DE60121923T2 - Verfahren zur erfassung von analyten, die aus oberflächengebundenen liganden eluiert werden - Google Patents
Verfahren zur erfassung von analyten, die aus oberflächengebundenen liganden eluiert werden Download PDFInfo
- Publication number
- DE60121923T2 DE60121923T2 DE60121923T DE60121923T DE60121923T2 DE 60121923 T2 DE60121923 T2 DE 60121923T2 DE 60121923 T DE60121923 T DE 60121923T DE 60121923 T DE60121923 T DE 60121923T DE 60121923 T2 DE60121923 T2 DE 60121923T2
- Authority
- DE
- Germany
- Prior art keywords
- analyte
- analytes
- bound
- intercepted
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003446 ligand Substances 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 47
- 239000007787 solid Substances 0.000 claims abstract description 46
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 239000000463 material Substances 0.000 claims abstract description 41
- 238000004458 analytical method Methods 0.000 claims abstract description 17
- 239000012491 analyte Substances 0.000 claims description 96
- 239000011324 bead Substances 0.000 claims description 26
- 239000012530 fluid Substances 0.000 claims description 21
- 230000002000 scavenging effect Effects 0.000 claims description 17
- 230000003993 interaction Effects 0.000 claims description 16
- 108091033319 polynucleotide Proteins 0.000 claims description 16
- 102000040430 polynucleotide Human genes 0.000 claims description 16
- 239000002157 polynucleotide Substances 0.000 claims description 16
- 239000002516 radical scavenger Substances 0.000 claims description 15
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 8
- 239000000427 antigen Substances 0.000 claims description 8
- 102000036639 antigens Human genes 0.000 claims description 8
- 108091007433 antigens Proteins 0.000 claims description 8
- 238000010494 dissociation reaction Methods 0.000 claims description 8
- 230000005593 dissociations Effects 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 7
- 238000009825 accumulation Methods 0.000 claims description 6
- 150000002632 lipids Chemical class 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 108050001427 Avidin/streptavidin Proteins 0.000 claims description 4
- 102000004856 Lectins Human genes 0.000 claims description 4
- 108090001090 Lectins Proteins 0.000 claims description 4
- 102000018697 Membrane Proteins Human genes 0.000 claims description 4
- 108010052285 Membrane Proteins Proteins 0.000 claims description 4
- 230000002378 acidificating effect Effects 0.000 claims description 4
- 229960002685 biotin Drugs 0.000 claims description 4
- 235000020958 biotin Nutrition 0.000 claims description 4
- 239000011616 biotin Substances 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 4
- 229940088597 hormone Drugs 0.000 claims description 4
- 108091008039 hormone receptors Proteins 0.000 claims description 4
- 239000002523 lectin Substances 0.000 claims description 4
- 102000019758 lipid binding proteins Human genes 0.000 claims description 4
- 108020003175 receptors Proteins 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 108091008324 binding proteins Proteins 0.000 claims description 3
- 239000002738 chelating agent Substances 0.000 claims description 3
- 229920000936 Agarose Polymers 0.000 claims description 2
- 230000027455 binding Effects 0.000 claims description 2
- 239000012501 chromatography medium Substances 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 229920001477 hydrophilic polymer Polymers 0.000 claims description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 2
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 claims description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 101710129138 ATP synthase subunit 9, mitochondrial Proteins 0.000 claims 2
- 101710168506 ATP synthase subunit C, plastid Proteins 0.000 claims 2
- 101710114069 ATP synthase subunit c Proteins 0.000 claims 2
- 101710197943 ATP synthase subunit c, chloroplastic Proteins 0.000 claims 2
- 101710187091 ATP synthase subunit c, sodium ion specific Proteins 0.000 claims 2
- 239000002532 enzyme inhibitor Substances 0.000 claims 2
- 229940125532 enzyme inhibitor Drugs 0.000 claims 2
- 102000014914 Carrier Proteins Human genes 0.000 claims 1
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 14
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 7
- 235000019253 formic acid Nutrition 0.000 description 7
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
- 230000008929 regeneration Effects 0.000 description 6
- 238000011069 regeneration method Methods 0.000 description 6
- 239000003112 inhibitor Substances 0.000 description 5
- 238000004949 mass spectrometry Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 108010010369 HIV Protease Proteins 0.000 description 2
- -1 Lewis acid Lewis base Chemical class 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 230000009830 antibody antigen interaction Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 102000023732 binding proteins Human genes 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 238000000572 ellipsometry Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 108091016323 lipid binding proteins Proteins 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 238000010897 surface acoustic wave method Methods 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- CDUQMGQIHYISOP-RMKNXTFCSA-N (e)-2-cyano-3-phenylprop-2-enoic acid Chemical compound OC(=O)C(\C#N)=C\C1=CC=CC=C1 CDUQMGQIHYISOP-RMKNXTFCSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 239000002841 Lewis acid Substances 0.000 description 1
- 239000002879 Lewis base Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000000105 evaporative light scattering detection Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hcl hcl Chemical compound Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000009652 hydrodynamic focusing Methods 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000001906 matrix-assisted laser desorption--ionisation mass spectrometry Methods 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 238000012483 real time interaction analysis Methods 0.000 description 1
- 238000010223 real-time analysis Methods 0.000 description 1
- 238000001028 reflection method Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 description 1
- PCMORTLOPMLEFB-UHFFFAOYSA-N sinapinic acid Natural products COC1=CC(C=CC(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19033600P | 2000-03-16 | 2000-03-16 | |
| US190336P | 2000-03-16 | ||
| PCT/SE2001/000531 WO2001069258A1 (en) | 2000-03-16 | 2001-03-14 | Method for capturing analytes eluted from surface-bound ligands |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60121923D1 DE60121923D1 (de) | 2006-09-14 |
| DE60121923T2 true DE60121923T2 (de) | 2006-11-30 |
Family
ID=22700919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60121923T Expired - Lifetime DE60121923T2 (de) | 2000-03-16 | 2001-03-14 | Verfahren zur erfassung von analyten, die aus oberflächengebundenen liganden eluiert werden |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6503760B2 (https=) |
| EP (1) | EP1264179B1 (https=) |
| JP (1) | JP4860085B2 (https=) |
| AT (1) | ATE335202T1 (https=) |
| AU (1) | AU2001242928A1 (https=) |
| DE (1) | DE60121923T2 (https=) |
| WO (1) | WO2001069258A1 (https=) |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4860085B2 (ja) * | 2000-03-16 | 2012-01-25 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 表面結合リガンドから溶出された分析物を捕捉するための方法 |
