DE4104782B4 - Novel plasmids containing DNA sequences that cause changes in carbohydrate concentration and carbohydrate composition in plants, as well as plants and plant cells containing these plasmids - Google Patents

Novel plasmids containing DNA sequences that cause changes in carbohydrate concentration and carbohydrate composition in plants, as well as plants and plant cells containing these plasmids Download PDF

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DE4104782B4
DE4104782B4 DE4104782A DE4104782A DE4104782B4 DE 4104782 B4 DE4104782 B4 DE 4104782B4 DE 4104782 A DE4104782 A DE 4104782A DE 4104782 A DE4104782 A DE 4104782A DE 4104782 B4 DE4104782 B4 DE 4104782B4
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fragment
plants
plasmid
plasmids
dna
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DE4104782A1 (en
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Lothar Prof. Dr. Willmitzer
Uwe Dr. Sonnewald
Jens Kossmann
Bernd Dr. Müller-Röber
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Bayer CropScience AG
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Bayer CropScience AG
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Priority to DE69233227T priority patent/DE69233227T2/en
Priority to US08/104,158 priority patent/US6215042B1/en
Priority to HU9302336A priority patent/HU217412B/en
Priority to CA002104123A priority patent/CA2104123C/en
Priority to DK92904007T priority patent/DK0571427T3/en
Priority to AU12265/92A priority patent/AU663072B2/en
Priority to PCT/EP1992/000302 priority patent/WO1992014827A1/en
Priority to JP4503977A priority patent/JPH07503122A/en
Priority to EP01100504A priority patent/EP1103617A3/en
Priority to EP92904007A priority patent/EP0571427B1/en
Priority to ES92904007T priority patent/ES2204889T3/en
Priority to AT92904007T priority patent/ATE251671T1/en
Priority to IE045092A priority patent/IE920450A1/en
Priority to IL100929A priority patent/IL100929A0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

Plasmids are described having DNA sequences that after insertion into the genome of the plants cause changes in the carbohydrate concentration and the carbohydrate composition in regenerated plants. These changes can be obtained from a sequence of a branching enzyme that is located on these plasmids. This branching enzyme alters the amylose/amylopectin ratio in starch of the plants, especially in commercially used plants.

Description

Die vorliegende Erfindung betrifft neue Plasmide, enthaltend eine DNA-Sequenz, dessen in dieser Sequenz enthaltenen Informationen nach Einführung in ein pflanzliches Genom Veränderungen der Karbohydratkonzentration und der Karbohydratzusammensetzung in regenerierten Pflanzen hervorrufen sowie Pflanzen und Pflanzenzellen enthaltend diese Plasmide. Die DNA-Sequenz ist die kodierende Sequenz eines Branching-Enzyms aus Kartoffelknollen. Bedingt durch den kontinuierlich steigenden Nahrungsmittel- und Rohstoffbedarf, der aus der ständig wachsenden Weltbevölkerung resultiert, ist eine der Aufgaben der biotechnischen Forschung, sich um eine Veränderung der Inhaltsstoffe sowie des Ertrages von Nutzpflanzen zu bemühen. Dazu muß der Metabolismus der Pflanzen verändert werden.The The present invention relates to novel plasmids containing a DNA sequence, the information contained in this sequence after introduction into a plant genome changes the carbohydrate concentration and the carbohydrates composition in regenerated plants as well as plants and plant cells containing these plasmids. The DNA sequence is the coding sequence a Branching enzyme from potato tubers. Due to the continuous rising food and commodity demand, which is out of the ever growing world population is one of the tasks of biotechnology research, a change the ingredients and crops of crops. To must the Metabolism of plants changed become.

Ein besonderes Interesse gilt dabei der Möglichkeit, pflanzliche Inhaltsstoffe als erneuerbare Rohstoffquellen für z.B. die chemische Industrie zu verwenden. Dies ist aus zwei Gründen von besonders großer Bedeutung. Zum einen stellen Erdöl- oder Kohlevorkommen die bisher von der petrochemischen Industrie im wesentlichen verwendeten Rohstoffquellen dar. Diese Vorräte aber sind endlich und es ist absehbar, daß alternative, erneuerbare Rohstoffquellen, d.h. nachwachsende Rohstoffe geschaffen werden müssen. Zum anderen ist die gegenwärtige Situation in der Landwirtschaft die, daß im europäischen sowie nordamerikanischen Raum die klassischen, auf den Ernährungssektor zielenden landwirtschaftlichen Produkte im Überschuß vorliegen. Dies führt zu offensichtlichen finanziellen und politischen Problemen in der Landwirtschaft. Alternative Produkte, für die ein großer mengenmäßiger Bedarf besteht, könnten eine Lösung dieses Problems darstellen.One special interest is the possibility of herbal ingredients as renewable sources of raw material for e.g. the chemical industry to use. This is particularly important for two reasons. Firstly, petroleum or coal deposits previously used by the petrochemical industry essentially used raw material sources. But these supplies are finite and it is foreseeable that alternative, renewable Raw material sources, i. renewable raw materials are created have to. The other is the present one Situation in agriculture that in the European as well as North American area the classic, the food sector target agricultural products in excess. This leads to obvious ones financial and political problems in agriculture. Alternative products, for the a large quantitative requirements exists, could a solution pose this problem.

Nachwachsende Rohstoffe lassen sich prinzipiell in Fette und Öle, Proteine sowie Carbohydratverbindungen wie Mono- und Polysaccharide unterteilen. Bei den Polysacchariden sind die Stärke und die Cellulose die mit Abstand wichtigsten Komponenten. Im Bereich der EG verteilte sich die Gesamtstärkeerzeugung des Jahres 1987/1988 auf die Pflanzen Mais (60 %), Weizen (19 %) und Kartoffel (21 %).renewable Raw materials can be found in fats and oils, proteins and carbohydrate compounds such as mono- and polysaccharides subdivide. For the polysaccharides are the strength and cellulose by far the most important components. In the area The EC distributed the total starch production of the year 1987/1988 on the plants corn (60%), wheat (19%) and potato (21%).

Für eine gesteigerte Nutzung pflanzlicher Stärke als industrieller Rohstoff sollte die Qualität der Stärke den Anforderungen der verarbeitenden Industrie stärker entgegenkommen. Hier sind im wesentlichen das Amylose zu Amylopektin Verhältnis, die Kettenlänge, der Verzweigungsgrad beim Amylopektin sowie die Größe der Stärkekörner zu nennen.For an increased Use of vegetable starch As an industrial raw material, the quality of the starch should meet the requirements of the processing industry Industry stronger accommodated. Here are essentially the amylose to amylopectin Relationship, the chain length, the degree of branching in amylopectin and the size of the starch granules too call.

Die wesentlichen biochemischen Synthesewege zum Aufbau der Stärke in höheren Pflanzen sind bekannt. Stärke bestehend aus Amylose und Amylopektin, wobei Amylose aus linearen α-1,4-Glukanen besteht und Amylopektin aus α-1,4-Glucanen besteht, die mit einer variierenden Frequenz über α-1,6-Bindungen miteinander verbunden sind und somit zu einem verzweigten Polyglukan führen. Für die Einführung der α-1,6-Verknüpfungen ist das sogenannte Verzweigungsenzym (Branching Enzym oder Q-Enzym) verantwortlich. Ein Weg zur Erzeugung von Stärke, die lediglich aus linearen α-1,4-Glukanen aufgebaut ist, ist von daher die Inhibierung der enzymatischen Aktivität dieses Proteins bzw. die Inhibierung der Biosynthese des Verzweigungsenzyms. Neue biotechnologische Verfahren zur genetischen Veränderung dikotyler und monokotyler Pflanzen mittels des Transfers und stabilen Einbaus einzelner isolierter Gene oder Gruppen von Genen sind bekannt (Gasser und Fraley, Science 244, 1293-1299). Möglichkeiten zur spezifischen Expression von mittels gentechnologischen Verfahren in die Pflanze eingeführten fremden Genen, primär in der Kartoffelknolle sind ebenfalls bekannt ( EP 375092 und Rocha-Sosa et al., EMBO J. 8, 23-29 (1989)).The essential biochemical pathways for building starch in higher plants are known. Starch consisting of amylose and amylopectin, wherein amylose consists of linear α-1,4-glucans and amylopectin consists of α-1,4-glucans which are linked together at a varying frequency via α-1,6 bonds and thus to a branched polyglucan lead. The so-called branching enzyme (branching enzyme or Q-enzyme) is responsible for the introduction of α-1,6 linkages. One way of producing starch composed solely of linear α-1,4-glucans is therefore to inhibit the enzymatic activity of this protein or inhibit the biosynthesis of the branching enzyme. New biotechnological methods for the genetic modification of dicotyledonous and monocotyledonous plants by means of the transfer and stable incorporation of individual isolated genes or groups of genes are known (Gasser and Fraley, Science 244, 1293-1299). Possibilities for the specific expression of foreign genes introduced into the plant by means of genetic engineering, primarily in the potato tuber, are also known ( EP 375092 and Rocha-Sosa et al., EMBO J. 8, 23-29 (1989)).

