DE2749989C3 - Züchten von verankerungsabhängigen Zellen in einer Mikroträgerkultur - Google Patents
Züchten von verankerungsabhängigen Zellen in einer MikroträgerkulturInfo
- Publication number
- DE2749989C3 DE2749989C3 DE2749989A DE2749989A DE2749989C3 DE 2749989 C3 DE2749989 C3 DE 2749989C3 DE 2749989 A DE2749989 A DE 2749989A DE 2749989 A DE2749989 A DE 2749989A DE 2749989 C3 DE2749989 C3 DE 2749989C3
- Authority
- DE
- Germany
- Prior art keywords
- microcarriers
- cells
- microcarrier
- cell
- beads
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 17
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
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- WQMAANNAZKNUDL-UHFFFAOYSA-N 2-dimethylaminoethyl chloride Chemical compound CN(C)CCCl WQMAANNAZKNUDL-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- RJURFGZVJUQBHK-UHFFFAOYSA-N actinomycin D Natural products CC1OC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C(=O)C(C(C)C)NC(=O)C1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)NC4C(=O)NC(C(N5CCCC5C(=O)N(C)CC(=O)N(C)C(C(C)C)C(=O)OC4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-UHFFFAOYSA-N 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
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- 239000011541 reaction mixture Substances 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
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- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 description 1
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- QLOKJRIVRGCVIM-UHFFFAOYSA-N 1-[(4-methylsulfanylphenyl)methyl]piperazine Chemical compound C1=CC(SC)=CC=C1CN1CCNCC1 QLOKJRIVRGCVIM-UHFFFAOYSA-N 0.000 description 1
- LVCHXPHUKPLVRQ-UHFFFAOYSA-N 2-bromo-n,n-dimethylethanamine Chemical compound CN(C)CCBr LVCHXPHUKPLVRQ-UHFFFAOYSA-N 0.000 description 1
- YMAPBXBQNXUXHG-UHFFFAOYSA-N 3-amino-3-(chloromethyl)pentane-1,5-diol Chemical compound OCCC(CCl)(N)CCO YMAPBXBQNXUXHG-UHFFFAOYSA-N 0.000 description 1
- GTNBTBUUOSXCHY-UHFFFAOYSA-N 3-amino-3-bromopentane-1,5-diol Chemical compound OCCC(Br)(N)CCO GTNBTBUUOSXCHY-UHFFFAOYSA-N 0.000 description 1
- VEDLYKFMVPKQJP-UHFFFAOYSA-N 3-amino-3-chloropentane-1,5-diol Chemical compound OCCC(Cl)(N)CCO VEDLYKFMVPKQJP-UHFFFAOYSA-N 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 108010092160 Dactinomycin Proteins 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
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- 239000004809 Teflon Substances 0.000 description 1
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- 108020005202 Viral DNA Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 1
- RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 1
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- RDHPKYGYEGBMSE-UHFFFAOYSA-N bromoethane Chemical compound CCBr RDHPKYGYEGBMSE-UHFFFAOYSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
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- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 1
- 229960000640 dactinomycin Drugs 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- SDIXRDNYIMOKSG-UHFFFAOYSA-L disodium methyl arsenate Chemical compound [Na+].[Na+].C[As]([O-])([O-])=O SDIXRDNYIMOKSG-UHFFFAOYSA-L 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
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- 238000000605 extraction Methods 0.000 description 1
