DE2007792A1 - Verfahren zur Herstellung einer L-Glutaminase - Google Patents
Verfahren zur Herstellung einer L-GlutaminaseInfo
- Publication number
- DE2007792A1 DE2007792A1 DE19702007792 DE2007792A DE2007792A1 DE 2007792 A1 DE2007792 A1 DE 2007792A1 DE 19702007792 DE19702007792 DE 19702007792 DE 2007792 A DE2007792 A DE 2007792A DE 2007792 A1 DE2007792 A1 DE 2007792A1
- Authority
- DE
- Germany
- Prior art keywords
- glutaminase
- pseudomonas aureofaciens
- enzyme
- pseudomonas
- acetone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102100025961 Glutaminase liver isoform, mitochondrial Human genes 0.000 title claims description 51
- 101710138819 Glutaminase liver isoform, mitochondrial Proteins 0.000 title claims description 51
- 238000000034 method Methods 0.000 title claims description 13
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 34
- 241001645955 Pseudomonas chlororaphis subsp. aureofaciens Species 0.000 claims description 24
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 108090000790 Enzymes Proteins 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 206010028980 Neoplasm Diseases 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
- 239000000243 solution Substances 0.000 description 22
- 229940088598 enzyme Drugs 0.000 description 19
- 210000004027 cell Anatomy 0.000 description 18
- 102000009127 Glutaminase Human genes 0.000 description 13
- 108010073324 Glutaminase Proteins 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 238000001556 precipitation Methods 0.000 description 13
- 238000005194 fractionation Methods 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000000463 material Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- 239000010414 supernatant solution Substances 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 7
- 229920001223 polyethylene glycol Polymers 0.000 description 7
- 241000589516 Pseudomonas Species 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000013049 sediment Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000002202 Polyethylene glycol Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 4
- 229930182816 L-glutamine Natural products 0.000 description 4
- 239000007979 citrate buffer Substances 0.000 description 4
- 238000005352 clarification Methods 0.000 description 4
- 150000002009 diols Chemical class 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- ACOQOLIJGGKILA-UHFFFAOYSA-N 2-methylpentane-1,1-diol Chemical compound CCCC(C)C(O)O ACOQOLIJGGKILA-UHFFFAOYSA-N 0.000 description 3
- JSGVZVOGOQILFM-UHFFFAOYSA-N 3-methoxy-1-butanol Chemical compound COC(C)CCO JSGVZVOGOQILFM-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
- 241000193403 Clostridium Species 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229960002989 glutamic acid Drugs 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229920000570 polyether Polymers 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 102100025573 1-alkyl-2-acetylglycerophosphocholine esterase Human genes 0.000 description 1
- KNIUHBNRWZGIQQ-UHFFFAOYSA-N 7-diethoxyphosphinothioyloxy-4-methylchromen-2-one Chemical compound CC1=CC(=O)OC2=CC(OP(=S)(OCC)OCC)=CC=C21 KNIUHBNRWZGIQQ-UHFFFAOYSA-N 0.000 description 1
- 108010024976 Asparaginase Proteins 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 241000023320 Luma <angiosperm> Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical class CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003918 fraction a Anatomy 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 description 1
- -1 hydrogen ions Chemical class 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 238000007693 zone electrophoresis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19702007792 DE2007792A1 (de) | 1970-02-20 | 1970-02-20 | Verfahren zur Herstellung einer L-Glutaminase |
| IL36109A IL36109A0 (en) | 1970-02-20 | 1971-02-01 | Process for the production of an l-glutaminase |
| AU25277/71A AU2527771A (en) | 1970-02-20 | 1971-02-10 | Process forthe production ofan 1-glutaminase |
| SU1620861A SU371704A1 (ru) | 1971-02-12 | Способ получения l-глютаминазы | |
| NL7102059A NL7102059A (enrdf_load_stackoverflow) | 1970-02-20 | 1971-02-16 | |
| AT135471A AT299858B (de) | 1970-02-20 | 1971-02-17 | Verfahren zur Herstellung von gegebenenfalls kristallisierter L-Glutaminase |
| BE763215A BE763215A (fr) | 1970-02-20 | 1971-02-19 | Procede de preparation d'une l-glutaminase |
| FR7105814A FR2081530A1 (enrdf_load_stackoverflow) | 1970-02-20 | 1971-02-19 | |
| HUBA2540A HU162200B (enrdf_load_stackoverflow) | 1970-02-20 | 1971-02-19 | |
| GB1296625D GB1296625A (enrdf_load_stackoverflow) | 1970-02-20 | 1971-04-19 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19702007792 DE2007792A1 (de) | 1970-02-20 | 1970-02-20 | Verfahren zur Herstellung einer L-Glutaminase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2007792A1 true DE2007792A1 (de) | 1971-12-23 |
Family
ID=5762799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19702007792 Pending DE2007792A1 (de) | 1970-02-20 | 1970-02-20 | Verfahren zur Herstellung einer L-Glutaminase |
Country Status (9)
| Country | Link |
|---|---|
| AT (1) | AT299858B (enrdf_load_stackoverflow) |
| AU (1) | AU2527771A (enrdf_load_stackoverflow) |
| BE (1) | BE763215A (enrdf_load_stackoverflow) |
| DE (1) | DE2007792A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2081530A1 (enrdf_load_stackoverflow) |
| GB (1) | GB1296625A (enrdf_load_stackoverflow) |
| HU (1) | HU162200B (enrdf_load_stackoverflow) |
| IL (1) | IL36109A0 (enrdf_load_stackoverflow) |
| NL (1) | NL7102059A (enrdf_load_stackoverflow) |
-
1970
- 1970-02-20 DE DE19702007792 patent/DE2007792A1/de active Pending
-
1971
- 1971-02-01 IL IL36109A patent/IL36109A0/xx unknown
- 1971-02-10 AU AU25277/71A patent/AU2527771A/en not_active Expired
- 1971-02-16 NL NL7102059A patent/NL7102059A/xx unknown
- 1971-02-17 AT AT135471A patent/AT299858B/de not_active IP Right Cessation
- 1971-02-19 HU HUBA2540A patent/HU162200B/hu unknown
- 1971-02-19 BE BE763215A patent/BE763215A/xx unknown
- 1971-02-19 FR FR7105814A patent/FR2081530A1/fr not_active Withdrawn
- 1971-04-19 GB GB1296625D patent/GB1296625A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NL7102059A (enrdf_load_stackoverflow) | 1971-08-24 |
| AU2527771A (en) | 1972-08-17 |
| AT299858B (de) | 1972-07-10 |
| FR2081530A1 (enrdf_load_stackoverflow) | 1971-12-03 |
| HU162200B (enrdf_load_stackoverflow) | 1973-01-29 |
| SU371704A3 (enrdf_load_stackoverflow) | 1973-02-22 |
| BE763215A (fr) | 1971-08-19 |
| GB1296625A (enrdf_load_stackoverflow) | 1972-11-15 |
| IL36109A0 (en) | 1971-04-28 |
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