In EP 455316 wird die Herstellung von transgenen Pflanzen beschrieben, die eine veränderte Aktivität von ADP-Glucose-Pyrophosphorylase (ADPase) bzw. eines Patatin-Proteins aufweisen. Es wird ausgesagt, dass die Reduzierung der ADPase-Aktivität zu einer Verringerung der Stärkekonzentration und gleichzeitig zu einer Erhöhung der Mono- und Oligosaccharidkonzentration führt.In EP 455316 describes the production of transgenic plants which have an altered activity of ADP-glucose pyrophosphorylase (ADPase) or of a patatin protein. It is stated that the reduction of the ADPase activity leads to a reduction in the starch concentration and at the same time to an increase in the mono- and oligosaccharide concentration.

Bhattacharyya et al. (Cell 60 (1990), 115-122) beschreibt die Klonierung von DNA-Sequenzen aus Erbse, die ein Branching-Enzym codieren.Bhattacharyya et al. (Cell 60 (1990), 115-122) describes the cloning of DNA sequences from pea encoding a branching enzyme.

Aufgabe der vorliegenden Erfindung ist es, Plasmide bereitzustellen, deren DNA-Sequenzen nach Einführung in ein pflanzliches Genom Veränderungen der Karbohydratkonzentration und der Karbohydratzusammensetzung in regenerierten Pflanzen hervorrufen. Es ist ferner Aufgabe der vorliegenden Erfindung, Pflanzen und Pflanzenzellen aus denen ganze Pflanzen regeneriert werden, die diese Plasmide enthalten, bereitzustellen.The object of the present invention is to provide plasmids whose DNA sequences after introduction into a plant genome cause changes in the carbohydrate concentration and the carbohydrate composition in regenerated plants. It is a further object of the present invention, plants and plant cells from which whole plants are regenerated, containing these plasmids, be Semi note.

Es wurde nun gefunden, daß Plasmide, enthaltend eine neue DNA-Sequenz, dessen in dieser Sequenz enthaltenen Informationen nach Einführung in. das pflanzliche Genom zu einer Veränderung der Karbohydratkonzentration und der Karbohydratzusammensetzung in Pflanzen führen.It it has now been found that plasmids, containing a new DNA sequence whose sequence contained in this sequence Information after introduction in. the plant genome to a change in carbohydrate concentration and the carbohydrates composition in plants.

Bei der DNA-Sequenz handelt es sich um eine kodierende Sequenz eines Branching-Enzyms aus Kartoffelknollen. Bei den Karbohydraten handelt es sich um Mono-, Di-, Oligo- oder Polysaccharide, wobei unter Poly saccharid auch Stärke zu verstehen ist. Bei der Stärke handelt es sich um die Komponenten Amylose und Amylopektin. Das Amylose/Amylopektin-Verhältnis kann durch ein Branching-Enzym der Sequenz

Figure 00040001
Figure 00050001
die sich auf den erfindungsgemäßen Plasmiden befindet, als auch durch Plasmide, die eine leicht veränderte Nukleotid-Sequenz enthalten, in Pflanzen oder Pflanzenzellen verändert werden. Pflanzenzellen, die diese Plasmide enthalten, können zu vollständigen transgenen Pflanzen regeneriert werden. Um wirksam zu sein, ist die DNA-Sequenz des Branching-Enzyms an die regulatorischen Sequenzen anderer Gene, die eine Transkription der das Branching-Enzym kodierenden DNA-Sequenz sicherstellen, fusioniert. Die DNA-Sequenz kann auch in invertierter Anordnung an die regulatorischen Sequenzen anderer Gene fusioniert sein, wobei das 3'-Ende der kodierenden Sequenz an das 3'-Ende des Promotors und das 5'-Ende des Terminationssignals fusioniert ist. Hierbei wird eine antisense RNA in der Pflanze vom Branching-Enzym gebildet. Die regulatorischen Sequenzen sind hierbei Promotoren und Terminationssignale von pflanzlichen oder viralen Genen, wie z.B. der Promotor der 35S RNA des Blumenkohl-Mosaik-Virus oder der Promotor des Klasse I Patatin-Gens B 33 und das Terminationssignal des 3'-Endes des Octopin-Synthase-Gens der T-DNA des Ti-Plasmids pTiACH5. Die in den erfindungsgemäßen Plasmiden enthaltene DNA kann zur Herstellung transgener Nutzpflanzen, wie Mais, Gerste, Weizen, Reis, Erbse, Sojabohne, Zuckerrohr, Zuckerrübe, Tomate, Kartoffel oder Tabak verwendet werden.The DNA sequence is a coding sequence of a Branching enzyme from potato tubers. The carbohydrates are mono-, di-, oligo- or polysaccharides, where poly-saccharide is also starch. The starch is the components amylose and amylopectin. The amylose / amylopectin ratio can be determined by a branching enzyme of the sequence
Figure 00040001
Figure 00050001
which is located on the plasmids according to the invention, as well as by plasmids containing a slightly altered nucleotide sequence are changed in plants or plant cells. Plant cells containing these plasmids can be regenerated to complete transgenic plants. To be effective, the DNA sequence of the Branching enzyme is fused to the regulatory sequences of other genes ensuring transcription of the DNA sequence encoding the Branching enzyme. The DNA sequence may also be fused in an inverted arrangement to the regulatory sequences of other genes, with the 3 'end of the coding sequence fused to the 3' end of the promoter and the 5 'end of the termination signal. Here, an antisense RNA is formed in the plant by Branching enzyme. The regulatory sequences here are promoters and termination signals of plant or viral genes, such as the promoter of the 35S RNA of the cauliflower mosaic virus or the promoter of the class I patatin gene B 33 and the termination signal of the 3'-end of octopine synthase Gene of the T-DNA of the Ti plasmid pTiACH5. The DNA contained in the plasmids of the invention can be used for the production of transgenic crops such as corn, barley, wheat, rice, pea, soybean, sugar cane, sugar beet, tomato, potato or tobacco.

Begriffe und AbkürzungenTerms and Abbreviations

AbkürzungenAbbreviations

  • bp, Kbbp, Kb
    = Basenpaare, Kilobasen= Base pairs, kilobases
    DNADNA
    = Desoxyribonukleinsäure, Träger der genetischen Information= Deoxyribonucleic acid, carrier of genetic information
    RNARNA
    = Ribonukleinsäure= Ribonucleic acid
    SDSSDS
    = Natriumdodecylsulfat= Sodium dodecyl sulfate
    TrisTris
    = Tris(2-aminoäthyl)-amin= Tris (2-aminoethyl) amine

Begriffe Elektrophorese = biochemisches Trennverfahren zur Trennung von Proteinen und Nukleinsäuren nach Größe und Ladung. Expression = Aktivität eines Gens Gen = Erbfaktor; eine Einheit des Erbguts, Träger der Teilinformation für ein bestimmtes Merkmal. Gene bestehen aus Nukleinsäuren (z.B. DNA, RNA). Genom = Gesamtzahl der auf den Chromosomen der Zelle lokalisierten Gene. Klon = Zellpopulation, die von einer einzigen Mutterzelle stammt. Die Nachkommen sind genotypisch gleich. Durch Klonierung kann die Einheitlichkeit von Zellinien noch gesteigert werden. terms electrophoresis = biochemical separation method for the separation of proteins and nucleic acids by size and charge. expression = Activity of a gene gene = Genetic factor; a unit of genetic material, bearer of partial information for a given characteristic. Genes consist of nucleic acids (eg DNA, RNA). genome = Total number of genes located on the chromosomes of the cell. clone = Cell population derived from a single parent cell. The offspring are genotypically the same. By cloning the uniformity of cell lines can be increased.