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- 210000003953 foreskin Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
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- 150000003840 hydrochlorides Chemical class 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
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- 230000005764 inhibitory process Effects 0.000 description 1
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- 238000011835 investigation Methods 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 150000002734 metacrylic acid derivatives Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- BEPINZGLNNOOIJ-UHFFFAOYSA-N n-(bromomethyl)-n-ethylethanamine Chemical compound CCN(CC)CBr BEPINZGLNNOOIJ-UHFFFAOYSA-N 0.000 description 1
- ZBUQPAJRQXISTC-UHFFFAOYSA-N n-(chloromethyl)-n-ethylethanamine Chemical compound CCN(CC)CCl ZBUQPAJRQXISTC-UHFFFAOYSA-N 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 1
- 125000005496 phosphonium group Chemical group 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
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- 230000001902 propagating effect Effects 0.000 description 1
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- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000009966 trimming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74099376A | 1976-11-11 | 1976-11-11 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2749989A1 DE2749989A1 (de) | 1978-05-18 |
| DE2749989B2 DE2749989B2 (de) | 1979-10-25 |
| DE2749989C3 true DE2749989C3 (de) | 1980-07-03 |
Family
ID=24978920
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2759579A Expired DE2759579C2 (de) | 1976-11-11 | 1977-11-08 | Zellkulturmikroträger |
| DE2749989A Expired DE2749989C3 (de) | 1976-11-11 | 1977-11-08 | Züchten von verankerungsabhängigen Zellen in einer Mikroträgerkultur |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2759579A Expired DE2759579C2 (de) | 1976-11-11 | 1977-11-08 | Zellkulturmikroträger |
Country Status (14)
| Country | Link |
|---|---|
| JP (3) | JPS5362889A (show.php) |
| AU (1) | AU508398B2 (show.php) |
| BE (1) | BE860721A (show.php) |
| BR (1) | BR7707551A (show.php) |
| CA (1) | CA1063050A (show.php) |
| CH (1) | CH615947A5 (show.php) |
| DE (2) | DE2759579C2 (show.php) |
| FI (1) | FI60407C (show.php) |
| FR (1) | FR2370792A1 (show.php) |
| GB (1) | GB1535150A (show.php) |
| IN (1) | IN147612B (show.php) |
| NL (1) | NL177926C (show.php) |
| SE (2) | SE417108C (show.php) |
| ZA (1) | ZA776251B (show.php) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| FR2470794A1 (fr) * | 1979-12-05 | 1981-06-12 | Pasteur Institut | Nouvelles microparticules, leur preparation et leurs applications en biologie, notamment a la culture de cellules diploides humaines |
| US4448884A (en) * | 1982-03-03 | 1984-05-15 | Kms Fusion, Inc. | Glass-surface microcarrier for growth of cell cultures |
| JPS592735U (ja) * | 1982-06-28 | 1984-01-09 | シャープ株式会社 | 給紙用カセツト内の用紙残量指示装置 |
| JPS60126453U (ja) * | 1984-02-02 | 1985-08-26 | 富士ゼロックス株式会社 | フアクシミリ等の用紙残量表示装置 |
| IL74259A0 (en) * | 1984-02-06 | 1985-05-31 | Surface Concepts Pty Ltd | Improved method for cell culture |
| JPS60145139U (ja) * | 1984-03-07 | 1985-09-26 | 日本電信電話株式会社 | シ−ト状記録紙の残量表示装置 |
| SE454518B (sv) * | 1984-05-21 | 1988-05-09 | Statens Bakteriologiska Lab | Forfarande for odling av diploida celler i nervaro av cellulosafibrer |
