AU1231899A - Method for isolating and purifying nucleic acids on surfaces - Google Patents

Method for isolating and purifying nucleic acids on surfaces

Info

Publication number
AU1231899A
AU1231899A AU12318/99A AU1231899A AU1231899A AU 1231899 A AU1231899 A AU 1231899A AU 12318/99 A AU12318/99 A AU 12318/99A AU 1231899 A AU1231899 A AU 1231899A AU 1231899 A AU1231899 A AU 1231899A
Authority
AU
Australia
Prior art keywords
nucleic acids
isolating
purifying nucleic
optionally
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU12318/99A
Other languages
English (en)
Inventor
Helge Bastian
Simone Gauch
Uwe Oelmuller
Susanne Ullmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of AU1231899A publication Critical patent/AU1231899A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Detergent Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Cleaning Or Drying Semiconductors (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
AU12318/99A 1997-10-23 1998-10-23 Method for isolating and purifying nucleic acids on surfaces Abandoned AU1231899A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19746874A DE19746874A1 (de) 1997-10-23 1997-10-23 Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
DE19746874 1997-10-23
PCT/EP1998/006756 WO1999022021A1 (de) 1997-10-23 1998-10-23 Verfahren zur isolierung und reinigung von nukleinsäuren an oberflächen
EP99923428A EP1121460B1 (de) 1997-10-23 1999-04-20 Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen

Publications (1)

Publication Number Publication Date
AU1231899A true AU1231899A (en) 1999-05-17

Family

ID=85514527

Family Applications (1)

Application Number Title Priority Date Filing Date
AU12318/99A Abandoned AU1231899A (en) 1997-10-23 1998-10-23 Method for isolating and purifying nucleic acids on surfaces

Country Status (7)

Country Link
US (5) US8008475B1 (https=)
EP (3) EP1049801B1 (https=)
JP (3) JP4440458B2 (https=)
AT (1) ATE335846T1 (https=)
AU (1) AU1231899A (https=)
DE (2) DE19746874A1 (https=)
WO (2) WO1999022021A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000015776A1 (en) * 1998-09-10 2000-03-23 Flinders Technologies Pty. Ltd. Product and method for separation of a sample containing multiple sources of genetic material using a solid medium