Linker, Polylinkerleft, polylinker = synthetische DNA-Sequenz, die eine oder mehrere (Polylinker) Restriktionsschnittstellen in unmittelbarer Reihenfolge enthält.= synthetic DNA sequence containing one or more (polylinker) restriction sites contains in immediate order. Northern Blot, Southern BlotNorthern Blot, Southern blot = Transfer und Fixierung elektrophoretisch aufgetrennter RNA oder DNA auf eine Nitrozellulose- oder Nylonmembran= Transfer and fixation of electrophoretically separated RNA or DNA on a nitrocellulose or nylon membrane Plasmidplasmid = zusätzlicher, extrachromosomaler DNA-Erbträger in Bakterienzellen (evtl. auch in Eukaryonten), der sich unabhängig vom Bakterienchromosom redupliziert. Das Plasmid kann in andere Wirts-DNA integriert werden.= additional, extrachromosomal DNA genetic carrier in bacterial cells (possibly also in eukaryotes), which is independent of Bacterial chromosome reduplicated. The plasmid can be transformed into other host DNA to get integrated. Promotorpromoter = Kontrollsequenz der DNA-Expression, die die Transkription homologer oder heterologer DNA-Gen-Sequenzen gewährleistet.= Control sequence of DNA expression, the transcription homologous or heterologous DNA gene sequences. Restriktionsenzymerestriction enzymes = Restriktionsendonukleasen, die eine Untergruppe der Endodesoxyribonukleasen sind (z.B. EcoRI (Spezifität G↓AATTC und EcoRII↓CC ( A) / T A) GG, zeichnen sich durch eine hohe Spezifität der Substraterkennung aus (↓ = Spaltstelle).= Restriction endonucleases, which are a subgroup of endodeoxyribonucleases (eg EcoRI (specificity G ↓ AATTC and EcoRII ↓ CC (A) / TA) GG, are characterized by a high specificity of substrate recognition ( ↓ = cleavage site). Restriktionsstellenrestriction sites = Spaltstellen, die spezifisch durch Restriktionsenzyme erzeugt werden.= Cleavage sites specifically generated by restriction enzymes. Transformation transformation = Einfügung exogener DNA eines Bakterienstammes in eine Empfängerzelle.= insertion exogenous DNA of a bacterial strain into a recipient cell. Vektoren vectors = wirtsspezifische, replizierfähige Strukturen, die Gene aufnehmen und diese auf andere Zellen übertragen. Plasmide können als Vektoren benutzt werden.= host-specific, replicable Structures that pick up genes and transfer them to other cells. Plasmids can to be used as vectors.

Am 20.08.1990 wurden bei der Deutschen Sammlung von Mikroorganismen (DSM) in Braunschweig, Bundesrepublik Deutschland, folgende Plasmide hinterlegt (Hinterlegungsnummer): Plasmid P35 S-BE (DSM 6143) Plasmid P35 S-anti-BE (DSM 6144) Plasmid P33-BE (DSM 6145) Plasmid P33-anti-BE (DSM 6146) On 20.08.1990, the following plasmids were deposited with the German Collection of Microorganisms (DSM) in Braunschweig, Federal Republic of Germany (accession number): Plasmid P35 S-BE (DSM 6143) Plasmid P35 S-anti-BE (DSM 6144) Plasmid P33-BE (DSM 6145) Plasmid P33 anti-BE (DSM 6146)

Beschreibung der Abbildungendescription of the pictures

1 zeigt die Struktur des 13,6 kb großen Plasmids P35 S-BE. Das Plasmid enthält folgende Fragmente:

  • A = Fragment A (529 bp) beinhaltet den 35S Promotor des Blumenkohl-Mosaik-Virus (CaMV). Das Fragment umfaßt die Nukleotide 6909 bis 7437 des CaMV.
  • B = Fragment B (2909 bp) beinhaltet das DNA-Fragment, welches für das Branching-Enzym kodiert.
  • C = Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5 der Nukleotide 11749-11939.
1 shows the structure of the 13.6 kb plasmid P35 S-BE. The plasmid contains the following fragments:
  • A = fragment A (529 bp) contains the cauliflower mosaic virus (CaMV) 35S promoter. The fragment comprises nucleotides 6909 to 7437 of CaMV.
  • B = fragment B (2909 bp) contains the DNA fragment which codes for the branching enzyme.
  • C = fragment C (192 bp) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5 of nucleotides 11749-11939.

Es werden ferner die im Ausführungsbeispiel 1 beschriebenen Schnittstellen gezeigt.It are also in the embodiment 1 described interfaces.

2 zeigt die Struktur des 13,6 kb großen Plasmids P35 S-anti-BE. Das Plasmid enthält folgende Fragmente:

  • A = Fragment A (529 bp) beinhaltet den 35S Promotor des Blumenkohl-Mosaik-Virus (CaMV). Das Fragment umfaßt die Nukleotide 6909 bis 7437 des CaMV.
  • B = Fragment B (2909 bp) beinhaltet das DNA-Fragment, welches für das Verzweigungsenzym kodiert.
  • C = Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5. Das Fragment umfaßt die Nukleotide 11749-11939.
2 shows the structure of the 13.6 kb plasmid P35 S-anti-BE. The plasmid contains the following fragments:
  • A = fragment A (529 bp) contains the cauliflower mosaic virus (CaMV) 35S promoter. The fragment comprises nucleotides 6909 to 7437 of CaMV.
  • B = fragment B (2909 bp) contains the DNA fragment coding for the branching enzyme.
  • C = fragment C (192 bp) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5. The fragment comprises nucleotides 11749-11939.

Es werden ferner die im Ausführungsbeispiel 2 beschriebenen Schnittstellen gezeigt.It are also in the embodiment 2 described interfaces.

3 zeigt die Struktur des 14,6 kb großen Plasmids P33-BE. Das Plasmid enthält folgende Fragmente:

  • A = Fragment A (1526 bp) beinhaltet das DraI-DraI-Fragment der Promotorregion des Patatin-Gens B33. Das Fragment umfaßt die Nukleotidpositionen –1512 bis +14.
  • B = Fragment B (2909 bp) beinhaltet das DNA-Fragment, welches für das Verzweigungsenzym kodiert.
  • C = Fragment C (192 pb) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5. Das Fragment umfaßt die Nukleotidpositionen 11749-11939.
3 shows the structure of the 14.6 kb plasmid P33-BE. The plasmid contains the following fragments:
  • A = fragment A (1526 bp) contains the DraI-DraI fragment of the promoter region of the patatin gene B33. The fragment comprises nucleotide positions -1512 to +14.
  • B = fragment B (2909 bp) contains the DNA fragment coding for the branching enzyme.
  • C = fragment C (192 pb) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5. The fragment comprises nucleotide positions 11749-11939.

Es werden ferner die im Ausführungsbeispiel 3 beschriebenen Schnittstellen gezeigt.It are also in the embodiment 3 described interfaces shown.

4 zeigt die Struktur des 14,6 großen Plasmids P33-anti-BE. Das Plasmid enthält folgende Fragmente:

  • A = Fragment A (1526 bp) beinhaltet das Dra-I-DraI-Fragment der Promotorregion des Patatin Gens B 33. Das Fragment umfaßt die Nukleotidpositionen –1512 bis +14.
  • B = Fragment B (2909 bp) beinhaltet das cDNA-Fragment, das für das Verzweigungsenzym kodiert.
  • C = Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5. Das Fragment umfaßt die Nukleotide 11749-11939.
4 shows the structure of the 14.6 large plasmid P33-anti-BE. The plasmid contains the following fragments:
  • A = fragment A (1526 bp) contains the Dra I-DraI fragment of the promoter region of the patatin gene B 33. The fragment comprises nucleotide positions -1512 to +14.
  • B = fragment B (2909 bp) contains the cDNA fragment encoding the branching enzyme.
  • C = fragment C (192 bp) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5. The fragment comprises nucleotides 11749-11939.

Es werden ferner die im Ausführungsbeispiel 4 beschriebenen Schnittstellen gezeigt.It are also in the embodiment 4 described interfaces shown.

