DE2818841C2 - Fluorometer-Mikroskop - Google Patents

Fluorometer-Mikroskop

Info

Publication number
DE2818841C2
DE2818841C2 DE2818841A DE2818841A DE2818841C2 DE 2818841 C2 DE2818841 C2 DE 2818841C2 DE 2818841 A DE2818841 A DE 2818841A DE 2818841 A DE2818841 A DE 2818841A DE 2818841 C2 DE2818841 C2 DE 2818841C2
Authority
DE
Germany
Prior art keywords
microscope
fluorescence
wavelength
light beam
dichroic mirror
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2818841A
Other languages
German (de)
English (en)
Other versions
DE2818841A1 (de
Inventor
Mamoru Aihara
Youichi Hachioji Tokyo Kondo
Kazuhiko Nakamura
Ichiro Sawamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Optical Co Ltd filed Critical Olympus Optical Co Ltd
Publication of DE2818841A1 publication Critical patent/DE2818841A1/de
Application granted granted Critical
Publication of DE2818841C2 publication Critical patent/DE2818841C2/de
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Microscoopes, Condenser (AREA)
DE2818841A 1977-04-30 1978-04-28 Fluorometer-Mikroskop Expired DE2818841C2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5055877A JPS53135660A (en) 1977-04-30 1977-04-30 Fluorescent photometric microscope using laser light

Publications (2)

Publication Number Publication Date
DE2818841A1 DE2818841A1 (de) 1978-11-09
DE2818841C2 true DE2818841C2 (de) 1984-06-20

Family

ID=12862329

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2818841A Expired DE2818841C2 (de) 1977-04-30 1978-04-28 Fluorometer-Mikroskop

Country Status (3)

Country Link
US (1) US4284897A (US20020051482A1-20020502-M00012.png)
JP (1) JPS53135660A (US20020051482A1-20020502-M00012.png)
DE (1) DE2818841C2 (US20020051482A1-20020502-M00012.png)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3614359A1 (de) * 1985-07-26 1987-02-05 Jenoptik Jena Gmbh Anordnung zur bildlichen darstellung und analyse von fluoreszenzsignalen
DE3733573A1 (de) * 1987-10-03 1989-04-20 Leybold Ag Vorrichtung zum messen des fremdstoffanteils in stroemenden fluessigkeiten
DE4221063A1 (de) * 1992-06-26 1994-01-05 Thomas Dr Heiden Optisches System für Auflicht-Fluoreszenz-Mikroskopie

