DE19746874A1 - Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen - Google Patents

Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen

Info

Publication number
DE19746874A1
DE19746874A1 DE19746874A DE19746874A DE19746874A1 DE 19746874 A1 DE19746874 A1 DE 19746874A1 DE 19746874 A DE19746874 A DE 19746874A DE 19746874 A DE19746874 A DE 19746874A DE 19746874 A1 DE19746874 A1 DE 19746874A1
Authority
DE
Germany
Prior art keywords
nucleic acids
membrane
acid
immobilize
salt
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE19746874A
Other languages
German (de)
English (en)
Inventor
Helge Dr Bastian
Simone Gauch
Uwe Dr Oelmueller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to DE19746874A priority Critical patent/DE19746874A1/de
Priority to AT98955519T priority patent/ATE335846T1/de
Priority to PCT/EP1998/006756 priority patent/WO1999022021A1/de
Priority to DE59813680T priority patent/DE59813680D1/de
Priority to AU12318/99A priority patent/AU1231899A/en
Priority to EP98955519A priority patent/EP1049801B1/de
Priority to JP2000518111A priority patent/JP4440458B2/ja
Priority to PCT/EP1999/002664 priority patent/WO2000024927A1/de
Priority to EP10007053A priority patent/EP2251440A3/de
Priority to EP99923428A priority patent/EP1121460B1/de
Publication of DE19746874A1 publication Critical patent/DE19746874A1/de
Priority to US09/536,736 priority patent/US8008475B1/en
Priority to US10/300,111 priority patent/US20030152974A1/en
Priority to US11/155,811 priority patent/US20050244882A1/en
Priority to JP2008101050A priority patent/JP2008263978A/ja
Priority to JP2008101055A priority patent/JP2008245649A/ja
Priority to US12/584,754 priority patent/US20100021925A1/en
Priority to US12/584,772 priority patent/US20100159460A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Detergent Compositions (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Cleaning Or Drying Semiconductors (AREA)
  • Saccharide Compounds (AREA)
DE19746874A 1997-10-23 1997-10-23 Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen Ceased DE19746874A1 (de)

Priority Applications (17)

Application Number Priority Date Filing Date Title
DE19746874A DE19746874A1 (de) 1997-10-23 1997-10-23 Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
AT98955519T ATE335846T1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen
PCT/EP1998/006756 WO1999022021A1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen
DE59813680T DE59813680D1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen
AU12318/99A AU1231899A (en) 1997-10-23 1998-10-23 Method for isolating and purifying nucleic acids on surfaces
EP98955519A EP1049801B1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen
JP2000518111A JP4440458B2 (ja) 1997-10-23 1998-10-23 表面上の核酸類の単離及び精製のための方法
EP99923428A EP1121460B1 (de) 1997-10-23 1999-04-20 Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen
EP10007053A EP2251440A3 (de) 1997-10-23 1999-04-20 Verfahren und Mittel zur Isolierung und Reinigung von Nukleinsäuren an Oberflächen
PCT/EP1999/002664 WO2000024927A1 (de) 1997-10-23 1999-04-20 Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen
US09/536,736 US8008475B1 (en) 1997-10-23 2000-03-28 Method for isolating and purifying nucleic acids on surfaces
US10/300,111 US20030152974A1 (en) 1997-10-23 2002-11-20 Isolation of nucleic acids on surfaces
US11/155,811 US20050244882A1 (en) 1997-10-23 2005-06-17 Isolation of nucleic acids on surfaces
JP2008101050A JP2008263978A (ja) 1997-10-23 2008-04-09 表面における核酸の単離及び精製のための方法並びに手段
JP2008101055A JP2008245649A (ja) 1997-10-23 2008-04-09 表面における核酸の単離及び精製のための方法並びに手段
US12/584,754 US20100021925A1 (en) 1997-10-23 2009-09-11 Isolation of nucleic acids on surfaces
US12/584,772 US20100159460A1 (en) 1997-10-23 2009-09-11 Isolation of nucleic acids on surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19746874A DE19746874A1 (de) 1997-10-23 1997-10-23 Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
EP99923428A EP1121460B1 (de) 1997-10-23 1999-04-20 Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen

Publications (1)

Publication Number Publication Date
DE19746874A1 true DE19746874A1 (de) 1999-04-29

Family

ID=85514527

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19746874A Ceased DE19746874A1 (de) 1997-10-23 1997-10-23 Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
DE59813680T Expired - Lifetime DE59813680D1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE59813680T Expired - Lifetime DE59813680D1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen

Country Status (7)

Country Link
US (5) US8008475B1 (https=)
EP (3) EP1049801B1 (https=)
JP (3) JP4440458B2 (https=)
AT (1) ATE335846T1 (https=)
AU (1) AU1231899A (https=)
DE (2) DE19746874A1 (https=)
WO (2) WO1999022021A1 (https=)