Zum besseren Verständnis der dieser Erfindung zugrundeliegenden Ausführungsbeispiele wird vorab eine Aufstellung aller für diese Versuche notwendigen und an sich bekannten Verfahren gegeben:
Die Konzentration "mM" bezieht sich auf mMol/l.For a better understanding of the embodiments of this invention underlying a list of all necessary for these experiments and known per se methods is given:
The concentration "mM" refers to mmol / l.

1. Klonierungsverfahren1. Cloning method

Zur Klonierung wurden die Vektoren pUC18/19 und pUC118 sowie die M13 mP10 Serien (Yanisch-Perron et. al., Gene (1985), 33, 103-119), benutzt.to Cloning was carried out using the vectors pUC18 / 19 and pUC118 as well as the M13 mP10 series (Yanisch-Perron et al., Gene (1985), 33, 103-119), used.

Für die Pflanzentransformation wurden die Genkonstruktionen in den binären Vektor BIN19 (Bevan, Nucl. Acids Res. (1984), 12, 8711-8720) kloniert.For the plant transformation The gene constructs were transformed into the binary vector BIN19 (Bevan, Nucl. Acids Res. (1984), 12, 8711-8720).

2. Bakterienstämme2. Bacterial strains

Für die pUC- und M13 mP-Vektoren wurden die E. coli-Stämme BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977). 24, 6342-6346) oder TB1 benutzt.For the pUC and M13 mP vectors were the E. coli strains BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977). 24, 6342-6346) or TB1.

Für den Vektor BIN19 wurde ausschließlich der E. coli-Stamm TB1 benutzt. TB1 ist ein Rekombinations-negatives, Tetracyclin-resistentes Derivat des Stammes JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). Der Genotyp des TB1-Stammes ist (Bart Barrel, persönliche Mitteilung): F'(traD36, proAB, lacI, lacZΔM15), Δ(lac, pro), SupE, thiS, recA, Sr1::Tn10(TcR).For the vector BIN19 became exclusive the E. coli strain TB1 used. TB1 is a recombination-negative, Tetracycline-resistant derivative of strain JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). The genotype of the TB1 strain is (Bart Barrel, personal Message): F '(traD36, proAB, lacI, lacZΔM15), Δ (lac, pro), SupE, thiS, recA, Sr1 :: Tn10 (TcR).

Die Transformation der Plasmide in die Kartoffelpflanze wurde mit Hilfe des Agrobacterium tumefaciens-Stammes LBA4404 (Bevan, M., Nucl. Acids Res. 12, 8711-8721, (1984); BIN19 Derivat) durchgeführt.The Transformation of the plasmids into the potato plant was using of Agrobacterium tumefaciens strain LBA4404 (Bevan, M., Nucl. Acids Res. 12, 8711-8721, (1984); BIN19 derivative).

3. Transformation von Agrobacterium tumefaciens3. Transformation of Agrobacterium tumefaciens

Bei Bin19-Derivaten erfolgt die Einführung der DNA in die Agrobakterien durch direkte Transformation nach der Methode von Holsters et al., (Mol. Gen. Genet. (1978), 163, 181-187). Die Plasmid-DNA transformierter Agrobakterien wurde nach der Methode von Birnboim und Doly (Nucl. Acids Res. (1979), 7, 1513-1523) isoliert und nach geeigneter Restriktionsspaltung gelelektrophoretisch aufgetrennt.at Bin19 derivatives are introduced the DNA into the agrobacteria by direct transformation after the Method of Holsters et al., (Mol. Gen. Genet. (1978), 163, 181-187). The plasmid DNA of transformed Agrobacteria was determined by the method Birnboim and Doly (Nucl. Acids Res. (1979), 7, 1513-1523) and separated by gel electrophoresis after suitable restriction cleavage.

4. Pflanzentransformation4. Plant transformation

10 kleine, mit einem Skalpell verwundete Blätter einer Kartoffel-Sterilkultur wurden in 10 ml MS-Medium mit 2 % Saccharose gelegt, welches 30 – 50 μl einer unter Selektion gewachsenen Agrobacterium tumefaciens-Übernachtkultur enthielt. Nach 3-5 minütigem, leichtem Schütteln wurden die Petrischalen bei 25°C im Dunkeln inkubiert. Nach 2 Tagen wurden die Blätter auf MS-Medium mit 1,6 % Glukose, 2 mg/l Zeatinribose, 0,02 mg/l Naphthylessigsäure, 0,02 mg/l Giberellinsäure, 500 mg/l Claforan, 50 mg/l Kanamycin und 0,8 % Bacto-Agar ausgelegt. Nach einwöchiger Inkubation bei 25°C und 3000 Lux wurde die Claforankonzentration im Medium um die Hälfte reduziert. Die weitere Regeneration und Kultivierung der Pflanzen erfolgte nach literaturbekannten Verfahren (Rocha-Sosa et al., EMBO Journal 8, 23-29 (1989).10 small, wounded with a scalpel leaves of a potato sterile culture were placed in 10 ml MS medium with 2% sucrose, which 30 - 50 ul of a lower Selection grown Agrobacterium tumefaciens overnight culture contained. To 3-5 minutes, gentle shaking the Petri dishes were at 25 ° C incubated in the dark. After 2 days the leaves became MS medium at 1.6 % Glucose, 2 mg / l zeatinribose, 0.02 mg / l naphthylacetic acid, 0.02 mg / l gibberellin acid, 500 mg / L claforan, 50 mg / L kanamycin and 0.8% Bacto agar. After a week Incubation at 25 ° C and 3000 lux, the claforan concentration in the medium was reduced by half. The further regeneration and cultivation of the plants took place according to literature methods (Rocha-Sosa et al., EMBO Journal 8, 23-29 (1989).

5. Analyse genomischer DNA aus transgenen Kartoffelpflanzen5. Analysis of genomic DNA from transgenic potato plants

Die Isolierung genomischer Pflanzen-DNA erfolgte nach Rogers und Bendich (Plant Mol. Biol. (1985), 5, 69-76.The Isolation of genomic plant DNA was carried out according to Rogers and Bendich (Plant Mol. Biol. (1985), 5, 69-76.

Für die DNA-Analyse wurden 10 – 20 μg DNA nach geeigneter Restriktionsspaltung mit Hilfe von "Southern Blots" auf Integration der zu untersuchenden DNA-Sequenzen analysiert.For DNA analysis 10-20 μg of DNA were recovered appropriate restriction cleavage with the help of "Southern blots" for integration of the investigated Analyzed DNA sequences.

6. Analyse der Gesamt-RNA aus transgenen Kartoffelpflanzen6. Analysis of Total RNA from transgenic potato plants

Die Isolierung pflanzlicher Gesamt-RNA erfolgte nach Logemann et al. (Analytical Biochem. (1987), 163, 16-20).The Isolation of total plant RNA was carried out according to Logemann et al. (Analytical Biochem. (1987), 163, 16-20).

Für die Analyse wurden je 50 μg Gesamt-RNA mit Hilfe von "Northern Blots" auf die Anwesenheit der gesuchten Transkripte untersucht.For the analysis were each 50 ug Total RNA with the help of "Northern Blots "on the presence the searched transcripts.

7. Proteinextraktion7. Protein extraction

Zur Extraktion von Gesamtprotein aus Pflanzengewebe wurden Gewebestücke in Proteinextraktionspuffer (25 mM Natriumphosphat pH 7,0, 2 mM Natriumbisulfit, unter Zusatz von 0,1 % (w/v) unlöslichem Polyvinylpolypyrrolidon (PVP) homogenisiert.to Extraction of total protein from plant tissue were pieces of tissue in protein extraction buffer (25 mM sodium phosphate pH 7.0, 2 mM sodium bisulfite, with addition of 0.1% (w / v) insoluble Polyvinylpolypyrrolidone (PVP) homogenized.

Nach Filtrieren durch Zellstoff wurden Zelltrümmer für 20 Minuten bei 10000 Umdrehungen pro Minute abzentrifugiert und die Proteinkonzentration des Überstandes nach der Methode von Bradford (Anal. Biochem. (1976)/ 72, 248-254) bestimmt.To Filtering through pulp was cell debris for 20 minutes at 10,000 revolutions Centrifuged per minute and the protein concentration of the supernatant according to the method of Bradford (Anal. Biochem. (1976) / 72, 248-254) certainly.

