DE3110031A1 - Verschmolzene gene, ihre herstellung und verwendung derselben - Google Patents
Verschmolzene gene, ihre herstellung und verwendung derselbenInfo
- Publication number
- DE3110031A1 DE3110031A1 DE19813110031 DE3110031A DE3110031A1 DE 3110031 A1 DE3110031 A1 DE 3110031A1 DE 19813110031 DE19813110031 DE 19813110031 DE 3110031 A DE3110031 A DE 3110031A DE 3110031 A1 DE3110031 A1 DE 3110031A1
- Authority
- DE
- Germany
- Prior art keywords
- gene
- fused
- polypeptide
- coding
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 129
- 238000004519 manufacturing process Methods 0.000 title description 5
- 229920001184 polypeptide Polymers 0.000 claims description 76
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 76
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 76
- 239000012634 fragment Substances 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 19
- 244000005700 microbiome Species 0.000 claims description 17
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 10
- 125000003277 amino group Chemical group 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 6
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 4
- 238000010367 cloning Methods 0.000 claims description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 102000002464 Galactosidases Human genes 0.000 claims 1
- 108010093031 Galactosidases Proteins 0.000 claims 1
- 108700009124 Transcription Initiation Site Proteins 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 239000013612 plasmid Substances 0.000 description 15
- 230000015572 biosynthetic process Effects 0.000 description 8
- 230000008878 coupling Effects 0.000 description 8
- 238000010168 coupling process Methods 0.000 description 8
- 238000005859 coupling reaction Methods 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 101150109249 lacI gene Proteins 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 3
- 101150066555 lacZ gene Proteins 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 101000991414 Aspergillus oryzae (strain ATCC 42149 / RIB 40) Nuclease S1 Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101710081048 Endonuclease III Proteins 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 239000002962 chemical mutagen Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 102000018146 globin Human genes 0.000 description 1
- 108060003196 globin Proteins 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical class ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- 238000010397 one-hybrid screening Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 101150016309 trpC gene Proteins 0.000 description 1
- 101150100816 trpD gene Proteins 0.000 description 1
- 101150079930 trpGD gene Proteins 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13115280A | 1980-03-17 | 1980-03-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3110031A1 true DE3110031A1 (de) | 1982-01-07 |
Family
ID=22448125
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19813110031 Withdrawn DE3110031A1 (de) | 1980-03-17 | 1981-03-16 | Verschmolzene gene, ihre herstellung und verwendung derselben |
| DE19813153267 Pending DE3153267A1 (https=) | 1980-03-17 | 1981-03-16 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19813153267 Pending DE3153267A1 (https=) | 1980-03-17 | 1981-03-16 |
Country Status (11)
| Country | Link |
|---|---|
| JP (1) | JPS56167698A (https=) |
| AT (1) | AT378004B (https=) |
| BE (1) | BE887991A (https=) |
| CA (1) | CA1198385A (https=) |
| DE (2) | DE3110031A1 (https=) |
| ES (2) | ES8205432A1 (https=) |
| FR (1) | FR2478125B1 (https=) |
| GB (1) | GB2071671B (https=) |
| IT (1) | IT1144705B (https=) |
| NL (1) | NL8101289A (https=) |
| SE (1) | SE8101665L (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4874702A (en) * | 1980-09-08 | 1989-10-17 | Biogen, Inc. | Vectors and methods for making such vectors and for expressive cloned genes |
| FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
| IL66263A (en) * | 1981-08-10 | 1985-12-31 | Genex Corp | Expression vectors for introducing a gene into a procaryotic organism |
