Mapping the location, type and strand of damaged nucleotides in double-stranded dna |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| GB201915346D0 (en) | 2019-06-21 | 2019-12-04 | Lumiradx Tech Ltd | Improvements in or relating to nicking enzymes |
| US20230123635A1 (en) | 2020-06-12 | 2023-04-20 | New England Biolabs, Inc. | Compositions and Methods for Detecting Molecular Targets on Chromosomal DNA |
| US11667968B2 (en) | 2021-05-27 | 2023-06-06 | New England Biolabs, Inc. | Fragmentation of DNA |
| WO2022261281A1 (en) | 2021-06-09 | 2022-12-15 | New England Biolabs, Inc. | Target initiation and amplification of long dna with nuclease and replisome enzymes |
| WO2025104680A1 (en) | 2023-11-17 | 2025-05-22 | Friedrich Miescher Institute For Biomedical Research | Dna accessibility for the assessment of male fertility |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5200333A (en) | 1985-03-01 | 1993-04-06 | New England Biolabs, Inc. | Cloning restriction and modification genes |
| EP0618295B1 (en) | 1986-06-06 | 1996-03-06 | New England Biolabs, Inc. | Method for cloning the DdeI restriction modification system |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5492823A (en) | 1994-05-24 | 1996-02-20 | New England Biolabs, Inc. | Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli |
| WO1999011821A1 (en) | 1997-09-02 | 1999-03-11 | New England Biolabs, Inc. | Method for screening restriction endonucleases |
| US6191267B1 (en) | 2000-06-02 | 2001-02-20 | New England Biolabs, Inc. | Cloning and producing the N.BstNBI nicking endonuclease |
| GB2445030B (en) | 2006-12-22 | 2009-06-17 | Duncan Richard Louttit | Improvements in graphic recognition devices |
-
2000
- 2000-06-02 US US09/586,935 patent/US6191267B1/en not_active Expired - Lifetime
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2001
- 2001-06-01 DE DE60123251T patent/DE60123251T2/de not_active Expired - Lifetime
- 2001-06-01 EP EP01939836A patent/EP1303530B1/en not_active Expired - Lifetime
- 2001-06-01 JP JP2002502087A patent/JP4817587B2/ja not_active Expired - Lifetime
- 2001-06-01 EP EP06076525A patent/EP1757683B1/en not_active Expired - Lifetime
- 2001-06-01 WO PCT/US2001/017804 patent/WO2001094544A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE60123251T2 (de) | 2007-05-10 |
| EP1757683B1 (en) | 2012-03-28 |
| WO2001094544A2 (en) | 2001-12-13 |
| EP1303530A4 (en) | 2004-10-20 |
| US6191267B1 (en) | 2001-02-20 |
| EP1303530B1 (en) | 2006-09-20 |
| DE60123251D1 (de) | 2006-11-02 |
| EP1757683A1 (en) | 2007-02-28 |
| EP1303530A2 (en) | 2003-04-23 |
| JP2003535584A (ja) | 2003-12-02 |
| WO2001094544A3 (en) | 2002-04-04 |
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