DE2153479C3 - - Google Patents
Info
- Publication number
- DE2153479C3 DE2153479C3 DE19712153479 DE2153479A DE2153479C3 DE 2153479 C3 DE2153479 C3 DE 2153479C3 DE 19712153479 DE19712153479 DE 19712153479 DE 2153479 A DE2153479 A DE 2153479A DE 2153479 C3 DE2153479 C3 DE 2153479C3
- Authority
- DE
- Germany
- Prior art keywords
- white blood
- solution
- blood cells
- cells
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 claims description 50
- 210000000265 leukocyte Anatomy 0.000 claims description 49
- 210000004027 cell Anatomy 0.000 claims description 46
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 28
- 239000000758 substrate Substances 0.000 claims description 26
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims description 21
- 210000003979 eosinophil Anatomy 0.000 claims description 21
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
- 229940088598 enzyme Drugs 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 16
- 239000002244 precipitate Substances 0.000 claims description 16
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 15
- 210000000440 neutrophil Anatomy 0.000 claims description 15
- 230000000093 cytochemical effect Effects 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 13
- 239000008280 blood Substances 0.000 claims description 13
- 102000003992 Peroxidases Human genes 0.000 claims description 12
- 210000003743 erythrocyte Anatomy 0.000 claims description 12
- 210000001616 monocyte Anatomy 0.000 claims description 12
- 238000010186 staining Methods 0.000 claims description 11
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 claims description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 10
- 206010018910 Haemolysis Diseases 0.000 claims description 9
- 230000008878 coupling Effects 0.000 claims description 9
- 238000010168 coupling process Methods 0.000 claims description 9
- 238000005859 coupling reaction Methods 0.000 claims description 9
- 230000003197 catalytic effect Effects 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- -1 Naphthol ester Chemical class 0.000 claims description 7
- 210000000601 blood cell Anatomy 0.000 claims description 7
- 239000000834 fixative Substances 0.000 claims description 7
- 230000002949 hemolytic effect Effects 0.000 claims description 7
- 239000006174 pH buffer Substances 0.000 claims description 7
- 150000002978 peroxides Chemical class 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 230000002380 cytological effect Effects 0.000 claims description 5
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 claims description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 4
- 229960002897 heparin Drugs 0.000 claims description 4
- 229920000669 heparin Polymers 0.000 claims description 4
- XIVJNRXPRQKFRZ-UHFFFAOYSA-N naphthalen-1-yl butanoate Chemical compound C1=CC=C2C(OC(=O)CCC)=CC=CC2=C1 XIVJNRXPRQKFRZ-UHFFFAOYSA-N 0.000 claims description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-M 1-naphthaleneacetate Chemical compound C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-M 0.000 claims description 3
- 108090001060 Lipase Proteins 0.000 claims description 3
- PCKPVGOLPKLUHR-UHFFFAOYSA-N OH-Indolxyl Natural products C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 claims description 3
- 238000004040 coloring Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 claims description 2
- 102000004882 Lipase Human genes 0.000 claims description 2
- 239000004367 Lipase Substances 0.000 claims description 2
- 108010023197 Streptokinase Proteins 0.000 claims description 2
- 230000002159 abnormal effect Effects 0.000 claims description 2
- 125000001931 aliphatic group Chemical group 0.000 claims description 2
- 238000006555 catalytic reaction Methods 0.000 claims description 2
- 239000012954 diazonium Substances 0.000 claims description 2
- 150000001989 diazonium salts Chemical class 0.000 claims description 2
- 239000008098 formaldehyde solution Substances 0.000 claims description 2
- 230000001744 histochemical effect Effects 0.000 claims description 2
- 235000019421 lipase Nutrition 0.000 claims description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 2
- 229960005202 streptokinase Drugs 0.000 claims description 2
- 230000004069 differentiation Effects 0.000 claims 7
- ZTQSAGDEMFDKMZ-UHFFFAOYSA-N Butyraldehyde Chemical compound CCCC=O ZTQSAGDEMFDKMZ-UHFFFAOYSA-N 0.000 claims 3
- NBBJYMSMWIIQGU-UHFFFAOYSA-N Propionic aldehyde Chemical compound CCC=O NBBJYMSMWIIQGU-UHFFFAOYSA-N 0.000 claims 2