| US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
| EP0109428B1 (en) * | 1982-05-25 | 1988-04-06 | Brandeis University | Cloning vector with indicator genes which have been inactivated by frameshift |
| US4582800A (en) * | 1982-07-12 | 1986-04-15 | Hoffmann-La Roche Inc. | Novel vectors and method for controlling interferon expression |
| JPS5939899A (ja) * | 1982-08-27 | 1984-03-05 | Gakuzo Tamura | 新規ベクタ− |
| ATE60803T1 (de) * | 1983-06-27 | 1991-02-15 | Genentech Inc | Uebertragbare induzierbare kontrollsysteme, diese enthaltende expressionsvektoren, mit diesen transformierte mikroorganismen und ihre verwendung bei der expression von exogenem protein. |
| DE3587730T2 (de) * | 1984-02-08 | 1994-05-05 | Cetus Oncology Corp | Kontrollsysteme für rekombinant-manipulationen. |
| US4923808A (en) * | 1985-03-12 | 1990-05-08 | Genentech, Inc. | Method for identifying mutants secreting high levels of heterologous proteins |
| IL78775A (en) * | 1985-05-15 | 1992-06-21 | Biotech Australia Pty Ltd | Oral vaccines |
| US5030576A (en) * | 1986-04-30 | 1991-07-09 | Genentech, Inc. | Receptors for efficient determination of ligands and their antagonists or agonists |
| US4859609A (en) * | 1986-04-30 | 1989-08-22 | Genentech, Inc. | Novel receptors for efficient determination of ligands and their antagonists or agonists |
| JP6297223B2 (ja) | 2015-06-23 | 2018-03-20 | 富士フイルム株式会社 | 電気音響変換フィルムおよび電気音響変換器 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2862496D1 (en) * | 1977-11-08 | 1989-06-29 | Genentech Inc | Method for polypeptide production involving expression of a heterologous gene, recombinant microbial cloning vehicle containing said gene and bacterial culture transformed by said cloning vehicle |
| FI792481A7 (fi) * | 1978-08-11 | 1981-01-01 | The Regents Of The Univ Of | Enkaroyyttisen proteiinin synteesi mikro-organismia käyttäen. |
| NL8000127A (nl) * | 1979-01-15 | 1980-07-17 | Harvard College | Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen. |
-
1981
- 1981-03-16 IT IT67359/81A patent/IT1144705B/it active
- 1981-03-16 ES ES500397A patent/ES8205432A1/es not_active Expired
- 1981-03-16 SE SE8101665A patent/SE8101665L/ not_active Application Discontinuation
- 1981-03-16 GB GB8108136A patent/GB2071671B/en not_active Expired
- 1981-03-16 DE DE19813110031 patent/DE3110031A1/de not_active Withdrawn
- 1981-03-16 DE DE19813153267 patent/DE3153267A1/de active Pending
- 1981-03-16 CA CA000373031A patent/CA1198385A/en not_active Expired
- 1981-03-17 BE BE0/204159A patent/BE887991A/fr not_active IP Right Cessation
- 1981-03-17 AT AT0122281A patent/AT378004B/de not_active IP Right Cessation
- 1981-03-17 NL NL8101289A patent/NL8101289A/nl not_active Application Discontinuation
- 1981-03-17 FR FR8105347A patent/FR2478125B1/fr not_active Expired
- 1981-03-17 JP JP3733881A patent/JPS56167698A/ja active Pending
-
1982
- 1982-03-01 ES ES510007A patent/ES8306177A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES510007A0 (es) | 1983-05-01 |
| GB2071671A (en) | 1981-09-23 |
| AT378004B (de) | 1985-06-10 |
| JPS56167698A (en) | 1981-12-23 |
| ES8306177A1 (es) | 1983-05-01 |
| ES500397A0 (es) | 1982-06-01 |
| FR2478125B1 (fr) | 1985-07-05 |
| ATA122281A (de) | 1984-10-15 |
| BE887991A (fr) | 1981-07-16 |
| NL8101289A (nl) | 1981-10-16 |
| CA1198385A (en) | 1985-12-24 |
| DE3153267A1 (https=) | 1985-08-14 |
| SE8101665L (sv) | 1981-09-18 |
| GB2071671B (en) | 1983-10-05 |
| IT1144705B (it) | 1986-10-29 |
| ES8205432A1 (es) | 1982-06-01 |
| IT8167359A0 (it) | 1981-03-16 |
| FR2478125A1 (fr) | 1981-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8128 | New person/name/address of the agent |
Representative=s name: HENKEL, G., DR.PHIL. FEILER, L., DR.RER.NAT. HAENZ |
|
| 8172 | Supplementary division/partition in: |
Ref country code: DE Ref document number: 3153267 Format of ref document f/p: P |
|
| Q171 | Divided out to: |
Ref country code: DE Ref document number: 3153267 |
|
| 8139 | Disposal/non-payment of the annual fee |