| JP2606213B2 (ja) * | 1986-04-22 | 1997-04-30 | 味の素株式会社 | 修飾された微生物産生セルロースのゲルおよび動物細胞膜との複合体 |
| DE3633891A1 (de) * | 1986-10-04 | 1988-04-07 | Akzo Gmbh | Verfahren und vorrichtung zum kultivieren von tierischen zellen |
| US20140170748A1 (en) * | 2012-12-14 | 2014-06-19 | DePuy Synthes Products, LLC | Nutrient Enriched Media for hUTC Growth |
| CN113249311B (zh) * | 2020-05-25 | 2022-06-28 | 北京唐颐惠康生物医学技术有限公司 | 间充质干细胞培养用可降解微载体的制备方法及其产品和应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1436323A (en) * | 1972-04-26 | 1976-05-19 | Wellcome Found | Cell and virus culture systems |
| US3901095A (en) * | 1974-06-17 | 1975-08-26 | Joseph W Wechsler | Bicycle gear shift |
| US3935067A (en) * | 1974-11-22 | 1976-01-27 | Wyo-Ben Products, Inc. | Inorganic support for culture media |
| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| JPS5614270A (en) * | 1979-07-14 | 1981-02-12 | Ricoh Co Ltd | Wet type development after treating device |
-
1977
- 1977-10-20 ZA ZA00776251A patent/ZA776251B/xx unknown
- 1977-10-27 GB GB44659/77A patent/GB1535150A/en not_active Expired
- 1977-10-31 IN IN1561/CAL/77A patent/IN147612B/en unknown
- 1977-11-04 NL NLAANVRAGE7712202,A patent/NL177926C/xx not_active IP Right Cessation
- 1977-11-08 DE DE2759579A patent/DE2759579C2/de not_active Expired
- 1977-11-08 DE DE2749989A patent/DE2749989C3/de not_active Expired
- 1977-11-09 AU AU30491/77A patent/AU508398B2/en not_active Expired
- 1977-11-09 JP JP13365277A patent/JPS5362889A/ja active Granted
- 1977-11-10 BE BE182543A patent/BE860721A/xx not_active IP Right Cessation
- 1977-11-10 FR FR7733954A patent/FR2370792A1/fr active Granted
- 1977-11-10 CA CA290,641A patent/CA1063050A/en not_active Expired
- 1977-11-10 FI FI773377A patent/FI60407C/fi not_active IP Right Cessation
- 1977-11-10 BR BR7707551A patent/BR7707551A/pt unknown
- 1977-11-10 SE SE7712700A patent/SE417108C/xx not_active IP Right Cessation
- 1977-11-11 CH CH1382677A patent/CH615947A5/de not_active IP Right Cessation
-
1980
- 1980-10-07 SE SE8006993A patent/SE460906B/sv not_active IP Right Cessation
- 1980-10-15 JP JP14309080A patent/JPS5699790A/ja active Granted
- 1980-10-15 JP JP14308980A patent/JPS5699789A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5362889A (en) | 1978-06-05 |
| JPS5699790A (en) | 1981-08-11 |
| SE7712700L (sv) | 1978-05-12 |
| CH615947A5 (en) | 1980-02-29 |
| NL7712202A (nl) | 1978-05-16 |
| IN147612B (show.php) | 1980-05-03 |
| JPS5735953B2 (show.php) | 1982-07-31 |
| FR2370792B1 (show.php) | 1983-07-29 |
| BE860721A (nl) | 1978-03-01 |
| BR7707551A (pt) | 1978-06-20 |
| FI60407B (fi) | 1981-09-30 |
| NL177926C (nl) | 1985-12-16 |
| DE2749989A1 (de) | 1978-05-18 |
| AU3049177A (en) | 1979-06-28 |
| FI60407C (fi) | 1982-01-11 |
| JPS5699789A (en) | 1981-08-11 |
| ZA776251B (en) | 1978-07-26 |
| AU508398B2 (en) | 1980-03-20 |
| CA1063050A (en) | 1979-09-25 |
| SE417108B (sv) | 1981-02-23 |
| GB1535150A (en) | 1978-12-06 |
| NL177926B (nl) | 1985-07-16 |
| JPS5614270B2 (show.php) | 1981-04-03 |
| FR2370792A1 (fr) | 1978-06-09 |
| SE417108C (sv) | 1989-04-17 |
| DE2749989B2 (de) | 1979-10-25 |
| SE460906B (sv) | 1989-12-04 |
| FI773377A7 (fi) | 1978-05-12 |
| DE2759579C2 (de) | 1983-08-25 |
| JPS575514B2 (show.php) | 1982-01-30 |
| SE8006993L (sv) | 1980-10-07 |
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| OD | Request for examination | ||
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