| US7126688B2 (en) * | 2000-07-11 | 2006-10-24 | Maven Technologies, Llc | Microarray scanning |
| US6833920B2 (en) | 2000-07-11 | 2004-12-21 | Maven Technologies Llc | Apparatus and method for imaging |
| US7518724B2 (en) * | 2000-07-11 | 2009-04-14 | Maven Technologies | Image acquisition, processing, and display |
| US6594011B1 (en) | 2000-07-11 | 2003-07-15 | Maven Technologies, Llc | Imaging apparatus and method |
| US7193711B2 (en) * | 2000-07-11 | 2007-03-20 | Maven Technologies, Llc | Imaging method and apparatus |
| US7023547B2 (en) * | 2000-07-11 | 2006-04-04 | Maven Technologies, Llc | Apparatus including a biochip for imaging of biological samples and method |
| CA2474469A1 (en) * | 2002-01-28 | 2003-08-07 | Mds Proteomics, Inc. | Chemical proteomics |
| US20030166007A1 (en) * | 2002-01-28 | 2003-09-04 | Mds Proteomics Inc. | Chemical proteomics |
| AU2003231731A1 (en) * | 2002-04-15 | 2003-11-03 | American National Red Cross | Method for detecting ligands and targets in a mixture |
| US6821744B2 (en) * | 2002-10-29 | 2004-11-23 | Roche Diagnostics Operations, Inc. | Method, assay, and kit for quantifying HIV protease inhibitors |
| JPWO2004051231A1 (ja) * | 2002-11-29 | 2006-04-06 | 日本電気株式会社 | 分離装置および分離方法 |
| US7736909B2 (en) | 2003-01-09 | 2010-06-15 | Board Of Regents, The University Of Texas System | Methods and compositions comprising capture agents |
| US7483140B1 (en) | 2004-12-10 | 2009-01-27 | University Of Central Florida Research Foundation, Inc. | Micro integrated planar optical waveguide type SPR sensor |
| US7611908B2 (en) | 2005-05-02 | 2009-11-03 | Bioscale, Inc. | Method and apparatus for therapeutic drug monitoring using an acoustic device |
| US7300631B2 (en) * | 2005-05-02 | 2007-11-27 | Bioscale, Inc. | Method and apparatus for detection of analyte using a flexural plate wave device and magnetic particles |
| US7648844B2 (en) * | 2005-05-02 | 2010-01-19 | Bioscale, Inc. | Method and apparatus for detection of analyte using an acoustic device |
| US7749445B2 (en) * | 2005-05-02 | 2010-07-06 | Bioscale, Inc. | Method and apparatus for analyzing bioprocess fluids |
| JP2006313091A (ja) * | 2005-05-06 | 2006-11-16 | Fuji Photo Film Co Ltd | 結合物質回収方法及び装置 |
| EP1943500A4 (en) | 2005-09-30 | 2013-01-16 | Fujifilm Corp | DETECTION SYSTEM |
| JP4768417B2 (ja) * | 2005-11-28 | 2011-09-07 | 富士フイルム株式会社 | バイオセンサー |
| US7867783B2 (en) * | 2007-02-22 | 2011-01-11 | Maven Technologies, Llc | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates |
| US7863037B1 (en) | 2007-04-04 | 2011-01-04 | Maven Technologies, Llc | Ligand binding assays on microarrays in closed multiwell plates |
| US20090041633A1 (en) * | 2007-05-14 | 2009-02-12 | Dultz Shane C | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates |
| US7799558B1 (en) | 2007-05-22 | 2010-09-21 | Dultz Shane C | Ligand binding assays on microarrays in closed multiwell plates |
| US8354280B2 (en) * | 2007-09-06 | 2013-01-15 | Bioscale, Inc. | Reusable detection surfaces and methods of using same |
| US7981664B1 (en) | 2008-05-22 | 2011-07-19 | Maven Technologies, Llc | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates |
| US8039270B2 (en) * | 2008-05-22 | 2011-10-18 | Maven Technologies, Llc | Apparatus and method for performing ligand binding assays on microarrays in multiwell plates |
| EP2128617A1 (en) * | 2008-05-27 | 2009-12-02 | Koninklijke Philips Electronics N.V. | Device and methods for detecting analytes in saliva |
| BRPI1013012A2 (pt) | 2009-06-02 | 2016-10-18 | Opko Health Inc | identificação de pequenas moléculas reconhecidas por anticorpos em indivíduos com doenças neurodegenerativas |
| WO2011047257A1 (en) | 2009-10-16 | 2011-04-21 | The Board Of Regents Of The University Of Texas System | Compositions and methods for producing cyclic peptoid libraries |
| US8355133B2 (en) * | 2009-12-30 | 2013-01-15 | Maven Technologies, Llc | Biological testing with sawtooth-shaped prisms |
| US9050595B2 (en) | 2010-12-03 | 2015-06-09 | Abbott Point Of Care Inc. | Assay devices with integrated sample dilution and dilution verification and methods of using same |
| CA2821711C (en) | 2010-12-15 | 2017-10-10 | Baxter International Inc. | Eluate collection using conductivity gradient |
| CN108780081B (zh) | 2015-08-10 | 2021-04-09 | Essenlix公司 | 步骤简化、小样品、快速、易使用的生物/化学分析装置和方法 |
| WO2017048871A1 (en) | 2015-09-14 | 2017-03-23 | Essenlix Corp. | Device and system for analyzing a sample, particularly blood, as well as methods of using the same |
| CN111426822B (zh) | 2015-09-14 | 2022-12-13 | 艾森利克斯公司 | 采集分析蒸汽凝析，特别是呼出气凝析的装置与系统，以及使用方法 |
| US10628693B2 (en) | 2016-12-21 | 2020-04-21 | Essenlix Corporation | Devices and methods for authenticating a sample and use of the same |
| WO2018148342A1 (en) | 2017-02-07 | 2018-08-16 | Essenlix Corporation | Compressed open flow assay and use |
| CN111656155B (zh) | 2017-02-08 | 2024-05-31 | 上海宜晟生物科技有限公司 | 用于延迟分析的样品采集和操作 |
| US12151246B2 (en) | 2017-02-08 | 2024-11-26 | Essenlix Corporation | Molecular manipulation and assay with controlled temperature |
| US11927560B2 (en) | 2017-02-08 | 2024-03-12 | Essenlix Corporation | Bio/chemical material extraction and assay |
| CN110998324B (zh) | 2017-02-08 | 2025-03-28 | 上海宜晟生物科技有限公司 | 数字测定 |
| CA3052809A1 (en) | 2017-02-08 | 2018-08-23 | Essenlix Corporation | Qmax assays and applications |
| EP3662259A4 (en) | 2017-02-09 | 2021-08-25 | Essenlix Corporation | COLORIMETRIC TESTS |
| CN111433606B (zh) | 2017-02-09 | 2024-05-24 | 上海宜晟生物科技有限公司 | 采用不同间距高度的测定 |
| CN110998325B (zh) | 2017-02-09 | 2024-08-16 | 上海宜晟生物科技有限公司 | 扩增测定 |
| JP2020508043A (ja) | 2017-02-15 | 2020-03-19 | エッセンリックス コーポレーション | 急速な温度変化を伴うアッセイ |
| US11523752B2 (en) | 2017-02-16 | 2022-12-13 | Essenlix Corporation | Assay for vapor condensates |
| EP3638421A4 (en) | 2017-06-12 | 2021-04-07 | Essenlix Corporation | HOMOGENOUS ASSAY |
| CN120668916A (zh) | 2017-08-01 | 2025-09-19 | 上海宜晟生物科技有限公司 | 检查药物对微生物影响的装置和方法 |
| US11280706B2 (en) | 2017-08-01 | 2022-03-22 | Essenlix Corporation | Dilution calibration |
| WO2019028123A1 (en) | 2017-08-01 | 2019-02-07 | Essenlix Corporation | COLLECTION, MAINTENANCE AND DETERMINATION OF SAMPLES |
| WO2019075244A1 (en) | 2017-10-11 | 2019-04-18 | Essenlix Corporation | CONTAINING A LIQUID SAMPLE |
| WO2019075415A1 (en) | 2017-10-13 | 2019-04-18 | Essenlix Corporation | DEVICES AND METHODS FOR AUTHENTICATING MEDICAL ANALYSIS AND USES THEREOF |
| US11609224B2 (en) | 2017-10-26 | 2023-03-21 | Essenlix Corporation | Devices and methods for white blood cell analyses |
| US10807095B2 (en) | 2017-10-26 | 2020-10-20 | Essenlix Corporation | Making and tracking assay card |
| US11237113B2 (en) | 2017-10-26 | 2022-02-01 | Essenlix Corporation | Rapid pH measurement |
| CN107899552B (zh) * | 2017-10-31 | 2020-06-30 | 苏州博进生物技术有限公司 | 一种以磁性聚合物微球为基质的金属螯合亲和层析介质 |
| US11648551B2 (en) | 2017-12-12 | 2023-05-16 | Essenlix Corporation | Sample manipulation and assay with rapid temperature change |
| CN112534259A (zh) | 2017-12-14 | 2021-03-19 | Essenlix公司 | 监测毛发的装置，系统和方法 |
| WO2019140334A1 (en) | 2018-01-11 | 2019-07-18 | Essenlix Corporation | Homogeneous assay (ii) |
| EP3775834A4 (en) * | 2018-07-09 | 2021-03-17 | Hewlett-Packard Development Company, L.P. | ANALYTIC TRAP DEVICES WITH FLUID EJECTOR DEVICES |
| US11885952B2 (en) | 2018-07-30 | 2024-01-30 | Essenlix Corporation | Optics, device, and system for assaying and imaging |
| WO2020225971A1 (ja) * | 2019-05-08 | 2020-11-12 | 株式会社日立ハイテク | 自動分析装置の前処理方法 |