8. Nachweis von Fremdproteinen mit Hilfe immunologischer Verfahren (Western-Blot)8. Detection of foreign proteins using immunological methods (Western Blot)

Die Proteinextrakte wurden mittels Gelelektrophorese in SDS-PAGE (Natriumdodecylsulfat-Polyacrylamid) Gelen nach Molekulargewicht getrennt. Nach SDS-PAGE wurden Proteingele für 15-30 Minuten in Transfer Puffer für Graphitelektroden (48 g/l Tris, 39 g/l Glycin, 0,0375 % SDS, 20 % Methanol) äquilibriert und anschließend im Kühlraum auf Nitrozellulose-Filter transferiert und mit 1,3 mA/cm2 für 1-2 Stunden getrennt. Der Filter wurde für 30 Minuten mit 3 % Gelatine in TBS-Puffer (20 mM Tris/HCl pH 7,5, 500 mM NaCl) abgesättigt, anschließend wurde der Filter für 2 Stunden mit dem entsprechenden Antiserum in geeigneter Verdünnung (1:1000 – 10000 in TBS Puffer) bei Raumtemperatur inkubiert. Danach wurde der Filter jeweils für 15 Minuten mit TBS-, TTBS- (TBS-Puffer mit 0,1 % Polyoxyethylen-(20)-Sorbitanmonolaurat und TBS-Puffer gewaschen. Nach dem Waschen wurde der Filter für 1 Stunde bei Raumtemperatur mit Alkalische-Phosphatase konjugierten Goat-anti-Rabbit (GAR)-Antikörpern (1:7500 in TBS) inkubiert. Anschließend wurde der Filter wie obenstehend beschrieben gewaschen und in AP-Puffer (100 mM Tris/HCl pH 9,5, 100 mM NaCl, 5 mM MgCl2) äquilibriert. Die Alkalische-Phosphatase Reaktion wurde durch Substratzugabe von 70 μl 4-Nitrotetrazolium (NBT)-Lösung 150 mg/ml NBT in 70 % Dimethylformamid) und 35 μl 5-Brom-4-Chlor-3-Indolylphosphat (BCIP) (50 mg/ml BCIP in Dimethylformamid) in 50 ml AP-Puffer gestartet. Nach 5 Minuten konnten in der Regel erste Signale beobachtet The protein extracts were separated by molecular weight by gel electrophoresis in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide) gels. After SDS-PAGE, protein gels were equilibrated for 15-30 minutes in transfer buffer for graphite electrodes (48 g / l Tris, 39 g / l glycine, 0.0375% SDS, 20% methanol) and then transferred to nitrocellulose filters in the refrigerator and separated with 1.3 mA / cm 2 for 1-2 hours. The filter was saturated for 30 minutes with 3% gelatin in TBS buffer (20 mM Tris / HCl pH 7.5, 500 mM NaCl), then the filter was incubated for 2 hours with the appropriate antiserum in a suitable dilution (1: 1000). 10000 in TBS buffer) at room temperature. Thereafter, the filter was washed for 15 minutes each with TBS, TTBS (TBS buffer containing 0.1% polyoxyethylene (20) sorbitan monolaurate and TBS buffer.) After washing, the filter was incubated for 1 hour at room temperature with alkaline solution. Phosphatase-conjugated Goat anti-Rabbit (GAR) antibodies (1: 7500 in TBS) were then washed and washed in AP buffer (100 mM Tris / HCl pH 9.5, 100 mM NaCl, 5 mM MgCl 2) equilibrated. The alkaline phosphatase reaction was (by substrate addition of 70 ul 4 nitrotetrazolium (NBT) solution, 150 mg / ml NBT in 70% dimethylformamide) and 35 ul 5-bromo-4-chloro-3-indolyl phosphate BCIP) (50 mg / ml BCIP in dimethylformamide) in 50 ml of AP buffer. After 5 minutes usually first Signals observed

9. Bestimmung des Amylose/Amylopectin-Gehalts in der Stärke transgener Kartoffelpflanzen9. Determination of amylose / amylopectin content in strength transgenic potato plants

Blattstückchen mit einem Durchmesser von 10 mm wurden unter Dauerlicht auf 6 %iger Saccharoselösung 14 Stunden lang flotiert. Durch diese Lichtinkubation wurde eine stark erhöhte Stärkebildung in den Blattstückchen induziert. Nach der Inkubation wurde die Amylose- und Amylopectin-Konzentration nach Hovenkamp-Hermelink et al. (Potato Research 31, 241-246 (1988) bestimmt.Leaf pieces with a diameter of 10 mm were under continuous light to 6% sucrose solution Floats for 14 hours. This light incubation became one greatly increased strength training in the leaf pieces induced. After incubation, the amylose and amylopectin concentrations became according to Hovenkamp-Hermelink et al. (Potato Research 31, 241-246 (1988) certainly.

Die folgenden Ausführungsbeispiele erläutern die Herstellung der erfindungsgemäßen Plasmide, die Einführung dieser Plasmide in die Pflanze und die Funktion der Plasmide in diesen transgenen Pflanzen.The following embodiments explain the preparation of the plasmids according to the invention, the introduction of these Plasmids in the plant and the function of the plasmids in these transgenic plants.

Ausführungsbeispiel 1Embodiment 1

Herstellung des Plasmids P35s-BE und Einführung des Plasmids in das pflanzliche Genom der KartoffelPreparation of the plasmid P35s-BE and introduction of the plasmid into the vegetable genome of the potato

Aus einer in dem Expressionvektor gt11 angelegten cDNA Bank wurden verschiedene Klone identifiziert, die mit einem gegen das Verzweigungsenzym (Branching Enzym) aus Kartoffel gerichteten Antikörper kreuzreagieren. Diese Klone wurden benutzt, um aus einer in der HindII-Stelle des Vektors pUC 19 angelegten cDNA Bank Vollängenklone zu identifizieren, die aus isolierter mRNA von wachsenden Kartoffelknollen stammen. Einer der so isolierten Klone hatte eine Insertgröße von 2909 bp der Sequenz

Figure 00160001
Figure 00170001
From a cDNA library applied in the expression vector gt11, various clones were identified which cross-react with an antibody directed against potato branching enzyme. These clones were used to identify full-length clones derived from isolated mRNA from growing potato tubers from a cDNA library created in the HindII site of vector pUC19. One of the clones isolated in this way had an insert size of 2909 bp of the sequence
Figure 00160001
Figure 00170001

Für die weiteren Versuche wurde die in diesem Klon enthaltene 2909 bp lange c-DNA verwendet. Sie trägt die Bezeichnung cBE.For the others Experiments were the 2909 bp c-DNA contained in this clone used. she wears the name cBE.

Zur Herstellung des Plasmids p35s-BE wurde diese cDNA mit dem Promotor der 35s-RNA des Blumenkohl-Mosaik-Virus sowie dem Polyadenylierungssignal der Octopinsynthase des Ti-Plasmids pTiACH5 versehen. Dabei wurde die Orientierung der das Verzweigungsenzym kodierenden cDNA so gewählt, daß der kodierende Strang abgelesen wird (sense-Orientierung). Das Plasmid p35s-BE hat eine Größe von 13,6 kb und besteht aus den drei Fragmenten A, B und C, die in die Schnittstellen des Polylinkers von BIN19 kloniert wurden.to Preparation of the plasmid p35s-BE was this cDNA with the promoter the cauliflower mosaic virus 35s RNA and the polyadenylation signal the octopine synthase of the Ti plasmid pTiACH5 provided. It was the orientation of the cDNA coding the branching enzyme chosen so that the coding Strang is read (sense orientation). The plasmid p35s-BE has a size of 13.6 kb and consists of the three fragments A, B and C, which are in the interfaces of the polylinker of BIN19.

Fragment A (529 bp) beinhaltet den 35S Promotor des Blumenkohl-Mosaik-Virus (CAMV). Das Fragment umfaßt die Nukleotide 6909 bis 7437 des CaMV (Franck et al., Cell 21, 285-294). Es wurde als EcoRI-KpnI-Fragment aus dem Plasmid pDH51 (Pietrzak et al., Nucleic Acids Research 14, 5857-5868) isoliert und zwischen die EcoRI-KpnI-Schnittstellen des Polylinkers des Plasmids BIN 19 kloniert.Fragment A (529 bp) contains the cauliflower mosaic virus (CAMV) 35S promoter. The fragment comprises nucleotides 6909 to 7437 of CaMV (Franck et al., Cell 21, 285-294). It was called EcoRI-Kp nI fragment from the plasmid pDH51 (Pietrzak et al., Nucleic Acids Research 14, 5857-5868) and cloned between the EcoRI-KpnI sites of the polylinker of the plasmid BIN 19.