- 229940079593 drug Drugs 0.000 claims 2
- 239000003814 drug Substances 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 108090000371 Esterases Proteins 0.000 claims 1
- 108091005461 Nucleic proteins Proteins 0.000 claims 1
- 210000003651 basophil Anatomy 0.000 claims 1
- 238000004820 blood count Methods 0.000 claims 1
- 210000004556 brain Anatomy 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 230000000415 inactivating effect Effects 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 239000008399 tap water Substances 0.000 claims 1
- 235000020679 tap water Nutrition 0.000 claims 1
- HGBOYTHUEUWSSQ-UHFFFAOYSA-N valeric aldehyde Natural products CCCCC=O HGBOYTHUEUWSSQ-UHFFFAOYSA-N 0.000 claims 1
- 239000000243 solution Substances 0.000 description 31
- 239000011550 stock solution Substances 0.000 description 12
- 239000000975 dye Substances 0.000 description 9
- 230000008588 hemolysis Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 239000000460 chlorine Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 102000001554 Hemoglobins Human genes 0.000 description 4
- 108010054147 Hemoglobins Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 4
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 4
- 239000012192 staining solution Substances 0.000 description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 3
- 108010053835 Catalase Proteins 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 241000270295 Serpentes Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 3
- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 2
- JFGQHAHJWJBOPD-UHFFFAOYSA-N 3-hydroxy-n-phenylnaphthalene-2-carboxamide Chemical class OC1=CC2=CC=CC=C2C=C1C(=O)NC1=CC=CC=C1 JFGQHAHJWJBOPD-UHFFFAOYSA-N 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 229940122601 Esterase inhibitor Drugs 0.000 description 2
- JBOPQACSHPPKEP-UHFFFAOYSA-N Indoxyl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CNC2=C1 JBOPQACSHPPKEP-UHFFFAOYSA-N 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229940052223 basic fuchsin Drugs 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000002329 esterase inhibitor Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000010288 sodium nitrite Nutrition 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
- RRQYJINTUHWNHW-UHFFFAOYSA-N 1-ethoxy-2-(2-ethoxyethoxy)ethane Chemical compound CCOCCOCCOCC RRQYJINTUHWNHW-UHFFFAOYSA-N 0.000 description 1
- DPUSLOQBAPOARC-UHFFFAOYSA-N 1h-indol-3-yl butanoate Chemical compound C1=CC=C2C(OC(=O)CCC)=CNC2=C1 DPUSLOQBAPOARC-UHFFFAOYSA-N 0.000 description 1
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- 235000014443 Pyrus communis Nutrition 0.000 description 1
- CVJGNNYDVQYHEO-UHFFFAOYSA-N [3-(phenylcarbamoyl)naphthalen-2-yl] acetate Chemical compound CC(=O)OC1=CC2=CC=CC=C2C=C1C(=O)NC1=CC=CC=C1 CVJGNNYDVQYHEO-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 229940089960 chloroacetate Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005112 continuous flow technique Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000009897 hydrogen peroxide bleaching Methods 0.000 description 1
- ILHIHKRJJMKBEE-UHFFFAOYSA-N hydroperoxyethane Chemical compound CCOO ILHIHKRJJMKBEE-UHFFFAOYSA-N 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 150000001451 organic peroxides Chemical class 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229960001922 sodium perborate Drugs 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- YKLJGMBLPUQQOI-UHFFFAOYSA-M sodium;oxidooxy(oxo)borane Chemical compound [Na+].[O-]OB=O YKLJGMBLPUQQOI-UHFFFAOYSA-M 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- MSLRPWGRFCKNIZ-UHFFFAOYSA-J tetrasodium;hydrogen peroxide;dicarbonate Chemical compound [Na+].[Na+].[Na+].[Na+].OO.OO.OO.[O-]C([O-])=O.[O-]C([O-])=O MSLRPWGRFCKNIZ-UHFFFAOYSA-J 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical compound [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8533370A | 1970-10-30 | 1970-10-30 | |
| US8533370 | 1970-10-30 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DE2153479A1 DE2153479A1 (de) | 1972-05-04 |
| DE2153479B2 DE2153479B2 (de) | 1975-06-05 |
| DE2153479C3 true DE2153479C3 (enExample) | 1976-01-22 |
Family
ID=22190904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2153479A Granted DE2153479B2 (de) | 1970-10-30 | 1971-10-27 | Verfahren zum Nachweisen einer weißen Blutzellenart |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US3741875A (enExample) |
