GB1535150A - Cell culture microcarriers - Google Patents

Cell culture microcarriers

Info

Publication number
GB1535150A
GB1535150A GB44659/77A GB4465977A GB1535150A GB 1535150 A GB1535150 A GB 1535150A GB 44659/77 A GB44659/77 A GB 44659/77A GB 4465977 A GB4465977 A GB 4465977A GB 1535150 A GB1535150 A GB 1535150A
Authority
GB
United Kingdom
Prior art keywords
cells
microcarriers
cell culture
growth
microcarrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB44659/77A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of GB1535150A publication Critical patent/GB1535150A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A process for the cultivation of anchorage-dependent cells in which microcarriers in the cell culture are used is described. These microcarriers have on their surface an amount of positively charged chemical groups such that an exchange capacity which is in the range from 0.1 to 4.5 milliequivalents per gram of dry, untreated microcarrier is ensured. This exchange capacity ensures good growth of the cells, and the cells are cultivated in a culture medium in which the microcarrier is suspended, and the cell culture is left under conditions such that growth of the cells is elicited. The cells obtained in this cultivation process can be harvested, or by-products of the cell growth, such as, for example, viruses, vaccines, hormones, interferon or other by-products of cell growth, can be isolated. The microcarriers and process for their production are also described.
GB44659/77A 1976-11-11 1977-10-27 Cell culture microcarriers Expired GB1535150A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US74099376A 1976-11-11 1976-11-11

Publications (1)

Publication Number Publication Date
GB1535150A true GB1535150A (en) 1978-12-06

Family

ID=24978920

Family Applications (1)

Application Number Title Priority Date Filing Date
GB44659/77A Expired GB1535150A (en) 1976-11-11 1977-10-27 Cell culture microcarriers

Country Status (14)

Country Link
JP (3) JPS5362889A (en)
AU (1) AU508398B2 (en)
BE (1) BE860721A (en)
BR (1) BR7707551A (en)
CA (1) CA1063050A (en)
CH (1) CH615947A5 (en)
DE (2) DE2749989C3 (en)
FI (1) FI60407C (en)
FR (1) FR2370792A1 (en)
GB (1) GB1535150A (en)
IN (1) IN147612B (en)
NL (1) NL177926C (en)
SE (2) SE417108C (en)
ZA (1) ZA776251B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113249311A (en) * 2020-05-25 2021-08-13 北京唐颐惠康生物医学技术有限公司 Preparation method of degradable microcarrier for mesenchymal stem cell culture, product and application thereof

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
FR2470794A1 (en) * 1979-12-05 1981-06-12 Pasteur Institut NOVEL MICROPARTICLES, THEIR PREPARATION AND THEIR APPLICATIONS IN BIOLOGY, PARTICULARLY TO THE CULTURE OF HUMAN DIPLOID CELLS
US4448884A (en) * 1982-03-03 1984-05-15 Kms Fusion, Inc. Glass-surface microcarrier for growth of cell cultures
JPS592735U (en) * 1982-06-28 1984-01-09 シャープ株式会社 Remaining paper amount indicator in paper cassette
JPS60126453U (en) * 1984-02-02 1985-08-26 富士ゼロックス株式会社 Paper remaining amount display device such as facsimile
IL74259A0 (en) * 1984-02-06 1985-05-31 Surface Concepts Pty Ltd Improved method for cell culture
JPS60145139U (en) * 1984-03-07 1985-09-26 日本電信電話株式会社 Remaining amount display device for sheet recording paper
SE454518B (en) * 1984-05-21 1988-05-09 Statens Bakteriologiska Lab PROCEDURE FOR CULTURING DIPLOID CELLS IN THE PRESENTATION OF CELLULOSA FIBERS
JP2606213B2 (en) * 1986-04-22 1997-04-30 味の素株式会社 Complexes of Modified Microbial Cellulose with Gels and Animal Cell Membrane
DE3633891A1 (en) * 1986-10-04 1988-04-07 Akzo Gmbh METHOD AND DEVICE FOR CULTIVATING ANIMAL CELLS
US20140170748A1 (en) * 2012-12-14 2014-06-19 DePuy Synthes Products, LLC Nutrient Enriched Media for hUTC Growth

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1436323A (en) * 1972-04-26 1976-05-19 Wellcome Found Cell and virus culture systems
US3901095A (en) * 1974-06-17 1975-08-26 Joseph W Wechsler Bicycle gear shift
US3935067A (en) * 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
JPS5614270A (en) * 1979-07-14 1981-02-12 Ricoh Co Ltd Wet type development after treating device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113249311A (en) * 2020-05-25 2021-08-13 北京唐颐惠康生物医学技术有限公司 Preparation method of degradable microcarrier for mesenchymal stem cell culture, product and application thereof
CN113249311B (en) * 2020-05-25 2022-06-28 北京唐颐惠康生物医学技术有限公司 Preparation method of degradable microcarrier for mesenchymal stem cell culture, product and application thereof

Also Published As

Publication number Publication date
SE417108C (en) 1989-04-17
BR7707551A (en) 1978-06-20
NL177926B (en) 1985-07-16
DE2749989A1 (en) 1978-05-18
AU508398B2 (en) 1980-03-20
CH615947A5 (en) 1980-02-29
DE2749989B2 (en) 1979-10-25
NL7712202A (en) 1978-05-16
IN147612B (en) 1980-05-03
ZA776251B (en) 1978-07-26
JPS5362889A (en) 1978-06-05
FI60407C (en) 1982-01-11
AU3049177A (en) 1979-06-28
FI60407B (en) 1981-09-30
FR2370792B1 (en) 1983-07-29
JPS5699789A (en) 1981-08-11
SE7712700L (en) 1978-05-12
CA1063050A (en) 1979-09-25
DE2749989C3 (en) 1980-07-03
BE860721A (en) 1978-03-01
JPS575514B2 (en) 1982-01-30
DE2759579C2 (en) 1983-08-25
JPS5614270B2 (en) 1981-04-03
NL177926C (en) 1985-12-16
SE8006993L (en) 1980-10-07
FI773377A (en) 1978-05-12
JPS5735953B2 (en) 1982-07-31
JPS5699790A (en) 1981-08-11
SE417108B (en) 1981-02-23
FR2370792A1 (en) 1978-06-09
SE460906B (en) 1989-12-04

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19931027

728V Application for restoration filed (sect. 28/1977)
728Y Application for restoration allowed (sect. 28/1977)
PE20 Patent expired after termination of 20 years

Effective date: 19971026