Fragment B enthält das 2909 bp große cDNA Fragment cBE, welches für das Branching-Enzym kodiert. Es wurde als HindIII-SmaI-Fragment aus dem Vektor pUC 19 herausgeschnitten und nach Auffüllen der Hind-III-Stelle mit DNA-Polymerase in die SmaI-Stelle des Polylinkers von BIN 19 kloniert. Dabei wurde die Orientierung der cDNA so gewählt, daß der kodierende Strang ab gelesen und damit eine sense-RNA gebildet wird. Die Schnittstellen BamHI/XbaI und PstI/SphI stammen aus dem Polylinker von pUC 19. Die Schnitt stellen BamHI/XbaI/SalI/PstI stammen aus dem Polylinker von BIN 19. Die auf dem Fragment B enthaltenen zwei EcoRI-Schnittstellen sind interne Schnitt stellen dieses Fragments.fragment B contains the 2909 bp big cDNA fragment cBE, which is for encodes the branching enzyme. It was called HindIII-SmaI fragment cut out of the vector pUC 19 and after filling the Hind III site with DNA polymerase into the SmaI site of the polylinker of BIN 19 cloned. It was the orientation of the cDNA chosen so that the read from coding strand and thus a sense RNA is formed. The interfaces BamHI / XbaI and PstI / SphI originate from the polylinker of pUC 19. The sections BamHI / XbaI / SalI / PstI are from the polylinker of BIN 19. The two EcoRI sites contained on fragment B are internal cut points of this fragment.

Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5 (Gelen et al., EMBO J. 3, 835,846), Nukleotide 11749-11939, welches als PvuII-HindIII-Fragment aus dem Plasmid pAGV 40 (Herrera-Estrella et al., (1983) Nature 303, 209-213) isoliert worden ist und nach Addition von SphI-linkern an die PvuII Schnittstelle zwischen die SphI-Hind-III-Schnittstellen des Polylinkers von BIN 19 kloniert worden war (s. 1).Fragment C (192 bp) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5 (Gelen et al., EMBO J. 3, 835, 846), nucleotides 11749-11939, which is a PvuII-HindIII fragment from the plasmid pAGV 40 (Herrera-Estrella et al., (1983) Nature 303, 209-213) and after addition of SphI linkers to the PvuII site between the SphI-Hind III sites of the polylinker of BIN 19 has been cloned was (s. 1 ).

Das Plasmid p35s-BE wurde mit Hilfe des Agrobakterien-Systems in Kartoffeln transferiert. Anschließend wurden ganze Pflanzen regeneriert. Proteinextrakte, die aus Knollen dieser Pflanzen isoliert worden waren, wurden mit Hilfe der Westernblot-Analyse auf das Vorhandensein des Verzweigungsenzyms getestet. Des weiteren wurden Knollen dieser Pflanzen auf den Gehalt an Amylose und Amylopektin untersucht.The Plasmid p35s-BE was in potatoes using the Agrobacterium system transferred. Subsequently whole plants were regenerated. Protein extracts from tubers of these plants were isolated by Western blot analysis tested for the presence of the branching enzyme. Furthermore tubers of these plants were based on the content of amylose and amylopectin examined.

Ausführungsbeispiel 2Embodiment 2

Herstellung des Plasmids p35s-anti-BE und Einführung des Plasmids in das pflanzliche Genom der KartoffelPreparation of the plasmid p35s-anti-BE and introduction of the plasmid into the vegetable genome of the potato

In analoger Weise, wie unter Ausführungsbeispiel 1 beschrieben, aber nun mehr mit einer in invertierter Richtung angeordneten cDNA des Verzweigungs enzyms wurde das Plasmid p35s-anti-BE hergestellt. Das Plasmid p35s-anti-BE hat eine Größe von 13,6 kb und besteht aus den drei Fragmenten A, B und C, die in die Schnittstellen des Polylinkers von BIN19 kloniert wurden.In analogous manner, as under embodiment 1, but now more with one in an inverted direction arranged cDNA of the branching enzyme was the plasmid p35s-anti-BE produced. The plasmid p35s-anti-BE has a size of 13.6 kb and consists of the three fragments A, B and C, which are in the interfaces of the polylinker of BIN19.

Fragment A (529 bp) beinhaltet den 35S Promotor des Blumenkohl-Mosaik-Virus (CaMV). Das Fragment umfaßt die Nukleotide 6909 bis 7437 des CaMV (Franck et al., Cell 21, 285-294), und wurde als EcoRI-KpnI-Fragment aus dem Plas mid pDH51 (Pietrzak et al., Nucleic Acids Research 14, 5857-5868) isoliert und zwischen die EcoRI-KpnI-Schnittstellen des Polylinkers des Plasmids BIN 19 kloniert.fragment A (529 bp) contains the cauliflower mosaic virus 35S promoter (CaMV). The fragment comprises nucleotides 6909 to 7437 of CaMV (Franck et al., Cell 21, 285-294), and was expressed as an EcoRI-KpnI fragment from plasmid pDH51 (Pietrzak et al., Nucleic Acids Research 14, 5857-5868) and between the EcoRI-KpnI sites of the polylinker of plasmid BIN19 cloned.

Fragment 8 enthält das 2909 bp große cDNA Fragment cBE, welches für das Verzweigungssystem kodiert. Es wurde als HindIII-Sma-I-Fragment aus dem Vektor pUC 19 herausgeschnitten und nach Auffüllen der HindIII-Stelle mit DNA Polymerase in die SmaI-Stelle des Polylinkers von BIN 19 kloniert. Da bei wurde die Orientierung so gewählt, daß der nicht-kodierende Strang ab gelesen wurde, was zur Bildung einer anti-sense RNA führt. Die Schnitt stellen SphI, PstI und XbaI, BamHI, SmaI stammen aus dem Polylinker von pUC 19. Die Schnittstellen BamHI/XbaI/SalI/PstI stammen aus dem Polylinker von BIN 19. Die auf dem Fragment B enthaltenen zwei EcoRI-Schnittstellen sind interne Schnittstellen des Fragments.fragment 8 contains the 2909 bp big cDNA fragment cBE, which is for the branching system encodes. It was called HindIII-Sma-I fragment cut out of the vector pUC 19 and after filling the HindIII site with DNA polymerase into the SmaI site of the polylinker cloned from BIN 19. Since the orientation was chosen so that the non-coding Strand was read, resulting in the formation of an anti-sense RNA. The Cut SphI, PstI and XbaI, BamHI, SmaI are from the Polylinker of pUC 19. The interfaces BamHI / XbaI / SalI / PstI originate from the polylinker of BIN 19. The fragments B contained on the fragment two EcoRI interfaces are internal fragments of the fragment.

Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5 (Gielen et al., EMBO J. 3, 835-846), Nukleotide 11749-11939), welches als PvuII-HindIII-Fragment aus dem Plasmid pAGV 40 (Herrera-Estrella et al. (1983) Nature 303, 209-213) isoliert worden ist und nach Addition von Sph-I-Linkern an die Pvu-II-Schnittstelle zwischen die SphI-HindIII-Schnittstellen des Polylinkers von BIN 19 kloniert worden war (siehe 2).Fragment C (192 bp) contains the polyadenylation signal of gene 3 of the T-DNA of the Ti plasmid pTiACH5 (Gielen et al., EMBO J. 3, 835-846), nucleotides 11749-11939), which is a PvuII-HindIII fragment from the plasmid pAGV 40 (Herrera-Estrella et al. (1983) Nature 303, 209-213) and after addition of Sph-I linkers to the Pvu II site between the SphI-HindIII sites of the polylinker had been cloned from BIN 19 (see 2 ).

Das Plasmid p35s-anti-BE wurde mit Hilfe des Agrobakterien-Systems in Kartoffeln transferiert. Anschließend wurden ganze Pflanzen regeneriert.The Plasmid p35s-anti-BE was purified by the Agrobacterium system in Transferred potatoes. Subsequently, whole plants were regenerated.

