DE2154277C2 - - Google Patents
Info
- Publication number
- DE2154277C2 DE2154277C2 DE2154277A DE2154277A DE2154277C2 DE 2154277 C2 DE2154277 C2 DE 2154277C2 DE 2154277 A DE2154277 A DE 2154277A DE 2154277 A DE2154277 A DE 2154277A DE 2154277 C2 DE2154277 C2 DE 2154277C2
- Authority
- DE
- Germany
- Prior art keywords
- solution
- viscosity
- dna
- parts
- extracted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 11
- 150000001447 alkali salts Chemical class 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- 230000003480 fibrinolytic effect Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 4
- 229920005989 resin Polymers 0.000 claims description 4
- 125000002091 cationic group Chemical group 0.000 claims description 3
- 239000007857 degradation product Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 102000053602 DNA Human genes 0.000 description 15
- 108020004414 DNA Proteins 0.000 description 15
- 239000000243 solution Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 159000000000 sodium salts Chemical class 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 210000001557 animal structure Anatomy 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000005686 Serum Globulins Human genes 0.000 description 1
- 108010045362 Serum Globulins Proteins 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Analytical Chemistry (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT31308/70A IT1043823B (it) | 1970-11-03 | 1970-11-03 | Procedimento per l estrazione di acidi nucleici da organi animali |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2154277A1 DE2154277A1 (de) | 1972-05-25 |
| DE2154277C2 true DE2154277C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-04-07 |
Family
ID=11233430
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712154277 Granted DE2154277A1 (de) | 1970-11-03 | 1971-10-30 | Verfahren zum teilweisen Abbau von Desoxyribonucleinsäure |
| DE19712154278 Pending DE2154278A1 (de) | 1970-11-03 | 1971-10-30 | Verfahren zur Extraktion von Alkalisalzen von Desoxyribonucleinsäure aus tierischen Organen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712154278 Pending DE2154278A1 (de) | 1970-11-03 | 1971-10-30 | Verfahren zur Extraktion von Alkalisalzen von Desoxyribonucleinsäure aus tierischen Organen |
Country Status (7)
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5967214A (ja) * | 1982-10-12 | 1984-04-16 | Kazuo Suga | 化粧品組成物 |
| EP0107161B1 (de) * | 1982-10-26 | 1989-08-09 | CTA Finanz AG | Mittel und Verfahren zur Optimierung der Gewebemasse von Organen innerhalb der genetischen Schwankungsbreite bei Menschen und Tieren |
| IT1170214B (it) * | 1983-09-12 | 1987-06-03 | Crinos Industria Farmaco | Composizione farmaceutica per la cura delle arteriopatie periferiche |
| IT1170215B (it) * | 1983-09-12 | 1987-06-03 | Crinos Industria Farmaco | Composizione farmaceutica per il trattamento di stati di insufficienza renale acuta |
| IT1206341B (it) * | 1984-02-16 | 1989-04-14 | Crinos Industria Farmaco | Composizione farmaceutica per il trattamento dell'ischemia acuta del miocardio. |
| IT1187833B (it) * | 1985-12-12 | 1987-12-23 | Farmigea Spa | Procedimento per l ottenimento di polidesossiribonucleotidi non informazionali sostanzialmente puri e dotati di attivita biologiche e prodotto relativo |
| IT1190313B (it) * | 1986-04-17 | 1988-02-16 | Crinos Industria Farmaco | Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante |
| IT1212143B (it) | 1987-06-26 | 1989-11-08 | Crinos Industria Farmaco | Composizione ad attivita'tricogena. |
| IT1222701B (it) * | 1987-09-23 | 1990-09-12 | Crinos Industria Farmaco | Composizione ad uso topico avente attivita' tricogena,antiforfora ed antiseborroica |
| IT1223322B (it) * | 1987-10-23 | 1990-09-19 | Crinos Industria Farmaco | Metodo per prevenire la formazione di coaguli sanguigni nel circuito extracorporeo di apparecchi di dialisi a composizione utile per esso |
| IT1231509B (it) * | 1989-09-07 | 1991-12-07 | Crinos Industria Farmaco | Composizione farmceutica ad uso topico per la terapia della fragilita' capillare. |
| US6699985B2 (en) * | 1991-08-21 | 2004-03-02 | Arsinur Burcoglu | Method of treating HIV infection and related secondary infections thereof |
| US5977083A (en) * | 1991-08-21 | 1999-11-02 | Burcoglu; Arsinur | Method for using polynucleotides, oligonucleotides and derivatives thereof to treat various disease states |