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DE2938056C2 (de) * 1979-09-20 1986-12-11 Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München Vorrichtung zur fluorometrischen Untersuchung von Proben
FR2502783A1 (fr) * 1981-03-25 1982-10-01 Cilas Dispositif pour mesurer l'etat d'oxydo-reduction d'un organe vivant in situ
FR2517837A1 (fr) * 1981-12-04 1983-06-10 Anvar Dispositif optimisant le couplage de deux systemes optiques pour l'observation et l'analyse d'objets
FR2525772A1 (fr) * 1982-04-27 1983-10-28 Snecma Dispositif de detection de defauts sur une piece
JPS58183863U (ja) * 1982-06-02 1983-12-07 中央ビルト工業株式会社 クレ−ン装置を有する跳出し足場
JPS5965242A (ja) * 1982-10-06 1984-04-13 Hitachi Ltd レ−ザ・ラマン・マイクロプロ−ブ
US4629687A (en) * 1982-07-29 1986-12-16 Board Of Trustees Of Michigan State University Positive selection sorting of cells
US4624915A (en) * 1982-07-29 1986-11-25 Board Of Trustees Of Michigan State University Positive selection sorting of cells
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DE3422395A1 (de) * 1983-06-16 1985-01-17 Hitachi, Ltd., Tokio/Tokyo Verfahren und vorrichtung zum ermitteln von verdrahtungsmustern
JPS6012590U (ja) * 1983-07-04 1985-01-28 ワタナベエンジニアリング株式会社 組立式の簡易搬送クレ−ン
JPS6061334U (ja) * 1983-10-04 1985-04-27 日工株式会社 小型クレ−ン
JPS60103184U (ja) * 1983-12-20 1985-07-13 越原　淳雄 小型簡易クレ−ン
JPS60124457U (ja) * 1984-01-31 1985-08-22 ナショナル住宅産業株式会社 建築用の揚重装置
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JP2520665B2 (ja) * 1987-10-16 1996-07-31 理化学研究所 蛍光顕微分光装置
FR2628530B1 (fr) * 1988-03-08 1994-01-28 Chemunex Sa Appareil et procede de detection et de numeration de particules fluorescentes, portees par un support solide
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JP2513862B2 (ja) * 1988-10-11 1996-07-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 走査型レ−ザ顕微鏡装置
JPH083481B2 (ja) * 1989-06-07 1996-01-17 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パターン読み取り装置
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US5283433A (en) * 1992-10-05 1994-02-01 The Regents Of The University Of California Scanning confocal microscope providing a continuous display
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US5646411A (en) * 1996-02-01 1997-07-08 Molecular Dynamics, Inc. Fluorescence imaging system compatible with macro and micro scanning objectives
US5847400A (en) * 1996-02-01 1998-12-08 Molecular Dynamics, Inc. Fluorescence imaging system having reduced background fluorescence
US6148114A (en) * 1996-11-27 2000-11-14 Ultrapointe Corporation Ring dilation and erosion techniques for digital image processing
US5874266A (en) * 1997-03-27 1999-02-23 Palsson; Bernhard O. Targeted system for removing tumor cells from cell populations
US6753161B2 (en) * 1997-03-27 2004-06-22 Oncosis Llc Optoinjection methods
US6534308B1 (en) * 1997-03-27 2003-03-18 Oncosis, Llc Method and apparatus for selectively targeting specific cells within a mixed cell population
JPH1196334A (ja) 1997-09-17 1999-04-09 Olympus Optical Co Ltd 画像処理装置
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WO1999060381A1 (en) * 1998-05-16 1999-11-25 The Perkin-Elmer Corporation Instrument for monitoring polymerase chain reaction of dna
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JP4270610B2 (ja) 1998-09-24 2009-06-03 オリンパス株式会社 走査型光学顕微鏡
US7423750B2 (en) * 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
US7387891B2 (en) * 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US20050279949A1 (en) 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
JP5087192B2 (ja) * 1999-11-30 2012-11-28 インテレクソン コーポレイション 細胞群内の特定細胞に選択的に照準付けする方法及び装置
US20040224421A1 (en) * 2000-06-15 2004-11-11 Deweerd Herman Bi-directional scanning method
JP2004512845A (ja) * 2000-10-24 2004-04-30 オンコシス　リミテッド　ライアビリティ　カンパニー 三次元の検体内の細胞を選択的に標的化する方法およびデバイス
US7635588B2 (en) * 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
JP3607904B2 (ja) * 2002-02-14 2005-01-05 中国電力株式会社 フジツボ類の付着防除方法
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3614359A1 (de) * 1985-07-26 1987-02-05 Jenoptik Jena Gmbh Anordnung zur bildlichen darstellung und analyse von fluoreszenzsignalen
DE3733573A1 (de) * 1987-10-03 1989-04-20 Leybold Ag Vorrichtung zum messen des fremdstoffanteils in stroemenden fluessigkeiten
DE4221063A1 (de) * 1992-06-26 1994-01-05 Thomas Dr Heiden Optisches System für Auflicht-Fluoreszenz-Mikroskopie

Also Published As

Publication number Publication date
DE2818841A1 (de) 1978-11-09
US4284897A (en) 1981-08-18
JPS53135660A (en) 1978-11-27
JPS5749888B2 (US20020051482A1-20020502-M00012.png) 1982-10-25

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Legal Events

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OAP Request for examination filed
OD Request for examination
D2 Grant after examination
8363 Opposition against the patent
8331 Complete revocation