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7683035B1 (en) 1999-02-23 2010-03-23 Qiagen, Gmbh Method of stabilizing and/or isolating nucleic acids
US6423536B1 (en) * 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
KR100758323B1 (ko) * 2000-02-15 2007-09-12 바이오 스트랜드 인코포레이티드 검출용 물질 배치 장치, 검출용 물질 배치용 필름 및 검출용 물질 지지체의 제조 방법
DE10122990A1 (de) * 2001-05-11 2002-11-14 Qiagen Gmbh Verwendung von Alkanen zur kontaminationsfreien Reinigung bzw. Trennung von Biopolymeren
JPWO2003006650A1 (ja) * 2001-07-09 2004-11-04 旭化成株式会社 不織布を用いた核酸精製法及び検出法
DE10147439B4 (de) * 2001-09-26 2014-01-30 Qiagen Gmbh Verfahren zur Isolierung von DNA aus biologischen Proben
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US6897027B2 (en) * 2002-03-27 2005-05-24 Decode Genetics Ehf. Method for desalting nucleic acids
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
US7759055B2 (en) 2002-08-28 2010-07-20 Millipore Corporation Compositions of solution for sequencing reaction clean-up
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US20040245163A1 (en) * 2003-06-06 2004-12-09 Gary Lim Purification device for ribonucleic acid in large volumes, and method
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
US7031802B2 (en) * 2003-08-13 2006-04-18 Hewlett-Packard Development Company, L.P. Semi-autonomous operation of a robotic device
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
EP1526176A3 (en) * 2003-10-24 2005-05-11 Agilent Technologies Inc. (a Delaware Corporation) Devices and methods for isolating RNA
WO2005058453A1 (en) * 2003-12-15 2005-06-30 Preentec Ag Method for the concentration and purification of biological compounds
EP1548126A1 (en) * 2003-12-22 2005-06-29 Bio-Rad Pasteur Solid support for control nucleic acid, and application thereof to nucleic acid detection
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1727900A4 (en) * 2004-03-26 2008-05-28 Fujifilm Corp METHOD FOR SELECTIVELY SEPARATING AND CLEANING RNA, AND METHOD FOR SEPARATING AND CLEANING NUCLEIC ACID
WO2005118803A1 (ja) * 2004-06-02 2005-12-15 Arkray, Inc. 核酸抽出用容器、固体マトリックスの洗浄方法および洗浄機構、ならびに核酸精製方法
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
US7326386B2 (en) * 2005-01-31 2008-02-05 Fujifilm Corporation Apparatus for extracting nucleic acid
PL1871805T3 (pl) 2005-02-07 2020-03-31 Roche Glycart Ag Cząsteczki wiążące antygen, które wiążą egfr, wektory kodujące te cząsteczki oraz ich zastosowania
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
DE102005040259A1 (de) * 2005-08-24 2007-03-01 Qiagen Gmbh Verfahren zur Gewinnung von Nukleinsäuren aus Blut
DE102005059217B4 (de) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
US20070190535A1 (en) * 2006-02-13 2007-08-16 Hall Gerald E Jr Size fractionation of nucleic acid samples
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
EP1938756A1 (en) * 2006-12-29 2008-07-02 Qiagen GmbH Method and materials for triggered release of a biological sample
TW200907066A (en) * 2007-08-13 2009-02-16 Taigen Bioscience Corp Method for washing a column and method for extracting membrane-bound target molecules
EP2163620A1 (de) 2008-09-03 2010-03-17 Qiagen GmbH Verfahren zum Isolieren und Reinigen von Nukleinsäuren
GB0818609D0 (en) * 2008-10-10 2008-11-19 Univ Hull apparatus and method
WO2011157678A1 (en) * 2010-06-14 2011-12-22 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
EP2407540A1 (en) * 2010-07-15 2012-01-18 Qiagen GmbH Method for purifying a target nucleic acid
DE102010043015B4 (de) * 2010-10-27 2019-12-19 Robert Bosch Gmbh Verfahren zur Konzentration von Probenbestandteilen und Amplifikation von Nukleinsäuren
EP3789500A1 (en) 2011-08-12 2021-03-10 QIAGEN GmbH Method for isolating nucleic acids
USD683044S1 (en) * 2011-12-21 2013-05-21 Curetis Ag Column adapter
GB2561425B (en) 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
US9399986B2 (en) 2012-07-31 2016-07-26 General Electric Company Devices and systems for isolating biomolecules and associated methods thereof
EP2888363B1 (en) * 2012-08-21 2018-06-06 Qiagen GmbH Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
CN103131628A (zh) * 2013-02-04 2013-06-05 上海交通大学 植物基因组dna快速提取装置及其检测方法
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法
EP3828284A1 (en) * 2013-03-15 2021-06-02 Abbott Molecular Inc. One-step procedure for the purification of nucleic acids
US9850269B2 (en) 2014-04-25 2017-12-26 Translate Bio, Inc. Methods for purification of messenger RNA
DE102014216016A1 (de) 2014-08-13 2016-02-18 Axagarius Gmbh & Co. Kg Vorrichtung zur Aufreinigung von Nukleinsäuren
DE102014118532B4 (de) * 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
WO2016198519A1 (en) * 2015-06-09 2016-12-15 Biocartis N.V. Automatable method for nucleic acid isolation
WO2017013102A1 (en) 2015-07-23 2017-01-26 Biocartis Nv Optimized clinical sample sequencing
EP3497216B1 (en) * 2016-08-15 2022-10-19 F. Hoffmann-La Roche AG Method of isolating nucleic acids for long sequencing reads
US20190329244A1 (en) * 2017-01-04 2019-10-31 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
WO2018132244A1 (en) * 2017-01-13 2018-07-19 Luna Innovations Incorporated Dissolvable nanofiber materials and specimen recovery kits including the same for high efficiency specimen recovery
WO2021183889A1 (en) * 2020-03-12 2021-09-16 The Regents Of The University Of California Well plate and petri dish fluid exchange plug
US12390808B2 (en) * 2021-01-12 2025-08-19 Definitive Biotechnologies Llc Device and method for detecting nucleic acids in biological samples
KR102291738B1 (ko) * 2021-01-15 2021-08-20 (주)진이어스 컬럼 방식의 단일튜브 pcr용 핵산 분리를 위한 버퍼 조성물 및 이의 용도
KR102425034B1 (ko) * 2021-12-14 2022-07-27 주식회사 에이아이더뉴트리진 표적 바이러스 검출을 위한 페이퍼 멤브레인 기반 현장용 rna 추출 플랫폼