| JP (2) | JPS576932B1 (enExample) |
| AU (1) | AU460469B2 (enExample) |
| BE (1) | BE774311A (enExample) |
| CA (1) | CA960947A (enExample) |
| DE (1) | DE2153479B2 (enExample) |
| FR (1) | FR2113336A5 (enExample) |
| GB (1) | GB1331568A (enExample) |
| IT (1) | IT961518B (enExample) |
| NL (1) | NL172463C (enExample) |
| SE (1) | SE372417B (enExample) |
| SU (1) | SU611598A3 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2125699B2 (de) * | 1971-05-25 | 1973-05-17 | Phywe AG, 3400 Gottingen | Praeparation von zellen zur messung von dns |
| US4049381A (en) * | 1976-03-23 | 1977-09-20 | Technicon Instruments Corporation | Apparatus and method of fluid sample analysis |
| US4099917A (en) * | 1977-07-21 | 1978-07-11 | Technicon Instruments Corporation | Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles |
| DE2826965A1 (de) * | 1978-06-20 | 1980-01-17 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten und dafuer geeignete chromogene |
| US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
| US4492752A (en) * | 1982-09-03 | 1985-01-08 | Ortho Diagnostics Systems Inc. | Method for discriminating between unstained and absorbing dye stained cells |
| CA1254134A (en) * | 1984-03-28 | 1989-05-16 | Mount Sinai School Of Medicine Of The City University Of New York | Specific binding flow cytometry method |
| US4654312A (en) * | 1984-05-14 | 1987-03-31 | Becton, Dickinson And Company | Lysing agent for analysis of peripheral blood cells |
| US4751179A (en) * | 1984-05-31 | 1988-06-14 | Coulter Electronics, Inc. | Method and reagents for differential determination of four populations of leukocytes in blood |
| US4801549A (en) * | 1985-09-06 | 1989-01-31 | Technicon Instruments Corporation | Method for the determination of a differential white blood cell count |
| AU602129B2 (en) * | 1985-09-06 | 1990-10-04 | Technicon Instruments Corportion | Method for the determination of a differential white blood cell count |
| US4978624A (en) * | 1985-09-06 | 1990-12-18 | Technicon Instruments Corporation | Reagent for the determination of a differential white blood cell count |
| US5389549A (en) * | 1987-05-29 | 1995-02-14 | Toa Medical Electronics Co., Ltd. | Method for classifying leukocytes and a reagent used therefor |
| WO1989007880A2 (en) * | 1988-02-10 | 1989-09-08 | Nygene Corporation | Process for producing biochemicals |
| US5316725A (en) * | 1991-06-07 | 1994-05-31 | Edward Lawrence Carver, Jr. | Reagent system for the improved determination of white blood cell subpopulations |
| US5262329A (en) * | 1991-06-13 | 1993-11-16 | Carver Jr Edward L | Method for improved multiple species blood analysis |
| US6046019A (en) * | 1991-07-09 | 2000-04-04 | Goumeniouk; Alexander P. | Diagnostic kits and methods for making granulocyte cell counts |
| JP3359921B2 (ja) * | 1992-02-24 | 2002-12-24 | クールター インターナショナル コーポレイション | 血液組成物用懸濁媒体及びその使用方法 |
| US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
| US6362003B1 (en) | 1992-02-24 | 2002-03-26 | Coulter Corporation | Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof |
| JP3391451B2 (ja) * | 1992-02-24 | 2003-03-31 | クールター インターナショナル コーポレイション | 白血球類縁体用の血液対照組成物，およびその調製方法および使用方法 |
| US6812032B1 (en) * | 1993-01-21 | 2004-11-02 | Cdc Technologies, Inc. | Apparatus and method for making a plurality of reagent mixtures and analyzing particle distributions of the reagent mixtures |
| US5599501A (en) * | 1994-11-10 | 1997-02-04 | Ciba Corning Diagnostics Corp. | Incubation chamber |
| WO1996034283A1 (en) * | 1995-04-27 | 1996-10-31 | Hematronix, Inc. | Lytic system utilizing propionic acid for leukocytes differentiation |
| US5639630A (en) * | 1995-05-16 | 1997-06-17 | Bayer Corporation | Method and reagent composition for performing leukocyte differential counts on fresh and aged whole blood samples, based on intrinsic peroxidase activity of leukocytes |
| US5843608A (en) * | 1995-06-08 | 1998-12-01 | Coulter International Corp. | Reagent and method for differential determination of leukocytes in blood |
| US5686308A (en) * | 1995-06-08 | 1997-11-11 | Coulter Corporation | Reagent and method for differential determination of leukocytes in blood |
| US6146901A (en) * | 1997-06-16 | 2000-11-14 | Hematronix, Inc. | Composition for manipulating optical and electrical properties of particles to achieve target values for such properties and methods for using the composition |
| US6759246B1 (en) | 2001-11-30 | 2004-07-06 | Research & Diagnostic Systems, Inc. | Hematology control composition including lymphocyte analogs and method for preparation and use |