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WO2001009389A3 (en) * 1999-08-02 2001-08-16 Molecular Dynamics Inc Methods and apparatus for template capture and normalization for submicroliter reaction
WO2001059098A3 (de) * 2000-02-11 2002-05-23 Eppendorf Ag Verfahren zur aufreinigung von nukleinsäuren
EP1256627A3 (de) * 2001-05-11 2003-06-04 QIAGEN GmbH Verwendung von Alkanen zur kontaminationsfreien Reinigung bzw. Trennung von Biopolymeren
WO2005003346A1 (en) * 2003-06-06 2005-01-13 Applera Corporation Purification device for ribonucleic acid in large volumes, and method
EP1526176A3 (en) * 2003-10-24 2005-05-11 Agilent Technologies Inc. (a Delaware Corporation) Devices and methods for isolating RNA
EP1548126A1 (en) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Solid support for control nucleic acid, and application thereof to nucleic acid detection
WO2008039668A1 (en) * 2006-09-26 2008-04-03 Ge Healthcare Bio-Sciences Corp. Nucleic acid purification method
EP2025751A1 (en) 2007-08-13 2009-02-18 Taigen Bioscience Corporation Method for washing a column and method for extracting membrane-bound target molecules
US8008475B1 (en) 1997-10-23 2011-08-30 Qiagen Gmbh Method for isolating and purifying nucleic acids on surfaces
EP1960521B1 (de) * 2005-12-07 2011-09-21 AJ Innuscreen GmbH Verfahren und testkit zur trennung, aufreinigung und wiedergewinnung von lang- und kurzkettigen nukleinsäuren
WO2018033382A1 (en) * 2016-08-15 2018-02-22 F. Hoffmann-La Roche Ag Method of isolating nucleic acids for long sequencing reads

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EP1109899B1 (en) * 1998-09-10 2007-02-21 WHATMAN plc Product and method for separation of a sample containing multiple sources of genetic material using a solid medium
CA2299119C (en) 1999-02-23 2013-02-05 Qiagen Gmbh A method of stabilizing and/or isolating nucleic acids
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
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DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
CN100487116C (zh) * 2001-07-09 2009-05-13 旭化成株式会社 应用非织造织物纯化和检测核酸的方法
DE10147439B4 (de) * 2001-09-26 2014-01-30 Qiagen Gmbh Verfahren zur Isolierung von DNA aus biologischen Proben
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
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EP2163620A1 (de) 2008-09-03 2010-03-17 Qiagen GmbH Verfahren zum Isolieren und Reinigen von Nukleinsäuren
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DE102010043015B4 (de) * 2010-10-27 2019-12-19 Robert Bosch Gmbh Verfahren zur Konzentration von Probenbestandteilen und Amplifikation von Nukleinsäuren
EP2742152B1 (en) 2011-08-12 2017-04-12 Qiagen GmbH Method for isolating nucleic acids
USD683044S1 (en) * 2011-12-21 2013-05-21 Curetis Ag Column adapter
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US9399986B2 (en) 2012-07-31 2016-07-26 General Electric Company Devices and systems for isolating biomolecules and associated methods thereof
EP2888363B1 (en) * 2012-08-21 2018-06-06 Qiagen GmbH Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
CN103131628A (zh) * 2013-02-04 2013-06-05 上海交通大学 植物基因组dna快速提取装置及其检测方法
US20140272967A1 (en) * 2013-03-13 2014-09-18 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
JP6431522B2 (ja) * 2013-03-15 2018-11-28 アボツト・モレキユラー・インコーポレイテツド 核酸の精製のための１工程法
PT3134506T (pt) 2014-04-25 2019-10-31 Translate Bio Inc Métodos de purificação de rna mensageiro
DE102014216016A1 (de) 2014-08-13 2016-02-18 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren
DE102014118532B4 (de) * 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
JP6815334B2 (ja) 2015-06-09 2021-01-20 バイオカーティス エヌ ヴイ 核酸の単離のための自動化可能な方法
CN108138366A (zh) 2015-07-23 2018-06-08 比奥卡尔齐斯股份有限公司 优化的临床样品测序
WO2018128585A1 (en) * 2017-01-04 2018-07-12 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
US11199476B2 (en) 2017-01-13 2021-12-14 Luna Innovations Incorporated Dissolvable nanofiber materials and specimen recovery kits including the same for high efficiency specimen recovery
US20230134238A1 (en) * 2020-03-12 2023-05-04 The Regents Of The University Of California Well plate and petri dish fluid exchange plug
KR20230169935A (ko) * 2021-01-12 2023-12-18 데피니티브 바이오테크놀로지스 엘엘씨 생물학적 시료들에서 핵산을 검출하기 위한 디바이스및 방법
KR102291738B1 (ko) * 2021-01-15 2021-08-20 (주)진이어스 컬럼 방식의 단일튜브 pcr용 핵산 분리를 위한 버퍼 조성물 및 이의 용도
KR102425034B1 (ko) * 2021-12-14 2022-07-27 주식회사 에이아이더뉴트리진 표적 바이러스 검출을 위한 페이퍼 멤브레인 기반 현장용 rna 추출 플랫폼

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US8008475B1 (en) 2011-08-30
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US20100159460A1 (en) 2010-06-24
US20100021925A1 (en) 2010-01-28
AU1231899A (en) 1999-05-17
JP4440458B2 (ja) 2010-03-24
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