| EP4089401B1 (de) * | 2021-05-10 | 2024-11-06 | Siemens Aktiengesellschaft | Messeinrichtung und verfahren zur messung von mindestens zwei verschiedenen komponenten eines fluids mittels ramanstreuung und chemilumineszenz |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5236826A (en) * | 1985-12-10 | 1993-08-17 | Murex Corporation | Immunoassay for the detection or quantitation of an analyte |
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5236849A (en) * | 1987-08-11 | 1993-08-17 | Eiji Ishikawa | Method of high sensitivity immunoassay |
| GB8822180D0 (en) * | 1988-09-21 | 1988-10-26 | Glaverbel | Separation of product of biological process from fluid medium |
| JPH0466871A (ja) * | 1990-07-07 | 1992-03-03 | Eiji Ishikawa | 高感度な免疫測定法 |
| US5312730A (en) * | 1992-05-27 | 1994-05-17 | Ciba Corning Diagnostics Corp. | Immune complex transfer with lypophilic bridge |
| SE9201976D0 (sv) * | 1992-06-26 | 1992-06-26 | Tektolit Ab Haakan Eriksson | En metod foer att adsorbera proteiner och peptider till hydrofoba zeoliter |
| JPH08503698A (ja) * | 1992-10-02 | 1996-04-23 | ザ ゼネラル ホスピタル コーポレーション | アミノ酸模倣物を溶出試薬として用いる親和精製方法 |
| US5395587A (en) * | 1993-07-06 | 1995-03-07 | Smithkline Beecham Corporation | Surface plasmon resonance detector having collector for eluted ligate |
| US5445936A (en) * | 1993-09-15 | 1995-08-29 | Ciba Corning Diagnostics Corp. | Method for non-competitive binding assays |
| DE69422604T2 (de) * | 1994-03-08 | 2000-06-08 | Zeptosens Ag, Witterwil | Vorrichtung und Verfahren, die Bioaffinitätsassay und elektrophoretische Auftrennung kombinieren |
| JPH08178926A (ja) * | 1994-10-25 | 1996-07-12 | Sumitomo Pharmaceut Co Ltd | イムノアッセイプレートおよびその用途 |
| AU4117696A (en) * | 1994-11-24 | 1996-06-17 | Unipath Limited | Recovery of and uses of specific binding agents |
| JPH08304397A (ja) * | 1995-05-11 | 1996-11-22 | Sumitomo Pharmaceut Co Ltd | 病原体感染の検出方法 |
| US5756717A (en) * | 1995-05-24 | 1998-05-26 | Perseptive Biosystems, Inc | Protein imaging |
| GB9518429D0 (en) * | 1995-09-08 | 1995-11-08 | Pharmacia Biosensor | A rapid method for providing kinetic and structural data in molecular interaction analysis |
| CA2245714A1 (en) * | 1996-02-09 | 1997-08-14 | Martin Thomas French | Assay apparatus |
| WO1997044664A1 (en) * | 1996-05-23 | 1997-11-27 | Unilever Plc | Improvements in or relating to specific binding assays |
| ATE317979T1 (de) * | 1996-12-19 | 2006-03-15 | Dade Behring Marburg Gmbh | Immundissoziation zur verbesserung der immunchemischen bestimmung eines analyten |
| US6025197A (en) * | 1997-03-19 | 2000-02-15 | Zymogenetics, Inc. | Secreted salivary zsig32 polypeptides |
| US6289286B1 (en) * | 1998-05-29 | 2001-09-11 | Biacore Ab | Surface regeneration of biosensors and characterization of biomolecules associated therewith |
| JP3829491B2 (ja) * | 1998-08-27 | 2006-10-04 | 株式会社日立製作所 | プローブチップ、プローブチップ作成方法、試料検出方法、及び試料検出装置 |
| US6127129A (en) * | 1999-05-04 | 2000-10-03 | Wisconsin Alumni Research Foundation | Process to create biomolecule and/or cellular arrays on metal surfaces and product produced thereby |
| JP4860085B2 (ja) * | 2000-03-16 | 2012-01-25 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 表面結合リガンドから溶出された分析物を捕捉するための方法 |
-
2001
- 2001-03-14 JP JP2001568086A patent/JP4860085B2/ja not_active Expired - Lifetime
- 2001-03-14 DE DE60121923T patent/DE60121923T2/de not_active Expired - Lifetime
- 2001-03-14 AT AT01915984T patent/ATE335202T1/de not_active IP Right Cessation
- 2001-03-14 EP EP01915984A patent/EP1264179B1/en not_active Expired - Lifetime
- 2001-03-14 WO PCT/SE2001/000531 patent/WO2001069258A1/en not_active Ceased
- 2001-03-14 AU AU2001242928A patent/AU2001242928A1/en not_active Abandoned
- 2001-03-16 US US09/810,937 patent/US6503760B2/en not_active Expired - Fee Related