| CA2428740A1 (en) | 2002-05-20 | 2003-11-20 | Bayer Corporation | Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf) |
| EP2525222A1 (en) | 2011-05-17 | 2012-11-21 | Markus M. Heiss | Initial relative lymphocyte count as predictive biomarker |
| JP6933712B2 (ja) | 2016-09-27 | 2021-09-08 | ノベリス・インコーポレイテッドＮｏｖｅｌｉｓ Ｉｎｃ． | 表面品質を制御する金属の磁気浮上加熱 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2807416A (en) * | 1953-07-13 | 1957-09-24 | Ohio Commw Eng Co | Device for automatically counting blood cells |
| DE1200577B (de) * | 1962-05-29 | 1965-09-09 | Habil Hans Meyer Doering Dr Me | Anordnung zum Zaehlen der in einem stroemungs-faehigen und lichtdurchlaessigen Medium mitgefuehrten, die Helligkeit eines auf sie gerichteten Lichtstrahles beeinflussenden Teilchen |
| US3413464A (en) * | 1965-04-29 | 1968-11-26 | Ibm | Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution |
| US3523733A (en) * | 1966-01-05 | 1970-08-11 | Technicon Corp | Method and apparatus for particle counting |
| US3427135A (en) * | 1966-07-11 | 1969-02-11 | Technicon Instr | Hematology apparatus |
-
1970
- 1970-10-30 US US00085333A patent/US3741875A/en not_active Expired - Lifetime
-
1971
- 1971-10-21 CA CA125,753A patent/CA960947A/en not_active Expired
- 1971-10-21 AU AU34847/71A patent/AU460469B2/en not_active Expired
- 1971-10-22 BE BE774311A patent/BE774311A/xx not_active IP Right Cessation
- 1971-10-26 GB GB4971771A patent/GB1331568A/en not_active Expired
- 1971-10-27 SE SE7113658A patent/SE372417B/xx unknown
- 1971-10-27 DE DE2153479A patent/DE2153479B2/de active Granted
- 1971-10-28 IT IT30496/71A patent/IT961518B/it active
- 1971-10-28 FR FR7138709A patent/FR2113336A5/fr not_active Expired
- 1971-10-29 SU SU711711809A patent/SU611598A3/ru active
- 1971-10-29 JP JP8567171A patent/JPS576932B1/ja active Pending
- 1971-11-01 NL NLAANVRAGE7114995,A patent/NL172463C/xx not_active IP Right Cessation
-
1980
- 1980-07-03 JP JP9002780A patent/JPS56163455A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE2153479C3 (enExample) | ||
| DE2153479B2 (de) | Verfahren zum Nachweisen einer weißen Blutzellenart | |
| DE3587036T2 (de) | Verfahren und reagenziensystem zur differentiellen bestimmung vierer populationen von leukozyten. | |
| DE69434942T2 (de) | Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben | |
| DE3854983T2 (de) | System zur isolierung und identifizierung von leukozyten | |
| DE2823916C2 (enExample) | ||
| DE3586159T2 (de) | Zusammensetzung und verfahren zur differenzierung von weissen blutzellen. | |
| DE69221086T2 (de) | Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen | |
| DE69211434T2 (de) | Verfahren zur Probevorbereitung zur Klassifizierung und Zählung der Leukozyten | |
| DE3784610T2 (de) | Verfahren zur bestimmung von 1,5-anhydroglucitol und ausruestung dafuer. | |
| DE3587636T2 (de) | Reagenssystem und verfahren zum nachweis, aufzählen und prüfen der klassen und unterklassen von blutleukozyten. | |
| DE3021259A1 (de) | Mit amidopyrin verbessertes trinder-reagens und verfahren zur quantitativen bestimmung mit diesem reagens des wasserstoffperoxids zur enzymatischen oxidation von stoffwechselprodukten | |
| DE3689218T2 (de) | Verfahren zur differenzierten Bestimmung weisser Blutkörperchen. | |
| Evenson et al. | Flow cytometric evaluation of sperm from patients with testicular carcinoma | |
| DE4333805A1 (de) | Verfahren zur Extraktion von Nucleinsäuren und Verfahren zum Nachweis spezifizierter Nucleinsäuresequenzen | |
| EP0664454B1 (de) | Verfahren und Reagenz zur Bestimmung von Eisen | |
| Sorokin et al. | Macrophage development: I. Rationale for using Griffonia simplicifolia isolectin B4 as a marker for the line | |
| Roels et al. | Light microscopic visualization of peroxisomes and plasmalogens in first trimester chorionic villi | |
| DE2759962C2 (de) | Verfahren zur quantitativen Bestimmung von Harnstoff | |
| DE69014512T2 (de) | Reagens zum Nachweis von Bilirubin in Urin. | |
| EP0952453B1 (de) | Verfahren und Reagenz zur störungsfreien Bestimmung von Eisen | |
| DE4321807C2 (de) | Verfahren zur quantitativen Bestimmung von 1,5-Anhydroglucitol und Testpack | |
| DE69332764T2 (de) | Hydroxylamin enthaltendes kontrollreagenz | |
| DE69128873T2 (de) | Verfahren zur enzymatischen Messung von Wasserstoffperoxid und Reagenz dafür | |
| DE102005044530B4 (de) | Verfahren zur geschlechtsspezifischen Selektion von Säugerspermatozoen |