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482834A (en) * 1982-05-17 1996-01-09 Hahnemann University Evaluation of nucleic acids in a biological sample hybridization in a solution of chaotrophic salt solubilized cells
US4798789A (en) * 1984-10-05 1989-01-17 Dnax Research Institute Of Molecular And Cellular Biology, Inc. cDNA clones coding for polypeptides exhibiting murine interleukin-2 activity
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US5258283A (en) * 1985-11-05 1993-11-02 Battelle Memorial Institute Detection and differentiation of coxiella burnetii in biological fluids
EP0245945B1 (en) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automated nucleic acid extractor
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
DE3781217T2 (de) * 1986-07-22 1992-12-17 Ciba Geigy Ag Herstellung von verwandten polypeptiden des bindungsfaktors.
US4833239A (en) * 1987-01-29 1989-05-23 Genetics Institute, Inc. Method for the isolation and purification of DNA molecules
DE3874010T2 (de) * 1987-06-12 1993-01-07 Pall Corp Diagnostische testvorrichtung.
US5004543A (en) * 1988-06-21 1991-04-02 Millipore Corporation Charge-modified hydrophobic membrane materials and method for making the same
US5252293A (en) * 1989-01-17 1993-10-12 Vladimir Drbal Analytical slide with porous filter membrane
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JP2978518B2 (ja) * 1989-12-06 1999-11-15 東ソー株式会社 ファージｄｎａの精製方法
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
US5459015A (en) * 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
JPH04187077A (ja) * 1990-11-22 1992-07-03 Shimadzu Corp 核酸の抽出精製装置
JPH04325099A (ja) * 1991-04-25 1992-11-13 Mitsui Petrochem Ind Ltd 核酸の膜への固定法
CA2067712A1 (en) * 1991-05-03 1992-11-04 Daniel L. Woodard Filtration purification of dna
US6028186A (en) * 1991-06-10 2000-02-22 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands of cytokines
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5308580A (en) * 1992-05-19 1994-05-03 Millipore Corporation Sample collection and analytical device
DE69326507T2 (de) * 1992-07-02 2000-04-27 Edge Biosystems Inc., Gaithersburg Verfahren und Material für Reinigung von Nukleinsäuren
DE69223617T2 (de) * 1992-09-18 1998-04-09 Amersham Int Plc Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
US5527672A (en) * 1993-02-24 1996-06-18 Millipore Investment Holdings Limited Hydrophobic coated membranes
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
JPH0751099A (ja) * 1993-08-11 1995-02-28 Toyobo Co Ltd 核酸配列検査方法及び検査装置
CA2170604C (en) * 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
US5438127A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
GB9322650D0 (en) * 1993-11-03 1993-12-22 Cogent Diagnostics Ltd Analytical device
EP0891712A1 (en) * 1993-12-20 1999-01-20 Biopolymerix, Inc. Liquid dispenser for sterile solutions
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US6180778B1 (en) 1994-02-11 2001-01-30 Qiagen Gmbh Process for the separation of double-stranded/single-stranded nucleic acid structures
JP3093116B2 (ja) * 1994-09-30 2000-10-03 株式会社豊田中央研究所 核酸検出方法
US5736351A (en) * 1995-01-09 1998-04-07 New Horizons Diagnostics Corporation Method for detection of contaminants
DE19512361A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh Verfahren zur Isolierung eines biologischen Materials
KR19990022612A (ko) * 1995-06-08 1999-03-25 퍼니스 챨스 엠 디엔에이 추출방법 및 추출장치
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US6146854A (en) * 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
EP0814156B1 (en) * 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Method for the purification of DNA
DE19702907A1 (de) * 1997-01-28 1998-07-30 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
DE69829466T2 (de) * 1997-03-24 2006-01-26 Fields, Robert E., Menlo Park Biomolekularer prozessor
US5858700A (en) * 1997-04-03 1999-01-12 Kemin Foods, Lc Process for the isolation and purification of lycopene crystals
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000015776A1 (en) * 1998-09-10 2000-03-23 Flinders Technologies Pty. Ltd. Product and method for separation of a sample containing multiple sources of genetic material using a solid medium

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EP2251440A3 (de) 2011-11-16
DE59813680D1 (de) 2006-09-21
JP4440458B2 (ja) 2010-03-24
EP1049801A1 (de) 2000-11-08
WO1999022021A1 (de) 1999-05-06
JP2008245649A (ja) 2008-10-16
EP1121460B1 (de) 2010-07-14
US20050244882A1 (en) 2005-11-03
US20030152974A1 (en) 2003-08-14
DE19746874A1 (de) 1999-04-29
EP1121460A1 (de) 2001-08-08
EP2251440A2 (de) 2010-11-17
US20100159460A1 (en) 2010-06-24
EP1049801B1 (de) 2006-08-09
JP2001520894A (ja) 2001-11-06
ATE335846T1 (de) 2006-09-15
US8008475B1 (en) 2011-08-30
JP2008263978A (ja) 2008-11-06
US20100021925A1 (en) 2010-01-28
WO2000024927A1 (de) 2000-05-04

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