DE3248145A1 - Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym - Google Patents

Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym

Info

Publication number
DE3248145A1
DE3248145A1 DE19823248145 DE3248145A DE3248145A1 DE 3248145 A1 DE3248145 A1 DE 3248145A1 DE 19823248145 DE19823248145 DE 19823248145 DE 3248145 A DE3248145 A DE 3248145A DE 3248145 A1 DE3248145 A1 DE 3248145A1
Authority
DE
Germany
Prior art keywords
sarcosine
carbamoylsarcosine
amidohydrolase
determination
dsm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19823248145
Other languages
German (de)
English (en)
Inventor
Rolf Dr.Rer.Nat. Deeg
Helmgard 8132 Tutzing Gauhl
Albert Dr.rer.nat. 8124 Seeshaupt Röder
Joachim Dr.rer.nat. 8131 Bernried Siedel
Joachim Dr.Rer.Nat. 8130 Starnberg Ziegenhorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE19823248145 priority Critical patent/DE3248145A1/de
Priority to AU21870/83A priority patent/AU545903B2/en
Priority to IE2856/83A priority patent/IE56346B1/en
Priority to ES528117A priority patent/ES8406541A1/es
Priority to US06/562,072 priority patent/US4645739A/en
Priority to ZA839522A priority patent/ZA839522B/xx
Priority to DD83258475A priority patent/DD216255A5/de
Priority to DK198305925A priority patent/DK173112B1/da
Priority to DD83268005A priority patent/DD222631A5/de
Priority to IL70529A priority patent/IL70529A/xx
Priority to CA000444052A priority patent/CA1210675A/en
Priority to AT83113075T priority patent/ATE28758T1/de
Priority to JP58242347A priority patent/JPS59132891A/ja
Priority to EP83113075A priority patent/EP0112571B1/de
Priority to DE8383113075T priority patent/DE3372870D1/de
Publication of DE3248145A1 publication Critical patent/DE3248145A1/de
Priority to JP62196139A priority patent/JPS63102680A/ja
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/70—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving creatine or creatinine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/98—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/83—Arthrobacter
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/859—Micrococcus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
DE19823248145 1982-12-27 1982-12-27 Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym Withdrawn DE3248145A1 (de)

Priority Applications (16)

Application Number Priority Date Filing Date Title
DE19823248145 DE3248145A1 (de) 1982-12-27 1982-12-27 Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym
AU21870/83A AU545903B2 (en) 1982-12-27 1983-12-01 Determination of n-carbamoylsarcosine using n-carbamoyl- sarcosine-amidohydrolase
IE2856/83A IE56346B1 (en) 1982-12-27 1983-12-05 Process and reagent for the determination of n-carbamoylsarcosine and new enzyme suitable therefor
ES528117A ES8406541A1 (es) 1982-12-27 1983-12-16 Procedimiento para la determinacion de n-carbamoil-sarcosina
US06/562,072 US4645739A (en) 1982-12-27 1983-12-16 Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme
DK198305925A DK173112B1 (da) 1982-12-27 1983-12-22 N-carbamoylsarcosin-amidohydrolase og en fremgangsmåde til udvinding deraf, en fremgangsmåde til bestemmelse af N-carbamoyl
DD83258475A DD216255A5 (de) 1982-12-27 1983-12-22 Verfahren und reagenz zur bestimmung von n-carbamoylsacosin
ZA839522A ZA839522B (en) 1982-12-27 1983-12-22 Process and reagent for determining n-carbamoylsarcosine
DD83268005A DD222631A5 (de) 1982-12-27 1983-12-22 Verfahren zur gewinnung von n-carbamoylsarcosin-amidohydrolase
IL70529A IL70529A (en) 1982-12-27 1983-12-22 N-carbamoylsarcosine-amido-hydrolase,its preparation and process and reagent containing it for the determination of n-carbamoylsarcosine
CA000444052A CA1210675A (en) 1982-12-27 1983-12-22 Process and reagent for the determination of n- carbamoylsarcosine
AT83113075T ATE28758T1 (de) 1982-12-27 1983-12-23 Verfahren und reagenz zur bestimmung von ncarbamoylsarcosin und hierfuer geeignetes neues enzym.
JP58242347A JPS59132891A (ja) 1982-12-27 1983-12-23 Ｎ−カルバモイルサルコシンの測定法及び測定試薬
EP83113075A EP0112571B1 (de) 1982-12-27 1983-12-23 Verfahren und Reagenz zur Bestimmung von N-Carbamoylsarcosin und hierfür geeignetes neues Enzym
DE8383113075T DE3372870D1 (en) 1982-12-27 1983-12-23 Method and reagent for the determination of n-carbamoylsarcosine, and an enzyme therefor
JP62196139A JPS63102680A (ja) 1982-12-27 1987-08-05 Ｎ−カルバモイルサルコシン−アミドヒドロラ−ゼ及びその取得法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823248145 DE3248145A1 (de) 1982-12-27 1982-12-27 Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym

Publications (1)

Publication Number Publication Date
DE3248145A1 true DE3248145A1 (de) 1984-06-28

Family

ID=6181852

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19823248145 Withdrawn DE3248145A1 (de) 1982-12-27 1982-12-27 Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym
DE8383113075T Expired DE3372870D1 (en) 1982-12-27 1983-12-23 Method and reagent for the determination of n-carbamoylsarcosine, and an enzyme therefor

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8383113075T Expired DE3372870D1 (en) 1982-12-27 1983-12-23 Method and reagent for the determination of n-carbamoylsarcosine, and an enzyme therefor

Country Status (13)

Country Link
US (1) US4645739A (cg-RX-API-DMAC10.html)
EP (1) EP0112571B1 (cg-RX-API-DMAC10.html)
JP (2) JPS59132891A (cg-RX-API-DMAC10.html)
AT (1) ATE28758T1 (cg-RX-API-DMAC10.html)
AU (1) AU545903B2 (cg-RX-API-DMAC10.html)
CA (1) CA1210675A (cg-RX-API-DMAC10.html)
DD (2) DD222631A5 (cg-RX-API-DMAC10.html)
DE (2) DE3248145A1 (cg-RX-API-DMAC10.html)
DK (1) DK173112B1 (cg-RX-API-DMAC10.html)
ES (1) ES8406541A1 (cg-RX-API-DMAC10.html)
IE (1) IE56346B1 (cg-RX-API-DMAC10.html)
IL (1) IL70529A (cg-RX-API-DMAC10.html)
ZA (1) ZA839522B (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816393A (en) * 1984-02-24 1989-03-28 Boehringer Mannheim Gmbh Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4812399A (en) * 1986-04-21 1989-03-14 Eastman Kodak Company Analytical element and method for the determination of creatinine or creatine
DE3821077A1 (de) * 1988-02-12 1989-08-24 David Diagnostics Inc Verfahren zur bestimmung des sauerstoffgehaltes in biologischen fluessigsubstraten und vorrichtung zur durchfuehrung des verfahrens
DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
DE4103220A1 (de) * 1991-02-02 1992-08-06 Boehringer Mannheim Gmbh Verfahren zur stabilisierung von 1-methylhydantoinase, verwendung einer stabilisierten 1-methylhydantoinase zur bestimmung eines analyten, entsprechendes bestimmungsverfahren sowie hierfuer geeignete mittel
BR9600672A (pt) * 1996-03-08 1997-12-30 Dedini S A Administracao E Par Processo de hidrólise ácido de material lignocelulósico e reator de hidrólise

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2122294C3 (de) * 1971-05-05 1979-08-16 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Gewinnung von Creatininamidohydrolase
US4039384A (en) * 1975-04-05 1977-08-02 Noda Institute For Scientific Research Creatinine amidohydrolase and creatine amidinohydrolase and process for producing them
JPS603480B2 (ja) * 1980-11-10 1985-01-28 東洋紡績株式会社 クレアチニンおよびクレアチンの定量法
IT1137075B (it) * 1981-05-28 1986-09-03 Chemical Lab S R L Procedimento per la determinazione della creattinina (creatininio) in liquidi biologici, e reattivo per la sua attuazione

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816393A (en) * 1984-02-24 1989-03-28 Boehringer Mannheim Gmbh Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof

Also Published As

Publication number Publication date
DE3372870D1 (en) 1987-09-10
JPS639839B2 (cg-RX-API-DMAC10.html) 1988-03-02
EP0112571B1 (de) 1987-08-05
JPS59132891A (ja) 1984-07-31
IE832856L (en) 1984-06-27
CA1210675A (en) 1986-09-02
DK173112B1 (da) 2000-01-31
JPH047673B2 (cg-RX-API-DMAC10.html) 1992-02-12
AU545903B2 (en) 1985-08-08
DD216255A5 (de) 1984-12-05
DD222631A5 (de) 1985-05-22
ZA839522B (en) 1984-09-26
IE56346B1 (en) 1991-07-03
ES528117A0 (es) 1984-08-01
DK592583A (da) 1984-06-28
DK592583D0 (da) 1983-12-22
US4645739A (en) 1987-02-24
AU2187083A (en) 1984-07-05
ATE28758T1 (de) 1987-08-15
EP0112571A1 (de) 1984-07-04
IL70529A (en) 1987-10-20
IL70529A0 (en) 1984-03-30
JPS63102680A (ja) 1988-05-07
ES8406541A1 (es) 1984-08-01

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