Proteinextrakte, die aus Knollen dieser Pflanzen isoliert worden waren, wurden mit Hilfe der Westernblot-Analyse auf das Vorhandensein des Verzweigungsenzyms getestet. Des weiteren wurden Knollen dieser Pflanzen auf den Gehalt an Amylose und Amylopektin untersucht.Protein extracts, which had been isolated from tubers of these plants were with Western blot analysis for the presence of the branching enzyme tested. Furthermore, tubers of these plants were based on the content investigated for amylose and amylopectin.

Ausführungsbeispiel 3Embodiment 3

Herstellung des Plasmids p33-BE und Einführung des Plasmids in das pflanzliche Genom der KartoffelPreparation of the plasmid p33-BE and introduction of the plasmid into the vegetable genome of the potato

In analoger Weise, wie im Anwendungsbeispiel 1 beschrieben, jedoch unter Austausch des 35s Promotors gegen den Promotor des Klasse I Patatin-Gens B33 (Rocha-Sosa et al., EMBO J. 8, 23-29) wurde das Plasmid p33-BE hergestellt. Das Plasmid p33-BE hat eine Größe von 14,6 kb und besteht aus den drei Fragmenten A, B und C, die in die Schnittstellen des Polylinkers von BIN 19 kloniert wurden.In analogous manner, as described in Application Example 1, however with replacement of the 35s promoter against the promoter of the class Patatin gene B33 (Rocha-Sosa et al., EMBO J. 8, 23-29) became the plasmid p33-BE produced. The plasmid p33-BE has a size of 14.6 kb and consists of the three fragments A, B and C, which are in the interfaces of the polylinker of BIN 19 were cloned.

Fragment A enthält das DraI-DraI-Fragment (Position –1512 bis Position +14) der Promotorregion des Patatin-Gens B33 (Rocha-Sosa et al., EMBO J. 8, 23-29), welches zunächst in die SacI-Stelle des Polylinkers von pUC 18 kloniert worden war. Dazu wurde der 3'-Überhang der Sac-I-Schnittstelle mittels T4-DNA Polymerase stumpf gemacht. Das Fragment wurde anschließend als EcoRI-BamHI-Fragment zwischen die EcoRI-Bam-HI-Stelle des Polylinkers von BIN 19 eingesetzt.fragment A contains the DraI-DraI fragment (position -1512 to position +14) of the Promoter region of the patatin gene B33 (Rocha-Sosa et al., EMBO J. 8, 23-29), which first into the SacI site of the polylinker of pUC18. This was the 3'-overhang made the Sac I site blunt by T4 DNA polymerase. The fragment was subsequently as EcoRI-BamHI fragment between the EcoRI-Bam HI site of the polylinker used by BIN 19.

Fragment B enthält das 2909 bp große cDNA Fragment cBE, welches für das Verzweigungsenzym kodiert. Es wurde als HindIII-SmaI-Fragment aus dem Vektor pUC 19 herausgeschnitten und nach Auffüllen der HindIII-Stelle mit DNA Polymerase in die SmaI-Stelle des Polylinkers von BIN 19 kloniert. Dabei wurde die Orientierung der cDNA so gewählt, daß der kodierende Strang abgelesen und damit eine sense-RNA gebildet wird. Die Schnittstellen BamHI/XbaI und PstI/SphI stammen aus dem Polylinker von pUC19. Die Schnittstellen BamHI/XbaI/SalI/PstI stammen aus dem Polylinker von BIN 19. Die auf dem Fragment B enthaltenen zwei EcoRI-Schnittstellen sind interne Schnittstellen dieses Fragments.fragment B contains the 2909 bp big cDNA fragment cBE, which is for encodes the branching enzyme. It was called HindIII-SmaI fragment cut out of the vector pUC 19 and after filling the HindIII site with DNA polymerase into the SmaI site of the polylinker of BIN 19 cloned. The orientation of the cDNA was chosen so that the coding Strang read and thus a sense RNA is formed. The interfaces BamHI / XbaI and PstI / SphI are from the polylinker of pUC19. The Interfaces BamHI / XbaI / SalI / PstI are from the polylinker of BIN 19. The two EcoRI sites contained on fragment B are internal interfaces of this fragment.

Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5 (Gielen et al., EMBO J. 3, 835-846, Nukleotide 11749-11939), welches als Pvu-II-HindIII-Fragment aus dem Plasmid pAGV 40 (Herrera-Estrella et al. (1983) Nature 303, 209-213) isoliert worden ist und nach Addition von SphI-Linkern an die PvuII-Schnittstelle zwischen die SphI-HindIII-Schnittstellen des Polylinkers von BIN 19 kloniert worden war.fragment C (192 bp) contains the polyadenylation signal of gene 3 of T-DNA of the Ti plasmid pTiACH5 (Gielen et al., EMBO J. 3, 835-846, Nucleotides 11749-11939), which is a Pvu II-HindIII fragment the plasmid pAGV 40 (Herrera-Estrella et al. (1983) Nature 303, 209-213) and after addition of SphI linkers to the PvuII site between the SphI-HindIII sites of the polylinker of BIN 19 had been cloned.

Das Plasmid p33-BE wurde in Agrobacterium tumefaciens überführt und zur Transformation von Kartoffelpflanzen eingesetzt.The Plasmid p33-BE was transferred to Agrobacterium tumefaciens and used for the transformation of potato plants.

Ausführungsbeispiel 4Embodiment 4

Herstellung des Plasmids p33-anti-BE und Einführung des Plasmids in das pflanzliche Genom der KartoffelPreparation of the plasmid p33-anti-BE and introduction of the plasmid into the vegetable genome of the potato

In analoger Weise, wie im Ausführungsbeispiel 2 beschrieben, jedoch unter Austausch des 35S-Promotors gegen den Promotor des Klasse I Patatin-Gens B33 (Rocha-Sosa et al., EMBO J. 8, 23-29) wurde das Plasmid p33-anti-BE hergestellt. Das Plasmid p33-anti-BE hat eine Größe von 14,6 kb und besteht aus den drei Fragmenten A, B und C, die in die Schnittstellen des Polylinkers von BIN 19 kloniert wurden.In analogous manner, as in the embodiment 2, but with replacement of the 35S promoter against the Promoter of class I patatin gene B33 (Rocha-Sosa et al., EMBO J. 8, 23-29), the plasmid p33-anti-BE was prepared. The plasmid p33-anti-BE has a size of 14.6 kb and consists of the three fragments A, B and C, which are in the interfaces of the polylinker of BIN 19 were cloned.

Fragment A enthält das DraI-DraI-Fragment (Position –1512 bis Position +14) der Promotorregion des Patatin-Gens B33 (Rocha-Sosa et al., EMBO J. 8, 23-29) welches zunächst in die SacI-Stelle des Polylinkers von pUC 18 kloniert worden war. Dazu wurde der 3'-Überhang der SacI-Schnittstelle mittels T4-DNA Polymerase stumpf gemacht. Das Fragment wurde sodann als EcoRI-BamHI-Fragment zwischen die EcoRI-BamHI-Stelle des Polylinkers von BIN 19 eingesetzt.fragment A contains the DraI-DraI fragment (position -1512 to position +14) of the Promoter region of the patatin gene B33 (Rocha-Sosa et al., EMBO J. 8, 23-29) which first into the SacI site of the polylinker of pUC18. This was the 3'-overhang made the SacI site blunt by T4 DNA polymerase. The fragment was then inserted as an EcoRI-BamHI fragment between the EcoRI-BamHI site of the polylinker of BIN 19 used.

Fragment B enthält das 2909 bp große cDNA Fragment cBE, welches für das Verzweigungsenzym kodiert. Es wurde als HindIII-SmaI-Fragment aus dem Vektor pUC 18 herausgeschnitten und nach Auffüllen der HindIII-Stelle mit DNAPolymerase in die SmaI-Stelle des Polylinkers von BIN 19 kloniert. Dabei wurde die Orientierung der cDNA so gewählt, daß der nichtkodierende Strang abgelesen und damit eine anti-sense-RNA gebildet wird. Die Schnittstellen SphI, PstI und XbaI, BamHI, SmaI stammen aus dem Polylinker von pUC 19. Die Schnittstellen BamHI/XbaI/SalI/PstI stammen aus dem Polylinker von BIN 19. Die auf dem Fragment B enthaltenen zwei EcoRI-Schnittstellen sind interne Schnittstellen des Fragments.fragment B contains the 2909 bp big cDNA fragment cBE, which is for encodes the branching enzyme. It was called HindIII-SmaI fragment cut out of the vector pUC 18 and after filling the HindIII site with DNAPolymerase into the SmaI site of the polylinker of BIN 19 cloned. The orientation of the cDNA was chosen so that the non-coding Strand read and thus an anti-sense RNA is formed. The Interfaces SphI, PstI and XbaI, BamHI, SmaI are from the Polylinker of pUC 19. The interfaces BamHI / XbaI / SalI / PstI originate from the polylinker of BIN 19. The fragments B contained on the fragment two EcoRI interfaces are internal fragments of the fragment.

Fragment C (192 bp) beinhaltet das Polyadenylierungssignal des Gens 3 der T-DNA des Ti-Plasmids pTiACH5 (Gielen et al., EMBO J. 3, 835-846), Nukleotide 11749-11939), welches als PvuII-HindIII-Fragment aus dem Plasmid pAGV 40 (Herrera-Estrella et al. (1983), Nature 303, 209-213) isoliert worden ist und nach Addition von SphI-Linkern an die PvuII-Schnittstelle zwischen die SphI-HindIII-Schnittstellen des Polylinkers von BIN 19 kloniert worden war.fragment C (192 bp) contains the polyadenylation signal of gene 3 of T-DNA of the Ti plasmid pTiACH5 (Gielen et al., EMBO J. 3, 835-846), Nucleotides 11749-11939), which expresses as PvuII-HindIII fragment the plasmid pAGV 40 (Herrera-Estrella et al. (1983), Nature 303, 209-213) and after addition of SphI linkers to the PvuII site between the SphI-HindIII sites of the polylinker of BIN 19 had been cloned.

Das Plasmid p33-anti-BE wurde in Agrobacterium tumefaciens überführt und zur Transformation von Kartoffelpflanzen eingesetzt.The Plasmid p33-anti-BE was transformed into Agrobacterium tumefaciens and used for the transformation of potato plants.

Ausführungsbeispiel 5Embodiment 5

Von der im Ausführungsbeispiel 1 aufgezeigten 2909 bp langen cDNA-Sequenz, die in der HindII-Schnittstelle des Klonierungsvektors pUC19 für das Ver zweigungsenzym kodiert, wurden die Nukleotide 166-2909 in die entsprechenden Schnittstellen des Polylinkers des Klonierungsvektors pUC18 insertiert. Dies ermöglichte eine Fusion des N-Terminus des auf dem Vektor kodierten α-Peptids der β-Galactosidase mit einem Teil des Verzweigungsenzyms. Die Funktionalität des entstehenden Fusionsproteins wurde in einer für das Verzweigungsenzym defizienten Mutante von Escherichia coli (KV 832) (Kiel et al., Gene 78, 9-17) überprüft. Die mit diesem Konstrukt transformierten Zellen wurden auf 0,5 % Glucose enthaltenden YT-Agarplatten plattiert. Die entstandenen Kolonien wurden mit Lugolscher Lösung angefärbt. Die Transformanden zeigten im Gegensatz zu den blaufärbenden untransformierten Zellen eine gelb-rötliche Färbung, was die Verzweigungsaktivität des Fusionsproteins belegt (Kiel et al., Gene 78, 9-17). Eine Überproduktion dieses Proteins in Escherichia coli würde den Einsatz als technisches Enzym ermöglichen.From in the embodiment 1 demonstrated 2909 bp cDNA sequence encoding the HindII site of the cloning vector pUC19 for the branching enzyme encodes, nucleotides 166-2909 in the corresponding interfaces of the polylinker of the cloning vector pUC18 inserted. This enabled a fusion of the N-terminus of the α-peptide encoded on the vector the β-galactosidase with a part of the branching enzyme. The functionality of the resulting Fusion protein was used in one for the Branching enzyme deficient mutant of Escherichia coli (KV 832) (Kiel et al., Gene 78, 9-17). The cells transformed with this construct were reduced to 0.5% glucose containing plated YT agar plates. The resulting colonies were using Lugolscher solution stained. The transformants showed in contrast to the blue-coloring untransformed cells have a yellow-reddish color, indicating the branching activity of the fusion protein (Kiel et al., Gene 78, 9-17). An overproduction of this protein in Escherichia coli would allow use as a technical enzyme.

Claims (9)

Plasmid enthaltend eine DNA-Sequenz, deren in dieser Sequenz enthaltene Information zu einer Veränderung der Karbohydratkonzentration und Karbohydratzusammensetzung in Pflanzen führt, dadurch gekennzeichnet, dass die DNA-Sequenz die kodierende Sequenz eines Branching-Enzyms aus Kartoffelknollen ist.A plasmid containing a DNA sequence whose information contained in this sequence results in a change in the carbohydrate concentration and carbohydrate composition in plants, characterized in that the DNA sequence is the coding sequence of a potato tuber Branching enzyme. Plasmid gemäß Anspruch 1, dadurch gekennzeichnet, dass die DNA-Sequenz die Sequenz
Figure 00240001
Figure 00250001
hat. Plasmid according to claim 1, characterized in that the DNA sequence is the sequence
Figure 00240001
Figure 00250001
Has. Plasmid gemäß den Ansprüchen 1 oder 2, dadurch gekennzeichnet, dass die DNA-Sequenz des Branching Enzyms an die regulatorischen Sequenzen anderer Gene, die eine Transkription der das Branching-Enzym kodierenden DNA-Sequenz sicherstellen, fusioniert ist.A plasmid according to claims 1 or 2, characterized in that the DNA sequence of the Branching enzyme to the regulatory sequences of other genes encoding a transcription of the Branching-En Ensuring the encoding sequence of the DNA is fused. Plasmid gemäß Anspruch 3, dadurch gekennzeichnet, dass die DNA-Sequenz des Branching-Enzyms in invertierter Anordnung an die regulatorischen Sequenzen anderer Gene fusioniert ist, wobei das 3'-Ende der kodierenden Sequenz an das 3'-Ende des Promotors und das 5'-Ende der kodierenden Sequenzen an das 5'-Ende des Terminationssignals fusioniert ist.Plasmid according to claim 3, characterized in that the DNA sequence of the Branching enzyme in inverted arrangement to the regulatory sequences of others Gene is fused, with the 3 'end the coding sequence to the 3 'end of the promoter and the 5 'end of the coding sequences fused to the 5 'end of the termination signal is. Plasmid gemäß Anspruch 3 oder 4, dadurch gekennzeichnet, dass die regulatorischen Sequenzen Promotoren und Terminationssignale von pflanzlichen Genen sind.Plasmid according to claim 3 or 4, characterized in that the regulatory sequences Promoters and termination signals of plant genes are. Plasmid gemäß den Ansprüchen 3 oder 4, dadurch gekennzeichnet, dass die regulatorischen Sequenzen Promotoren und Terminationssignale von viralen Genen sind.Plasmid according to claims 3 or 4, characterized in that the regulatory sequences promoters and termination signals from viral genes. Plasmid gemäß Anspruch 5, dadurch gekennzeichnet, dass der Promotor der Promotor des Klasse I Patatin-Gens B33 ist.Plasmid according to claim 5, characterized in that the promoter is the promoter of the class I is Patatin gene B33. Pflanze oder Pflanzenzelle enthaltend mindestens eines der Plasmide gemäß den Ansprüchen 1 bis 7.Plant or plant cell containing at least one of the plasmids according to claims 1 to 7th Verwendung der Plasmide gemäß den Ansprüchen 1 bis 7 zur Herstellung von transgenen Pflanzen deren Amylose-/Amylopektin-Verhältnis verändert ist. Use of the plasmids according to claims 1 to 7 for the preparation of transgenic plants whose amylose / amylopectin ratio is altered.
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US08/104,158 US6215042B1 (en) 1991-02-13 1992-02-11 Plasmids containing DNA-sequences that cause changes in the carbohydrate concentration and carbohydrate composition in plants, as well as plant cells and plants containing these plasmids
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