ES8306177A1 - Un metodo de proporcionar una muestra de un gen fusionado que codifica un polipeptido eucariotico o procariotico deseado. - Google Patents

Un metodo de proporcionar una muestra de un gen fusionado que codifica un polipeptido eucariotico o procariotico deseado.

Info

Publication number
ES8306177A1
ES8306177A1 ES510007A ES510007A ES8306177A1 ES 8306177 A1 ES8306177 A1 ES 8306177A1 ES 510007 A ES510007 A ES 510007A ES 510007 A ES510007 A ES 510007A ES 8306177 A1 ES8306177 A1 ES 8306177A1
Authority
ES
Spain
Prior art keywords
gene
fused
polypeptide
eukaryotic
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES510007A
Other languages
English (en)
Other versions
ES510007A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of ES510007A0 publication Critical patent/ES510007A0/es
Publication of ES8306177A1 publication Critical patent/ES8306177A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO PARA PROPORCIONAR UNA MUESTRA DE UN GEN FUSIONADO QUE CODIFICA UN POLIPEPTIDO EUCARIOTICO O PROCARIOTICO DESEADO Y QUE TIENE SU PROMOTOR TRANSPORTABLE LOCALIZADO A UNA DISTANCIA OPTIMA DE SU PUNTO DE INICIACION DE TRADUCCION. COMPRENDE LAS SIGUIENTES ETAPAS: PRIMERA, SE PREPARA UNA MUESTRA DE UN GEN FUSIONADO CAPAZ DE CODIFICAR UN POLIPEPTIDO HIBRIDO ENSAYABLE; SEGUNDA, SE INSERTA UN PROMOTOR TRANSPORTABLE A DISTANCIAS VARIABLES EN DICHO GEN FUSIONADO; TERCERA, SE SOMETE A CLONACION LA MUESTRA DE GEN FUSIONADO PARA OBTENER MICROORGANISMOS; CUARTA, SE SELECCIONA EL GEN FUSIONADO QUE PRODUCE LA CANTIDAD MAXIMA DE DICHO POLIPEPTIDO HIBRIDO ENSAYABLE; Y POR ULTIMO, SE RECONSTITUYE A PARTIR DEL GEN FUSIONADO SELECCIONADO, EL GEN QUE CODIFICA EL POLIPEPTIDO EUCARIOTICO O PROCARIOTICO DESEADO.
ES510007A 1980-03-17 1982-03-01 Un metodo de proporcionar una muestra de un gen fusionado que codifica un polipeptido eucariotico o procariotico deseado. Expired ES8306177A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13115280A 1980-03-17 1980-03-17

Publications (2)

Publication Number Publication Date
ES510007A0 ES510007A0 (es) 1983-05-01
ES8306177A1 true ES8306177A1 (es) 1983-05-01

Family

ID=22448125

Family Applications (2)

Application Number Title Priority Date Filing Date
ES500397A Granted ES500397A0 (es) 1980-03-17 1981-03-16 Un metodo de determinar la cantidad de un polipeptido enca- riotico o procariotico producido por un microorganismo
ES510007A Expired ES8306177A1 (es) 1980-03-17 1982-03-01 Un metodo de proporcionar una muestra de un gen fusionado que codifica un polipeptido eucariotico o procariotico deseado.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES500397A Granted ES500397A0 (es) 1980-03-17 1981-03-16 Un metodo de determinar la cantidad de un polipeptido enca- riotico o procariotico producido por un microorganismo

Country Status (11)

Country Link
JP (1) JPS56167698A (es)
AT (1) AT378004B (es)
BE (1) BE887991A (es)
CA (1) CA1198385A (es)
DE (2) DE3110031A1 (es)
ES (2) ES500397A0 (es)
FR (1) FR2478125B1 (es)
GB (1) GB2071671B (es)
IT (1) IT1144705B (es)
NL (1) NL8101289A (es)
SE (1) SE8101665L (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0109428B1 (en) * 1982-05-25 1988-04-06 Brandeis University Cloning vector with indicator genes which have been inactivated by frameshift
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
JPS5939899A (ja) * 1982-08-27 1984-03-05 Gakuzo Tamura 新規ベクタ−
EP0130074B1 (en) * 1983-06-27 1991-02-06 Genentech, Inc. Portable inducible control system, expression vectors containing them, microorganisms transformed with them, and their use in expressing exogenous protein
ATE100494T1 (de) * 1984-02-08 1994-02-15 Cetus Oncology Corp Kontrollsysteme fuer rekombinant-manipulationen.
US4923808A (en) * 1985-03-12 1990-05-08 Genentech, Inc. Method for identifying mutants secreting high levels of heterologous proteins
IL78775A (en) * 1985-05-15 1992-06-21 Biotech Australia Pty Ltd Oral vaccines
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
US5030576A (en) * 1986-04-30 1991-07-09 Genentech, Inc. Receptors for efficient determination of ligands and their antagonists or agonists
JP6297223B2 (ja) 2015-06-23 2018-03-20 富士フイルム株式会社 電気音響変換フィルムおよび電気音響変換器

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE871782A (fr) * 1977-11-08 1979-05-07 Genentech Inc Procede permettant l'expression microbienne de polypeptides et moyens appropries
FI792481A (fi) * 1978-08-11 1980-02-12 Univ California Syntes av enkaroytiskt protein genom anvaendning av mikro-organismer
NL8000127A (nl) * 1979-01-15 1980-07-17 Harvard College Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen.

Also Published As

Publication number Publication date
DE3153267A1 (es) 1985-08-14
NL8101289A (nl) 1981-10-16
IT8167359A0 (it) 1981-03-16
JPS56167698A (en) 1981-12-23
FR2478125B1 (fr) 1985-07-05
ES8205432A1 (es) 1982-06-01
DE3110031A1 (de) 1982-01-07
ATA122281A (de) 1984-10-15
AT378004B (de) 1985-06-10
GB2071671A (en) 1981-09-23
ES510007A0 (es) 1983-05-01
ES500397A0 (es) 1982-06-01
FR2478125A1 (fr) 1981-09-18
GB2071671B (en) 1983-10-05
BE887991A (fr) 1981-07-16
CA1198385A (en) 1985-12-24
SE8101665L (sv) 1981-09-18
IT1144705B (it) 1986-10-29

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19991108