-
2002
- 2002-11-15 US US10/295,709 patent/US6844201B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20020012929A1 (en) | 2002-01-31 |
| AU2001242928A1 (en) | 2001-09-24 |
| US20030077842A1 (en) | 2003-04-24 |
| DE60121923D1 (de) | 2006-09-14 |
| EP1264179B1 (en) | 2006-08-02 |
| ATE335202T1 (de) | 2006-08-15 |
| US6503760B2 (en) | 2003-01-07 |
| EP1264179A1 (en) | 2002-12-11 |
| JP2003527606A (ja) | 2003-09-16 |
| US6844201B2 (en) | 2005-01-18 |
| WO2001069258A1 (en) | 2001-09-20 |
| JP4860085B2 (ja) | 2012-01-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60121923T2 (de) | Verfahren zur erfassung von analyten, die aus oberflächengebundenen liganden eluiert werden | |
| DE69622176T2 (de) | Überwachung des refraktionsindex von optischen sensoren zur bestimmung von änderungen in der oberflächenstruktur | |
| DE602005005485T2 (de) | Assayvorrichtung und verfahren mit gesteuertem fluss | |
| DE69731306T2 (de) | Verfahren und kit zur bestimmung eines analyts mittels massenbestimmung | |
| DE60027578T2 (de) | Verfahren zum Nachweis von Bioanalyten unter Verwendung metallischer Nanohüllen | |
| DE602004013468T2 (de) | Nachweis von biomolekülen unter verwendung poröser biosensoren und raman-spektroskopie | |
| DE69228325T2 (de) | Ligandentest unter verwendung von interferenzmodulation | |
| DE69302273T2 (de) | Verfahren zur verbesserung der messgenauigkeit in messverfahren, die evanescent-wave-biosensoren verwenden | |
| JP2003527606A5 (https=) | ||
| DE60029528T2 (de) | Trennungsverfahren von Substanzen durch Dielektrophoresis | |
| DE69333050T2 (de) | Anordnung zur probenbestimmung | |
| DE69804612T2 (de) | Verfahren zur charakterisierung von proben unter verwendung statistischer zwischendaten | |
| DE60034029T2 (de) | Verfahren zur herstellung von festphasen-matrizen für ramanspektroskopie unter verstärkender oberflächenmitwirkung (sers) | |
| DE3786388T2 (de) | Analytisches Gerät und Verfahren zum Nachweis von Chlamydia Trachomatis und Neisseria Gonorrhoeae. | |
| EP1556695A2 (de) | Analytische plattform und nachweisverfahren mit gegebenenfalls nach fraktionierung in einer probe nachzuweisenden analyten als immobilisierten spezifischen bindungspartnern | |
| DE60028976T2 (de) | Analyseverfahren und vorrichtung | |
| EP1082601B1 (de) | Durchfluss-scheranalysator für biologisch aktive moleküle in flüssigkeitsschichten auf oberflächen, verfahren zur analyse einer flüssigkeit und verfahren zur bestimmung der dicke einer ultradünnen flüssigkeitsschicht | |
| WO1993019368A1 (de) | Einwegreaktionsgefäss für die festphasenimmunanalytik und verfahren zur messung von über immunreaktionen bestimmbaren komponenten | |
| DE102018209516A1 (de) | Verfahren zur Bestimmung von Analyten mittels kompetitiver Bindungsreaktion | |
| DE69803209T2 (de) | Ein verfahren zur probencharakterisierung durch bestimmung einer funktion mindestens einer spezifischen eigenschaft der probe | |
| DE3787706T2 (de) | Verfahren zur quantitativen Bestimmung der Antigene und Antikörper. | |
| WO2003079011A2 (de) | Verfahren zur quantitativen bestimmung mehrerer analyten | |
| DE69818706T2 (de) | Analytisches verfahren mit teilchen und testkit um das verfahren durchzuführen | |
| DE19943704C1 (de) | Affinitätssensor zum Nachweis biologischer und/oder chemischer Spezies und dessen Verwendung | |
| DE102004007005A1 (de) | Massenspektrometrische Häufigkeitsbestimmungen von Proteinen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: GE HEALTHCARE BIO-SCIENCES AB, UPPSALA, SE |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: HAMMONDS LLP, LONDON, GB |
|
| R082 | Change of representative |
Ref document number: 1264179 Country of ref document: EP Representative=s name: J D REYNOLDS & CO., GB |