| IT1252174B (it) * | 1991-12-09 | 1995-06-05 | Crinos Industria Farmaco | Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento |
| CA2299119C (en) * | 1999-02-23 | 2013-02-05 | Qiagen Gmbh | A method of stabilizing and/or isolating nucleic acids |
| ATE311907T1 (de) | 1999-06-08 | 2005-12-15 | Gentium Spa | Anwendung von komplexen von kationischen liposomen und polydeoxyribonukleotiden wie arzneimitteln |
| US6323743B1 (en) * | 1999-08-24 | 2001-11-27 | Tresness Irrevocable Patent Trust | Electronic filter assembly |
| US8771663B2 (en) * | 2000-04-18 | 2014-07-08 | Gentium Spa | Formulation having mobilising activity |
| EP1147777A1 (en) * | 2000-04-18 | 2001-10-24 | Crinos Industria Farmacobiologica S.p.A. | Combination of defibrotide and G-CSF and its use to activate haematopoietic progenitors |
| ITMI20031714A1 (it) * | 2003-09-05 | 2005-03-06 | Gentium Spa | Formazioni ad azione antitumorale. |
| MX2007010754A (es) * | 2005-03-03 | 2007-11-07 | Gentium Spa | Defibrotide y/u oligodeoxiribonucleotidos para tratar tumores dependientes de angiogenesis. |
| EP1872787A1 (en) * | 2006-06-27 | 2008-01-02 | Gentium S.p.A. | Use of defibrotide for the inhibition of heparanase |
| EP1982722A1 (en) * | 2007-04-16 | 2008-10-22 | Gentium S.p.A. | Use of oligotide for the treatment of renal diseases |
| EP2103689A1 (en) * | 2008-03-19 | 2009-09-23 | Gentium S.p.A. | Synthetic phosphodiester oligonucleotides and therapeutical uses thereof |
| CN107007617A (zh) | 2010-11-12 | 2017-08-04 | 真蒂奥姆责任有限公司 | 去纤维蛋白多核苷酸用于预防和/或治疗移植物抗宿主病(gvhd) |
| KR20190112197A (ko) | 2012-06-22 | 2019-10-02 | 젠티엄 에스알엘 | 디파이브로타이드의 생물학적 활성 측정을 위한 유우글로불린에 기초한 방법 |
| EP3026122A1 (en) | 2014-11-27 | 2016-06-01 | Gentium S.p.A. | Cellular-based method for determining the potency of defibrotide |
| SG11202000952PA (en) | 2017-08-03 | 2020-02-27 | Jazz Pharmaceuticals Ireland Ltd | Formulations comprising a nucleic acid in a high concentration |
| AU2019252790A1 (en) | 2018-04-12 | 2020-10-15 | Jazz Pharmaceuticals, Inc. | Defibrotide for the prevention and treatment of cytokine release syndrome and neurotoxicity associated with immunodepletion |
| US20220023533A1 (en) | 2018-12-07 | 2022-01-27 | Jazz Phrmaceticals Ireland Limited | Subcutaneous delivery of high concentration formulations |
| EP4110287A1 (en) | 2020-02-28 | 2023-01-04 | Jazz Pharmaceuticals Ireland Limited | Delivery of low viscosity formulations |
| US20230086833A1 (en) | 2020-04-17 | 2023-03-23 | Jazz Pharmaceuticals Ireland Limited | Defibrotide treatment for the prevention of organ rejection and injury |
| WO2022234101A1 (en) | 2021-05-06 | 2022-11-10 | Jazz Pharmaceuticals Ireland Limited | Defibrotide for the treatment and prevention of acute respiratory distress syndrome |
-
1970
- 1970-11-03 IT IT31308/70A patent/IT1043823B/it active
-
1971
- 1971-10-29 SE SE7113831A patent/SE385376B/xx unknown
- 1971-10-29 SE SE7113830A patent/SE385902B/xx unknown
- 1971-10-30 DE DE19712154277 patent/DE2154277A1/de active Granted
- 1971-10-30 DE DE19712154278 patent/DE2154278A1/de active Pending
- 1971-11-02 FR FR7139256A patent/FR2131248A5/fr not_active Expired
- 1971-11-02 US US00194919A patent/US3770720A/en not_active Expired - Lifetime
- 1971-11-02 FR FR7139257A patent/FR2131249A5/fr not_active Expired
- 1971-11-03 GB GB5104771A patent/GB1366647A/en not_active Expired
- 1971-11-03 GB GB5104871A patent/GB1367656A/en not_active Expired
- 1971-11-04 JP JP46087235A patent/JPS5229320B1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| GB1367656A (en) | 1974-09-18 |
| JPS5229320B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-08-01 |
| US3770720A (en) | 1973-11-06 |
| DE2154278A1 (de) | 1972-05-25 |
| FR2131249A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-11-10 |
| GB1366647A (en) | 1974-09-11 |
| SE385902B (sv) | 1976-07-26 |
| SE385376B (sv) | 1976-06-28 |
| FR2131248A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-11-10 |
| DE2154277A1 (de) | 1972-05-25 |
| IT1043823B (it) | 1980-02-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8127 | New person/name/address of the applicant |
Owner name: CRINOS INDUSTRIA FARMACOBIOLOGIA S.P.A., VILLA GUA |
|
| 8128 | New person/name/address of the agent |
Representative=s name: SCHOENWALD, K., DR.-ING. FUES, J., DIPL.-CHEM. DR. |
|
| 8125 | Change of the main classification |
Ipc: A61K 31/70 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition |