WO2002083931A3 - Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen - Google Patents

Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen Download PDF

Info

Publication number
WO2002083931A3
WO2002083931A3 PCT/EP2002/004042 EP0204042W WO02083931A3 WO 2002083931 A3 WO2002083931 A3 WO 2002083931A3 EP 0204042 W EP0204042 W EP 0204042W WO 02083931 A3 WO02083931 A3 WO 02083931A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemically inert
onto chemically
fixing
biomolecules
inert surfaces
Prior art date
Application number
PCT/EP2002/004042
Other languages
English (en)
French (fr)
Other versions
WO2002083931A2 (de
Inventor
Hans-Willi Kling
Jens Seemann
Johannes Georg Schindler
Original Assignee
Henkel Kgaa
Hans-Willi Kling
Jens Seemann
Johannes Georg Schindler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2001118553 external-priority patent/DE10118553A1/de
Priority claimed from DE2001118554 external-priority patent/DE10118554A1/de
Application filed by Henkel Kgaa, Hans-Willi Kling, Jens Seemann, Johannes Georg Schindler filed Critical Henkel Kgaa
Priority to EP02732600A priority Critical patent/EP1379680A2/de
Priority to US10/474,769 priority patent/US20040175811A1/en
Priority to JP2002582268A priority patent/JP2004533238A/ja
Publication of WO2002083931A2 publication Critical patent/WO2002083931A2/de
Publication of WO2002083931A3 publication Critical patent/WO2002083931A3/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes

Abstract

Es wird ein Verfahren zur Anbindung von Biomolekülen, insbesondere Enzymen oder enzymatischen Systemen, an plasmachemisch aktivierte bzw. funktionalisierte, chemisch inerte Trägeroberflächen beschrieben. Insbesondere wird ein Verfahren zur Immobilisierung von Biomolekülen, vorzugsweise Enzymen oder enzymatischen Systemen, durch direkte oder mittelbare Anbindung, insbesondere chemische und/oder physikalische Anbindung, an plasmachemisch aktivierte bzw. funktionalisierte, chemisch inerte Trägeroberflächen beschrieben. Die auf diese Weise immobilisierten Biomoleküle eignen sich beispielsweise zur Anwendung in Bioreaktoren, Biosensoren und chromatographischen Systemen.
PCT/EP2002/004042 2001-04-14 2002-04-11 Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen WO2002083931A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02732600A EP1379680A2 (de) 2001-04-14 2002-04-11 Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen
US10/474,769 US20040175811A1 (en) 2001-04-14 2002-04-11 Method for fixing biomolecules onto chemically inert surfaces
JP2002582268A JP2004533238A (ja) 2001-04-14 2002-04-11 化学的に不活性な表面上に生体分子を固定する方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE2001118553 DE10118553A1 (de) 2001-04-14 2001-04-14 Verfahren zur Anbindung von Biomolekülen an chemisch inerte Oberflächen
DE2001118554 DE10118554A1 (de) 2001-04-14 2001-04-14 Enzymabbauketten
DE10118554.5 2001-04-14
DE10118553.7 2001-04-14

Publications (2)

Publication Number Publication Date
WO2002083931A2 WO2002083931A2 (de) 2002-10-24
WO2002083931A3 true WO2002083931A3 (de) 2003-07-31

Family

ID=26009089

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2002/004043 WO2002083932A2 (de) 2001-04-14 2002-04-11 Enzymabbauketten
PCT/EP2002/004042 WO2002083931A2 (de) 2001-04-14 2002-04-11 Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/004043 WO2002083932A2 (de) 2001-04-14 2002-04-11 Enzymabbauketten

Country Status (4)

Country Link
US (2) US20040175811A1 (de)
EP (2) EP1379680A2 (de)
JP (1) JP2004533238A (de)
WO (2) WO2002083932A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4632400B2 (ja) * 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
JP2005289931A (ja) * 2004-04-02 2005-10-20 Tokai Univ 中空状物品
JP2006094812A (ja) * 2004-09-30 2006-04-13 Mitsubishi Kagaku Iatron Inc バイオリアクター用担体、バイオリアクター、及び分析方法
JP4649680B2 (ja) * 2005-03-02 2011-03-16 独立行政法人産業技術総合研究所 酵素固定化マイクロリアクター、及びその製造方法
CN100363482C (zh) * 2005-12-09 2008-01-23 清华大学 利用亲水/疏水复合膜中的微结构固定化脂肪酶的方法
JP4727543B2 (ja) * 2006-09-27 2011-07-20 富士フイルム株式会社 測定装置及び測定方法
IT1394278B1 (it) * 2009-06-17 2012-06-06 Archimede R&D S R L Metodo per la prevenzione ed il controllo di organismi infestanti i sistemi acquosi
CN101935669B (zh) * 2009-12-16 2013-01-02 中国科学院动物研究所 一种解毒工程菌及其制备方法和应用
EP3085463A1 (de) * 2014-12-16 2016-10-26 Luxembourg Institute of Science and Technology (LIST) Verfahren zum abbau und zur deaktivierung von antibiotika in wasser durch immobilisierte enzyme auf funktionalisierten trägern

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0104608A1 (de) * 1982-09-24 1984-04-04 Becton Dickinson and Company Chemisch modifizierte Oberfläche zur Befestigung grosser Moleküle
JPS59203951A (ja) * 1983-05-06 1984-11-19 Kuraray Co Ltd 生物電気化学センサ−の製造方法
JPS63283582A (ja) * 1987-05-14 1988-11-21 Nok Corp 酵素固定化ポリアクリロニトリル膜の製造法
US5334296A (en) * 1992-01-15 1994-08-02 Andcare, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
US5545317A (en) * 1992-12-30 1996-08-13 Dow Corning Corporation Liquid column packing materials and method for making the same
DE19835869A1 (de) * 1998-08-07 2000-02-17 Fraunhofer Ges Forschung Verfahren zur Herstellung von stabilen Enzymschichten
JP2000298111A (ja) * 1999-04-15 2000-10-24 Sentan Kagaku Gijutsu Incubation Center:Kk バイオセンサー
WO2001055248A2 (en) * 2000-01-27 2001-08-02 Wisconsin Alumni Research Foundation Process for the attachment of spacer molecules for binding active biomolecules to substrates
WO2002053299A1 (en) * 2000-12-29 2002-07-11 Nkt Research & Innovation A/S A method for the preparation of a substrate for immobilising chemical compounds and the substrate and the use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3640877A (en) * 1969-04-17 1972-02-08 Michael R R Gobert Detergent
DE3326546A1 (de) * 1983-07-22 1985-02-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., 8000 München Verfahren zur kontinuierlichen enzymatischen herstellung von gluconsaeure oder ihren derivaten und sorbit und/oder mannit
JPS6093344A (ja) * 1983-10-27 1985-05-25 Mitsubishi Petrochem Co Ltd グルコ−スおよびα−アミラ−ゼ活性の測定法
JPS6131954A (ja) * 1984-07-25 1986-02-14 Hitachi Ltd マルト−スセンサ
JPS61124399A (ja) * 1984-11-19 1986-06-12 Shimadzu Corp 糖類の分析方法及び装置
JPS62277549A (ja) * 1986-05-27 1987-12-02 Fuji Electric Co Ltd α−アミラ−ゼの定量装置
DD294344A5 (de) * 1990-05-03 1991-09-26 Veb Filmfabrik Wolfen,De Analytisches element zur gleichzeitigen bestimmung von oligosaccariden und glucose

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0104608A1 (de) * 1982-09-24 1984-04-04 Becton Dickinson and Company Chemisch modifizierte Oberfläche zur Befestigung grosser Moleküle
JPS59203951A (ja) * 1983-05-06 1984-11-19 Kuraray Co Ltd 生物電気化学センサ−の製造方法
JPS63283582A (ja) * 1987-05-14 1988-11-21 Nok Corp 酵素固定化ポリアクリロニトリル膜の製造法
US5334296A (en) * 1992-01-15 1994-08-02 Andcare, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
US5545317A (en) * 1992-12-30 1996-08-13 Dow Corning Corporation Liquid column packing materials and method for making the same
DE19835869A1 (de) * 1998-08-07 2000-02-17 Fraunhofer Ges Forschung Verfahren zur Herstellung von stabilen Enzymschichten
JP2000298111A (ja) * 1999-04-15 2000-10-24 Sentan Kagaku Gijutsu Incubation Center:Kk バイオセンサー
WO2001055248A2 (en) * 2000-01-27 2001-08-02 Wisconsin Alumni Research Foundation Process for the attachment of spacer molecules for binding active biomolecules to substrates
WO2002053299A1 (en) * 2000-12-29 2002-07-11 Nkt Research & Innovation A/S A method for the preparation of a substrate for immobilising chemical compounds and the substrate and the use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GANAPATHY R ET AL: "Immobilization of active alpha-Chymotrypsin on RF-plasma-functionalized polymer surfaces", JOURNAL OF APPLIED POLYMER SCIENCE, JOHN WILEY AND SONS INC. NEW YORK, US, vol. 78, no. 10, 5 December 2000 (2000-12-05), pages 1783 - 1796, XP002177429, ISSN: 0021-8995 *
PATENT ABSTRACTS OF JAPAN vol. 009, no. 072 (P - 345) 2 April 1985 (1985-04-02) *
PATENT ABSTRACTS OF JAPAN vol. 013, no. 107 (C - 576) 14 March 1989 (1989-03-14) *
PATENT ABSTRACTS OF JAPAN vol. 2000, no. 13 5 February 2001 (2001-02-05) *

Also Published As

Publication number Publication date
EP1385985A2 (de) 2004-02-04
JP2004533238A (ja) 2004-11-04
WO2002083932A2 (de) 2002-10-24
US20040209340A1 (en) 2004-10-21
US20040175811A1 (en) 2004-09-09
EP1379680A2 (de) 2004-01-14
WO2002083932A3 (de) 2003-11-27
WO2002083931A2 (de) 2002-10-24

Similar Documents

Publication Publication Date Title
AU1231899A (en) Method for isolating and purifying nucleic acids on surfaces
TW200712491A (en) Adhesive bead for immobilization of biomolecules and method for fabricating a biochip using the same
WO2002086083A3 (en) Methods of enhancing cell responsiveness
WO2006058237A3 (en) Polymer-coated substrates for binding biomolecules and methods of making and using thereof
AU2002322458A1 (en) Method for immobilizing molecules onto surfaces
WO2000032809A3 (de) Kopieren und klonieren an oberflächen
WO2002083931A3 (de) Verfahren zur anbindung von biomolekülen an chemisch inerte oberflächen
WO2002083933A3 (de) Verfahren zur bestimmung der substratspezifität einer enzymatischen aktivität und vorrichtung hierzu
WO2001002600A3 (en) Detection of analytes using attenuated enzymes
EP1293779A3 (de) Biosensoroberfläche
WO2007012605A3 (en) Static support bed for purification, separation, detection, modification and/or immobilization of target entities and method using thereof
WO2002014830A3 (en) Method of identification and quantification of biological molecules and apparatus therefore
AU2002368411A1 (en) Method for immobilizing biologic molecules on solid surfaces
AU2002304623A1 (en) Method for reducing an adsorption tendency of molecules or biological cells on a material surface
WO2002002795A3 (de) Multiplex-sequenzierungsverfahren
WO2003048310A3 (en) Thermus oshimai nucleic acid polymerases
AU1856300A (en) Derivatisation of support surfaces
WO2001000846A3 (en) Linoleate isomerase
WO2006032809A3 (fr) Procede de detection de streptococcus agalactiae en utilisant l'activite esterase
AU2003269447A1 (en) Biosensor and method comprising enzymes immobilized on semiconductors
EP1130121A3 (de) Immobilisierte Nukleinsäuren und Methoden zum Nchhweis von Nukleinsäuren
WO2004011401A3 (fr) Procede de fabrication de puces biologiques
AU2003229645A1 (en) Method for immobilizing molecules on surfaces
WO2005010086A3 (en) Immobilization methods for organic molecules telomers and polymers on solid substrates
WO2003052137A3 (de) Evaneszenz-basierendes multiplex-sequenzierungsverfahren

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU BG BR BY CA CN CZ DZ HU ID IL IN JP KR MX NO NZ PL RO RU SG SI SK UA US UZ VN YU ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002732600

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002582268

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2002732600

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10474769

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 2002732600

Country of ref document: EP